• Applied Biological Chemistry
• /
• v.38 no.4
• /
• pp.293-296
• /
• 1995

Fig leaves were extracted with methanol and then fractionated with ethyl acetate and various buffers to get active fractions and determined the antimicrobial activities. The acidic and phenolic fractions fractionated from the methanol extract of fig leaves showed the strong antimicrobial activities, but the basic and neutral fractions did not show any activities. The degree of antimicrobial activities of phenolic fraction against tested bacteria was higher than those of acidic fraction, but these against yeasts and mold were almost equivalent to those of acidic fraction. Especially, phenolic fraction was mostly affected on Staphylococcus aureus and Pseudomonas aeruginosa. Four antimicrobial substances purified from the phenolic fraction which showed the strongest antimicrobial activities among the fractions from fig leaves, were identified as psoralen($C_{11}H_{6}O_{3}$, MW. 186), bergapten($C_{12}H_{8}O_{4}$, MW. 216), ${\beta}$-sitosterol($C_{29}H_{50}O$, MW. 414) and umbelliferone ($C_{9}H_{6}O_{3}$, MW. 162).

• Applied Biological Chemistry
• /
• v.38 no.4
• /
• pp.289-292
• /
• 1995

Fig leaves were extracted with methanol and then fractionated with ethyl acetate and various buffers to get active fractions, and their antimicrobial activities in each fraction were determined. Acidic fraction and phenolic fraction of fig leaves showed strong antimicrobial activities, but the basic fraction and neutral fraction did not show any activities. The degree of antimicrobial activities in phenolic fraction against tested bacteria were higher than those in acidic fraction, but these against yeasts and mold were almost equivalent to those in acidic fraction. Especially, phenolic fraction showed the strongest activities against Staphylococcuns aureus and Pseudomonas aeruginosa. Although there were some differences among microorganisms, minimum inhibition concentrations(MIC) in acidic and phenolic fractions against tested microorganisms were 0.05 to 0.1 g eq./ml and 0.01 to 0.25 g eq./ml, on the basis of fresh sample weight, respectively. Minimum lethal concentrations(MLC) in acidic and phenolic fractions were 0.25 to 2.00 g eq./ml and 0.05 to 1.50 g eq./ml, respectively. These supposed the antimicrobial activities in phenolic fraction were generally higher than those in acidic fraction and also phenolic fraction had lower MIC and MLC values than acidic fraction.

• 한국전기화학회:학술대회논문집
• /
• 2004.06a
• /
• pp.103-108
• /
• 2004

The present paper relates to an apparatus in which all carbonaceous material such as coal, oil, plastics and any substance having carbon atoms as part of its constituents are reformed(gasified) into syngas at temperature above $1,200^{\circ}C$(KR patent No.0391121, and PCT/KR2001/01717 and PCT/KR2004/001020). It comprises a single-stage reforming reactor without catalyst and a syngas burner as shown in Fig.2. syngas is combusted with $O_2$ gas in the syngas bunter to produce $M_2O$ and $CO_2$ gas with exothermic heat. Reaction products are introduced into the reforming reactor, reaction heat from syngas burner elevate the temperature of reactor above $1,200^{\circ}C$, and reaction products reduce carbonaceous material down to CO and $H_2$ gases. Reactants and heat necessary for the reaction are provided through the syngas burner only, Neither $O_2$ gas nor steam are injected into the reforming reactor. Reformer is made of ceramic inner lining and sst outer casing. Multiple syngas burners may be connected to the reforming reactor in order to increase the syngas output, and a portion of the product syngas is recycled into syngas burner. The present reformer as shown in Fig.2 is suitable to gasify carbonaceous wastes without secondary pollutants formed from oxidation. Further, it can be miniaturized to accompany a fuel cell system as shown in Fig.3 The output syngas may be used to drive a fuel cell and a portion of electrical power generated in a fuel cell is used to heat a compact reformer up to $1,200^{\circ}C$ so that gas/liquid fossil fuel can efficiently reformed into syngas. The fuel cell serves as syngas burner in Fig.2. The reformation reaction is sustained through recycling a portion of product syngas into a fuel cell and using a portion of electric power generated to heat the reformer for continuous operation. Such reforming reactor may be miniaturized into a size of PC, then you have a Personal Reformer(PR).

