• Journal of Oral Medicine and Pain
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• v.34 no.3
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• pp.237-245
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• 2009

The purpose of this study was to investigate the clinical efficacy of latex cover developed for dental handpiece on contamination of microorganisms during dental treatment and to determine whether it can be an alternative to conventional sterilization such as autoclaving. E. fecaelis was used as a experimental microorganism instead of oral flora. Experimental bowl with 2 cm of rectangular cavity was fabricated for handpiece operating instead of oral cavity. Latex covers ($Orokeeper^{(R)}$, Orobiotech Co., Korea) and several handpieces were used after sterilization by autoclave. Four experiments were performed to evaluate bacterial contamination related with (1) various parts of dental handpiece, (2) swabbing time with alcohol sponge, (3) postoperative air-water spraying time and (4) consecutive use of latex covers without autoclaving. The results show that face of handpiece uncovered with latex cover was severely contaminated than the covered area and that most bacteria were removed by swabbing face and head area of dental hand-piece and by air-water spraying more than 15 seconds nearly up to the level of sterilization. Conclusively it can be suggested that use of latex cover for handpiece during dental procedure, swabbing with alcohol sponge is air-water spraying for more than 15 seconds after use of dental handpiece should be very useful and practical for prevention of cross infection and should be an alternative method for the sterilization of dental handpiece under some difficult situations not being able to sterilize a handpiece with autoclave.

• Mycobiology
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• v.45 no.3
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• pp.160-171
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• 2017

Larvae of Bradysia agrestis, an insect vector that transports plant pathogens, were sampled from geographically isolated regions in Korea to identify their cutaneous fungal and bacterial flora. Sampled areas were chosen within the distribution range of B. agrestis; each site was more than 91 km apart to ensure geographical segregation. We isolated 76 microbial (fungi and bacteria) strains (site 1, 29; site 2, 29; site 3, 18 strains) that were identified on the basis of morphological differences. Species identification was molecularly confirmed by determination of universal fungal internal transcribed spacer and bacterial 16S rRNA gene sequences in comparison to sequences in the EzTaxon database and the NCBI GenBank database, and their phylogenetic relationships were determined. The fungal isolates belonged to 2 phyla, 5 classes, and 7 genera; bacterial species belonged to 23 genera and 32 species. Microbial diversity differed significantly among the geographical groups with respect to Margalef's richness (3.9, 3.6, and 4.5), Menhinick's index (2.65, 2.46, and 3.30), Simpson's index (0.06, 0.12, and 0.01), and Shannon's index (2.50, 2.17, and 2.58). Although the microbial genera distribution or diversity values clearly varied among geographical groups, common genera were identified in all groups, including the fungal genus Cladosporium, and the bacterial genera Bacillus and Rhodococcus. According to classic principles of co-evolutionary relationship, these genera might have a closer association with their host insect vector B. agrestis than other genera identified. Some cutaneous bacterial genera (e.g., Pseudomonas) displaying weak interdependency with insect vectors may be hazardous to agricultural environments via mechanical transmission via B. agrestis. This study provides comprehensive information regarding the cutaneous microflora of B. agrestis, which can help in the control of such pests for crop management.

• Korean Journal of Plant Resources
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• v.27 no.4
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• pp.293-308
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• 2014

This study was carried out to investigate the vascular plants in the construction site of the National DMZ Native Botanic Garden. The period of survey was from May 2012 to November 2013. Vascular plants based on voucher specimen were summarized as 313 taxa including 79 families, 211 genera, 272 species, 4 subspecies, 32 varieties, 4 forms and 1 hybrids. The rare plant species designated by Korea Forest Service were 8 taxa including Galium boreale L., Eleutherococcus senticosus (Rupr. & Maxim.) Maxim., Eranthis stellata Maxim. and Lloydia triflora (Ledeb.) Baker, etc. Endemic plant species were 4 taxa including Salix koriyanagi Kimura, Clematis trichotoma Nakai, Philadelphus schrenkii Rupr. and Cirsium setidens (Dunn) Nakai. Furthermore, 51 taxa were listed as specific plant species based on phytogeographical in the investigated area. The naturalized plants were recorded as 11 taxa, and their Naturalization Ratio and Urbanization Index were recorded as 3.51%, and 3.43%, respectively.

