• Korean journal of applied entomology
• /
• v.56 no.1
• /
• pp.61-67
• /
• 2017

Two major sex pheromone components (Z-8-dodecenyl acetate and E-8-dodecenyl acetate) are known in the peach fruit moth, Grapholita molesta. From a putative biosynthetic pathway of these sex pheromone components, delta 10 desaturase ($10{\Delta}$ DES) has been proposed to play a crucial role in synthesizing a species-specific stereoisomer of the double bond. However, its molecular identity was not known. This study determined a putative desaturase (Gm-comp1575) as a $10{\Delta}$ DES candidate from G. molesta transcriptome constructed from the sex pheromone gland. Its open reading frame encodes 370 amino acid sequence with a predicted molecular weight at 43.2 kDa and isoelectric point at 8.77. It was predicted to have four transmembrane domains and six glycosylation sites at N-terminal or cytosolic domains. A phylogenetic analysis with its predicted amino acid sequence indicated that Gm-comp1575 is closely related with known $10{\Delta}$ DES genes of other insects. Gm-comp1575 transcript was detected in female adults at sex pheromone gland and other abdominal tissues. RNA interference of Gm-comp1575 significantly reduced attractiveness of virgin females in apple orchard compared to control females. These results suggest that Gm-comp1575 is associated with sex pheromone biosynthesis of G. molesta.

• Korean journal of applied entomology
• /
• v.43 no.3 s.136
• /
• pp.211-215
• /
• 2004

Temperature-fecundity of the melon aphid, Aphis gossypii Glover, was studied at constant temperatures ranging from 15 to $32.5^{\circ}C$ under $60-70\%$ RH and a photoperiod of 16 : 8 (L : D) A life table parameters were constructed using the results. The longevity of A. gossypii gradually increased with decreasing temperature below $27.5^{\circ}C$. Also fecundity increased with decreasing temperature and the highest fecundity was 61.8 nymphs per female at $17.5^{\circ}C$. However. daily fecundity increased with increasing temperatures up to $22.5^{\circ}C$ showing 5.9 nymphs per day and thereafter decreased. Longevity and fecundity of the adult in the greenhouse with an average temperature of $21^{\circ}C$ and $65.6\%$ RH, were 20.0 days and 59.6, respectively, which were longer and higher than those in the growth chamber with similar conditions. net reproductive rate (Ro) was 54.9 at $17.5^{\circ}C$ while intrinsic rate of increase ($r_m$) and finite rate of increase ($\lambda$) were the highest 0.5 and 1.6 at $30^{\circ}C$, respectively. doubling time (DT) and mean generation time (T) were the shortest 1.4 and 6.8 at $30^{\circ}C$ indicating that optimal temperature for the development is $30^{\circ}C$.

• Korean journal of applied entomology
• /
• v.34 no.4
• /
• pp.300-307
• /
• 1995

This study was carried out to investigate the life cycle of Cephonodes hylas (Linne), that is damaging to Gardenia jasminoides Ellis in the seedbed at Namhae during 1984 and 1985. Results are summarized as follows. The eggs were laid in mass on the leaf surface of the host plants. The oviposition period lasts for 26 days after late-May. The average number of eggs and average hatching rate were 20.36 and 97.61% , respectively. It takes 14-16 days until hatching. The egg shape was oval which its size was 1.01 mm in diameter and 1.48 mm in length. The body length of each larval instar from the 1st to the 5th were $3.52\pm$1.01, $6.46\pm$0.45, $13.19\pm$4.27, $24.08\pm$2.22 and $43.71\pm$5.62 mm, respectively. The average weight of each larval instar were $13.34\pm$2.41, $29.83\pm$6.88, $76.64\pm$31.31, $292.09\pm$107.70 and 2,$144.84\pm$760.70 mm, respectively. The larval period was observed from July to October, and total duration of larval stage was 116 days in which duration of each larval instar from the 1st to the 5th were 12, 36, 32, 16 and 20 days, respectively. During larval stage, a larvae feed on 17~18 seedlings. The 5th instar larvae built its place in soil for overwinter and became pupa. The body length of pupa was 33.6 mm and average weight was 2,032 mg Adult was emerged from overwintened pupa from mid through late-May in the followings year. The sex ratio of female was 42.82% and the color ratio of brown was 89.93%.

