• 한국토양비료학회지
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• 제49권1호
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• pp.44-52
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• 2016

Plants response to phosphate starvation include the changes of activity of some enzymes, such as phosphatases, fructose-1,6-bisphosphatase, sucrose-phosphate synthase and nitrate reductase. In this study, to determine the effects of phosphate starvation on the change of activities of acid and alkaline phosphatase, fructose-1,6-bisphosphatase, sucrose-phosphate synthase, and nitrate reductase were studied in melon seedlings (Cucumis melo L.). The content of the protein and chlorophyll tended to relatively reduced in melon seedlings subjected to phosphate starvation. Acid phosphatase activity in first and second leaves of melon seedlings was relatively higher than that of third and fourth leaves of seedlings in 14 days after phosphate starvation treatment, respectively. Active native-PAGE band patterns of acid phosphatase in melon leaves showed similar to activities of acid phosphatase, whereas alkaline phosphatase activity was different from the change in the activity of acid phosphatase. Inorganic phosphate content in melon seedlings leaves was constant. The changes of Fructose-1,6-bisphosphatase and sucrose phosphate synthase activities showed similar patterns in melon seedlings leaves, and between these enzymes activities and phosphate nutrition negatively related. Fructose-1,6- bisphosphatase and sucrose phosphate synthase activities showed significant difference in second and fourth leaves, but nitrate reductase showed significant difference in first and second leaves in 14days after phosphate starvation treatment. We concluded that phosphate nutrition could affect the distribution of phosphate, carbon and nitrogen in melon seedlings.

• Journal of Plant Biotechnology
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• 제45권3호
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• pp.217-227
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• 2018

Gummy stem blight (GSB) is one of the most destructive and economically important, soil borne diseases of melon caused by the ascomycete fungus, Didymella bryoniae throughout the world. In Korea, however, no GSB resistant genotype has been reported yet. The study aimed to identify GSB resistant melon germplasm. We screened a total of 60 genotypes including 16 lines and 44 melon cultivars collected from USA and Korea. Among the 16 melon lines, four lines including 'PI482399', 'PI140471', 'PI136170' and 'PI420145', and two Korean cultivars viz. 'Asia Papaya' and 'Supra' showed complete resistance. We were aware that both genotypic and environmental variations could influence the phenotypic screening of resistance and susceptibility. We therefore, further assessed all genotypes using 20 SSR markers. The SSR marker 'CMCT505' linked to Gsb1 in chromosome 1 perfectly grouped resistant and susceptible lines indicating that resistance is probably due to the presence of Gsb1 gene. Cloning and sequencing of resistant and susceptible Gsb1 amplicons showed that there were 32-bp deletions in resistant line and 39-bp deletions in resistant cultivar compared to susceptible one. Thus, the resistant melon lines and cultivars identified in this study could be recommended for the melon breeding program. Furthermore, the SSR marker 'CMCT505' which is tightly linked with Gsb1 could be used for molecular screening of melon germplasm.

• 한국환경농학회지
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• 제39권1호
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• pp.20-25
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• 2020

BACKGROUND: Marine algae is a productive organism that is consumed as a nutritious food. However, large amounts of unused portions of the algae are incinerated as trash or dumped in the sea, causing pollution. Recycling algae is important for saving resources and conserving the environment. In this study, the fucoidan which is a major carbohydrate of marine algae was tested as a source of fertilizer for farming. METHODS AND RESULTS: The growth rate of the melon was examined after treating fucoidan and the melon growth factors, weight and length of stem were measured. To discover the mechanism of melon growth promotion of fucoidan, bacteria that decomposed fucoidan were isolated from soil and abalone. Bacillus wiedmannii and Stenotrophomonas pavanii were isolated from terrestrial soil and Pseudomonas sp. was isolated from abalone. Among these three bacteria, Pseudomonas sp. had the highest and most specific fucoidan-decomposing activity. When Pseudomonas sp. was treated with fucoidan on melon-growing soil, the growth of melon was relatively improved compared to the treatment with fucoidan alone. CONCLUSION: We found that fucoidan, the main carbohydrate of marine algae, promoted melon growth. Fucoidan-decomposing microorganisms were isolated from terrestrial soil and marine organism, and we found that these bacteria stimulated the effect of melon growth promotion of marine algae. This is the first report that confirms the fertilizer effect of marine algae and shows the use of bacteria with marine algae.

