• 한국식품영양과학회지
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• 제30권1호
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• pp.152-158
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• 2001

In this study, we determined major foods and food groups which contributed to the intake of folate in korean child-bearing women. Folate content of the major foods was also analyzed. A total of 91 healthy women with childbearing potential participated. They were divided into one of three groups by age; A (15∼24 yrs), B(25∼34 yrs) and C (35∼49 yrs). Food consumption data of the subjects were obtained by the 24-hr recall method. The rate of contribution of each food to the total intake of dietary folate was determined. Seventy major foods for folate were selected from the 178 foods they consumed. Two sets of the 70 foods were collected from different markets. After extracting folates from the foods by heating with a HEPES-CHES buffer, the samples were treated with α-amylase, protease and folate intakes was Korean cabbage kimchi followed by laver, soybean sprout, rice, yolmu kimchi, eggs, lettuce, perilla leaves, sea mustard and radish root. Food items that contributed to folate intake were slightly different among the age groups. Although the folate content of Korean cabbage kimchi was 51.6 ㎍/100g, but due to the high amount of intake, it was ranked the first major food prviding folate. High folate foods containing over 100㎍/100g were laver, corn flakes, spinach, mungbean, sesame, quail's eggs, small radish kimchi, kidney beans, leeks and peanuts. However, based on the serving size, spinach, corn flakes, leeks, sweet potato, pepper leaves, quail's eggs, crown daisy, small radish kimchi and perilla leaves contributed above 50㎍ of folate per serving size.

• Journal of Nutrition and Health
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• 제48권1호
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• pp.94-104
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• 2015

본 연구에서는 엽산의 급원식품으로 보고된 식품 51종의 엽산을 분석하고, 값에 차이가 많이 있는 경우 엽산 데이터베이스를 수정하여, 수정한 데이터베이스로 어린이와 청소년의 엽산 섭취량을 추산하고 급원식품을 알아보고자 하였다. 대상자는 대전, 충청도 및 전라도에 거주하는 만 20세 미만 567명으로 비연속 2일 동안의 24시간 회상법에 의해 식품섭취조사를 실시하였다. 51종의 식품을 새롭게 분석하여 현재의 데이터베이스와 비교한 결과 현재 값의 18.7%~322.9%의 결과를 얻었다. 기존의 값이 실험한 두 값 사이에 있거나, 기존값과의 차이가 10% 미만인 경우에는 수정하지 않았으며, 그 외에 차이가 있는 40종은 새로운 분석값으로 수정하였다. 수정한 데이터베이스로 대상자들의 엽산 평균섭취량을 성별, 연령별로 나누어 계산한 결과 모든 연령층에서 평균 엽산 섭취량이 권장섭취량보다 높았으나 12~14세 남자와 12~19세 여자의 평균 엽산 섭취량은 권장섭취량보다 낮은 것으로 나타났다. 계란, 배추김치, 쌀, 귤은 모든 연령층에서 엽산 급원식품의 10위 안에 포함되었다. 본 연구에서는 일부 급원식품 51종만을 분석하였으나 한국인들이 자주 먹는 식품의 조리 후 엽산 함량을 분석하여 보완해 나감으로써 더 정확한 엽산 섭취량을 추산할 수 있도록 해야 할 것이다. 또한 12~19세 여자의 평균 엽산 섭취량이 다른 연령층에 비해 상대적으로 낮게 나타난 것을 볼 수 있었는데, 엽산은 성장기 및 가임여성에게 특히 중요한 비타민으로써 이들을 위한 엽산 영양교육이 이루어져야 할 것으로 생각된다.

• Journal of Nutrition and Health
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• 제38권7호
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• pp.586-604
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• 2005

A reliable nutrient database is a prerequisite for accurate calculation of dietary intakes. The folate database currently available in Korea, however, is not reliable because the values were obtained from published data in other countries using ineffective methods to extract folates from the food matrix. The purpose of this study was to complement the folate database by analyzing folate content in foods using a more effective method to extract food folates (trienzyme treatment). Folate content per unit weight was highest in laver, fermented soybeans, soybean, spinach, black soybeans, crown daisy, mung beans, and quail's egg in descending order. Legumes, leafy greens, eggs, and seaweeds were rich in folate, and meats, chicken, fish, and some fruits contained less folate. Some of the analyzed values were 10 times higher than those in the currently available database. Folate values of 423 foodcodes out of 2,932 foodcodes ($14.4\%$) in the database in the 7th revision in the Recommended Dietary Allowances for Koreans can be replaced by those analyzed in this study. Since folate values of rice and Kimchi, which are core dishes of Koreans, in the newly established database are higher than those in the current database, folate intake assessed using our data will be higher than that using the current available database. Folate content in more foods commonly consumed in Korea are needed to update the folate database. Meanwhile, folate values presented here can be used to assess dietary folate intake of the Korean population.

