• Biomolecules & Therapeutics
• /
• 제26권3호
• /
• pp.313-321
• /
• 2018

Anti-cancer drug resistance is a major problem in colorectal cancer (CRC) research. Although several studies have revealed the mechanism of cancer drug resistance, molecular targets for chemotherapeutic combinations remain elusive. To address this issue, we focused on the expression of cellular prion protein ($PrP^C$) in 5-FU-resistant CRC cells. In 5-FU-resistant CRC cells, $PrP^C$ expression is significantly increased, compared with that in normal CRC cells. In the presence of 5-FU, $PrP^C$ increased CRC cell survival and proliferation by maintaining the activation of the PI3K-Akt signaling pathway and the expression of cell cycle-associated proteins, including cyclin E, CDK2, cyclin D1, and CDK4. In addition, $PrP^C$ inhibited the activation of the stress-associated proteins p38, JNK, and p53. Moreover, after treatment of 5-FU-resistant CRC cells with 5-FU, silencing of $PrP^C$ triggered apoptosis via the activation of caspase-3. These results indicate that $PrP^C$ plays a key role in CRC drug resistance. The novel strategy of combining chemotherapy with $PrP^C$ targeting may yield efficacious treatments of colorectal cancer.

• 대한한의학회지
• /
• 제30권2호
• /
• pp.88-103
• /
• 2009

Objectives: There is currently increased interest in the identification of natural antioxidant compounds derived from various plants. Peony Root (PR) is used worldwide for the treatment of many types of cardiovascular disease including atherosclerosis and hypertension. It has been used in Korean traditional medicine for the treatment of glycosuria, hypertension and cancer. However, to date, no studies concerning the antioxidant properties of PR have been conducted. Therefore, this study was conducted to evaluate the in vitro scavenging activity, inhibitory effect of LDL oxidation of pro-oxidant reactive species and anti-thrombosis effect in response to treatment with PR using various screening methods including biological and non-biological oxidants. Methods: In this study, the antioxidant activity of extract from PR was studied with in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$-induced human LDL oxidation and the inhibitory effect on collagen-induced platelet aggregation. Results: The PR extracts were found to have a potent scavenging activity of oxidative stress [DPPH, superoxide anion, hydroxyl radical, nitric oxide and peroxynitrite, etc.] as well as an inhibitory effect on LDL oxidation and on platelet aggregation. Conclusions: The PR extracts have anti-oxidative and anti-atherosclerotic effects in vitro system, which can be used for developing pharmaceutical drugs against oxidative stress and atherosclerosis.

• Journal of Applied Biological Chemistry
• /
• 제63권4호
• /
• pp.393-399
• /
• 2020

방풍(Saposhnikoviae Radix, SR) 과 식방풍(Peucedani Japonici Radix, PR)은 각각 다른 기원식물을 가지며 속과 종이 다르지만 한국, 중국, 및 일본에서 대표적인 전통 한약재로 오랫동안 혼용되어 사용되었다. 본 연구에서는 방풍과 식방풍의 판별 마커를 확인하기 위하여 UPLC-QTOF/MS를 이용한 대사체 프로파일링 및 다변량 통계분석을 진행하였다. 그 결과, 5-O-methylvisammioside와 peucedanol을 각각 방풍과 식방풍의 지표성분으로 선정하였으며, UPLC를 이용하여 분석법을 검증하였다. 제안된 방풍 및 식방풍의 지표성분에 대한 분석법 검증은 방풍과 식방풍의 분류와 품질 평가 및 성분 검증을 위한 효과적인 방법이 될 것으로 기대된다.

• 한국정밀공학회지
• /
• 제26권6호
• /
• pp.36-40
• /
• 2009

Development of maskless lithography techniques can provide a potential solution for the photomask cost issue. Furthermore, it could open a market for small scale manufacturing applications. Since femtosecond lasers have been found suitable for processing of a wide range of materials with sub-micrometer resolution, it is attractive to use this technique for maskless lithography. As a femtosecond laser has recently been developed, both of high power and high photon density are easily obtained. The high photon density results in photopolymerization of photoresist whose absorption spectrum is shorter than that of the femtosecond laser. The maskless lithography using the two-photon absorption (TPA) makes micro structures. In this paper, we present a femtosecond laser direct write lithography for submicron PR patterning, which show great potential for future application.

