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Profiling of flavonoid glycosides in fruits and leaves of jujube (Zizyphus jujuba var. inermis (Bunge) Rehder) using UPLC-DAD-QTOF/MS (UPLC-DAD-QTOF/MS를 이용한 대추나무(Zizyphus jujuba var. inermis (Bunge) Rehder) 잎과 열매의 플라보노이드 배당체 분석)

  • Lee, Min-Ki;Kim, Heon-Woong;Kim, Young Jin;Lee, Seon-Hye;Jang, Hwan-Hee;Jung, Hyun-Ah;Kim, Sook-Bae;Lee, Sung-Hyen;Choe, Jeong-Sook;Kim, Jung-Bong
    • Food Science and Preservation
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    • v.23 no.7
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    • pp.1004-1011
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    • 2016
  • Flavonoids, non-nutrient secondary metabolites of plants, are widely distributed in commonly consumed agro-food resources. Flavonoids include aglycones, and their glycosides are reported to have potential health-promoting compounds. The aim of this study was to investigate flavonoid glycosides in the fruit and leaves of Zizyphus jujuba var. inermis (Bunge) Rehder (jujube). A total of six flavonoids (five flavonols and one chalcone) were identified in jujube fruit and leaves by using ultra-performance liquid chromatography-diode array detector-quadrupole time of flight mass spectrometry along with chemical library and an internal standard. In positive ion mode, six flavonoids were linked to the C- and O-glycosides which were conjugated with sugar moieties based on kaempferol, quercetin, and phloretin aglycones. Total flavonoid contents of leaves (8,356.5 mg/100 g dry weight (DW)) was approximately 900-fold higher than that of fruit (fresh fruit, 13.6 mg/100 g dry DW; sun-dried fruits, 9.2 mg/100 g dry DW). Quercetin 3-O-rutinoside (rutin) and quercetin 3-O-robinobioside were the predominant flavonols in fruit and leaves of jujube. In particular, rutin had the highest content (6,735.2 mg/100 g DW) in leaves, and rutin is a widely reported bioactive compound. Phloretin 3',5'-di-C-glucoside (chalcone type) was detected only in leaves. The leaves of jujube contain a high content of flavonoids and the results of this study indicate that jujube leaves may be a source of bioactive flavonoids.

Cloning of Cytochrome P450 Gene involved in the Pathway of Capsidiol Biosynthesis in Red Pepper Cells (고추세포에서 Capsidiol 생합성을 유도하는 Cytochrome P450 유전자의 탐색)

  • Kwon, Soon-Tae;Kim, Jae-Sung;Jung, Do-Cheul;Jeong, Jeong-Hag;Hwang, Jae-Moon;Oh, Sei-Myoung
    • Journal of Life Science
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    • v.13 no.6
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    • pp.879-888
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    • 2003
  • In order to measure the enzyme activity of 5-epi-aristolochene hydroxylase, one of cytochrome P450 (P450) enzymes in eicitor-treated pepper cell, we used in vivo assay method and demonstrated a dramatic suppression of the activity by P450-inhibitors, ancymidol and ketocornazole. Using RT-PCR method with degenerate primer of the well conserved domains found within most P450-enzymes, and using cDNA library screening method, one distinct cDNA, being designated P450Hy01, was successfully isolated from elicitor-treated pepper cells. P450Hy01 mRNA was all induced in elicitor-treated cells whereas never induced in control cells. Moreover, levels of P450Hy01 expression were highly correlated with the levels of extracellular capsidiol production by different elicitors in cell cultures. P450Hy01 transcript was also induced by several other elicitors such as, cellulase, arachidonic acid, jasmonic acid, yeast extract as well as UV stress. P450Hy01 sequence contained high probability amino acid matches to known Plant P450 genes and ORF with a conserved FxxGxRxCxG heme-binding domain. P450Hy01 cDNA showed 98% of homology in sequence of nucleotide as well as amino acid to 5-epi-aristolochene-1, 3-hydroxylase (5EAl, 3H) which has been isolated in tobacco cells, suggesting that P450Hy01 is prominent candidate gene for P450-enzyme encoding 5EAl, 3H in pepper cell.

