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Differences in liver microRNA profiling in pigs with low and high feed efficiency

  • Miao, Yuanxin;Fu, Chuanke;Liao, Mingxing;Fang, Fang
    • Journal of Animal Science and Technology
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    • v.64 no.2
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    • pp.312-329
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    • 2022
  • Feed cost is the main factor affecting the economic benefits of pig industry. Improving the feed efficiency (FE) can reduce the feed cost and improve the economic benefits of pig breeding enterprises. Liver is a complex metabolic organ which affects the distribution of nutrients and regulates the efficiency of energy conversion from nutrients to muscle or fat, thereby affecting feed efficiency. MicroRNAs (miRNAs) are small non-coding RNAs that can regulate feed efficiency through the modulation of gene expression at the post-transcriptional level. In this study, we analyzed miRNA profiling of liver tissues in High-FE and Low-FE pigs for the purpose of identifying key miRNAs related to feed efficiency. A total 212~221 annotated porcine miRNAs and 136~281 novel miRNAs were identified in the pig liver. Among them, 188 annotated miRNAs were co-expressed in High-FE and Low-FE pigs. The 14 miRNAs were significantly differentially expressed (DE) in the livers of high-FE pigs and low-FE pigs, of which 5 were downregulated and 9 were upregulated. Kyoto Encyclopedia of Genes and Genomes analysis of liver DE miRNAs in high-FE pigs and low-FE pigs indicated that the target genes of DE miRNAs were significantly enriched in insulin signaling pathway, Gonadotropin-releasing hormone signaling pathway, and mammalian target of rapamycin signaling pathway. To verify the reliability of sequencing results, 5 DE miRNAs were randomly selected for quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR results of miRNAs were confirmed to be consistent with sequencing data. DE miRNA data indicated that liver-specific miRNAs synergistically acted with mRNAs to improve feed efficiency. The liver miRNAs expression analysis revealed the metabolic pathways by which the liver miRNAs regulate pig feed efficiency.

A Study on the Optimal Design of Ti-6Al-4V Lattice Structure Manufactured by Laser Powder Bed Fusion Process (Laser Powder Bed Fusion 공정으로 제조된 Ti-6Al-4V 격자 구조물의 최적 설계 기법 연구)

  • Ji-Yoon Kim;Jeongmin Woo;Yongho Sohn;Jeong Ho Kim;Kee-Ahn Lee
    • Journal of Powder Materials
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    • v.30 no.2
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    • pp.146-155
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    • 2023
  • The Ti-6Al-4V lattice structure is widely used in the aerospace industry owing to its high specific strength, specific stiffness, and energy absorption. The quality, performance, and surface roughness of the additively manufactured parts are significantly dependent on various process parameters. Therefore, it is important to study process parameter optimization for relative density and surface roughness control. Here, the part density and surface roughness are examined according to the hatching space, laser power, and scan rotation during laser-powder bed fusion (LPBF), and the optimal process parameters for LPBF are investigated. It has high density and low surface roughness in the specific process parameter ranges of hatching space (0.06-0.12 mm), laser power (225-325 W), and scan rotation (15°). In addition, to investigate the compressive behavior of the lattice structure, a finite element analysis is performed based on the homogenization method. Finite element analysis using the homogenization method indicates that the number of elements decreases from 437,710 to 27 and the analysis time decreases from 3,360 to 9 s. In addition, to verify the reliability of this method, stress-strain data from the compression test and analysis are compared.

Conceptual design of a dual drum-controlled space molten salt reactor (D2 -SMSR): Neutron physics and thermal hydraulics

  • Yongnian Song;Nailiang Zhuang;Hangbin Zhao;Chen Ji;Haoyue Deng;Xiaobin Tang
    • Nuclear Engineering and Technology
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    • v.55 no.6
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    • pp.2315-2324
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    • 2023
  • Space nuclear reactors are becoming popular in deep space exploration owing to their advantages of high-power density and stability. Following the fourth-generation nuclear reactor technology, a conceptual design of the dual drum-controlled space molten salt reactor (D2-SMSR) is proposed. The reactor concept uses molten salt as fuel and heat pipes for cooling. A new reactivity control strategy that combines control drums and safety drums was adopted. Critical physical characteristics such as neutron energy spectrum, neutron flux distribution, power distribution and burnup depth were calculated. Flow and heat transfer characteristics such as natural convection, velocity and temperature distribution of the D2-SMSR under low gravity conditions were analyzed. The reactivity control effect of the dual-drums strategy was evaluated. Results showed that the D2-SMSR with a fast spectrum could operate for 10 years at the full power of 40 kWth. The D2-SMSR has a high heat transfer coefficient between molten salt and heat pipe, which means that the core has a good heat-exchange performance. The new reactivity control strategy can achieve shutdown with one safety drum or three control drums, ensuring high-security standards. The present study can provide a theoretical reference for the design of space nuclear reactors.