• The Journal of Korean Academy of Prosthodontics
• /
• v.39 no.6
• /
• pp.659-681
• /
• 2001

Platelet-rich plasma(PRP) has been known to increase the rate and degree of bone formation by virtue of growth factors in concentrated platelets. Although its great healing effect on bone defect or pre-implantation site preparation in conjunction with bone substitute has been reported, the effect associated with implant is unknown. The purpose of this study was to investigate the effect of PRP on rapid osseointegration of endosseous dental implants in the rabbit tibiae. Twenty two adult female New Zealand white rabbits, weighing approximately 2.7-3.3kg, were used for this study. Twelve of the 22 animals were used for histomorphometric analysis and ten of the 22 were for removal torque test. Each animal received two implants in each tibia (two treated with PRP and two as control) and was given fluorochrome intramuscularly. For histomorphometric analysis, rabbits were divided into four groups according to the healing period. At 1 week, 2 weeks, 4 weeks and 8 weeks postoperatively, each three animals were sacrificed serially and the amount and rate of bone formation around dental implant were examined on the undecalcified sections under fluorescent microscope, polarized microscope and light microscope connected to a personal computer equipped with image analysis system. For removal torque test, rabbits were divided into two groups and removal torque tests were performed at 4 weeks, 10 weeks after implant placement. In total, 88 screw shaped, commercially pure titanium implants (Neoplant, Neobiotech, Seoul, Korea) were used in this study. Labeling pattern reflected differences of two groups in bone formation rate at each period. Histomorphometrically, PRP group showed significantly higher bone volume within threads compared to control group at 2 weeks ($70.30{\pm}4.96%$ vs. $50.68{\pm}6.33%$; P < .01) and 4 weeks ($82.59{\pm}5.94%$ vs. $72.94{\pm}4.57%$; P < .05 ). PRP group at 1, 2 and 4 weeks revealed similar degree of bone volume formation comparable to control group at 2, 4 and 8 weeks, respectively. On the other hand, while PRP group showed higher bone-implant contact ($47.37{\pm}8.09%$) than control group ($33.16{\pm}13.47%$) at 2 weeks, there were no significant differences between PRP group and control group for any experimental period. Removal torque values also showed no significant differences between PRP group and control group at any experimental period (P > .05). These findings imply that PRP could induce rapid, more bone formation around implant during early healing period and get faster secondary stability for reducing healing period, though it has not induced bone maturation enough to resist functional loading.

• The Journal of Korean Physical Therapy
• /
• v.22 no.3
• /
• pp.99-105
• /
• 2010

Purpose: The purpose of this study was to evaluate the development of pain behavior and the expression of CCL2/CCR2 and CX3CL1/CX3CR1 at above and below the level of hemisection of the spinal cord in a rat model. Methods: Spinal cords of adult female Sprague-Dawley rats (n= 16, 200~250 g, 6~8 weeks old) were hemisected at T13 on the right side to develop the spinal hemisection injury model. We compared behavioral responses of the hemisection and of a sham surgery group. Behavioral tests for motor function (by the BBB locomotor scale), and for pain response for mechanical and cold allodynia were assessed postoperatively (PO) for 21 days. Expression of mRNA for chemokines and their receptors (CCL2/CCR2 and CX3CL1/CX3CR1) below and above the level of the spinal cord dissection were examined by RT-PCR. Results: We observed gradual motor improvement and the development of mechanical and cold allodynia on the ipsilateral hindpaw after spinal hemisection injury. We also found upregulation of mRNA expression of CCL2/CCR2 both above and below the level of spinal cord dissection but CX3CL1/CX3CR1 mRNA expression. Conclusion: Upregulation of CCL2/CCR2 is associated with neuropathic pain after spinal hemisection injury. CCL2/CCR2 may play an important role in the development of neuropathic pain after SCI as well as of peripheral neuropathic pain. These findings may improve understanding of the pathophysiological mechanism of neuropathic pain after SCI.