Characteristics of Scientific Method for the 8th Grade Students‘ Inquiry Reports (8학년 학생들의 탐구 보고서에 나타난 과학방법의 특징)

  • Shin, Mi-Young;Choe, Seung-Urn
    • Journal of the Korean earth science society
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    • v.29 no.4
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    • pp.341-351
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    • 2008
  • The purpose of this study was to investigate eighth graders' scientific method of inquiry used in their reports. We developed a framework, 'Analysis of Scientific Methods and Information Sources', with a perspective of the Nature of Science to analyze students' planning method, data analysis, and information sources. We then compared results with levels of questions to find out whether they affected students' 'Scientific Method'. In addition, we analyzed students' responses of the survey questionnaire, e.g.. how they liked Scientific Method. Results are as follows: First, 'planning method' consisted of 'consultant' and 'activities'. The 'activities' were 'experiment', 'correlational study', and 'observation' Students planned by utilizing 'consultant' more than the other. In case of planning 'activities'. most of them were 'experiment' Second, 'data analysis' consisted of 'summary', 'table', 'chart', 'graph' and so on. Students analyzed their data by using 'summary' frequently. The types of 'summary' were divided into 'simple summary' and 'relational statement' Third, 'information sources' consisted of 'computer', 'library'. and 'professional consultant' Most of the students gathered information from 'computer' Fourth, the types of 'planning method' and 'summary' were affected by the levels of questions. Fifth, some of the students reported their difficulty in 'planning method' because the collected information was less reliable, lacking, and having difficult technical terms.

A Study on Automatic Generation of Interface Circuits Based on FSM between Standard Buses and Ips (FSM을 이용한 표준화된 버스와 IP간의 인터페이스 회로 자동생성에 관한 연구)

  • Lee, Ser-Hoon;Moon, Jong-Uk;Hwang, Sun-Young
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.30 no.2A
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    • pp.137-146
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    • 2005
  • IP-based design methodology has been popularly employed for SoC design to reduce design complexity and to cope with time-to-market pressure. Interface modules for communication between system buses and IPs are required, since many IPs employ different protocols. Automatic generation of these interface modules would enhance designer's productivity and IP's reusability. This paper proposes an automatic interface generation system based on FSM generated from the protocol description of IPs. The proposed system provides the library modules for the standard buses to reduce the burdens of describing the protocols for data transfer from/to standard buses. Experimental results show that the area of the interface circuits generated by the proposed system had been increased slightly by 4.5% on the average when compared to manual designs. In the experiment, where bus clock is 100 Mhz and slave module clock is 34 Mhz, the latency of the interface had been increased by 7.1% in burst mode to transfer 16 data words. However, occupation of system bus can be reduce by 64.9%. A chip designer can generate an interface that improves the efficiency of system bus, by using this system.

Design of an Improved Anti-Collision Unit for an RFID Reader System Based on Gen2 (Gen2 리더 시스템의 개선된 충돌방지 유닛 설계)

  • Sim, Jae-Hee;Lee, Yong-Joo;Lee, Yong-Surk
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.2A
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    • pp.177-183
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    • 2009
  • In this paper, we propose an improved anti-collision algorithm. We have designed an anti-collision unit using this algorithm for the 18000-6 Type C Class 1 Generation 2 standard (Gen2). The Gen2 standard uses a Q-algorithm for incremental method on the Dynamic Slot-Aloha algorithm. It has basically enhanced performance over the Slot-Aloha algorithm. Unfortunately, there are several non-clarified parts: initial $Q_{fp}$ value, weighted C, and the ending point of the algorithm. If an incorrect value is selected, it causes degradation in performance. Thus we propose an improved anti-collision algorithm by clearly defining the vague parts of the existing algorithm. Simulation results showed an improved performance of up to 34.8% using an optimized value of C and the initial $Q_{fp}$ value. With the ending condition, performance is 34.7%. The anti-collision unit is designed using the Verilog HDL. The module was synthesized using Synopsys' Design Compiler and the TSMC $0.2{\mu}m$ standard cell library. The synthesized result yielded 3,847 gates, and was guaranteed under the proposed working frequency of 19.2MHz.

A Research on the Use of Information Resources of the Humanities Scholars in Korea (인문학자의 정보자원 이용에 관한 연구)

  • Yoon, Cheong-Ok
    • Journal of the Korean Society for information Management
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    • v.26 no.1
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    • pp.7-34
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    • 2009
  • The purpose of this study is to investigate the use of information resources of the humanities scholars in Korea and suggest the strategic planning of academic library and information services to serve their needs. To collect data, a mail survey was conducted during the period of November 2007 through January 2008. Out of 799 humanities scholars sampled from 25 universities, 132 responded with the completion rate of 16%. Major findings in this study are as follows: First, the majority of the humanities scholars distribute their time equally to research and education, and conduct independent research. Second, they still largely depend on print resources, including scholarly journals, books, and manuscripts for getting information, while using some online information resource mostly in text such as electronic documents and journals. They expect that the frequency of using such print resources would not decline much, but the use of online resources could definitely increase in the next five years. Some variances in the behavior were observed among the groups of scholars according to their research experiences. It is suggested that academic libraries (1) conduct more active outreach, (2) prioritize between traditional and electronic resources, (3) plan information literacy education for traditional resources.

