• KIISE Transactions on Computing Practices
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• v.22 no.2
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• pp.77-82
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• 2016

This paper presents the development of the Korean medicine symptom diagnosis system. In the Korean medicine symptom diagnosis system, the patient explains their symptoms and an oriental doctor makes a diagnosis based on the symptoms. Natural language processing is required to make a diagnosis automatically through the patients' reports of symptoms. We use morphological analysis to get understandable information from the natural language itself. We developed a diagnosis system that consists of NoSQL document-oriented databases-MongoDB. NoSQL has better performance at unstructured and semi-structured data, rather than using Relational Databases. We collect patient symptom reports in MongoDB to refine difficult medical terminology and provide understandable terminology to patients.

• Korean Journal of Agricultural Science
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• v.45 no.4
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• pp.715-723
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• 2018

The present study was done to investigate the effect of co-culturing muscle satellite cells (MSCs) and intramuscular preadipocytes (IPs) on the differentiation of adipocytes and muscle cells isolated from Korean native cattle. MSCs and IPs were single-cultured in 10% fetal bovine serum/Dulbecco's modified Eagles medium (FBS/DMEM) for 48 h followed by culturing in 5% FBS/DMEM as the growth media. Then, the growth media was replaced by differentiation media composed of 2% FBS/DMEM without any additives for the single- or co-culture of muscle cells and intramuscular adipocytes to induce the differentiation of both cell types. Cell differentiation was measured by morphological investigation and cytosolic enzyme analysis of glycerol-3-phosphate dehydrogenase (GPDH) for the adipocytes and creatine kinase (CK) for the muscle cells. In the morphological test, the presence of muscle cells did not stimulate adipocyte differentiation showing more differentiation of the adipocytes in the single-culture compared to the co-culture condition. However, the differentiation of muscle cells was promoted by adipocytes in the co-culture. The results of the enzymatic analysis were highly associated with the morphological results with a statistically higher GPDH activity (p < 0.05) appearing in the single-culture than in the co-culture, whereas the opposite was true for the CK activity of the muscle cells (p < 0.05). By manipulating in vivo the milieu using a co-culture, we could detect the difference in the rate of cell differentiation and suggest that a co-culture system is a more reliable and precise technique compared to a single-culture. Further studies on various co-culture trials including supplementation of differentiating substances, gene expression analysis, etc. should be done to obtain practical and fundamental data.

• Korean Journal of Plant Taxonomy
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• v.37 no.4
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• pp.477-502
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• 2007

The Aconitum alboviolaceum Kom. complex includes four controversial species described from Korea; A. albouiolaceum, A. pseudolaeue, A. longecassidatum, and A. quelpaertense. The main objective of this study was to determine the taxonomic identities and systematic relationships among the species in the A. albouiolaceum complex based on morphology, numerical analyses and DNA sequence analysis. In the present study, variations in the principal morphological characters and chloroplast DNA noncoding region sequences (psbA-trnH IGS, trnL intron, and trnL-trnF IGS) were examined for 95 individuals from 20 populations. Also, neighbor-joining analysis was adopted to infer their relationships. Morphological variation appeared to be considerably high but not to be related to geographic distribution. These morphological results suggest that reevaluation of key morphological characters are needed for the proper taxonomic treatment of the complex. The length of psbA-trnH IGS region ranged from 241 to 250 bp, that of the trnL intron from 526 to 532 bp, and that of the trnL-trnF IGS region from 466 to 472 by in all taxa. Nine haplotypes were recognized from the analysis. Seven populations shared more than two haplotypes, while other thirteen populations shared only one haplotype. In the phylogenetic analysis, the nine haplotypes formed four groups, separated A. sibiricum, one of the sister groups of the complex. There also was no distinct grouping pattern supporting the species and populations observed. These results suggest that introgression or speciation might have been involved in the formation of taxa of A. alboviolaceum complex.

• Korean Journal of Medicinal Crop Science
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• v.18 no.6
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• pp.379-388
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• 2010

Adenophora racemosa is recently reported as a new Korean endemic plant species. However, the phylogenetic relationship of this genus has been controversial due to the morphological similarity and frequent morphological change of aerial parts. To verify the phylogenetic position of Adenophora racemosa and phylogenetic relationship of genus Adenophora, we analyzed the internal transcribed spacer (ITS) sequence of nuclear ribosomal DNA (nrDNA) and random amplified polymorphic DNA (RAPD) using 21 individual of 6 Adenophora species, A. verticillata, A. divaricata, A. racemosa, A. remotiflora, A. stricata and A. tetraphylla. In comparative analysis of the nrDNA-ITS sequences, we could not found not only any species specific nucleotide sequence but also could not estimated their inter or intra species. In the phylogenic analysis based on the RAPD derived DNA polymorphism, Adenophora species were classified into four groups by clustering analysis of the UPGMA. These results suggest that the DNA fingerprinting based on RAPD is more suitable than nrDNA-ITS sequence for the phylogenetic analysis of Adenophora species.