Study on the Mechanical Properties of PET Fiber and the Adiabatic Properties of PET Fabrics by their Adiabatic Material Contents (단열성 재료 함량에 따른 PET 원사의 기계적 물성 및 직물의 단열성에 관한 연구)

  • Tae Yoon Kim;Sun Min Kwon;See Hyeon Chae;Ye Dam Jeong;Hyun Je Cho;Ik Sung Choi;Jongwon Kim
    • Textile Coloration and Finishing
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    • v.35 no.2
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    • pp.128-136
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    • 2023
  • Recently, the automobile industry is developing as the demand for automo- biles increases due to industrial development and population growth. In addition, many studies are being conducted to reduce heat loss inside the automobiles in order to save energy inside the automobiles due to environmental regulations. In this study, alumina, nanosilicon, and aerogel, which are adiabatic materials, were filled in PET to manufacture yarn, identify physical and mechanical properties, and weave into fabric to confirm adiabatic performance. As the content of the adiabatic material increased, the tensile strength of the fibers filled with alumina and nanosilicon decreased greatly, and the adiabatic property slightly increased. The tensile strength of fibers filled with the aerogel decreased slightly, but the adiabatic properties were greatly increased. Therefore, it is considered to be due to the large volume fraction in the PET yarn due to the low density of the aerogel.

Effect of oxidants and additives on the polishing performance in tungsten CMP slurry (텅스텐 CMP 연마액에서 산화제와 첨가제가 연마 성능에 미치는 영향)

  • Lee, Jae Seok;Choi, Beom Suk
    • Analytical Science and Technology
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    • v.19 no.5
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    • pp.394-399
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    • 2006
  • The polishing performance and the relationships of electrochemistry depending upon oxidizers and additives in the tungsten CMP slurry used in semiconductor industry were investigated. Hydrogen peroxide, ferric nitrate and potassium iodate were used as oxidizers and they showed different oxidation reactions on tungsten film depending on the kind of oxidizers and pH of slurry. The differences influenced the polishing performance. Etching reaction was predominated in the hydrogen peroxide. However, passivation reaction was prevailed in ferric nitrate and potassium iodate. TMAH and KOH raised the potential energy and removal rate of tungsten, and improved a dispersion characteristic of slurry by increasing absolute value of zeta potential. Addition of 100 ppm of poly(acrylic acid) of M.W. 250,000 improved dispersion ability.

Analysis of Research Trends of Explosion Accidents Using Co-Occurrence Keyword Analysis (동시출현 핵심단어 분석을 활용한 폭발사고 연구 동향 분석)

  • Youngwoo Lee;Minju Kim;Jeewon Lee;Wusung An;Sangki, Kwon
    • Explosives and Blasting
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    • v.42 no.2
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    • pp.12-28
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    • 2024
  • Explosion involving rapid energy diffusion are causing enormous human and economic damage. Due to the advancement of the industry, various and widespread explosion accidents are occurring worldwise, and to prevent such explosion accidents, accurate cause analysis should be the basis. Research analysis related to worldwise explosion accidents was carried out in a limited range for some accidents. By conducting bibliometric analysis of keywords on all the papers published in international journals, this study attempted to derive the overall research trend by period and the latest fields in which future researchers may be interested. As a result of the study of keywords, the number of papers was generally small and the number of overall key words was small from 2005 to 2014, but numerical simulation and artificial intelligence have been used for the analysis of explosion accident cases since 2015, and various studies such as lithium-ion battery and mixed gas, which are the latest research fields, are currently being actively conducted.

Enhanced Biomass Productivity of Freshwater microalga, Parachlorella kessleri for Fixation of Atmospheric CO2 Using Optimal Culture Conditions (최적 배양 조건을 이용한 CO2 제거 목적의 담수 미세조류 Parachlorella kessleri의 바이오매스 생산성 향상)

  • Z-Hun Kim;Sun Woo Hong;Jinu Kim;Byungrak Son;Mi-Kyung Kim;Yong Hwan Kim;Jin Hyun Seol;Su-Hwan Cheon
    • Journal of Marine Bioscience and Biotechnology
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    • v.16 no.1
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    • pp.36-44
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    • 2024
  • This study attempted to improve the growth of the freshwater microalgae, Parachlorella kessleri, through the sequential optimization of culture conditions. This attempt aimed to enhance the microalgae's ability to fixate atmospheric CO2. Culture temperature and light intensity appropriate for microalgal growth were scanned using a high-throughput photobioreactor system. The supplied air flow rate varied from 0.05 to 0.3 vvm, and its effect on the growth rate of P. kessleri was determined. Next, sodium phosphate buffer was added to the culture medium (BG11) to enhance CO2 fixation by increasing the availability of CO2(HCO3-) in the culture medium. The results indicated that optimal culture temperature and light intensity were 20℃-25℃ and 300 μE/m2/s, respectively. Growth rates of P. kessleri under various air flow rates highly depended on the increase of the culture's flow rate and pH which determines CO2 availability. Adding sodium phosphate buffer to BG11 to maintain a constant neutral pH (7.0) improved microalgal growth compared to control conditions (BG11 without sodium phosphate). These results indicate that the CO2 fixation rate in the air could be enhanced via the sequential optimization of microalgal culture conditions.

