• Journal of fish pathology
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• v.33 no.2
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• pp.103-109
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• 2020

In order to use nervous necrosis virus (NNV) virus-like particles (VLPs) as a delivery tool for heterologous antigens or plasmids, we attempted to produce red-spotted grouper nervous necrosis virus (RGNNV) VLPs displaying a partial region of viral hemorrhagic septicemia virus (VHSV) glycoprotein at the surface and VLPs that are harboring DNA vaccine plasmids within the VLP. A peptide encoding 105 amino acids of VHSV glycoprotein was genetically inserted in the loop region of NNV capsid gene, and VLPs expressing the partial part of VHSV glycoprotein were successfully produced. However, in the transmission electron microscope analysis, the shape and size of the partial VHSV glycoprotein-expressing NNV VLPs were irregular and variable, respectively, indicating that the normal assembly of capsid proteins was inhibited by the relatively long foreign peptide (105 aa) on the loop region. To encapsulate by simultaneous transformation with both NNV capsid gene expressing plasmids and DNA vaccine plasmids (having an eGFP expressing cassette under the CMV promoter), NNV VLPs containing plasmids were produced. The encapsulation of plasmids in the NNV VLPs was demonstrated by PCR and cells exposed to the VLPs encapsulating DNA vaccine plasmids showed fluorescence. These results suggest that the encapsulation of plasmids in NNV VLPs can be done with a simple one-step process, excluding the process of disassembly-reassembly of VLPs, and NNV VLPs can be used as a delivery tool for DNA vaccine vectors.

• Journal of Electrochemical Science and Technology
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• v.13 no.1
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• pp.159-166
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• 2022

The assembly of the micron-sized Si/CNT/carbon composite wrapped with graphene (SCG composite) is designed and synthesized via a spray drying process. The spherical SCG composite exhibits a high discharge capacity of 1789 mAh g^-1 with an initial coulombic efficiency of 84 %. Moreover, the porous architecture of SCG composite is beneficial for enhancing cycling stability and rate capability. In practice, a blended electrode consisting of spherical SCG composite and natural graphite with a reversible capacity of ~500 mAh g^-1, shows a stable cycle performance with high cycling efficiencies (> 99.5%) during 100 cycles. These superior electrochemical performance are mainly attributed to the robust design and structural stability of the SCG composite during charge and discharge process. It appears that despite the fracture of micro-sized Si particles during repeated cycling, the electrical contact of Si particles can be maintained within the SCG composite by suppressing the direct contact of Si particles with electrolytes.

• Journal of the Microelectronics and Packaging Society
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• v.28 no.4
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• pp.47-50
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• 2021

Nanoimprint lithography (NIL) has revolutionized the fabrications of electronics, photonics, optical and biological devices. Among all the NIL processes, roll-to-roll nanoimprinting is regarded best for having the attributes of low cost, continuous, simple, and energy-efficient process for nanoscale device fabrication. However, large-area printing is limited by the master mold deformation. In this study, a finite element model (FEM) has been constructed to assess the deformation of the roll mold adhesively wrapped on the carbon fiber reinforced material (CFRP) base roll. This study also optimizes the deformations in the metallic roll mold with respect to nip-forces applied in the printing process of nano-fabrication on large scale. The numerical simulations were also conducted to evaluate the deflection in roll mold assembly due to gravity. The results have shown decreasing trend of the deformation with decreasing nip-force. Also, pressure uniformity of about 40% has been optimized by using the current numerical model along with an acceptable deflection value in the vertical axis due to gravity.

• International Journal of Advanced Culture Technology
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• v.11 no.3
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• pp.51-58
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• 2023

Through this incident, it reminds me of the idea that personnel appointments are everything once again. Public officials should try to be good at ethics and conduct. If a public official fails to fulfill his or her role as a public official, how can the public trust him or her? You should know that the country does not exist under that official. Looking back on the constitutional history after liberation, it is undeniable that the collusion between politicians and businessmen shocked the people with many suspicions and distrust, and the various irregularities they caused disrupted healthy democracy and the national economic order. In this respect, this paper focuses on Korean political reform measures, but also examines ways to improve public officials' ethics. This paper focuses on that political reform should move in the direction of revitalizing the function of the National Assembly and allowing autonomous and independent parliamentary activities. Parliamentary activities should not be too dampened, and it should not end in a brief promotion in consideration of election votes. The ruling and opposition parties should seriously consider and implement this political reform plan. As one of the people, I hope that this privilege reform plan can be a touchstone for the advancement of political culture. This paper was studied as follows. First, the purpose and justification of political reform were identified. Second, it emphasized the need to improve public service ethics. Third, the problems and improvement measures of political reform were considered.

