• Journal of Microbiology and Biotechnology
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• v.15 no.4
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• pp.767-771
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• 2005

Expression in yeast may prove more amenable to generating large amounts of viral antigens for a vaccine candidate. We, therefore, cloned the gene encoding the Hepatitis C virus (HCV) structural proteins (C-El-E2, c740) fused in-frame with, and immediately 3' to, the chicken-lysozyme signal peptide (C-SIG) gene and under the control of the yeast glyceraldehyde-3-phosphate dehydrogenase gene promoter. In yeast, the HCV structural proteins were expressed in two different forms: a processed and a nonprocessed aggregated form. Biophysical characterization by sucrose linear gradient centrifugation revealed that both forms were present in the same fractions with a buoyant density of 1.127-1.176 g/$cm^3$. These findings suggest that the efficient synthesis of HCV structural proteins in yeast may be an important tool to study virus assembly and may lead to the development of an HCV vaccine.

• Design & Manufacturing
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• v.11 no.1
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• pp.26-29
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• 2017

Burrs generated during shear forming such as notching and piercing may cause lifting during product assembly, which may deteriorate the productivity and quality of products. In this study, various shear angles and variable clearances between the punch and the die were applied in experimental notching tests to investigate the shear fracture surface and the burr height due to various conditions. The experimental results show that the clearance has the greatest effect on shear and fracture surfaces. It is considered that the height of the shear section increases slightly as the shear angle increases.

• Carbon letters
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• v.15 no.2
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• pp.136-141
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• 2014

Three dimensional self-assembled graphene hydrogels were easily fabricated by electron beam irradiation (EBI) using an aqueous solution of wool/poly(vinyl alcohol) and graphene oxide (GO). After exposure to various levels of EBI radiation, the highly porous, self-assembled, wool-based graphene hydrogels were characterized using scanning electron microscopy and Fourier-transform infrared spectroscopy; to determine the gel fraction, degree of swelling, gel strength, kinetics-of-swelling analyses and removal of hexavalent chromium (Cr(VI)) from the aqueous solution. X-ray diffraction results confirmed that EBI played a significantly important role in reducing GO to graphene. The adsorption equilibrium of Cr(VI) was reached within 80 min and the adsorption capacity was dramatically increased as the acidity of the initial solution was decreased from pH 5 to 2. Changes in ionic strength did not exert much effect on the adsorption behavior.

• BMB Reports
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• v.50 no.5
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• pp.247-256
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• 2017

CRISPR/Cas9 is the latest tool introduced in the field of genome engineering and is so far the best genome-editing tool as compared to its precedents such as, meganucleases, zinc finger nucleases (ZFNs) and transcription activator-like effectors (TALENs). The simple design and assembly of the CRISPR/Cas9 system makes genome editing easy to perform as it uses small guide RNAs that correspond to their DNA targets for high efficiency editing. This has helped open the doors for multiplexible genome targeting in many species that were intractable using old genetic perturbation techniques. Currently, The CRISPR system is revolutionizing the way biological researches are conducted and paves a bright future not only in research but also in medicine and biotechnology. In this review, we evaluated the history, types and structure, the mechanism of action of CRISPR/Cas System. In particular, we focused on the application of this powerful tool in autophagy research.

• Nuclear Engineering and Technology
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• v.55 no.6
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• pp.2195-2205
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• 2023

One of the salient nuclear fuel performance parameters for new fuel types under development is changes in fuel thickness. To test the new commercially fabricated U-10Mo monolithic plate-type fuel, an irradiation experiment was designed that consisted of multiple mini-plate capsules distributed within the Advanced Test Reactor (ATR) core, the mini-plate 1 (MP-1) experiment. Each capsule contains eight mini-plates that were either fueled or "dummy" plates. Fuel thickness changes within a fuel assembly can be characterized by measuring the gaps between the plates ultrasonically. The channel gap probe (CGP) system is designed to measure the gaps between the plates and will provide information that supports qualification of U-10Mo monolithic fuel. This study will discuss the design and the results from the use of a custom-designed CGP system for characterizing the gaps between mini-plates within the MP-1 capsules. To ensure accurate and repeatable data, acceptance and calibration procedures have been developed. Unfortunately, there is no "gold" standard measurement to compare to CGP measurements. An effort was made to use plate thickness obtained from post-irradiation measurements to derive channel gap estimates for comparison with the CGP characterization.

