• The Korean Journal of Physiology and Pharmacology
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• v.10 no.6
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• pp.329-335
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• 2006

This study was aimed to investigate the nitric oxide (NO)-induced cytotoxic mechanism in PC12 cells. Sodium nitroprusside (SNP), an NO donor, decreased the viability of PC12 cells in dose-and time-dependent manners. SNP enhanced the production of reactive oxygen species (ROS), and gave rise to apoptotic morphological changes including cell shrinkage, chromatin condensation, and DNA fragmentation. Expression of Bax was not affected, whereas Bcl-2 was downregulated in SNP-treated PC12 cells. SNP augmented the release of cytochrome c from mitochondria into cytosol and enhanced caspase -8, -9, and -3 activities. SNP upregulated both Fas and Fas-L, which are known to be components of death receptor assembly. These results suggest that NO induces apoptosis of PC12 cells through both mitochondria-and death receptor-mediated pathways mediated by ROS and Bcl-2 family.

• Journal of Plant Biotechnology
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• v.43 no.2
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• pp.181-188
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• 2016

In this study, we developed 15 novel polymorphic simple sequence repeat (SSR) markers by SSR-enriched genomic library construction from Codonopsis lanceolata. We obtained a total of 226 non-redundant contig sequences from the assembly process and designed primer sets. These markers were applied to 53 accessions representing the cultivated C. lanceolata in South Korea. Fifteen markers were sufficiently polymorphic, and were used to analyze the genetic relationships between the cultivated C. lanceolata. One hundred three alleles of the 15 SSR markers ranged from 3 to 19 alleles at each locus, with an average of 6.87. By cluster analysis, we detected clear genetic differences in most of the accessions, with genetic distance varying from 0.73 to 0.93. Phylogenic analysis indicated that the accessions that were collected from the same area were distributed evenly in the phylogenetic tree. These results indicate that there is no correlative genetic relationship between geographic areas. These markers will be useful in differentiating C. lanceolata genetic resources and in selecting suitable lines for a systemic breeding program.

• Perspectives in Nursing Science
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• v.16 no.1
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• pp.45-54
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• 2019

Purpose: The purposes of this study was to review the literature on intervention for treating anxiety and depression among unmarried mothers living in facilities, and to understand core that could promote the development of more effective interventions. Methods: Key words in English and Korean were used to search through eight electronic databases-PubMed, Cochrane Library, EMBASE, CINAHL, RISS, DBpia, NDSL, and the National Assembly Library. Results: Ten studies were ultimately selected for the integrative review and were evaluated in terms of contextual and methodological quality. The studies consisted of seven quasi-experimental studies and three case report studies. The selected studies utilized music, art, forest therapy, dancing, education, and play programs to change mothers' perceptions, emotions, and behavior and to improve their relationships with their babies or others. Conclusion: It is important to consider mothers' self-awareness and emotional expression, and to improve their relationships with their babies or others as core elements when developing intervention programs for anxiety and/or depression among unmarried mothers living in residential facilities.

• The Korean Journal of Archival Studies
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• no.41
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• pp.39-73
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• 2014

This study aims to analyze the features and problems of the Korea's national records archiving system established in 1999, as well as to suggest the countermeasures. First, in Korea, permanent records are archived by the administration, legislature and judicature separately. However, around the world there are very few cases where a national archives controls the records of the administration alone, whereupon there is the need to set up an integrated archiving system regarding the records of the administration, legislature and judicature. Second, in Korea, the records archiving system leans too much towards the centrally-controlled system, which is urgent to be solved. 'Record Management Act' stipulates that all the public records, including nonpermanent ones, shall be managed by National Archives, wherewith National Archives is overburdened. Hereat, it may be desirable for National Archives to manage very important records that deserve to be archived permanently and for Record Center to archive other records, which needs to be made into law.

• International Journal of Stem Cells
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• v.15 no.1
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• pp.85-94
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• 2022

Background and Objectives: Brain organoids have the potential to improve our understanding of brain development and neurological disease. Despite the importance of brain organoids, the effect of vascularization on brain organoids is largely unknown. The objective of this study is to develop vascularized organoids by assembling vascular spheroids with cerebral organoids. Methods and Results: In this study, vascularized spheroids were generated from non-adherent microwell culture system of human umbilical vein endothelial cells, human dermal fibroblasts and human umbilical cord blood derived mesenchymal stem cells. These vascular spheroids were used for fusion with iPSCs induced cerebral organoids. Immunostaining studies of vascularized organoids demonstrated well organized vascular structures and reduced apoptosis. We showed that the vascularization in cerebral organoids up-regulated the Wnt/β-catenin signaling. Conclusions: We developed vascularized cerebral organoids through assembly of brain organoids with vascular spheroids. This method could not only provide a model to study human cortical development but also represent an opportunity to explore neurological disease.

