• The Korean Journal of Physiology and Pharmacology
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• 제19권4호
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• pp.319-325
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• 2015

Among solute carrier proteins, the organic anion transporters (OATs) play an important role for the elimination or reabsorption of endogenous and exogenous negatively charged anionic compounds. Among OATs, SLC22A9 (hOAT7) transports estrone sulfate with high affinity. The net decrease of estrogen, especially in post-menopausal women induces rapid bone loss. The present study was performed to search the SNP within exon regions of SLC22A9 in Korean females with osteoporosis. Fifty healthy controls and 50 osteoporosis patients were screened for the genetic polymorphism in the coding region of SLC22A9 using GC-clamped PCR and denaturing gradient gel electrophoresis (DGGE). Six SNPs were found on the SLC22A9 gene from Korean women with/without osteoporosis. The SNPs were located as follows: two SNPs in the osteoporosis group (A645G and T1277C), three SNPs in the control group (G1449T, C1467T and C1487T) and one SNP in both the osteoporosis and control groups (G767A). The G767A, T1277C and C1487T SNPs result in an amino acid substitution, from synonymous vs nonsynonymous substitution arginine to glutamine (R256Q), phenylalanine to serine (F426S) and proline to leucine (P496L), respectively. The Km values and Vmax of the wild type, R256Q, P496L and F426S were 8.84, 8.87, 9.83 and $12.74{\mu}M$, and 1.97, 1.96, 2.06 and 1.55 pmol/oocyte/h, respectively. The present study demonstrates that the SLC22A9 variant F426S is causing inter-individual variation that is leading to the differences in transport of the steroid sulfate conjugate (estrone sulfate) and, therefore this could be used as a marker for certain disease including osteoporosis.

• Korean Journal of Acupuncture
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• 제40권3호
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• pp.128-133
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• 2023

Objectives : There are many variations in the ST36 acupoint location. The purpose of this article is to suggest a method of locating the ST36 acupoint. Methods : Based on the available research and the neuroanatomical characteristics of the underlying acupoint, we summarized the proper procedure for finding the ST36 acupoint. Results : ST36 is 3 B-cun inferior to ST35 and is vertically situated on the line that connects ST35 and ST41. The ST36 acupoint corresponds to the deep peroneal nerve, which is situated in the tibialis anterior muscle's back. The neurovascular bundles that are located on the interosseous membrane between the interosseous crests of the tibia and fibula include the deep peroneal nerve, anterior tibial artery, and anterior tibial vein. According to both classical and modern literature, this acupoint can be found horizontally between the two muscles, tibialis anterior and extensor digitorum longus. Conclusions : Based on a review of the literature and neuroanatomical features, we suggest that ST36 can be positioned horizontally between tibialis anterior and extensor digitorum longus. Additional imaging studies and clinical proof are required to determine ST36 acupoint.

• 한방재활의학과학회지
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• 제33권4호
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• pp.243-249
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• 2023

해면상 혈관종(cavernous hemangioma)은 인체 내 다양한 장기나 기관에서 발생할 수 있는 혈관기형으로, 순수 경막외 해면상 혈관종(pure epidural cavernous hemangioma)은 해면상 혈관종의 드문 경우에 속한다. 해면상 혈관종이 원인이 되어 발생한 증상에 한의학 치료를 적용한 연구가 드물게 보고되었으나, 순수 해면상 혈관종의 출혈로 인한 증상에 대한 것은 없었기에 관련 임상증례를 보고하고자 한다. 척추의 순수 해면상 혈관종으로 진단받은 51세 남성이 2022년도에 해면상 혈관종 출혈로 인해 발생한 보행장애에 대해 치료를 받기 위해 한방병원을 방문하였다. 척추의 순수해면상 혈관종 출혈로 인해 발생한 보행장애 환자는 침 치료, 전기 침 치료, 온침 치료, 한약, 추나, 뜸, 부항 치료를 시행 받았다. 평가지표는 25 foot walk test (25FW), timed up and go test (TUG), comfortable gait speed tests (CGS), 요통과 하지 통증에 대한 numeral rating scale (NRS), Berg balance score (BBS), Tinneti-score, manual muscle test (MMT), EuroQol-five dimension (EQ-5D), EQ-5D visual analog scale (EQ-5D VAS)이었으며, 25FW, TUG, CGS, NRS, BBS, EQ-5D (VAS)에서는 치료 후 호전을 보인 반면, Tinetti Score, MMT, EQ-5D의 지표는 치료 후에도 동일하게 유지되었다. 한의학은 척추 해면상 혈관종의 출혈로 인해 발생한 보행장애나 하지 강직감에 대한 치료의 한 방안으로 고려될 수 있다.

