• 제목/요약/키워드: Korean Goat

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PCR 기법을 이용한 한국재래산양 β-casein 유전자의 특성 (Characteristics of β-casein Gene using the PCR Technique in Korean Native Goat)

  • 김지애;류승희;유성란;이준헌;서길웅;김선균;상병찬
    • 농업과학연구
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    • 제29권2호
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    • pp.43-52
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    • 2002
  • 본 연구는 한국 재래 산양 112두와 유산양인 Saanen종 7두의 혈액으로부터 genomic DNA를 추출하고, PCR-RFLP 방법에 의해 $\beta$-casein 유전자의 특성을 분석하여 한국재래산양의 효율적인 유전자원의 보존 및 개량을 위한 기초 자료로 제공하고자 실시하였다. 한국재래산양의 genomic DNA로부터 PCR기법을 이용하여 $\beta$-casein의 유전자좌를 증폭한 결과 각각 481bp 크기의 단편이 양호하게 증폭되었음을 확인하였다. $\beta$-casein 유전자 좌의 증폭산물에 대한 Bal I의 제한효소를 처리한 결과, $\beta$-casein AB형은 481bp, 284bp 및 197bp의 단편을, 그리고 BB형은 284bp와 197bp의 단편을 한국재래산양과 유산양인 Saanen 종에서 확인 할 수 있었다. 유전자형 빈도에 있어서는 한국재래산양에서 $\beta$-casein AB 및 BB의 빈도는 각각 6.25 및 93.75%이었고, 유산양인 Saanen 종은 각각 57.14 및 42.86%이었다. 유전자빈도에 있어서는 한국재래산양의 $\beta$-casein A 및 B의 빈도가 각각 0.031 및 0.969이었고, Saanen 종에서는 각각 0.286 및 0.714의 빈도를 보였다. 한국재래산양의 $\beta$-casein 유전자의 염기서열과 이미 보고되어 있는 goat의 염기서열(GeneBank accession Number M90556)간에는 총 11개의 염기서열에 차이를 나타내어 97.71%의 상동성을 보였다. 따라서 한국재래산양의 $\beta$-casein 유전자의 다형성과 염기서열 분석에 의한 분자유전학적 특성의 규명은 한국재래산양의 유전자원의 보존 및 개량을 위한 기초 및 응용 자료로 이용될 수 있을 것으로 생각된다.

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진구수침에크스, 지실수침(枳實水浸)에크스, 및 진구성분(??成分)Lycaconitine의 산양(山羊)진드기 구제효력(驅除效力)에 관한 야외실험(野外實驗) (Field Tests on Repellent Effects against Korean Goat Ticks of Aqueous Extracts of Jingyu and Fructus Ponciri, and also of Lycaconitine, an Alkalold of Radix Jingyu)

  • 이장락
    • 대한수의학회지
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    • 제2권2호
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    • pp.25-27
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    • 1962
  • It was previously reported that aqueous extracts of Radix Jingyu and Fructus ponciri, and also Lycaconitine, an alkaloid of Radix Jingyu, exhibit excellent effects as cattle tick repellent. The repost deals with the field teats carried out with these three drugs for their repellent effects against Korean goat ticks, nearly 99 per cent of which are composed of Haemaphysalis bispinosum and the rest of Boophilus microplus. The study revealed that Korean goat ticks are as susceptible to all three drugs tested as korean cattle ticks.

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한국재래산양의 눈신경 가지에 관한 해부학적 연구 (Anatomical study on the branches of nervus ophthalmicus of the Korean native goat)

  • 이인세;이흥식;이성준
    • 대한수의학회지
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    • 제32권4호
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    • pp.457-462
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    • 1992
  • This study was carried out to investigate the branches and distribution of Nervus ophthalmicus of the Korean native goat. The observation was made by dissection of embalmed cadavers of ten Korean native goats. The results were as follows; 1. N. ophthalmicus arised from the trigeminal nerve in a common trunk with the maxillary nerve. 2. At the exit of the foramen orbitorotundum the N. ophthalmicus gave off Ramus zygomaticotemporalis, N. frontalis, Ramus sinuum frontalium and N. nasociliaris. 3. Ramus zygomaticotemporalis, after giving off N. lacrimalis, left the orbital cavity and gave off Rami cornuales which were distributed the caudolateral part of the base of the horn. 4. After giving off N. ciliaris longus and Ramus communicans cum ganglio ciliari, N. nasociliaris divided into N. ethmoidalis and N. infratrochlearis which gave off Rami cornuales.