• Korean journal of applied entomology
• /
• v.34 no.3
• /
• pp.249-255
• /
• 1995

These experiments were conducted to investigate the life history and the seasonal fluctuation of population density of tea red spider mite, Tetranychus kanzawai Kishida and to observe its natural enemies in the southern area. Under field-cage conditions, tea red spider mite occurred 11~12 generations. The average periods from egg to adult emergence were 8.7~13.1 days in summer and 12.6~24.9 days in spring and autumn. The average longevities of adults were 15.5~21.9 days in summer and 25.1~31.6 days in spring and autumn. The average number of eggs laid per female were 45.1 in summer and 52.6 in spring and autumn. Tea red spider mite population showed two peaks during April-June, and appeared third or forth peak during October-November. The density of all the stages was the lowest in July through August. Tea red spider mites stayed at lower parts of tea plants in March but they moved to upper parts in April though June, and they again moved to lower parts after autumn. Tea red spider mite overwintered as all stages. Adults were the dominant overwintering stage during this stage. The surveyed natural enemies of the tea red spider mite were 7 species including Amblyseius longispinosus (Evans), A. eharai Amitei et Swirski, Anystis baccarun (L), Agistemus fleschneri Summers, Orius sauteri Poppius, Oligota yasumatsui Kistner and Scolothrips sp.

• Korean journal of applied entomology
• /
• v.32 no.3
• /
• pp.354-371
• /
• 1993

Behavior of adult females of the smoky brown cockroach was observed for 10-15 days continuously by using computer and the automatic sensoring system. Under the light condition of l2L~ 12D, individual variations were generally higher and the periodicity appeared less in terms of the presence time at the micro-habitats and the locomotory activity. The smoky brown cockroach appeared 20.42, 11.50, 6.31 and 2.66 % in a day in averages respectively at the shelter and the places for other individuals, feeding and drinking. It stayed 20.29 % in a day at the shelter when food, water, and other individuals were not supplied. Visiting rates were higher at the feeding and dnnking places than at. the ot.her micro-habitats. The degree of t.he locomotory activity was relatively lower when food, water, and other individuals were not supplied t.han when they were supplied. The Pnncipal Component Analysis (PCA) on the presence at the micro-habitats showed that. t.he presence pattern for each mdividual appeared differently (Q mode) while the differem time zones were grouped to the photophase and scotophase (R mode). When food, water, and other individuals were supplied the degree of grouping was higher at the shelter than at the places for feeding and drinking. When the act.ivityand the presence time at the different micro-habitats were jointly analyzed by PCA, the achvity of the female smoky brown cockroach appeared in relation with the presence time ( %) at the places for feeding and other individuals.

• Korean journal of applied entomology
• /
• v.53 no.4
• /
• pp.381-389
• /
• 2014

We investigated the host fruit, seasonal occurrence, and oviposition habits of the eurytomid wasp, Eurytoma maslovskii that cause Japanese apricot fruit drops. This eurytomid wasp was found to occur in Gyeonggi-do, Chungcheongbuk-do, Chungcheongnam-do, Jeollanam-do, and Jeollabuk-do in Korea, and it attacked seeds of Japanese apricot, apricot, and peach. Overwintered larvae were observed until mid April, inside the stone of Japanese apricots. Pupation took place between late March and late April. More than 90% of the adult wasps emerged between late April and early May. The female wasps laid eggs just under the seed coat before endocarp hardening. The length of the wasp ovipositor was at least 5 mm, and hence, the diameters of fruits for oviposition would not exceed 2 cm. We observed up to five eggs per fruit, and the egg stage did not last for more than two days. Newly hatched larvae moved to the embryo, which is the first part that forms in a seed, and feeds on this. Larval competition occurs during this time; in this study, only one larva survived and matured. Thus, from the larva cycle of the wasp, most of the damaged fruits would have fallen by early June, before the harvest period.