• Journal of Nutrition and Health
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• 제12권2호
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• pp.53-63
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• 1979

한국인의 식생활을 이루고 있는 주요식품 226종에 포함된 엽산치를 Lactobacillus casei 미생들을 사용하여 분석하였다. 제 1보 및 2보에서 측정된 채소류와 과실류 식품은 이 보고에서 제외되었다. 엽산치함량은 유리형 및 결합형으로 각종 식품 100g중에 포함된 ug으로 표시하였다. 각종 식품 및 각 식품군에 포함된 영산치 함량은 크게 차이가 있는 것으로 나타났다. 또한 각종 식품에 포참된 영산 형태에 따른 화학적 조성도 크게 차이가 있는 것으로 나타났다. 식단 작성시 결합형의 엽산치 함량이 사용될 것이며, 저장가공 및 조리계의 파괴율을 고려해야 하겠다. 가장 풍부한 영산의 급원은 효모로 2,800.8ug 결합형으로 식품 100g중에 함유됨을 보였다. 밀린 대두, 시금치, 냉이, 소 및 돼지 같은 100ug이상 함유됨을 보였다. 그 외의 말린 두류 및 종실류, 꼭, 숙갓, 미나리, 부추, 아스파라가스, 계란노른자, 소 콩팥은 50ug 이상 함유됨을 보였다. 파, 상치, 무우, 고추, 발효된 중 식품, 밀, 고구마, 느타리 버섯은 40 ug정도 함유됨을 보였다. 그외 대부분의 정제된 곡류, 전분류, 당류, 과실류, 육류, 어패류, 유류는 비교적 적게 함유됨을 보였다. 유지류, 음로 및 주류 조미료는 거의 함유량이 없음을 보였다. 저장가공 및 조리된 식품에서의 엽산치 함량은 전반적으르 크게 손실될 것으로 나타났다.

• Journal of Nutrition and Health
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• 제33권3호
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• pp.296-303
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• 2000

We examined the folate intakes and assessed folate nutritional status of Korean women with childbearing potential. A total of 91 healthy women aged between 15 and 49 participated. They were divided into three groups by their age : A(15-24 yrs), B(25-34 yrs) and C(35-49 yrs). Folate intakes were determined by direct analysis. The foods consumed for 24 hours were collected proportionally and assessed folate. Their blood drawn in fasting state were analyzed folate levels. Folate contents of food homogenate, plasma and erythrocyte were determined a microbiological method using Lactobacillus. casei (ATCC 7469). Prior to the micro-assay, the food homogenate were treated with alpha-amylase, protease and folate conjugase. Mean daily folate intake of the total subjects was 145.8$\mu\textrm{g}$/d and in each group of A, B, and C was 114.0$\mu\textrm{g}$/d, 141.6$\mu\textrm{g}$/d, and 164.6$\mu\textrm{g}$/d, respectively. That of group C was significantly higher than that of group A(p<0.05). However, those of all the groups were lower than compared to the Korean Recommened Dietary Allowances(RDA) for folate. Especially the subjects in the group A consumed folate least that was below the half of the Korean RDA. The mean energy intake of all subjects was 1638㎉/d and those in each group of A, B, and C did not meet the Korean RDA for energy. The energy intake were significantly correlated with folate intakes(r=0.5050, p<0.001). Mean plasma and erythrocyte folate concentrations of total subjects were 6.9ng/mL and 266.3ng/mL, respectively. None were found to be deficient both in plasma(<3ng/mL)and erythrocyte (<140ng/mL) folate levels. There was only one subject who had red blood cell folate level below 157ng/mL concentration. These results show that folate status of the Korean women of reproductive age is not much bad. But it should be better that letting them improve their folate status by increasing energy intake, choosing high folate foods.

• Journal of Nutrition and Health
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• 제42권4호
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• pp.397-405
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• 2009