• Journal of Microbiology and Biotechnology
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• v.27 no.8
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• pp.1419-1427
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• 2017

As probiotics play an important role in maintaining a healthy gut flora environment through antitoxin activity and inhibition of pathogen colonization, they have been of interest to the medical research community for quite some time now. Probiotic bacteria such as Lactobacillus plantarum, which can be found in fermented food, are of particular interest given their easy accessibility. We performed whole-genome sequencing and genomic analysis on a GB-LP1 strain of L. plantarum isolated from Korean traditional fermented food; this strain is well known for its functions in immune response, suppression of pathogen growth, and antitoxin effects. The complete genome sequence of GB-LP1 is a single chromosome of 3,040,388 bp with 2,899 predicted open reading frames. Genomic analysis of GB-LP1 revealed two CRISPR regions and genes showing accelerated evolution, which may have antibiotic and antitoxin functions. The aim of the present study was to predict strain specific-genomic characteristics and assess the potential of this new strain as lactic acid bacteria at the genomic level using in silico analysis. These results provide insight into the L. plantarum species as well as confirm the possibility of its utility as a candidate probiotic.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.34 no.1
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• pp.44-55
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• 2021

Objectives : The aim of this article is to compare the effect of the korean medicine alone with korean-western combination treatment to herpes zoster. Methods : Domestic databases(OASIS, NDSL, RISS, KISS, KTKP, KMBASE, DBPIA, KoreaMed, National Assembly Library) were used to search case study papers related to herpes zoster and korean medicine treatment. We searched them using the term 'Herpes zoster' or 'Ramsey Hunt' combinated with 'Acupuntcure', 'Herbal medicine' or 'Korean medicine'. Results : A total of 36 studies were selected and analyzed. 25 studies treated with korean medicine and 11 studies treated with korean-western combination treatment which include any western medication. Of these 36 papers, there were a total of 24 cases in which a VAS test was undertaken. According to 36 published papers, no statistically significant difference in the periods of treatment with korean medicine alone from those of korean-western combination treatment was found. Also, no statistical difference in the VAS changes per period with korean medicine alone from those with korean-western combination treatment was found. Conclusions : According to the results, These findings suggest that korean medicine treatment can be effective for the herpes zoster the same as korean-western combination treatment.

• Fisheries and Aquatic Sciences
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• v.10 no.3
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• pp.119-126
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• 2007

The analysis of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and the development of resources for functional genomics. To analyze the transcriptome of the abalone Haliotis discus hannai, we conducted EST analysis using seven cDNA libraries made from gill, gut, hepatopancreas, skin, muscle, testis, and ovary. Redundant ESTs were assembled into overlapping contiguous sequences using the assembly program ICAtools. We found that the total 1,393 ESTs formed 135 clusters and 951 singletons, indicating that the overall redundancy of the library was 22%. Of the 1,393 clones, BLAST identified 1,278 clones (91.7%) as known genes; 115 clones (8.3%) did not match any previously described gene. Based on the major functions of their encoded proteins, the identified clones were classified into 16 broad categories. Sequence analysis revealed the presence of micro satellite-containing genes that may be valuable for further gene mapping studies. This study contributes to the identification of numerous EST clones that can be applied to further clarifying the genetics and developmental biology of abalone.

• The Korean Journal of Archival Studies
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• no.14
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• pp.289-323
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• 2006

The first organization of the 'national Archives of France' was defined by a law voted during the Revolution, the law of June 25, 1794 which is remained into force during nearly two century-until with the law of January 3, 1979. This law is regarded as the text founder of the national Archives of France, mainly thanks to its article 48 which posed the principle of the free communicability of the whole of the documents of national Archives of France. But it had initially as an aim to organize and frame sortings of the documents gathered in great number since the beginning of the French revolution. Its principal provision does not leave astonish us: it envisaged a separation between the documents to be eliminated, the useful documents has the administration, only intended to be preserved in national Archives of France?, and the documents interesting for the history, which were intended for the national Library.

• The Korean Journal of Archival Studies
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• no.40
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• pp.3-27
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• 2014

The purpose of the enactment of the 'Law on the National Archives of Korea' should be to secure the accountability and to shape up the improved governance system in the field of records and archival management, which will contribute to the strengthening of democracy in records management and eventually to the widening of the democratic progress in Korea. On the basis of the above recognition and by way of notion called 'horizontal accountability', this paper suggests the way to secure the National Archives of Korea's independency. And the way to reorganize the National Records Management Committee based on the pluralism and the 'multi-layered' governance system is also investigated. One of the main directions in the suggested law should be for the autonomy and transparency in the operation of the National Archives of Korea. This paper tries drawbacks and limitations of the 'Bill on the National Archives of Korea' introduced to the National Assembly last year, and suggests the alternatives as wel lto get further forward with related discussion.