Development of a Gridded Simulation Support System for Rice Growth Based on the ORYZA2000 Model (ORYZA2000 모델에 기반한 격자형 벼 생육 모의 지원 시스템 개발)

  • Hyun, Shinwoo;Yoo, Byoung Hyun;Park, Jinyu;Kim, Kwang Soo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.19 no.4
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    • pp.270-279
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    • 2017
  • Regional assessment of crop productivity using a gridded simulation approach could aid policy making and crop management. Still, little effort has been made to develop the systems that allows gridded simulations of crop growth using ORYZA 2000 model, which has been used for predicting rice yield in Korea. The objectives of this study were to develop a series of data processing modules for creating input data files, running the crop model, and aggregating output files in a region of interest using gridded data files. These modules were implemented using C++ and R to make the best use of the features provided by these programming languages. In a case study, 13000 input files in a plain text format were prepared using daily gridded weather data that had spatial resolution of 1km and 12.5 km for the period of 2001-2010. Using the text files as inputs to ORYZA2000 model, crop yield simulations were performed for each grid cell using a scenario of crop management practices. After output files were created for grid cells that represent a paddy rice field in South Korea, each output file was aggregated into an output file in the netCDF format. It was found that the spatial pattern of crop yield was relatively similar to actual distribution of yields in Korea, although there were biases of crop yield depending on regions. It seemed that those differences resulted from uncertainties incurred in input data, e.g., transplanting date, cultivar in an area, as well as weather data. Our results indicated that a set of tools developed in this study would be useful for gridded simulation of different crop models. In the further study, it would be worthwhile to take into account compatibility to a modeling interface library for integrated simulation of an agricultural ecosystem.

Bacillus subtilis를 이용한 대두 발효식품의 혈전용해능

  • Jeong, Yeong-Gi
    • Proceedings of the Korean Society of Life Science Conference
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    • 2001.06a
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    • pp.67-86
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    • 2001
  • A strain producing strongly fibrinolytic enzyme was isolated from soil and was identified to be Bacillus subtilis by biochemical and physiological characterization. The optimal culture conditions for the production of fibrinolytic enzyme was determined to be 1.0% tryptone, 1.5% soluble starch, 0.5% Peptone, 0.5% NaCl, $(NH_{4})_{3}PO_4.3H_{2}O, and MgSO_{4}.7H_{2}O.$ Initial pH and temperature were pH 8.0 and $30^{\circ}C$ , respectively, The highest enzyme production was observed at 30 hours of cultivation at $30^{\circ}C$ The fibrinolytic enzyme was purified to homogeneity by DEAE Sephadex A-50 ion exchange column chromatography, 70% ammonium sulfate precipitation, Sephadex G-200 and G-75 gel filtration column chromatography. The molecular weight of the purified enzyme was 28,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A gene encoding the fibrinolytic enzyme was cloned into a plasmid vector pBluescript, transforming E.coli XL-1 Blue. The clone was able to degrade fibrin, This indicated that the gene could encode a fibrinolytic enzyme. The nucleotide sequence of the 2.7 kb insert was determined in both direction. One open reading frame composed of 1023 nucleotides was found to be a potential protein coding region. There was the putative Shine-Dalgano sequence and TATA box upstream of the open reading frame. The homology search data in the genome database showed that both the 2.7 kb insert and 1 kb open reading frame carried no significance in the nucleotide sequence of known fibrinolytic enzyme from Bacillus serovars. The recombinant cell harboring the novel gene involved in fibrinolysis was subjected to protein purification. The molecular mass of the purified fibrinolytic enzyme was determined to be 31864 Dalton, which was highly in accordance with the molecular mass(33 kDa) of the fibrinolytic gene deduced from the insert. The fibrinolytic enzyme was Purified 50.5 folds to homogeneity in overall yield of 10.7% by DEAE Sephadex A-50 ion exchange, 85% ammonium sulfate precipitation, Sephadex G-50, Superdex 75 HR FPLC gel filtration. In conclusion, a novel fibrinolytic gene from Bacillus subtilis was identified and characterized by cloning a genomic library of Bacillus subtilis into pBleuscript. For the soybean fermented by this strain, it is found that there increased assistant protein about 20% compared to the soybean not fermented and increased about 30% according to amino acid analysis and, in particular, essential amino acid increased about 40%. When keeping this fermented soybean powder at room temperature for about 70days, it showed very high stability maintaining almost perfect activity and, therefore, it gave us great suggestion its possibility of development as a new functional food.