• 한방안이비인후피부과학회지
• /
• 제35권4호
• /
• pp.106-122
• /
• 2022

Objectives : In this study, we aimed to analyze the latest knowledge of pityriasis rosea(PR) through oriental-western medical review on PR. Methods : We searched Pubmed, CNKI, and OASIS to select papers related to the cause, mechanism, diagnosis, classification, treatment, and herbal treatment of PR. Results : The results are as follows. 1. Although the exact cause of PR is not known, it has recently been found that reactivation of latent human herpesvirus-6 and human herpesvirus-7 infection is a possible etiology. Most patients require emollients, antihistamines, topical steroids, and macrolides. Acyclovir, and narrow-band UVB therapy are also used. 2. The cause and mechanism of PR in Chinese and Korean medicine can be mainly summarized as wind-heat, blood heat, and blood deficiency and wind-dryness. Most of the herbal medicine used have the effects of clearing heat, cooling the blood, detoxifying, dispelling wind, relieving itching, nourishing blood, and moistening dryness. Conclusions : In Chinese and Korean medicine, it is necessary to organize the representative prescriptions according to pattern identification, and it is also necessary to study the atypical types of PR and its treatment methods. It is thought that research on oriental and western combination therapy should be continuously conducted, and efforts to develop Korean medicine formulations are needed to revitalize clinical research in Korea.

• Journal of Ginseng Research
• /
• 제39권4호
• /
• pp.406-413
• /
• 2015

Background: Pathogenesis-related 10 (PR-10) proteins are small, cytosolic proteins with a similar three-dimensional structure. Crystal structures for several PR-10 homologs have similar overall folding patterns, with an unusually large internal cavity that is a binding site for biologically important molecules. Although structural information on PR-10 proteins is substantial, understanding of their biological function remains limited. Here, we showed that one of the PgPR-10 homologs, PgPR-10.3, shares binding properties with flavonoids, kinetin, emodin, deoxycholic acid, and ginsenoside Re (1 of the steroid glycosides). Methods: Gene expression patterns of PgPR-10.3 were analyzed by quantitative real-time PCR. The three-dimensional structure of PgPR-10 proteins was visualized by homology modeling, and docking to retrieve biologically active molecules was performed using AutoDock4 program. Results: Transcript levels of PgPR-10.3 expressed in leaves, stems, and roots of 3-wk-old ginseng plantlets were on average 86-fold lower than those of PgPR-10.2. In mature 2-yr-old ginseng plants, the mRNA of PgPR-10.3 is restricted to leaves. Ginsenoside Re production is especially prominent in leaves of Panax ginseng Meyer, and the binding property of PgPR-10.3 with ginsenoside Re suggests that this protein has an important role in the control of secondary metabolism. Conclusion: Although ginseng PR-10.3 gene is expressed in all organs of 3-wk-old plantlets, its expression is restricted to leaves in mature 2-yr-old ginseng plants. The putative binding property of PgPR-10.3 with Re is intriguing. Further verification of binding affinity with other biologically important molecules in the large hydrophobic cavity of PgPR-10.3 may provide an insight into the biological features of PR-10 proteins.

• 한국조경학회지
• /
• 제33권5호통권112호
• /
• pp.83-93
• /
• 2005

Research was initiated to investigate turf performance under USGA soil system. USGA system 45 centimeters deep was built with rootzone layer, intermediate layer, and drainage layer. Six turfgrass entries were comprised of 3 blends and 3 mixtures from cool-season grasses (CSG). Turfgrass color and quality ratings were best in spring and fall, especially early May to early July and late August to early November. Kentucky bluegrass (KB) consistently produced the greatest performance, while perennial ryegrass (PR) the poorest. Intermediate turf performance between KB and PR was observed with tall fescue (TF). Among CSG mixtures it increased with KB but decreased with PR. There were considerable variations in summer turf Performance. No summer drought injury was found in KB and TF. However, PR showed poor performance through summer as compared with others. Among mixtures, it decreased with PR. It was suggested that PR mix in less than $20\%$ in the mixtures to have an acceptable quality in summer. Cultural intensity also affected it. With lowering mowing height, KB of rhizomatous-type in growth habit kept good quality, while PR and TF with bunch-type in growth habit poor quality. Mowing quality was greatly different among CSG. KB produced clean-cut surface, but PR unclean one. If had an intermediate mowing quality between KB and PR. A great difference in green color retention was observed among CSG. The longest CSG was PR that kept green for 339 days, while the shortest one TF for 267 days. KB continued to keep green for 290 days. The mixtures kept green in color for 292 to 315 days, depending on turfgrass mixing intensity. The greater the PR in content, the longer the green color duration. These results demonstrate that KB was the best and PR the worst among CSG grown in USGA system under a domestic climate, in regards of turf quality, color, mowing quality, summer turf performance and green color duration. KB and TF are most adequate for high-maintenance and low-maintenance area, respectively. In case of mixtures for high-quality turf, it was desirable to use KB-based mixture with PR of below $20\%$ in seeding rate.