• Journal of the Korean Geographical Society
• /
• v.30 no.3
• /
• pp.242-254
• /
• 1995

The Hadano Basin is located at a distance of about 70kms and 60kms from Tokyo and Yokohama and lies in the south-west part of the Kanto region in Japan. The basin area, which correspoends to the catchment of the Kaname River, is about areal size of 60.7$\textrm{km}^2$ and extends about length of 8kms in E-W direction and about width of 5kms in N-S direction (Fig.1). The Hadano basin is filled with thick pile of the alluvum from deposits composed of volcanic materials, mostly came from the Hakone Volcano and overlain by Fuji Volcanic ashes. Fluvial deposits form the good aquifer, therefore water resources of Handano City has been largely depending upon the eroundwater. Urbanization and industrialization of the basin has been rapid in the last thirty years, after activation of "Factory Attraction Policy of Hadano City" in 1956. Growth in population and number of factory due to urbanization changed the land-use pattern of the basin rapidly and increased the water demands. Therefore, Hadano City exploited a new source of water supply, and have introduced the prefectureal waterworks since 1976. On the other hand, the rapid urbanization has brought about the pollution of streams in the basin by domestic sewage and industrial waste water. Diffusion rate of sewerage systems in Hadano City is 38% in 1993. In ordcr to examine the impact of anthropogenic factors on river environments, the author took up the change of land-use and diffusion area of sewerage as parameters, and performed field surveys on water discharge and quality. The survey has been made at upstream and downstream of the main stream regularly per month, to get informati ons about the variation of discharge and water quality aiong the stream and its diurnal fluctuation. Annual variation has been analyzed based the data from Hadano City Office. The results are summarized as follows. 1. Stream discharge has been increasing by urbanization (Fig.3). Water quality (C $l^{-10}$ , N $H^{+}$$_{ 4}$-N, BOD) has been improving gradually after the application of sewerage service, yet water pollution load at the lower station has increased than that at the upper one because of the larger anthropogenic discharge volumes (Fig.4). 2. Corrclation coefficient of discharges between upper and lower was 0.81-0.92. Pollutant loads of the R. Kamame after the confluence with R. Kuzuha grew up by 2.4-3.7 times as compared with its upper reaches, and it increased to 3.7-6.9 times after the confluence with the R. Muro (Fig.5). 3. The changes of water quality along the stream can be divided into two groups (Fig.6a). First: water quality of the R. Kaname and R. Shijuhachisse is becoming worse towards the lower reaches because the water from branches are polluted. Second: water quality are improved in the lower where spring and small branch streams supply clear water, for example R. Mizunashi, R. Muro and R. Kuzuha. 4. Measured discharge at the upper station in the R. Shijuhachisse is 0.153㎥/sec, and about 55% of this is recharged until it reaches to the lower point. The R. Mizunashi has a discharge of 1.155㎥/sec at the upper point, is recharged 0.24㎥/sec until the midstream and groundwater spring 0.2㎥/sec at the lower reaches. R. Kuzuha recharged all the mountain runoff (0.2㎥/sec) at the upper reaches. The R. Muro is supplied by many springs and the estimated discharge of spring was 0.47㎥/sec (Fig.6b). 5. Diurmal variations in discharge and water quality are influenced clearly by domestic and industrial waste waters (Fig.7, 8).ed clearly by domestic and industrial waste waters (Fig.7, 8).

• Proceedings of the Korean Vacuum Society Conference
• /
• 2013.08a
• /
• pp.88-89
• /
• 2013