• 농촌계획
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• 제27권2호
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• pp.43-48
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• 2021

In this study, an analysis was performed using the growth data and environment data of Korean melon farmers to confirm the influence of environmental factors variables on fruit weight of Korean melon. The analysis results can be summarized as follows. First, it was confirmed that humidity and temperature were recognized as the most important factors among the core factors of korean melon farm production management. Second, The correlation analysis of fruit weight and environmental factors showed a statistically significant soil temperature, internal humidity. Third, The Pooled OLS model estimation results showed that the estimation coefficient for soil temperature is (-), and the estimation coefficient for soil temperature square is (+), indicating that optimal control temperature exists.

• Journal of Biosystems Engineering
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• 제33권4호
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• pp.239-247
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• 2008

Firmness is very important factor to evaluate the freshness and ripeness among the various factors, in concerning about the melon quality. This study was carried out to develop the technique using impact signal to measure the melon firmness nondestructively. Results of analyzing impulse signals of melons having different firmness levels showed that the firmness of melon affected various impulse responses including amplitude, transmitted time, maximum peak frequency, firmness index 1 ($f^2m$), and firmness index 2 ($f^2m^{2/3}$). Impulse signal amplitude was the best indicator to predict the firmness of melon because of a strong corelation ($R^2\;=\;0.9071$). Firmness index 1, firmness index 2, maximum peak frequency, and normalized transmitted time were also possible indicators with acceptable correlation values.

• 대한임상검사과학회지
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• 제51권4호
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• pp.504-513
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• 2019

본 연구의 목적은 Streptozotocin에 의한 당뇨 유발 흰쥐에 대한 여주 분말의 항당뇨병 및 항산화능 효과를 알아보기 위한 것이다. 정상 및 대조군에 AIG-93M식이를 공급하였고, 여주첨가 군에는 1%, 2%, 3%의 여주 분말을 공급하였다. 2주 후 Streptozotocin 투여로 당뇨를 유발하였다. 동물 희생 후 혈당조절과 항산화능을 분석하였다. 여주 첨가군의 혈당 수준은 모두 대조군보다 낮았으며, 2% 여주 첨가군은 대조군보다 유의하게 낮은 결과를 보였다. 2%, 3% 여주 첨가군의 혈청 트리글리세라이드와 고밀도지질단백질-콜레스테롤 수치는 대조군보다 유의하게 낮은 결과가 나왔다. 당뇨 유발 군의 혈청 인슐린은 정상군보다 유의하게 낮았다. 당화혈색소는 2% 및 3% 여주첨가 군이 대조군보다 유의하게 낮은 결과를 보였다. 간 조직에서 항산화 효소의 수치는 2% 여주첨가군이 대조군보다 유의하게 높게 나타났다. 이러한 결과는 당뇨병 예방 및 당뇨병 치료를 위한 여주 분말의 항당뇨병 및 항산화 효과를 나타내는 것으로 보여진다.

• 한국미생물·생명공학회지
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• 제51권4호
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• pp.535-537
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• 2023

We report the draft genome sequence of Bacillus thuringiensis serovar aizawai AS23, an insecticidal strain targeting lepidopteran pests, which was isolated from the rhizosphere of Korean melon (Cucumis melo L.). The genome of strain AS23 comprising 6,846,584 bp with a G + C content of 34.83% was assembled to 11 contigs obtained using hybrid assembly. Additionally, we mined the genome for pesticidal genes, identifying several insecticidal genes, including Cry1Aa3, Cry1Ca9, Cry1Da2, Cry1Ia44, Cry2Ab41, Cry9Ea9, Spp1Aa1, and Vip3Aa86.