• Journal of Plant Biotechnology
• /
• 제42권1호
• /
• pp.25-33
• /
• 2015

배 검은별무늬병 고도저항성 '93-3-98'과 고도감수성 '스위트스킨'간의 suppression subtractive hybridization 분석을 통해 '93-3-98'에서 특이적으로 발현되는 pathogenesis-related 10 (PR-10) 유전자를 분리하여 PyrcpPR-10으로 명명하고 기관 및 품종별 발현양상을 분석하였다. 단편염기서열의 rapid amplification of cDNA ends PCR을 통해 PyrcpPR-10 유전자는 전체길이가 743bp이고, 480bp의 ORF와 159개의 아미노산을 가지는 것으로 확인되었다. PyrcpPR-10 유전자의 염기서열은 '황실리'(저항성), '감천배'(중도저항성), '원황'(중도감수성), '신고', '스위트스킨'(고도감수성)은 동일하였으나 'Bartlett'(고도저항성)은 일부 염기서열의 차이를 보였다. BLAST X를 통한 다른 식물 종의 PR-10 아미노산과 비교에서 64 ~ 98%의 상동성을 보였고 공통적으로 GXGGXG motif를 가지고 있었다. 기관 및 조직별 PyrcpPR-10 유전자의 발현량은 꽃잎이 가장 높았으며 다음으로 잎, 꽃대, 눈, 수피 순이었다. 저항성과 감수성 품종에 따른 PyrcpPR-10 유전자의 발현양상은 모든 품종에서 접종 24시간 후 급격히 증가였으며, 특히 'Bartlett', '93-3-98', '황실리'에서 높게 발현되었고 '감천배', '원황'의 경우 저항성 품종에 비해 상대적으로 낮았으며, 고도 감수성 '신고', '스위트스킨'은 발현이 가장 낮았다. 배에서 분리한 PyrcpPR-10 유전자는 검은별무늬병 저항성에 직접 연관되는 것으로 추정된다.

• 대한원격탐사학회지
• /
• 제16권4호
• /
• pp.293-303
• /
• 2000

The typhoon Jelawat, which was formed over the tropical Pacific ocean on August 1, 2000 and made a landfall over China on August 10, 2000, was observed by Korea Multi-purpose Satellite (KOMPSAT-1) Ocean Scanning Multispectral Imager (OSMI), Tropical Rainfall Measuring Mission (TRMM)/Precipitation Radar(PR) and Quick Scatterometer (QuikSCAT). In spite of discontinuous observation, important mesoscale features of typhoon depending on life cycle were detected prominently. It is possible to distinguish on the OSMI photograph between the eye-wall convection and the stratiform and other convective clouds near the center of typhoon Jelawat. The TRMM/PR observations show quite clearly the eye-wall convection, stratiform regions, and convective bands. Vertical cross section of rainfall in the genesis stage of typhoon Jelawat exhibits circular ring of intense convection surrounding the eye. The mature stage of typhoon Jelawat consists of a strong rotational circulation with clouds which are well organized about a center of low pressure. The OSMI, TRMM/PR and QuikSCAT measurements presented here agree qualitatively with each other and provide a wealth of information on the structure of typhoon Jelawat.

• 한국콘텐츠학회논문지
• /
• 제17권9호
• /
• pp.107-119
• /
• 2017

최근 광고, PR 분야는 새로운 도전에 직면하고 있는데 새로운 디지털 콘텐츠 시장 성장과 패러다임의 변화가 일어나고 있기 때문이다. ICT(정보통신 : Infomation and Communication Technology) 및 디지털 콘텐츠 시장에서도 가장 빠르게 성장하고 있는 분야 중 하나가 VR(Virtual Reality) 등의 기술과 관련된 실감형 분야라고 할 수 있다. 하지만 급변하는 VR 콘텐츠 시장에서 어떠한 광고, PR 전략으로 참여해야 하는지에 대한 명확한 해답을 찾기 힘들다. 그러므로 본 연구에서는 국내 VR 콘텐츠 시장을 이끌고 있는 VR 전문가 11명과의 심층면접을 통해 VR 광고, PR 시장에 대한 P(정치)E(경제)S(사회)T(기술)분석을 하여 VR 산업의 거시적인 면을 분석하였다. 또한 VR 시장의 S(강점)W(약점)O(기회)T(위협) 분석을 통해 대내외적으로 광고, PR 분야의 VR 콘텐츠 활용가능성을 탐색해 보았다. 연구결과 광고, PR 분야에서 VR을 활성화하기 위해서는 먼저 킬러 콘텐츠를 육성하고, 이용자의 니즈에 맞춰 불편한 점을 개선해야 하며, 소비자 및 업계의 인식을 개선하여야 하는 것으로 나타났다. 또한 지속적인 광고, PR 관련 VR 활성화 정책이 마련되어야 하며, 마지막으로 중장기 관점의 VR 로드맵을 구축해야 하는 것으로 나타났다. 따라서 본 연구는 이제 막 태동하는 VR 콘텐츠 시장에서 광고, PR 분야의 VR 콘텐츠 전략 방향을 제공하고, 향후 VR 활성화를 위한 연구에 기초를 제공하고자 한다.