• Journal of Biosystems Engineering
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• v.38 no.1
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• pp.55-63
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• 2013

Purpose: The present study aimed at image processing methods for automatic cell pattern recognition and morphological analysis for tissue engineering applications. The primary aim was to ascertain the novel algorithm of adaptive brightness correction from microscopic images for use as a potential image analysis. Methods: General microscopic image of cells has a minor problem which the central area is brighter than edge-area because of the light source. This may affect serious problems to threshold process for cell-number counting or cell pattern recognition. In order to compensate the problem, we processed to find the central point of brightness and give less weight-value as the distance to centroid. Results: The results presented that microscopic images through the brightness correction were performed clearer than those without brightness compensation. And the classification of mixed cells was performed as well, which is expected to be completed with pattern recognition later. Beside each detection ratio of hBMSCs and HeLa cells was 95% and 92%, respectively. Conclusions: Using this novel algorithm of adaptive brightness correction could control the easier approach to cell pattern recognition and counting cell numbers.

• Journal of Periodontal and Implant Science
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• v.52 no.6
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• pp.479-495
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• 2022

Purpose: Rodent models have emerged as an alternative to established larger animal models for peri-implantitis research. However, the construct validity of rodent models is controversial due to a lack of consensus regarding their histological, morphological, and biochemical characteristics. This systematic review sought to validate rodent models by characterizing their morphological changes, particularly marginal bone loss (MBL), a hallmark of peri-implantitis. Methods: This review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. A literature search was performed electronically using MEDLINE (PubMed), and Embase, identifying pre-clinical studies reporting MBL after experimental peri-implantitis induction in rodents. Each study's risk of bias was assessed using the Systematic Review Center for Laboratory animal Experimentation (SYRCLE) risk of bias tool. A meta-analysis was performed for the difference in MBL, comparing healthy implants to those with experimental peri-implantitis. Results: Of the 1,014 unique records retrieved, 23 studies that met the eligibility criteria were included. Peri-implantitis was induced using 4 methods: ligatures, lipopolysaccharide, microbial infection, and titanium particles. Studies presented high to unclear risks of bias. During the osseointegration phase, 11.6% and 6.4%-11.3% of implants inserted in mice and rats, respectively, had failed to osseointegrate. Twelve studies were included in the meta-analysis of the linear MBL measured using micro-computed tomography. Following experimental peri-implantitis, the MBL was estimated to be 0.25 mm (95% confidence interval [CI], 0.14-0.36 mm) in mice and 0.26 mm (95% CI, 0.19-0.34 mm) in rats. The resulting peri-implant MBL was circumferential, consisting of supra- and infrabony components. Conclusions: Experimental peri-implantitis in rodent models results in circumferential MBL, with morphology consistent with the clinical presentation of peri-implantitis. While rodent models are promising, there is still a need to further characterize their healing potentials, standardize experiment protocols, and improve the reporting of results and methodology.

• KSTLE International Journal
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• v.2 no.1
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• pp.35-39
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• 2001

In this paper, statistical approach is undertaken to investigate the classification of wear debris which is the key function of objective assessment of wear debris morphology. Wear tests are run to produce various kinds of wear debris. The images of wear debris from wear tests are captured with image acquisition equipment. By thresholding, two-dimensional binary images of wear debris are made and, then, morphological parameters are used to quantify the images of debris. Parametric and nonparametric discriminant method are employed to classify wear debris into predefined wear conditions. It is demonstrated that classification accuracy of parametric and nonparametric discriminant method is similar. The selected use of morphological parameters by stepwise discriminant analysis can generally improve the classification accuracy of parametric and nonparametric discriminant method.

• ALGAE
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• v.20 no.4
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• pp.325-331
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• 2005

Populations of Campylaephora borealis (Nakamura) Seo, Cho et Boo and C. crassa (Okamura) Nakamura show a year-around occurrence of all life-history stages. Such a concurrency of life-history stages produces problems in recognizing species in the field. Here, we invesitgated the morphological variation and life-history stages of both species using a statistical character analysis. Life-history stage was correlated with the seawater temperature in C. borealis, whereas it was dependant on biomass in C. crassa. Thalli had dichotomous branches with adaxial branchlets. The statistics showed that the seasonal change in morphology of C. borealis was significantly different from that of C. crassa in seven qualitative characters and five quantitative characters (p < 0.001), although six quantitative features including tetrasporangial size were similar in both species. The morphological difference between the two species may be due to the annual variation of branchlet number and the variance of branch subangle.

• KSTLE International Journal
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• v.3 no.1
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• pp.54-59
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• 2002

It can be effective on failure diagnosis of oil-lubricated tribological system to analyze operating conditions with morphological characteristics of wear debris in a lubricated machine. And it can be recognized that results are processed threshold images of wear debris. But it is needed to analyse and identify a morphology of wear debris in order to predict and estimate a operating condition of the lubricated machine. If the morphological characteristics of wear debris are identified by the computer image analysis and the neural network, it is possible to recognize the friction condition. In this study, wear debris in the lubricating oil are extracted from membrane filter (0.45 ${\mu}m$) and the quantitative value fur shape parameters of wear debris was calculated through the computer image processing. Four shape parameters were investigated and friction condition was recognized very well by the neural network.

• Tribology and Lubricants
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• v.18 no.2
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• pp.147-152
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• 2002

The morphological analysis of wear particle is a very effective means fur machine condition monitoring and fault diagnosis. In order to describe morphology of various wear particle, the wear test was carried out under friction experimental conditions. And fractal descriptors was applied to boundary and surface of wear particle with image processing. These descriptors to analyze shape and surface of wear particle are shape fractal dimension and surface fractal dimension. The boundary fractal dimension can be derived from the boundary profile and surface fractal dimension can be determined by sum of intensity difference of surface pixel. The morphology of wear particles can be effectively obtained by two fractal parameter.