• Journal of Korean Academy of Fundamentals of Nursing
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• v.13 no.2
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• pp.275-284
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• 2006

Purpose: The purpose of this study was to analyze tile trends in physiological variables in CAT research in nursing in Korea. Method: Of studies published in Korea between January, 2000 and December, 2005, 227 studies were analyzed according to the criteria of type, theme, and patterns in physiological variables related to CAT. Results: There were 72 articles on CAT research in which physiological variables were examined. The most frequently researched type of CAT was massage and in particular, foot massage. The most frequently used physiological variables in CAT research were blood pressure, pulse, and body temperature. Patients with high blood pressure were the most frequent subjects for CAT research. As to the effect of physiological variable by CAT type, foot massage showed a decline in blood pressure in all six research studies involving patients with high blood pressure. Conclusion: There is a need to describe accurately the mechanism by which CAT affects physiological variables. There is also a need for repetitive analysis to verify the effect, and meta-analysis for the effect on physiological variables according to type of CAT.

• Korean Journal of Microbiology
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• v.32 no.2
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• pp.155-162
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• 1994

Cells of Acinetobacter sp. strain JC1 DSM 3803, an aerobic monoxide-oxidizing bacterium, growing on glucose exhibited high catalase activity at the mid-exponential growth phase. The enzyme activity decreased gradually after then until the early stationary phase, increased again at the mid-stationary phase, and then decreased again thereafter. Cells growing on glucose was found to contain three kinds of catalses. Cat1, Cat2 and Cat3. The activities of Cat1 and Cat3 did change significantly during growth, but that of Cat2 exhibited significant variation. Cat3 was found to present only in cells growing on glucose, but not in cells growing on carbon monoxide of methanol. The activities of call and Cat3 in cell-free extracts were stable upon treatment with ethanol and chloroform, but decreased to some extent when the enzymewere treated with 2mM $H_2O_2$ and/or 3-amino-1,2,4-triazole (AT). Cat2 was found to be extremely sensitive to the ethanol-chloroform and $H_2O_2$ treatments, but was insensitive to the AT treatment. Cat1 exhibited enzyme activity after incubation for 1 min at 80$^{\circ}C$. Cat2 and Cat3 did not show enzyme activity after incubation for 1 min at 60$^{\circ}C$ and 70$^{\circ}C$, respectively. Cat2 was found to have peroxidase activity. Cat3 was purified to homogenity in seven steps. The molecular weight of the native enzyme was estimated to be 150,000. Sodium dodecyl sulfate-gel electrophoresis revealed two identical subunits of molecular weight 65,000. The enzyme was found to show two $K_m$ values of 39 mM and 58mM. The optimal pH for the enzyme activity was 7.0, but the activities at pH 6.0, 8.0, and 9.0, were found to be comparable to that at the optimal pH. The optimal temperature for the enzyme activity was found to be 40$^{\circ}C$. The enzyme also exhibited strong activity at 20$^{\circ}C$, 30$^{\circ}C$, and 50$^{\circ}C$. The purified enzyme was not affected by the ethanol-chloroform treatment. The enzyme, howerver, showed less than 10% of the original activity when it was treated with 12 mN AT, 0.1 mM $NaN_3$ of 1mM KCN.

• Journal of Microbiology and Biotechnology
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• v.31 no.2
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• pp.259-271
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• 2021

Many bacteria metabolize aromatic compounds via catechol as a catabolic intermediate, and possess multiple genes or clusters encoding catechol-cleavage enzymes. The presence of multiple isozyme-encoding genes is a widespread phenomenon that seems to give the carrying strains a selective advantage in the natural environment over those with only a single copy. In the naphthalene-degrading strain Pseudomonas putida ND6, catechol can be converted into intermediates of the tricarboxylic acid cycle via either the ortho- or meta-cleavage pathways. In this study, we demonstrated that the catechol ortho-cleavage pathway genes (catBICIAI and catBIICIIAII) on the chromosome play an important role. The catI and catII operons are co-transcribed, whereas catAI and catAII are under independent transcriptional regulation. We examined the binding of regulatory proteins to promoters. In the presence of cis-cis-muconate, a well-studied inducer of the cat gene cluster, CatRI and CatRII occupy an additional downstream site, designated as the activation binding site. Notably, CatRI binds to both the catI and catII promoters with high affinity, while CatRII binds weakly. This is likely caused by a T to G mutation in the G/T-N11-A motif. Specifically, we found that CatRI and CatRII regulate catBICIAI and catBIICIIAII in a cooperative manner, which provides new insights into naphthalene degradation.