• Korean Journal of Plant Resources
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• v.26 no.4
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• pp.463-490
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• 2013

This study was carried out to investigate the vascular plants of Namhan River. The period of survey was from March to November on 2012. Vascular plants based on voucher specimen were summarized as 430 taxa including 86 families, 250 genera, 383 species, 2 subspecies, 39 varieties and 6 forms. The rare plant species designated by Korea Forest Service were 13 taxa including Penthorum chinense Pursh, Polygonatum stenophyllum Maxim, and Sparganium erectum L., etc. And the endemic plant species were 8 taxa including Salix koriyanagi Kimura, Clematis trichotoma Nakai, and Carex gifuensis Franch., etc. Furthermore, 56 taxa were listed as specific plant species based on phytogeographical and based on the list of plants which have been approved for overseas delivery, 27 taxa were recorded in the investigated area. The naturalized plants were recorded as 58 taxa, and their Naturalization Ratio and Urbanization Index were recorded as 13.5%, and 18.1%, respectively. 430 taxa listed consists of 174 taxa(40.5%) of pasturing plant, 141 taxa(32.8%) of edible plants, 112 taxa(26.0%) of medicinal plants, 48 taxa(11.2%) of ornamental plants, 11 taxa(2.6%) of stain plants, 7 taxa(1.6%) of fiber plants, 4 taxa(0.9%) of timber plants, 2 taxa(0.5%) of industrial plants and 109 taxa(25.4%) of unknown plants.

• Korean Journal of Plant Resources
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• v.19 no.5
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• pp.592-597
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• 2006

To understand the status of ferns' distribution of Gwangneung forest, flora survey was done during the period of April 2003 to November 2005. The results obtained from this study were as follows: Ferns identified and surveyed were 46 taxa including 9 families 17 genera 43 species 3 varieties were recorded in Gwangneung Forest. Hemicryrtophyte (H) was the most common dormancy from recorded in surveyed area. Epiphyte (E) and Hydatophyte (HH) were calculated as 4.3%, 2.2%, respectively and they were the lower value compared to those of other dormancy form. Consequently, $H-R_{3}-D_{1}-t$ was recorded as the most common life-form of ferns in Gwangneung Forest. Pteridophyta-Quotient (Ptph.-Q.) was calculated as 1.28 and it was the higher value compared to those of other neighboring mountains.

• Journal of Veterinary Clinics
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• v.25 no.5
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• pp.340-345
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• 2008

Malassezia pachydermatis (M. pachydermatis) is a component of the normal cutaneous flora of the dog and atopic dermatitis (AD) is one of the most common diseases associated with Malassezia overgrowth in dogs. The purpose of this study was to investigate the humoral response (IgG) to extracts of M. pachydermatis of in a dog with AD. We used Western immunoblotting to identify allergens of M. pachydermatis. Gel electrophoresis of extracts proteins and immunoblotting of sera samples in both an atopic dog and a non-atopic dog were compared. Proteins of 18, 21, 26, 32, 34, 38, 40, 42, 46, 58, 64, 75, 85, and 120 kDa were observed in a serum of atopic dog. However, when serum of a non-atopic dog was used, protein bands were not identified except binding in 50 kDa protein. The results of this study indicate that atopic dogs with M. pachydermatis dermatitis may induce IgG response and also suggest that humoral response to M. pachydermatis could be important in pathogenesis of AD in dogs. However, further studies are required to identify roles of humoral response to M. pachydermatis in canine AD.

• Weed & Turfgrass Science
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• v.4 no.4
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• pp.279-287
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• 2015

The present research was carried out in order to investigate the occurrence of problematic weed species in Cnidium officinale Makino. Field in South Korea. Total 53 sites of the 3 different regions in S. Korea were investigated from May to October, 2014. In Cnidium officinale fields, the identified weeds were distributed in 35 families and 99 species. Total 5 communities that consist of Commelina communis, Eleocharis kuroguwai, Persicaria vulgaris, Chenopodium album-Acalypha australis, and Galinsoga ciliata dominated the appearance. The weeds occurred in Cnidium officinale fields were divided into three groups in principal component plot analysis (PCA). It was observed that in control weeds plots; 20 plants of Cnidium officinale fresh weight is 739.9 g while the uncontrolled plots have no Cnidium officinale plants. The current investigation could be useful for estimation of future weeds occurrence, weed flora dynamics and establishment of weed control methods in Cnidium officinale fields in Korea.