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한국재래산양 뇌두개의 분리골에 대한 해부학적 연구 (Anatomical study on the individual bones of the cranium of Korean native goat)

  • 이성준;이흥식
    • 대한수의학회지
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    • 제34권3호
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    • pp.421-433
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    • 1994
  • This study was carried out to identify anatomical characteristics of the individual bones of the cranium of the Korean native goat. The pars lateralis and pans basilaris of the os occipitale were fused each other, whereas the squama occipitalis articulated with the pars lateralis, forming the sutrue. The sinus sphenoidale was formed in the corpus ossis presphenoidalis. The os prietale was observed as a single bone. In the os frontale, an fossa innominatum was found ventrolateral to the root of processus cornualis, and the orificium orbitale canalis supraorbitalis was also formed on the pars orbitalis. The meatus temporalis was formed in the medial side of the pars squama of the os temporalis. The lamina cribrosa of the os ethmoidale was membrane-like structure with many foramina. The labyrinth ethmoidalis was composed of four endoturbinalia and ten to twelve ectoturbinalia.

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경북지역 재래산양의 세균성, 바이러스성 설사병 병원체 검출률 조사 (Detection ratio of bacterial and viral pathogens of diarrhea from Korean indigenous goat feces in Gyeongbuk province)

  • 손준형;도재철;조길재
    • 한국동물위생학회지
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    • 제39권1호
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    • pp.35-39
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    • 2016
  • The purpose of this study was to survey on infection status of pathogens of diarrhea from Korean indigenous goat. A total of 800 fecal samples was collected from 50 farms from January to October 2015 and was tested by automatic biochemical machine and polymerase chain reaction (PCR). The overall detection ratio of bacterial pathogens was 22.4% and viral pathogens was 16.3%, respectively. The detection ratio of Escherichia coli (E. coli), Salmonella spp., bovine viral diarrhea virus (BVDV), rotavirus and coronavirus were 21.5%, 0.9%, 7.6%, 5.6% and 3.0%, respectively. In the rates of mixed detection, single was 78.2%, double 8.4%, triple 11.6% and quadruple 1.8% in each sample and 38%, 12%, 16%, 20% in each farm, respectively.

재래산양의 설사병 병원체 감염률 조사 (Status of diarrhea pathogens from Korean indigenous goat feces)

  • 손준형;이재봉;황유선;김상윤;김석환
    • 한국동물위생학회지
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    • 제40권1호
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    • pp.21-25
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    • 2017
  • The purpose of this study was to survey on infection status of pathogens of diarrhea from Korean indigenous goat. A total of 800 fecal samples was collected from 50 farms from January to November 2016 and was tested by automatic biochemical machine and polymerase chain reaction (PCR). The overall infection rates of parasitic, bacterial and viral pathogens was 13.0%, 23.0%, 11.3% and the rates of coccidia, Escherichia coli (E. coli), bovine viral diarrhea virus (BVDV), rotavirus and coronavirus were 13.0%, 23.0%, 5.3%, 8.8% and 2.6%, respectively. In the rates of mixed detection, single was 29.8%, double 5.1%, triple 2.8%, quadruple 1.1% in each sample, respectively.

산양의 Liver cirrhosis 발생보고 (Liver Cirrhosis of Korean Native Goat in Korea : A Case Report)

  • 민병만;박경애;김환균;조용성;김성열;구찬희;정운익;김홍집
    • 한국동물위생학회지
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    • 제16권1호
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    • pp.57-64
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    • 1993
  • This survey was performed to report rare outbreak of liver cirrhosis in Korean native goat (KNG) which was died of Yangpyeong's goat farm on Feb. 1992. The examination for the KNG was carried out by clinical signs, necropsy and various lab-oratory test including parasitic, bacterial and histological test. The KNG looked jaundice, ascite, hemorrhage of lumen, abomasum and intestine, and brownish smooth cirrhotic liver at necropsy. Histological examination for liver revealed considerable proliferation of connective tissue and piecemeal necrosis which was caused by chronic active inflammation in interlobules and intralobules. There were atrophic micro and macro nodules which were sur-rounded by connective tissue. The lobular structure lack almost all central vein. The portal areas appearred proliferation of bile ducts, blood vessels and connective tissues. These connective tissue infiltrated heavily with plasma cells, Iymphocytes and histocytes. Histological examination for brain proved to be hepatic encephalopathy by virture of congestion and edema in cerebral medullary. From these results were demonstrated miked nodular, active, postnecrotic liver cirrhosis.