본 연구에서는 식품 중의 엽산을 추출하기 위한 trienzyme 방법을 개선하기 위하여 ${\alpha}$-amylase와 folate conjugase 를 동시에 처리하는 방법 (CI)과 기존의 방법대로 protease, ${\alpha}$-amylase, folate conjugase 세가지 효소를 각각 처리하는 방법 (SI)을 비교해 보았으며, 개선된 방법을 이용하여 패스트푸드와 가공식품에 들어있는 엽산 함량을 분석하였다. 1) 12종 88개 식품에 대하여 엽산을 추출하기 위한 기존의 방법 (SI)과 두가지 효소를 동시에 처리하는 CI 방법으로 엽산 함량을 비교한 결과 식품의 종류에 따라 두 방법간에 -6.5%에서 34.0%까지의 차이를 보였다. 식품의 종류별로 paired t-test를 한 결과 9종에서는 유의적 차이를 볼 수 없었으며, 3종에서는 CI 방법이 SI 방법에 비해 유의적으로 높은 것으로 나타났다. 이는 효소 처리시간이 단축됨으로 인해 엽산의 손실이 적었기 때문인 것으로 보이며, 따라서 실험시간을 절약할 수 있는 CI 방법을 활용하는 것이 바람직하겠다. 2) CI 방법으로 식품 중의 엽산을 추출하고 미생물학적 방법으로 엽산 함량을 분석한 결과 햄버거의 100 g당 평균 엽산 함량은 29.0 ${\mu}g$, 치즈버거 35.6 ${\mu}g$, 불고기버거 34.4 ${\mu}g$, 새우버거 33.5 ${\mu}g$, 치킨버거 29.3 ${\mu}g$, 휘시버거 31.7 ${\mu}g$이었다. 비스킷의 엽산 함량은 28.1 ${\mu}g$, 감자튀김은 49.1 ${\mu}g$이었고, 피자 29.7 ${\mu}g$, 샌드위치 31.6 ${\mu}g$, 삼각김밥 24.3 ${\mu}g$으로 나타났으며, 이 값을 1회 분량으로 환산하면 버거종류는 43.1${\sim}$62.0 ${\mu}g$, 감자튀김 53.3 ${\mu}g$, 피자 28.4 ${\mu}g$, 샌드위치 47.4 ${\mu}g$, 삼각김밥 25.7 ${\mu}g$이었다. 3) 가공식품 26종의 엽산함량을 측정한 결과 100 g 당 엽산 함량이 가장 높은 식품은 캔옥수수로 89.5 ${\mu}g$이었고 다음으로는 냉동만두가 80.8 ${\mu}g$이었다. 그 외의 가공식품으로 소시지는 19.6 ${\mu}g$, 비엔나소시지 11.7 ${\mu}g$, 햄 11.5 ${\mu}g$, 튀긴어묵 10.7 ${\mu}g$ 등으로 측정되어 가공식품의 엽산 함량은 대체로 낮은 수준이었으며, 음료의 엽산 함량은 100 g당 1.0${\sim}$5.2 ${\mu}g$으로 매우 낮았다. 4) 본 연구에서 분석한 패스트푸드와 가공식품, 소스, 음료 등 32종의 엽산 함량을 기존의 식품영양가표와 비교하였을 때 식품영양가표의 엽산값은 분석값과 비슷한 경우도 있었으나 0.04${\sim}$140배 까지 차이가 매우 큰 경우도 있었다. 본 연구에서 분석한 식품 중의 엽산 함량은 우리나라 사람들의 엽산 섭취량을 파악하고자 할 때 기초자료로 활용 될 수 있을 것이며, 어린이, 청소년, 젊은 성인층에서 섭취량이 점차 증가하고 있는 패스트푸드와 새롭게 개발되는 가공식품의 엽산 함량을 앞으로도 지속적으로 분석하여 이를 데이터베이스에 반영할 필요가 있다고 여겨진다.

• Journal of Nutrition and Health
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• 제34권7호
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• pp.797-808
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• 2001

To determine folate intake and food sources in Korea college students, dietary survey was conducted in March, 1999. Dietary data were collected by trained interviewers using the method of 24-hour recalls for 3 consecutive days. The data of 44 male and 62 female students were analyzed with two different nutrient databases in Recommended Dietary Allowances for Korean on the 6th and 7th revisions, and the results were compared. The intakes of energy and nutrients except vitamin A and folate were lower when analyzed with the 7th database than the 6th database. Mean daily folate intakes with the 6th and 7th databases were 172.9$\mu\textrm{g}$, 221.6$\mu\textrm{g}$ for male students while 125.1$\mu\textrm{g}$, 168.0$\mu\textrm{g}$ for female students, respectively. The results showed significantly higher estimates of folate intake with the 7th database, and significantly higher intake in males than females analyzed with both the 6th and 7th databases. Daily folate intake per 1,000kcal (folate density) was 71.1$\mu\textrm{g}$/1000kcal for males and 67.6$\mu\textrm{g}$/1000kcal for females with the 6th database, and 97.9$\mu\textrm{g}$/1000kcal for males and 95.5$\mu\textrm{g}$/1000kcal for females with the 7th database. The differences in folate density between the two databases were signficant, but the differences between the gender were not significant. The proportions of the subjects who consumed more than the RDA of 250$\mu\textrm{g}$ with the 6th and 7th databases were only 4.6%, 29.6% of males and 1.6%, 9.7% of females, respectively. The biggest food source of folate was Kimchi, contributing 17.9% for male and 13.7% for female students with 7th database. Laver, spinach, Ramyon, rice, and Ko Chu Jang together with Kimchi contributed 41.9% for male and 32.4% for females students with the 7th database. These results imply that folate intake reported in the reported inthe previous studies using the 6th database was underestimated. However, the 7th database seems to be still incomplete since 20.5% of 2,932 foods in the database were derived from the other sources, and the rest were imputed from similar foods. Therefore, in order to accurately estimate folate intake of Koreans, folate contents in major contributing foods need to be measured using an appropriate assay method.