A variety of influenza A viruses from animal hosts are continuously prevalent throughout the world which cause human epidemics resulting millions of human infections and enormous industrial and economic damages. Thus, early diagnosis of such pathogen is of paramount importance for biomedical examination and public healthcare screening. To approach this issue, here we propose a fully integrated Rotary genetic analysis system, called Rotary Genetic Analyzer, for on-site detection of influenza A viruses with high speed. The Rotary Genetic Analyzer is made up of four parts including a disposable microchip, a servo motor for precise and high rate spinning of the chip, thermal blocks for temperature control, and a miniaturized optical fluorescence detector as shown Fig. 1. A thermal block made from duralumin is integrated with a film heater at the bottom and a resistance temperature detector (RTD) in the middle. For the efficient performance of RT-PCR, three thermal blocks are placed on the Rotary stage and the temperature of each block is corresponded to the thermal cycling, namely $95^{\circ}C$ (denature), $58^{\circ}C$ (annealing), and $72^{\circ}C$ (extension). Rotary RT-PCR was performed to amplify the target gene which was monitored by an optical fluorescent detector above the extension block. A disposable microdevice (10 cm diameter) consists of a solid-phase extraction based sample pretreatment unit, bead chamber, and 4 ${\mu}L$ of the PCR chamber as shown Fig. 2. The microchip is fabricated using a patterned polycarbonate (PC) sheet with 1 mm thickness and a PC film with 130 ${\mu}m$ thickness, which layers are thermally bonded at $138^{\circ}C$ using acetone vapour. Silicatreated microglass beads with 150~212 ${\mu}L$ diameter are introduced into the sample pretreatment chambers and held in place by weir structure for construction of solid-phase extraction system. Fig. 3 shows strobed images of sequential loading of three samples. Three samples were loaded into the reservoir simultaneously (Fig. 3A), then the influenza A H3N2 viral RNA sample was loaded at 5000 RPM for 10 sec (Fig. 3B). Washing buffer was followed at 5000 RPM for 5 min (Fig. 3C), and angular frequency was decreased to 100 RPM for siphon priming of PCR cocktail to the channel as shown in Figure 3D. Finally the PCR cocktail was loaded to the bead chamber at 2000 RPM for 10 sec, and then RPM was increased up to 5000 RPM for 1 min to obtain the as much as PCR cocktail containing the RNA template (Fig. 3E). In this system, the wastes from RNA samples and washing buffer were transported to the waste chamber, which is fully filled to the chamber with precise optimization. Then, the PCR cocktail was able to transport to the PCR chamber. Fig. 3F shows the final image of the sample pretreatment. PCR cocktail containing RNA template is successfully isolated from waste. To detect the influenza A H3N2 virus, the purified RNA with PCR cocktail in the PCR chamber was amplified by using performed the RNA capture on the proposed microdevice. The fluorescence images were described in Figure 4A at the 0, 40 cycles. The fluorescence signal (40 cycle) was drastically increased confirming the influenza A H3N2 virus. The real-time profiles were successfully obtained using the optical fluorescence detector as shown in Figure 4B. The Rotary PCR and off-chip PCR were compared with same amount of influenza A H3N2 virus. The Ct value of Rotary PCR was smaller than the off-chip PCR without contamination. The whole process of the sample pretreatment and RT-PCR could be accomplished in 30 min on the fully integrated Rotary Genetic Analyzer system. We have demonstrated a fully integrated and portable Rotary Genetic Analyzer for detection of the gene expression of influenza A virus, which has 'Sample-in-answer-out' capability including sample pretreatment, rotary amplification, and optical detection. Target gene amplification was real-time monitored using the integrated Rotary Genetic Analyzer system.

• Nuclear Medicine and Molecular Imaging
• /
• v.42 no.1
• /
• pp.79-80
• /
• 2008

Including malignancy, various disease can show abnormal uptake in bone marrow. We report a case of non-specific inflammatory FDG uptake in bone marrow mimicking malignancy. A 35-year old woman with fever of unknown origin (FUO) underwent $^{18}F$-FDG PET/CT to find out fever $^{18}F$-FDG and unknown malignancy. $^{18}F$-FDG was injected and imaged 1hr after injection with Discovery ST (GE, USA), $^{18}F$-FDG PET/CT whole body image showed abnormal uptake in lower extremities (Fig. 1). MRI and biopsy was also done in the sites of abnormal uptake. PET and MRI suspect malignancy (Fig. 2, 3), but biopsy result was non-specific inflammatory process (Fig. 4). The patient was improved her clinical condition after antibiotics therapy.