• 식물병연구
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• 제16권3호
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• pp.254-258
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• 2010

Melon necrotic spot virus (MNSV)는 국내에서 재배되고 있는 멜론(Cucumis melo)에 심각한 피해를 주고 있다. 2009년 전남 나주 멜론재배단지에서 괴저반점을 나타내는 멜론 잎에서 MNSV를 분리 동정하였다. 지표식물 반응, immuno captured(IC) RT-PCR 및 염기서열 상동성 비교 등 몇몇 분석으로 이 분리주를 MNSV-Me로 명명하였다. 멜론 시판 35품종에 대한 MNSV-Me의 저항성 선발을 위하여 유묘의 자엽에 인위적 즙액 접종 방법을 이용하였다. 접종된 모든 품종의 자엽에서는 괴저반점을 형성하였다. 이들 중 25품종은 상엽으로 괴저 증상의 이행 또는 일부 개체에서는 접종 약 40일 후 줄기에 3 cm 이상의 괴저를 나타내는 감수성 반응을 나타냈다. 5품종은 상엽으로 잘 이행이 되지 않지만 줄기에 3 cm 미만의 괴저증상을 형성하는 중간 저항성을 발현하였다. 접종한 상엽으로는 바이러스 증상이 발현되지 않고, 단지 줄기에 희미한 괴저가 형성되는 얼스타이탄, 얼스럭서리, 베타리치 하계, 베타리치 및 원더플하계1호가 저항성을 나타냈다.

• Journal of Plant Biotechnology
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• 제46권2호
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• pp.71-78
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• 2019

The objective of this study was to use 'Danta PR', NGS (Next Generation Sequencing) technology for genome resequencing to develop polymorphic makers between Chinese oriental melon, 'Hyangseo 1' and Korean oriental melon. From the resequencing data that covered about 81 times of the genome size, 104,357 of SSR motifs and Indel, and 1,092,436 of SNPs were identified. 299 SSR and 307 Indel markers were chosen to cover each chromosome with 25 markers. These markers were subsequently used to identify genotypes of 'Danta PR' BC1 (F1 x 'Danta PR') population and a genetic linkage map was constructed. SSR, Indel, and SNPs identified in this study would be useful as a breeding tool to develop new oriental melon varieties.

• 한국환경농학회지
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• 제28권3호
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• pp.281-288
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• 2009

Azoxystrobin과 kresoxim-methyl의 참외 중 반감기와 잔류양상을 조사하였다. Azoxystrobin의 참외 중 0일차 잔류량은 기준량 및 배량 처리구에서 각각 0.09 및 0.14 mg/kg으로 나타났으며 농약의 반감기 소실곡선식은 y=0.0766e$^{-0.138x}$ ($r^2$=0.9424) 및 y=0.1143e$^{-0.0890x}$ ($r^2$=0.9310) 이었으며 생물학적 반감기는 각각 5.0일 및 7.8일 이었다. Kresoxim-methyl의 참외 중 0일차 잔류량은 기준량 및 배량 처리구에서 각각 0.10 및 0.23 mg/kg으로 나타났으며 농약의 소실곡선식은 y=0.0896e$^{-0.1672x}$ ($r^2$=0.9428) 및 y=0.1504e$^{-0.1446x}$ ($r^2$=0.9040) 이었고 생물학적인 반감기는 각각 4.1일 및 4.8일 이었다. 재배기간 중 참외의 무게증가에 농약희석효과를 배제한 절대잔류농도는 약제 살포 후 14일 경과시 azoxystrobin은 기준량 및 배량에서 0.01 및 0.05 mg/kg으로서 각각 83.6 및 67%의 농약이 분해되었다. Kresoxim-methyl은 각각 0.01 및 0.03 mg/kg 으로서 86.2 및 87.8% 정도의 농약 분해율을 보였다.