• 대한한방부인과학회지
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• 제31권1호
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• pp.147-154
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• 2018

Objectives: This study aims to examine the improvement against facial wrinkles after needle-embedding therapy. Methods: The study participants were 10 women treated with needle-embedding therapy in the Korean Medical Clinic from September, 2011 to August, 2014. The subjects were treated every ten days for twenty days, totally three times, and the result of treatment was evaluated five times, such as pre-treatment, after each treatments, one month and two months later of the last treatment with ARAMO-SG. Results: 1. Depth and range of facial wrinkles, facial skin pore test, and sensitivity test were visibly improved after needle-embedding therapy and follow-up compared pre-treatment (p<0.05). 2. The significant improvements in facial skin texture test was not observed and sustained until the follow-up measurement at 2 months. 3. Any adverse reaction related to needle-embedding therapy did not happen. Conclusions: This study suggests that needle-embedding therapy can improve facial wrinkles. The positive results of this study support the requirement for additional research investigating the efficacy of needle-embedding therapy in women.

• 한방재활의학과학회지
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• 제33권4호
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• pp.15-29
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• 2023

Objectives The purpose of this study is to systematically review the effects of Neuromuscular Electrical Stimulation treatment on muscle wasting in cancer patients. Methods Randomized controlled trials (RCTs) were searched from seven online databases (PubMed, Cochrane Library, EMBASE, China National Knowledge Infrastructure, Oriental Medicine Advanced Searching Integrated System, Korean studies Information Service System, Research Information Sharing Service. The selected RCTs were evaluated for methodological quality through the Cochrane RoB. Results A total of 126 articles were identified, and 4 randomized controlled trials were selected for systematic review. In one study, it was found that there were statistically significant improvements in the Health-Related Quality of Life (FACTH&N total score, p<0.001). Additionally, significant effects were observed in measurements that represented the size of the quadriceps muscle (cross-sectional area of Vastus lateralis and Rectus femoris, p=0.004), maximum muscle strength, the twitch response of resistance muscles, and voluntary activation (p<0.001). However, no significant differences were observed between the intervention and control groups in terms of quadriceps muscle strength in two other studies (p>0.05). Lastly, while one study showed no significant differences in muscle fiber characteristics between the two groups, it did report significant improvements in measurements related to mitochondria within muscle tissue and muscle strength in the intervention group (p<0.05). Conclusions Neuromuscular electrical stimulation can be a method used to improve muscle strength in muscle wasting of cancer patients, but it is difficult to see its effects as significant compared to other treatments.

• Journal of Preventive Medicine and Public Health
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• 제5권1호
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• pp.105-110
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• 1972

On 6 healthy adult males aged 20-30 years, oxygen pulse was observed during carrying sand bags weighing 10, 20 and 30 kg on a level treadmill running with speeds of 3, 4 and 5 km/hr. The results obtained were as follows. 1. Oxygen pulse during load carrying on a level treadmill was proportionately increased with an increase of work load, average oxygen uptake per minute. Regression equation of oxygen pulse (y: $m\iota$/beat) on the average oxygen uptake (x:$\iota$/min) required for each grade of work was expressed as y=3.34x+5.99, $s_{y.x}=0.858$). 2. Oxygen pulse reached the highest value 2-5 minutes after the start of load carrying, and thereafter it decreased gradually to some extend as the load carrying was continued. Rate of decrease in the oxygen pulse was greater in lighter works. 3. In lighter works requiring less than $2.0\iota/min$ of oxygen uptake, no difference in oxygen pulse could be seen between methods of carrying even when the same amount of load was carried. In heavier works requiring more than $2.0\iota/min$ of oxygen uptake, oxygen pulse was larger in case of carrying a load by embrace, in both hands and on head than on back or on shoulder.

• Journal of Preventive Medicine and Public Health
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• 제17권1호
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• pp.281-287
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• 1984

In search for a method of evaluating the cardiopulmonary function. 74 male and 33 female volunteers ages $18{\sim}25$ were subjected to this study The subjects walked on a treadmill at speed of 2,4,6 and 8km/hr with 0,5,10,15,20 and 25% grade of inclination, respectively, for a measurement of heart rate and oxygen-pulse. Heart rate was measured every 5 seconds at resting state and during walking by telemetric method using Heart Checker 108 System (Senoh Co., Japan). Oxygen concentration was measured by Douglas bag method collecting expired air for 5 minutes at rest, and for 2 minutes at the end of each walking exercise. Oxygen concentration in an expired air was analyzed with Orzat gas analyser and expressed in terms of STPD. Oxygen-pulse was defined as an amount of oxygen consumed at every heart at a cellular level. The followings were the results obtained from this study. 1. Mean values of oxygen-pulse at resting state was $3.1{\pm}0.11ml/beat$ in male and $2.5{\pm}0.87ml/beat$ in female, respectively. 2. Mean values of oxygen-pulse during treadmill walking were increased in proportion with the load of exercise, namely, the speed and grade of inclination, from minimum of 7.1ml/beat upto maximum of 18.2ml/beat in male and from minimum of 4.2ml/beat upto maximum of 12,7ml/beat in female. 3. Both linear and logarithmic regressional relationships between oxygen-pulse and speed of walking and grade of inclination were observed in both sexes. Predicted values of oxygen-pulse by logarithmic regressional formula on speed and on grade of inclination were better coincided with the measured values than those predicted by the linear regressional formula.