• Nutrition Research and Practice
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• 제1권4호
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• pp.254-259
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• 2007

This study assessed folate intakes, folate concentrations in plasma and erythrocytes, plasma total homocysteine (tHcy) concentration, and urinary excretion of folate metabolites in Korean women with childbearing potential. A total of 23 women voluntarily participated in this study. Precise dietary intakes for 3 consecutive days were determined by weighing all foods consumed and folate intake was calculated using a computer-aided dietary analysis system. Folate concentration of plasma and erythrocytes was determined by a microbiological method. Plasma tHcy concentration was assayed using an HPLC analysis method. Urine excreted over the same period of time was collected and folate catabolites, para-aminobenzoylglutamate (pABG) and para-acetamidobenzoylglutamate (ApABG), were evaluated using a reverse-phase HPLC method after affinity chromatography. Young women of 20 and under were likely to consume less folate with low energy intake, had lower folate concentration in plasma and erythrocytes, and excreted a lesser amount of ApABG and total folate catabolites than women of 25 years and over. The results of this study confirmed that young Korean women with childbearing potential, especially those under 21 years of age, might be at risk for compromised folate status due to insufficient folate intakes from inadequate energy consumption.

• Nutrition Research and Practice
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• 제7권3호
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• pp.216-223
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• 2013

The purpose of this study is to assess folate intake, and serum and red blood cell (RBC) folate concentrations, and investigate the association between folate status and health-related behaviors among Korean college students. A total of 169 students, aged between 18 and 27 years, participated in this study. Dietary intake data were collected by trained interviewers using a 24-hour recall method for three non-consecutive days in 2009. Information on health-related behaviors was obtained by a self-administered questionnaire. Serum and RBC folate concentrations were measured by microbiological assay. The average intakes of folate were $456{\mu}gDFE$ and $347{\mu}gDFE$ in male and female students, respectively. While the average serum folate concentration was significantly lower in male students (8.9 ng/mL) compared to female students (12.5 ng/mL), RBC concentrations were not significantly different between male (398.6 ng/mL) and female students (405.3 ng/mL). In male students, low serum folate concentrations were associated with total folate intake less than the Estimated Average Requirement, non-use of folic acid supplements, smoking, alcohol drinking at least once a week and low physical activity. In female students, low serum folate concentrations were associated with smoking and alcohol drinking at least two drinks at a time and BMI ${\geq}25$. Alcohol drinking and low physical activity were also associated with low RBC folate concentrations in both male and female students. In order to improve folate nutritional status of college students, the practice of desirable health-related behaviors, such as non-smoking, moderate alcohol drinking, regular physical activity, and maintenance of healthy BMI should be encouraged along with consumption of folate-rich foods and supplements.

• 한국식품영양학회지
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• 제19권4호
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• pp.539-551
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• 2006

This study was to investigate the nutrient intakes from infant formula and supplemental foods of 129 infants(boys 69, girls 60) fed formula, aged from 5 to 12 months. Subjects were divided into $5{\sim}6\;months,\;7{\sim}8\;months,\;9{\sim}10\;months,\;and\;11{\sim}12\;months$ by ages. Dietary assessment was carried out by using 24-hour-recall method. The average intakes and feeding frequency of infant formula were $702m{\ell}$, 4.4 times in boys and $815m{\ell}$, 4.8 times in girls, respectively. Supplemental foods were introduced at the age of $4{\sim}6\;months$ in 86% of the infants. Foods introduced first as the supplemental food were rice gruel soup, commercial weaning foods, fruit juice. Energy intakes were similar to RDA. Daily intakes of calcium at all age groups were higher than the RDA, therefore, calciun overnutrition were elucidated. Average intakes of protein, phosphorus, iron, zinc, vitamin A, vitamin $B_1,\;vitamin\;B_2$, niacin, vitamin $B_6$, vitamin C were above RDA, folate did not meet RDA of infants at all ages. In conclusion, the average status of nutrient intakes of infants was fairly good, however, nutrient intakes from supplemental foods were lower in girls than in boys of $10{\sim}12$months, and folate status seemed to be poor. Therefore, mothers feeding infant formula to their infants should be educated for the importance of supplemental foods and its practice to support good nutrition.