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친환경 흑염소 사양을 위한 최적 조사료 초종 및 TMR 혼합비 비율: In vitro 반추위 발효 연구 (Optimum Forage Sources and Its Ratio in TMR for Environmently-friendly Goat Feeding: In vitro Rumen Fermentation Study)

  • 류채화;이진욱;김관우;이성수;박혜련;전은정;박명선;최낙진
    • 한국유기농업학회지
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    • 제28권4호
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    • pp.605-614
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    • 2020
  • This study was conducted to investigate the effects of TMR on in vitro rumen fermentation and methane production of goat with different forage sources. The experiment was arranged 4×2 factorial design. The different forage sources were rice straw (RS), Italian rye grass (IR), timothy (TI) and alfalfa (AL), respectively. There were two different forage : concentrate ratios such as 20:80 (20) and 50:50 (50), respectively. Therefore, totally 8 treatments were used: 1) RS20, 2) RS50, 3) IR20, 4) IR50, 5) TI20, 6) TI50, 7) AL20, and 8) AL50, respectively. The rumen fluid of goat was collected from the slaughterhouse. For fermentation parameters, ruminal pH, total gas, methane, hydrogen, ammonia nitrogen, and volatile fatty acid were determined. The pH values were within an optimal range across all treatments. Total gas productions at TI20 and AL50 were significantly greater than others (p<0.05). Methane production was significantly lower in TI and AL compared with other treatments (p<0.05). The relatively high dietary NDF content in treatments showed significantly lower methane production (p<0.05). Significant alterations treatments were detected at ammonia nitrogen concentration according to the ratio of forage : concentrate (p<0.05). AL treatment showed greater total volatile fatty acid production compared with other treatments (p<0.05). Therefore, the present study suggests that both Timothy and Alfalfa could be recommendable forage sources for goat based on results with volatile fatty acid as an energy source and methane as an index for energy loss and environmental issues. Also, the 50:50 (forage : concentrate) ratio would prefer to 20:80.

병풀분말을 첨가한 염소고기 떡갈비의 품질특성 (Quality Characteristics of Goat Meat Tteokgalbi Prepared with the Addition of Centella asiatica Powder)

  • 이정아;조수현;김윤석;김현욱;배인선
    • 한국식생활문화학회지
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    • 제38권5호
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    • pp.336-344
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    • 2023
  • This study aimed to investigate the effects of Centella asiatica powder on the quality characteristics and storage stability of goat meat Tteokgalbi. The goat meat Tteokgalbi samples were prepared with four different quantities (0, 0.5, 1, and 1.5%) of Centella asiatica powder. The moisture content of the samples containing Centella asiatica powder was significantly lower than that of the control (p<0.05). The protein content of the samples containing Centella asiatica powder was significantly higher than that of the control (p<0.05). As the proportion of Centella asiatica powder increased, the pH, L*, and a* values of the uncooked and cooked samples decreased (p<0.05). The water holding capacity and the cooking yield of the uncooked and cooked samples were higher in the samples containing Centella asiatica powder than in the control (p<0.05). Also, with the increasing amounts of the Centella asiatica powder, the thiobarbituric acid reactive substance (TBARS) levels decreased. The 1-1.5% Centella asiatica powder group showed lower levels of volatile basic nitrogen (VBN) than the other groups as per the assay (p<0.05). The results indicate that Centella asiatica powder could enhance the quality characteristics and storage stability of goat meat Tteokgabi.

Offsprings Produced by Transcervically Inseminating Frozen-thawed Semen into Uterus of a Estrus-induced Saanen Goat during Non-breeding Season

  • Yong, Hwan-Yul;Kim, Min-Ah;Bae, Bok-Soo;Kim, Seung-Dong;Jo, Shin-Il;Lim, Yang-Mook;Yoo, Mi-Hyun;Ha, Yong-Hee;Oh, Chang-Shik;Kim, Doo-Hee
    • 한국수정란이식학회지
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    • 제25권2호
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    • pp.89-92
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    • 2010
  • We report herein the successful results of estrus induction, sperm cryopreservation and kids born by transcervical insemination of frozen-thawed semen in a Saanen goat. Flugestone acetate (FGA: 60 mg) was inserted into vagina for 15 days. The goat was intramuscularly injected with 400 IU PMSG and 200 IU hCG ($PG600^{(R)}$: Intervet, Korea) a day before withdrawal of the FGA sponge. Follicles and corpora lutea were identified on both ovaries by laparoscopy. Artificial insemination was performed 46 hours after removal of FGA sponge. The concentration of frozen-thawed semen was $3.975{\times}10^8/ml$ and 0.5 ml of frozen-thawed semen was transcervically inseminated into uterine body under anesthesia. Three kids, all females, were born 144 days after artificial insemination. This is the first report producing kids by transcervical insemination of frozen-thawed semen in a Saanen goat of which the estrus was induced by FGA sponges, PMSG and hCG during non-breeding season in Korea.