• 한국인터넷방송통신학회논문지
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• 제20권5호
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• pp.9-13
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• 2020

사상의학은 개인별 맞춤의학에 최적화된 한국 고유의 전통의학으로 유효한 임상 효능에도 불구하고 체질진단의 부정확도는 한계로 지적받아 왔다. 체질 진단의 정확도를 개선하기 위해 정량 데이터에 기반한 체질 분석 알고리즘이 개발되었으며, 본 연구에서는 개발된 알고리즘을 적용한 체질 분석 체험 시스템을 개발하고 반복성을 평가하였다. 체질분석 체험 시스템은 정면과 측면 안면 영상, 음성, 설문 정보를 수집하고 통합 체질 확률 값을 계산하여 체험자의 체질을 분석한다. 개발한 시스템의 체질 확률 값에 대한 반복도 평가를 위해 3명을 대상으로 각 5회 반복 측정하였으며, 변동계수가 4.778%로 나와 충분한 반복도를 보이는 것을 확인하였다. 개발한 시스템은 사상의학의 인식 제고를 위한 홍보에 기여할 수 있을 것이다.

• Journal of Oral Medicine and Pain
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• 제45권4호
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• pp.97-109
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• 2020

Purpose: Cervicogenic headache (CGH) is pain referred to the head/ face from the structures in vicinity of upper cervical spinal nerves via trigeminocervical pathway. Ponticulus Posticus (PP) and Elongated Styloid Process (ESP) are anatomical structures that cause compression of vasculature present around upper cervical nerve plexus. Recently, computational fluid dynamics (CFD) has shown to play an essential role in identification of these high-pressure zones in the brain. The aim of this research is to study the association of ESP and PP in patients with CGH and to develop a hypothesis by CFD to analyse vertebrobasilar insufficiency as a contributing factor in occurrence of CGH. Methods: Retrospective analysis of 4500 full skull CBCT scans was done for the presence of partial or complete PP and length of Styloid Process (SP). Research was divided into two phases; In first Preliminary Phase, 150 scans that showed the presence of PP and ESP were analysed, and only 134 patients gave consent to fill the questionnaire containing 96 question items pertaining to symptoms associated with CGH. In the second phase, simulation of Vertebral and Carotid Artery was done using Fluent 14.5 Software and by CFD, pressure distribution on arteries was obtained that helped to identify high pressure regions. Results: Both PP and ESP showed a statistically significant association with CGH (p<0.001). By CFD analysis, both steady and transient phases of simulation showed drop in pressure due to constriction of internal carotid and vertebral artery by ESP and PP respectively and were found to decrease the volume of blood reaching the brain, 0.12 /0.13 mL and 0.06 mL respectively. Conclusions: Our analysis proves ESP and PP as contributing factors towards CGH. Hence for proper diagnosis and management of headache disorders, clinicians should have adequate knowledge about these anatomical structures and their resulting clinical symptoms.

• 대한한의학원전학회지
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• 제36권2호
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• pp.77-96
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• 2023

Objectives : The purpose and application method of the five kinds of Gagamjeong-gisan in the Onbyeongjobyeon were analyzed and their significance was examined. Methods : First, contents within medical texts on the Gwakhyangjeong-gisan, the original formula for the Gagamjeong-gisan were analyzed. Next, application, symptoms and pathogenic mechanism, treatment principle, composition of the Gagamjeong-gisan as written in the Onbyeongjobyeon were analyzed. Characteristics of each formula as deduced from this process were compared and examined. Results : The Gwakhyangjeong-gisan covers both external contraction and internal damage, applied regardless of external or internal symptoms. It restores the Yang qi of the Spleen and Stomach, balancing the Zheng qi to respond to the external qi. The Gagamjeong-gisan takes after the Gwakhyangjeong-gisan, reflecting such properties. However, the latter differs in that it excludes ingredients unfit for treating dampness-warmth, and uses different application methods according to the level of discharge of dampness pathogen. Conclusions : The Gagamjeong-gisan is modified from the Gwakhyangjeong-gisan, which is applied to cold damage and internal damage to better treat warm disease. It can be said to have acted as a bridge for warm disease scholars in composing formulas that deal with dampness-heat disease.