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New Paradigm in exhibition organization at the National Museum of Contemporary Art ('연구 업무 전담제'를 통해 살펴보는 국립현대미술관 전시 기획의 새로운 패러다임)

  • Choi, Eun-Ju
    • The Journal of Art Theory & Practice
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    • no.3
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    • pp.67-84
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    • 2005
  • Since the evaluation of its intellectual activities and abilities is done by curator's capabilities, planning exhibition is very important as the final result achieved by their own knowledge, information, and research. ARPA(Advanced Research Project on Arts) is suggested as the system which enables curators responding simultaneously to the society in the times, based on its special characteristics. If this system settles well, which means that the curators at NMCA(National Museum of Contemporary Art, Korea) play their roles as the professionals in each of their fields, the goal of consolidating the status of museum as the representative national museum, and building up competent department of curators, will be achieved at the same time. To clarify above, the curators set up the various assignments of research about the types of arts such as painting, Korean painting, sculpture, installation, new-media, design, craft, photogarphy, architecture, etc. And they establish the art objects classified by the regions, such as the Northern American, Southern American, European, Asian, and other Third World countries. They elaborate art objects more on the history, the work, the artist, and the issue of contemporary art. Furthermore, when the curators devote deeper study to those research subjects, they can have the opportunities to design an exhibition upon the research. Today, the museum of art is 'The Place for Communication and Encounter', it is regarded important to share the aesthetical, creative values with current artists, and to understand mutually with the spectators. It is needed to improve the curator's work, in order to meet the demands of the times and even to advance. Because the form of 'exhibition' is the tool that reveals the identity NMCA aiming at, the motivation, the development, and the realization should be leaded by the curators, who are the mainstream of the museum. ARPA is a system for identifying the exhibition like mentioned above. The main purpose of this system is to produce synergy effect, having the researching, collecting work in liaison with planning exhibition. ARPA will be able to improve the quality of exhibition through the way of developing the exhibition, passing through the stable process in the long run. So far, I have referred to a new paradigm of the exhibition design at NMCA via ARPA. Yet, there still remain missions in reality, such as analyzing the previous exhibition and reshuffling personnel and system, which should be done. When these matters settled, these plans would be suggested practically. At this point, it is the most significant that NMCA is attempting to let others aware of the importance of exhibition planning based on research. when the ARPA and exhibition planning is conjoined together successfully, the competent exhibition will be achieved, which can offer a meaningful exhibition to the art world, strengthen infra structure thru exchanging with public museum in the region, and eventually, establish a network with museum in foreign countries.

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Isolation and Characterization of Helicobacter pylori Urease Inhibitor from Rubus coreanus Miquel (복분자(Rubus coreanus Miquel)로부터 Helicobacter pylori Urease Inhibitor의 분리 및 특성)

  • 양성우;호진녕;이유현;신동훈;홍범식;조홍연
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.5
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    • pp.769-777
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    • 2004
  • A Helicobacter pylori urease inhibitor from Rubus coreanus Miquel has been isolated and partially characterized for aiming to Prevent H. pylori growth and decrease harmful accumulation of ammonia in human gastric mucosa. We screened urease inhibitory activities in 519 extracts library prepared by solvent extraction from 173 kinds of edible plants, medicinal herbs, herbs and seaweeds using a colorimetric urease assay system. As results of primary and secondary screening, 70% acetone extract of Rubus coreanus Miquel was selected as potent candidate, showing about 24% inhibitory activity. The acetone extract was sequentially partitioned into RCE/RCWI and RCB/RCW2 layers with ethyl acetate and butanol. The major active component in RCW2, water layer from butanol fractionation was revealed to be peptidic or proteinous substance by inhibitory activity determination after pronase digestion and periodate oxidation. RCW2-IIIc a was isolated by sequential column chromatography on DEAE-Toyopearl 650C, Butrl-Toyopearl 650M and Sephadex LH-20. The isolated urease inhibitor RCW2-IIIc $\alpha$, was highly pure proteinous substance with molecular weight of 13kDa by high-performance gel permeation liquid chromatography. RCW2-IIIc$\alpha$ has about 5 times higher inhibitory activity than 70% acetone extract, showing high stability against heat treatment and peptic digestion.