• Journal of Korean Medical classics
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• v.23 no.4
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• pp.39-62
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• 2010

Background : I investigated clinical research methodology of Traditional Korean Medicine(TKM) for treatment of lung cancer with Evidence Based Medicine(EBM) approach. Methods : I conducted the internet search of National Central Library, the National Assembly Library, Korea Education & Research Information Service, Korea Institute of Science and Technology Information for Korean theses and papers, and MEDLINE for the outside articles. Conclusion : In addition to outcomes of clinical trial, abundant clinician's experience and patients' preference are also considered. Pragmatic trials and quasi-experimental trials are better than clinical trials to plan clinical design of TKM for lung cancer and appropriate endpoints includes overall survival, disease-free survival, patient reporting outcome.

• Journal of Dental Rehabilitation and Applied Science
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• v.28 no.2
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• pp.147-161
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• 2012

This study is to assess the effect of horizontal misfit of an implant supported 3-unit fixed prosthodontics on the stress development at the marginal cortical bone surrounding implant neck. Two finite element models consisting of a three unit fixed prosthodontics and an implant/bone complex were constructed on a three dimensional basis. The three unit fixed prosthodontics were designed either shorter (d=17.8mm model) or longer (d=18.0mm model) by 0.1mm than the span of two implants placed at the mandibular second premolar and second molar areas 17.9mm apart. Fitting of the fixed prosthodontics onto the implant abutments was simulated by a total of 6 steps, that is to say, 0.1mm displacement per each step, using DEFORM 3D (ver 6.1, SFTC, Columbus, OH, USA) program. Stresses in the fixed prosthodontics and implants were evaluated using von-Mises stress, maximum compressive stress, and radial stress as necessary. The d=17.8mm model assembled successfully on to the implant abutments while d=18.0mm model did not. Regardless if the fixed prosthodontics fitted onto the abutments or not, excessively higher stresses developed during the course of assembly trial and thereafter. On the marginal cortical bone around implants during the assembly, the peak tensile and compressive stresses were as high as 186.9MPa and 114.1MPa, respectively, even after the final sitting of the fixed prosthodontics (for d=17.8mm model). For this case, the area of marginal bone subject to compressive stresses above 55MPa, equivalent of the $4,000{\mu}{\varepsilon}$, i.e. the reported threshold strain to inhibit physiological remodeling of human cortical bone, extended up to 2mm away from implant during the assembly. Horizontal misfit of 0.1mm can produce excessively high stresses on the marginal cortical bone not only during the fixed prosthodontics assembly but also thereafter.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.8 no.1
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• pp.1-23
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• 1998

Work-related Low Back Pain(LBP) is one of tile most important Issues in the field of industrial safety and health. Particularly, manual lifting is known as a major cause of work-related LBP and impairment. Total number of 163 manual lifting tasks in motor assembly processes were investigated. The 1981 and the 1994 equations developed by National Institute for Occupational Safety and Health(NIOSH) were applied to evaluate potential hazards of lifting-related LBP. Comparisons between the 19R I and 1994 NIOSH criteria were made. The relationships between the NIOSH criteria and lifting-related LBP were also analyzed. The results of this study are as follows: 1. The values of Action Limit(AL) by the NIOSH 1981 lifting equation. Recommended Weight Limit(RWL) by the 1994 equation and the weight of the load handled at each manual lifting task were shown log-normal distributions. 2. LI'(the weight of tile load/AL) and LI(the weight of the load/RWL) were calculated estimate the physical stress imposed by each individual lifting task. As a result. 76.7% of the total LI' value exceeded 1 and 12.9% exceeded 3, and 84.7% of the total LI values exceeded 1 and 20.9% exceeded 3. 3. Bus 2 Department showed the highest rate of LI'>1 and LI>1 and Bus 1 Department showed the highest rate of LI'>3 and LI>3 4. In general, the RWLs by the 1994 equation were found lower than the ALs by the 1981 equation. It is assumed to he resulted from the fact that the 1994 equation includes methods evaluating asymmetrical lifting tasks and lifts of objects with less then optimal hand-container couplings, and also covers a larger range of work durations and lifting frequencies than the 1981 equation. 5. Significant correlations were found between LI' and incidence of LBP (R=0.734, p<0.05). LI and incidence of LBP(R=0.671. p<0.10) and load-weights and incidence of LBP(R=0.797, p<0.05). 6. Control measures are required to achieve the value of LI less than 1 for some tasks having high LI values. Engineering control is highly recommended for some tasks having the value of LI above 3.