• Journal of Korean society of Dental Hygiene
• /
• v.12 no.3
• /
• pp.621-629
• /
• 2012

Objectives : The purpose of this study was to examine the influential factors for the oral-health practice of adult workers and the relationship of their health-promotion life style to the factors of oral-health practice in an attempt to determine the impact of health-promotion life style. Methods : The subjects in this study were 160 workers who worked in Seoul, North-Jeolla and South-Jeolla Province, and were at the age of 20 and up. The survey was conducted from February 1 to July 20, 2011. Results : As for the relationship of the general characteristics of the workers to the factors of their health-promotion practice, gender made statistically significant differences to toothbrushing female (3.24), and there were statistically significant differences in education/concern according to more than college(2.72) scored higher academic credential(p<0.05). The group of the workers whose health-promotion life style was better in terms of all the toothbrushing(3.35), use of oral hygiene supplies(2.19), regular dental-clinic visit(2.70), dietary control(2.84) and education/concern(3.20) scored higher, and there were statistically significant differences in the factors of education/concern(p<0.05). Their health-promotion life style had an impact on regular dental-clinic visit among the factors of oral-health practice(p<0.05), and there was a positive correlation between health-promotion life style and tooth brushing, dietary control(p<0.05), regular dental-clinic visit, education and concern(p<0.01) the factors of oral-health practice. Conclusions : Workers should be stimulated to get into sound life habits to change their behavior, and they should be urged to improve their preventive health care, to get a dental checkup and ultimately to promote their health as well. Companies should take measures to accelerate the oral health promotion of workers and provide more dental checkup programs for them to be more concerned about their oral health, to promote their oral health and to maintain the best oral health.

• Journal of Korean Biological Nursing Science
• /
• v.1 no.1
• /
• pp.71-85
• /
• 1999

The purpose of this study was to determine the effect of periodic low-intensity exercise during hindlimb suspension on the mass, relative weight, myofibrillar protein content in soleus, plantaris and gastrocnemius muscles. To examine the effectiveness of periodic low-intensity exercise on mass, and myofibrillar protein content of hindlimb muscles, adult female Wistar rats were suspended(HS) and half of these rats walked on a treadmill for 45min/day(15 min every 4h) at 5m/min and a $15^{\circ}$ grade(HS-EX). Soleus wet weight was 33.51% significantly smaller(p<0.005) and relative soleus weight of hindlimb suspended rats was 31.96% smaller(p<0.005) compared with those of control rats following seven days of hindlimb suspension. Plantaris wet weight was 7.5% smaller(p<0.01) and relative plantaris weight was 11.83% smaller(p<0.05) compared with those of control rats following seven days of hindlimb suspension. Gastrocnemius wet weight was 11.31% significantly smaller(p<0.005) and relative gastrocnemius weight was 17.13% significantly smaller(p<0.005) compared with those of control rats following seven days of hindlimb suspension. Soleus wet weight while increased by relative soleus weight increased by 25.13%, 27.59% each through periodic low intensity exercise during hindlimb suspension(p<0.05, p<0.05). Plantaris wet weight and relative plantaris weight increased by 1.04%, 10.98%(p<0.05) each, and gastrocnemius wet weight and relative gastrocnemius weight increased by 1.98%, 12.02%(p<0.05) each through periodic low intensity exercise during hindlimb suspension. Wet weight of soleus, plantaris and gastrocnemius in HS-EX rats did not recover to control level. Myofibrillar protein content of soleus, plantaris and gastrocnemius was 48.24%, 40.85% and 37.33% significantly smaller(p<0.005) respectively compared with those of control rats following seven days of hindlimb suspension. Myofibrillar protein content of soleus, plantaris and gastrocnemius increased by 40.68%, 25.07% and 17.93%(p<0.005) each through periodic low intensity exercise during hindlimb suspension. Myofibrillar protein content of soleus, plantaris, and gastrocnemius in HS-EX rats did not recover to control level. The results suggest that periodic low intensity exercise can attenuate hindlimb muscle atrophy induced by hindlimb suspension.