• Nuclear Engineering and Technology
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• v.55 no.7
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• pp.2591-2603
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• 2023

Simulating the Corrosion-Related Unidentified Deposit (CRUD) on the surface of fuel assemblies is necessary to predict the axial offset anomaly and the localized corrosion induced by the CRUD during the operation of nuclear power plants. A new CRUD model was developed to predict the formation of the CRUD deposits, considering the deposition and erosion mechanisms. The heat transfer and capillary flow within the CRUD were also considered to evaluate the boiling amount within the CRUD layer. This model predicted a CRUD deposit thickness of 44 ㎛ during a one-cycle operation of the Seabrook nuclear power plant. The CRUD deposition tended to accelerate and decelerate during the simulation, by being related to boiling mechanism on the deposits surface. Additionally, during a three-cycle operation corresponding to the refueling period, the CRUD deposition was saturated at a thickness of 80 ㎛, which was in good agreement with the suggested thickness for CRUD buildupin pressurized water reactors. Surface boiling on the thin CRUD deposits enhanced the acceleration of the deposition, even when the wick boiling properties were not favorable for CRUD deposition. To ensure the certainty of the simulation results, sensitivity analyses were conducted for the porosity, chimney density, and the constants employed in the proposed model of the CRUD.

• Journal of Animal Science and Technology
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• v.66 no.5
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• pp.1079-1082
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• 2024

Treponema pedis, a fastidious anaerobic spirochete, is one of the main pathogens involved in the development and progression of bovine digital dermatitis (BDD), a lameness-causing hoof infection in cattle. Here, the complete genome sequencing of T. pedis GNW45 isolated from a dairy cow infected with BDD, was presented. Libraries for long and short reads were sequenced using PacBioRSII and Illimuna HiSeqXTen platforms, respectively. De-novo assembly was done using the long reads, producing a circular contig, by which the short reads were aligned to generate a more accurate genome sequence. The genome has a total size of 3,077,465 base pairs, with 36.84% guanine-cytosine content. A total of 2,749 protein-coding sequences, seven ribosomal RNA's, and 45 transfer RNA's were annotated. Functional analysis revealed genes associated with pathogenicity and survivability in the complex pathobiome of BDD. This study provided novel insights into the survival and pathogenic mechanisms of T. pedis GNW45.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.45.3-46
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• 2015

We present a spectroscopic study of globular clusters in the M81 group that is one of the ideal laboratories for understanding mass assembly and evolution of galaxies, such as M81, the twin galaxy of the Milky Way, and the starburst galaxy M82, in the group environments. Spectra of about 800 globular cluster candidates are obtained using MMT/Hectospec, and about one hundred globular clusters are confirmed by their radial velocities. Based on the kinematics derived from the spectra, we have found that most globular clusters rotate around M81. We have also discovered more than ten globular clusters belonging to M82, and that their kinematics is different from that of young star clusters in the disk of M82. There are few candidates of intra-group globular clusters. We will discuss the implications of these results.

• Journal of the Korean Society for Precision Engineering
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• v.18 no.4
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• pp.129-134
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• 2001

It is essential to predict the welding deformation at assembly stage, to increase productivity through mechanization and automation effectively. A practical analysis method appled for production engineering was proposed to simulate the deformation of arc welding, with an analytical model using finite element method solving thermal-elastic-plastic behavior. In this research, for accurate assembling, 3-D thermal-elastic-plastic finite element model is used to simulate the out-of-plane deformation caused by arc welding. Efforts have been made to find out the efficient method to improve the reliability and accuracy of the numerical calculation. Each of theories of small and large deformation is applied in solving 3-D thermal-elastic-plastic problem to compare with their efficiency about calculation imes and solution accuracy. When solid elements are used in a bending problem of a plate, phenomenon that the predictive deformation is more than that of actual survey is observed. To prevent this phenomenon, reduced integration method for element is employed instead of full integration that is generally used in 3-D thermal-elastic-plastic analysis.

• Journal of Korean Society on Water Environment
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• v.25 no.6
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• pp.972-978
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• 2009

This study modifies the present total maximum daily load (TMDL) system of Ministry of Environment and applies to the Anyangcheon watershed. Hydrologic Simulation Program-FORTRAN (HSPF) model is used to simulate both runoff and non-point source pollution, simultaneously, instead of QUAL2E. The drought flow (355th daily flow) is proposed for the target water quantity since it is easier to satisfy low flow (275th daily flow) for the target water quality than drought flow. The increase of discharge is more than the increase of pollutant load except for the period under low flow. The measured unit loads for non-point source are used to consider the regional runoff characteristics. The measured water quantity and quality data are used since the ministry of environment supports only water quality. This analysis results show some reasons for the improvement of the present TMDL system of Korea.