• Journal of Life Science
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• v.25 no.2
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• pp.189-196
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• 2015

Bacterial diversity was studied in the rhizosphere of Suaeda japonica Makino, which is native to Suncheon Bay in South Korea. Soil samples from several sites were diluted serially, and pure isolation was performed by subculture using marine agar and tryptic soy agar media. Genomic DNA was extracted from 29 pure, isolated bacterial strains, after which their 16S rDNA sequences were amplified and analyzed. Phylogenetic analysis was performed to confirm their genetic relationship. The 29 bacterial strains were classified into five groups: phylum Firmicutes (44.8%), Gamma proteobacteria group (27.6%), Alpha proteobacteria group (10.3%), phylum Bacteriodetes (10.3%), and phylum Actinobacteria (6.8%). The most widely distributed genera were Bacillus (phylum Firmicutes), and Marinobacterium, Halomonas, and Vibrio (Gamma proteobacteria group). To confirm the bacterial diversity in rhizospheres of S. japonica, the diversity index was used at the genus level. The results show that bacterial diversity differed at each of the sampling sites. These 29 bacterial strains are thought to play a major role in material cycling at Suncheon Bay, in overcoming the sea/mud flat-specific environmental stress. Furthermore, some strains are assumed to be involved in a positive interaction with the halophyte S. japonica, as rhizospheric flora, with induction of growth promotion and plant defense mechanism.

• Journal of Life Science
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• v.23 no.3
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• pp.341-346
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• 2013

The disappearance of seaweed flora in some rocky areas, which is known as algal whitening, barren ground, coralline flats, or deforested areas, is associated with some species of coralline algae. To determine the biological characteristics of a representative species of crustose coralline alga, the 18S rDNA gene was sequenced to identify the genus Lithophyllum. According to its morphological and distributional characteristics, it was deduced to be L. yessoense. Viability was measured using triphenyl tetrazolium chloride and showed high viability from December to February. Culture conditions of $16^{\circ}C$, a 16 hr light, 8 hr dark cycle, and 30 ${\mu}E/m^2/s$ light intensity were optimal for maintaining the viability of the alga for up to five days. Included in the fatty acids was 9.7% ${\omega}$-3 eicosapentaenoic acid. An electron microscopy scan of the surface structure revealed round craters about 3.6 ${\mu}m$ in diameter, which were covered with rough, irregular, and angular polygon-shaped structures about 1.0 to 3.7 ${\mu}m$ in size. Based on the composition and structure found in our study, biomimetic coralline alga might become an environmentally friendly antifouling material against the attachment of soft foulants.

• Journal of Plant Biology
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• v.1 no.2
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• pp.7-15
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• 1958

1. The author investigated on the flora of Yongzong island especially on the point of view of the sociological studies. 2. The author could distinguish kinds of the plant formtion in this island(1). Seaside plant formation (2). Mountain plant formation. 3. The seaside plant formation could be subdivided as follows: (1). Association of Triglochin maritimum LINNE. (2). Association of Suaeda japonica MAKINO. (3). Association of Scirpus triqueter LINNE. (4) Association of Phragmites prosturatus MAKINO. (5). Association of Zoysia japonica STEUDEL. (6). Association of Zoysia sinica HANCE var. tenuis NAKAI ex YAMAMOTO. (7). Assocation of Carex pumila THUNBERG. (8). Association of Rosa rugosa THUNBERG. (9). Association of Pinus Thunbergii PARLATORE. (1), (2), (3), (4), (5) associations can grow under the high tide, so when the high tide comes they soaked in the sea water. 4. The mountain plant formation can be subdivided as follows: (1). Association of Quercus aliena BLUME and Platycarya strobilacea STEBOLD & ZUCCARINI. (2). Association of Quercus acutissima CARRUTHERS and Platycaryastrobilacea SIEBOLD & ZUCCARINI. (3). Association of Robinia Pseudoeacia LINNE and Platycarya strobilacea SIEBOLD & ZUCCARINI. 5. The plants at the abandoned mine regions are replacing by the association of Robinia Pseudacacia LINNE and Platycarya strobilacea SIEBOLD & ZUCCARINI. 6. Generally I can find that soil which is developed by the seaside plant formation under sea water (when high tide comes, they soaked in the sea water) is over the pH 8.0, so it shows strong alkaline. But the sandy soil behind the tide line shows near the neutral in spite of the same seaside plant formation. In the case of mountain plant formation the soil where they developed show near the pH 6.0, so it is acidity.