• Korean Journal of Microbiology
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• v.27 no.3
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• pp.194-200
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• 1989

The nucleotide sequence of inducible chloramphenicol acetyl-transferase(CAT) gene isolated from a small plasmid pSBK203 of Staphylococcus aureus was determined. The base sequence shows that structural gene of pSBK203-CAT encodes a protein of 213 amino acids and has a leader region which encodes a short polypeptide of 9 amino-acids in its upstream. vertical bar /sup 35/S vertical bar-Methionine labelled CAT gene product in minicell showed almost same mobility with pC194-CAT of which molecular weight is 24Kdal on polyacrylamide gel electrophoresis. Predicted amino acid sequence of pSBK203-CAT has revealed a high degree of homology with the CATs of pC194 and pC221 than those of cat-86, Tn9 and proteus mirabilis PM13.

• Korean Journal of Mathematics
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• v.27 no.2
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• pp.535-545
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• 2019

The main idea of this paper is to introduce the notion of $cat^1$-monoids and to prove that the category of crossed semimodules ${\mathcal{C}}=(A,B,{\partial})$ where A is a group is equivalent to the category of $cat^1$-monoids. This is a generalization of the well known equivalence between category of $cat^1$-groups and that of crossed modules over groups.

• Journal of Microbiology and Biotechnology
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• v.7 no.1
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• pp.21-24
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• 1997

Chloramphenicol acetyl transferase (CAT) protein and mRNA levels in E. coli were determined following induction of a tac::cat construct by isopropyl-${\beta}$-thiogalactopyranoside (IPTG). High cat mRNA levels did not directly reflect CAT protein levels, in either shakeflask experiments or fermentations. Furthermore, concentrations of IPTG resulting in the highest levels of expression of cat mRNA, were different to those resulting in highest levels of CAT protein. The data suggest that high transcriptional activities lead to limitations at the translational level.

• Journal of the Semiconductor & Display Technology
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• v.22 no.2
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• pp.11-16
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• 2023

EtherCAT is an Ethernet-based fieldbus system standardized in IEC 61158 and SEMI, and widely used in the fields of factory automation, semiconductor equipment and robotics. In this paper, without operating system, we have implemented an EtherCAT master with an open source EtherCAT master stack SOEM on STM32 Nucleo-144 board with an STM32F767 microcontroller. And its jitter performance has been evaluated at the output of the network port to include all the effects of the entire system in the results. The results show that the implemented EtherCAT master has precise control performance for control frequencies from 1KHz to 8KHz and relatively superior jitter performance compared to the EtherCAT masters with real-time patched Linux operating system.

• Korean Journal of Adult Nursing
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• v.14 no.1
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• pp.62-72
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• 2002

Purpose: This study was performed to develop a scale for evaluation of attitudes toward complementary and alternative therapies (CAT) and to investigate nurses' attitudes toward CAT. Method: The subjects were 263 nurses working at a university hospital in Seoul and Inchon. The personally designed questionnaire was tested for its reliability and validity. Nurses' attitudes to CAT were evaluated using the questionnaire. Results: Cronbach's $\alpha$ coefficient was 0.7405. 23 items were selected by item analysis and 4 factors including application, therapeutic effect, social interest and communication about CAT were classified by factor analysis. The mean score of attitudes and its subcategories were high, especially that of communication was very high. Nurses had a positive response to CAT in several items; acceptance as nursing intervention, its therapeutic value, complement for conventional medicine, and open communication about CAT. Attitudes were different significantly according to education and number of working years. There were high relationships between attitudes and its subcategories except communication. Conclusion: The Scale of attitudes toward CAT was proven to be reliable and valid. Positive nurses' attitudes toward CAT will help the patients be provided with a proper and safe way to take CAT.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.7C
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• pp.663-670
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• 2009

In this paper, we propose the image encryption method which gradually uses NFSR(Non-linear Feedback Shift Register) and 20 CAT(Two-Dimensional Cellular Automata Transform). The encryption method is processed in the following order. First, NFSR is used to create a PN(pseudo noise) sequence, which matches the size of the original image. Then, the created sequence goes through a XOR operation with the original image and process the encipherment. Next, the gateway value is set to produce a 20 CAT basis function. The produced basis function is multiplied by encryption image that has been converted to process the 20 CAT encipherment. Lastly, the results of the experiment which are key space analysis, entropy analysis, and sensitivity analysis verify that the proposed method is efficient and very secure.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2013.05a
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• pp.71-72
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• 2013