• Food Science of Animal Resources
• /
• v.18 no.1
• /
• pp.19-26
• /
• 1998

In order to study the tenderizing effect of the proteolytic enzyme, ficin, from fig fruit (Ficus carica L), the enzyme was purified from fig latex by precipitation and chromatography. The ficin separated from Bongraesi showed single band on SDS-PAGE. However, the ficin from Masui showed tow bands. The specific activity of ficin purified from Bongraesi species was 2.8 unit/mg protein and that from Masui species was 6.5 unit / mg protein. The amounts of ficin purified from 50 mL of crude latex of Bongraesi and Masui were 1,760 mg and 657 mg, respectively. the water holding capacity of beef decreased to the large extent, when sugar Bongraesi latex and Masui latex were added. The hardness of beef showed decreasing tendency with the time, however, after 60 min, it decreased and thereafter increased a little after 120 min. the hardness of beef decreased sharply with addition of the latex of Bongraesi and Masui. The Masui has more tenderizing effect than the Bongraesi. When meat was mixed with tenderizing agent(ficin) and not heated, the change of color showed significant difference (p＜0.01). when meat was mixed with tenderizing agent(ficin) and heated, the toughness showed significant difference (p＜0.01) and the softness showed significant difference (p＜0.001).

• Journal of Chest Surgery
• /
• v.11 no.3
• /
• pp.246-252
• /
• 1978

A three year and seven month old girl with moderate depression deformity of the sternum associated with a huge well defined homogenous hazy mass density of the upper half of the right hemithorax on plain chest x-ray had developed, exertionaI dyspnea (Figs1, 2 and 3). Correction of the funnel chest was carried out with modified Ravitch procedure and resection of the intrathoracic cystic mass was performed through an anterolateral thoracotomy incision in one stage operation satisfactorily (Figs. 7 and 8). On exploration, the mass, $15{\times}12{\times}10$cm in size, was connected to the bronchus at 1cm a bove the carina by a stalk (Fig. 4). The outer surface showed abundant vasculature. The specimen was filled with mucoid material; the inner surface was much trabeculated. glistening and smooth (Fig. 5 and 6). yficroscopically, the cyst was lined with simple or pseudostratified ciliated columnar epithelium. The cystic wall was composed of loose fibrous connective tissue, muscle layers, cartilages with some lymphocytic infiltration (Fig. 9). Isolated cases of funnel chest deformity and congenital bronchogenic cystic disease are not uncommon; however, the assocbtion of the two conditions is yery rare. Therefore. report and review of the literature was done.

• Journal of Chest Surgery
• /
• v.13 no.2
• /
• pp.134-137
• /
• 1980

This represents a case report of the retained polyethylene catheter fragment in superior vena cava. A 39 year old male was admitted to this Korea University Hospital a short time after compression wound on abdomen with heavy cement material in emergency room, a polyethylene catheter was introduced into the right subclavian vein through a needle. But when the polyethylene catheter was attempted to withdraw the catheter was severed by the beveled tip of the needle. Later that day, chest X-ray disclosed the presence of the fragment extending from right subclavian vein to the superior vena cava. {Fig. 1 and Fig. 2]. Local exploration by way of an infraclavicular incision was unsuccessful in locating the catheter fragment. Another attempt was then made remove the catheter by means a biotome, which is originally a device for the biopsy of the myocardium, introduced through the right great saphenous vein. This procedure, though well tolerated by the patient, was in vain. After 11 days later, during that time he was taken a laparotomy with drain, another operation for removal of retained catheter fragment was performed through median sternotomy. After exposure of the right subclavian vein, innominate vein, and superior vena cava, an incision 1 cm in |length was made directly over the palpated catheter. The catheter immediately was picked upward and removed. The length of the catheter was approximately 8 cm. [Fig 3 ] There was no evidence of thromboembolism from the catheter or other complications. The patient made an uneventful recovery, and was discharged asymptomatic on the 9th postoperative day.

• Proceedings of the KSRS Conference
• /
• 2002.10a
• /
• pp.417-422
• /
• 2002

Kakegawa City is located at 138 deg. E and 34 deg. 45 min. N, in the west of Shizuoka Prefecture. Its population is approximately eighty thousands and its area is 185.79 square kilometers as shown in Fig.1. The city government has been developing GIS for the purpose of land management and tax base calculation and is one of the most advanced local governments in Shizuoka Prefecture. The city government has been developing the database of various kinds of physical and social environmental information, but the city government uses small parts of the database only fur land management and tax base calculation. The purpose of this report is to show the database of kakegawa City as shown in Table 1 and propose the effective usage of the database. The database of Kakegawa City as shown in Fig.2 and Fig.3 is imported to GIS in our laboratory and reconstructed as the database retrievable from each individual land. The authors developed the retrieval system of each individual land and combined it to the reconstructed database. If our GIS is open to the public, a lot of citizens will get useful information about their environments.