• Journal of the Korean Wood Science and Technology
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• v.42 no.6
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• pp.708-713
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• 2014

Trusses are widely used for wooden houses in the areas where wood construction in generalized for residential housings such as North America, Australian, New Zealand. In Korea, joist and rafter system is generally used because of the production cost, transportation cost and lack of experience required for truss manufacturing. In this study, roof trusses and flat trusses were manufactured by using oriented strand board (OSB) gusset plates for field assembly and tested under bending load to obtain the allowable loads. The allowable load and the actual load of 6m span roof trusses were 10.60 kN and 5.26 kN, respectively, which is regarded to be sufficient for use in construction. The allowable load and the actual load of 6m span floor flat trusses were 7.18 kN and 7.43 kN, respectively. For flat trusses, the allowable load is slightly lower than the actual load but the difference in very small, and it is thought that flat trusses can be used for construction by applying small change of structures and members.

• Journal of Physiology & Pathology in Korean Medicine
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• v.26 no.5
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• pp.599-609
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• 2012

The purpose of this study is to review the trend of Dong-gi acupuncture treatment and to suggest the future direction of its study. We performed literature search using KISS, RISS, NDSL, DBpia, OASIS, National Assembly Library and CNKI. We categorized literatures by year, diseases or symptoms, type of articles, motion types, acupoints, and clinical methods. There were few Korean researches found, but recently research in China has been growing rapidly. According to the researches, Dong-gi acupuncture treatment has been mainly used for painful symptoms of musculoskeletal diseases using distal acupoints. However, its field of application has been widening. It has been shown to be effective for a variety of diseases like cerebrovascular disease or neurologic disorder. We expect Dong-gi acupuncture treatment to be widely applied for various diseases and more clinical studies performed to prove the curative effect on various diseases.

• Journal of Agricultural Extension & Community Development
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• v.14 no.1
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• pp.29-57
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• 2007

Korea and USA reach an agreement with FTA. Korea's National Assembly will be to ratify the FTA. Korean agriculture was insufficiently competitive. FTA will be inflicted severe losses to Korean agriculture. But taking for granted that neoliberalism and economic globalization as the irrevocable currency of the international economic order, it is time to devote ourselves into discussing appropriate responses policy to minimize domestic agricultural loss and to improve agricultural competitiveness. There will never be another opportunity like this. It's now or never. Korean government and farmer have done various things, but basically their effort have to concentrate on three forms: fill the needs of an old market, create a need and a new export market, and adapt to a new market. This paper was an exploratory study of Korea－USA. FTA and paving the way for further studies on FTA in Korean agriculture. The objectives of this study Korea and USA reach an agreement with FTA. Korea’s National Assembly will be to ratify the FTA. Korean agriculture was insufficiently competitive. FTA will be inflicted severe losses to Korean agriculture. But taking for granted that neoliberalism and economic globalization as the irrevocable currency of the international economic order, it is time to devote ourselves into discussing appropriate responses policy to minimize domestic agricultural loss and to improve agricultural competitiveness. There will never be another opportunity like this. It's now or never. Korean government and farmer have done various things, but basically their effort have to concentrate on three forms: fill the needs of an old market, create a need and a new export market, and adapt to a new market. This paper was an exploratory study of Korea－USA. FTA and paving the way for further studies on FTA in Korean agriculture. The objectives of this study were 1)to explore the agricultural policy of Korean government, 2)and to explore the present condition of Korean agriculture, 3)and looking for responses direction of Korean agricultural policy to FTA, 4)to strengthen the competitiveness of Korean agriculture. Korean government give a subsidy not to strengthen the competitiveness but to soothe the dissatisfaction. It is necessary for Korean government to switch from public money investment to strengthen the competitiveness.