Fatty Acid Profile and Thermal Behavior of Fat-Rich Edible Insect Oils Compared to Commonly Consumed Animal and Plant Oils

  • Kasidate Chantakun;Tanyamon Petcharat;Saowakon Wattanachant;Muhammad Shahrim Bin Ab Karim;Pensiri Kaewthong
    • Food Science of Animal Resources
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    • v.44 no.4
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    • pp.790-804
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    • 2024
  • This study compared the physicochemical properties of edible insect oils from silkworm (Bombyx mori) pupa (SP), sago palm weevil (Rhynchophorus ferrugineus) larva (PW), and bamboo caterpillar (Omphisa fuscidentalis; BC) to oils from chicken skin (CK), beef back fat (BF), pork back fat (PF), salmon belly (SB), sea bass belly (BB), coconut (C), and peanut (P). The fatty acid profiles and thermal behaviors (crystallization and melting) of the extracted oils were evaluated. PW and BC oils had more saturated fatty acids (SFAs) than CK, PF, SB, BB, and P oils. SP oil had equivalent SFA content to CK and BB oils. Insect oils exhibited similar monounsaturated fatty acid concentrations in all samples, except C oils. PW and BC oils exhibited a higher content of palmitoleic acid than the other oils. SP oils contained polyunsaturated fatty acids similar to those in SB and BB oils, which were higher than those in PW, BC, CK, BF, and PF oils. SP oil also exhibited the highest concentration of α-linolenic acid (C18:3 n-3). Arachidonic acid (0.01-0.02 g/100 g) in all insect oils was lower level compared to CK, BF, PF, SB, and BB oils. SP oil (0.03 g/100 g) exhibited a slightly higher level of eicosapentaenoic acid compared to PW (0.01 g/100 g) and BC (0.01 g/100 g) oils. The insect oils were liquid at ambient temperature, solid below -15℃, and required less energy (∆Hm-max) for melting than other samples. This study indicated that insects, particularly SP, could serve as an alternative source of fat to meet its growing demand.

Structural Analysis of Composite Wind Blade Using Finite Element Technique (유한요소기법을 이용한 복합재 풍력 블레이드 구조해석)

  • Unseong Kim;Kyeongryeol Park;Seongmin Kang;Yong Seok Choi;Kyungeun Jeong;Soomin Lee;Kyungjun Lee
    • Tribology and Lubricants
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    • v.40 no.4
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    • pp.133-138
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    • 2024
  • This study evaluates the structural safety of wind turbine blades, analyzes the behavior of composite laminate structures with and without defects, and assesses surface erosion wear. The NREL 5 MW standard is applied to assign accurate composite material properties to each blade section. Modeling and analysis of the wind turbine blades reveal stable behavior under individual load conditions (gravity, motor speed, wind speed), with the web bearing most of the load. Surface erosion wear analysis in which microparticle impacts are simulated on the blade coating shows a maximum stress and maximum displacement of 14 MPa and 0.02 mm, respectively, indicating good initial durability, but suggest potential long-term performance issues due to cumulative effects. The study examines defect effects on composite laminate structures to compare the stress distribution, strain, and stiffness characteristics between normal and cracked states. Although normal conditions exhibit stable behavior, crack defects lead to fiber breakage, high-stress concentration in the vulnerable resin layer, and decreased rigidity. This demonstrates that local defects can compromise the safety of the entire structure. The study utilizes finite element analysis to simulate various load scenarios and defect conditions. Results show that even minor defects can significantly alter stress distributions and potentially lead to catastrophic failure if left unaddressed. These findings provide valuable insights for wind turbine blade safety evaluations, surface protection strategies, and composite structure health management. The methodology and results can inform the design improvements, maintenance strategies, and defect detection techniques of the wind energy industry.

Pressure Drop Predictions Using Multiple Regression Model in Pulse Jet Type Bag Filter Without Venturi (다중회귀모형을 이용한 벤츄리가 없는 충격기류식 여과집진장치 압력손실 예측)

  • Suh, Jeong-Min;Park, Jeong-Ho;Cho, Jae-Hwan;Jin, Kyung-Ho;Jung, Moon-Sub;Yi, Pyong-In;Hong, Sung-Chul;Sivakumar, S.;Choi, Kum-Chan
    • Journal of Environmental Science International
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    • v.23 no.12
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    • pp.2045-2056
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    • 2014
  • In this study, pressure drop was measured in the pulse jet bag filter without venturi on which 16 numbers of filter bags (Ø$140{\times}850{\ell}$) are installed according to operation condition(filtration velocity, inlet dust concentration, pulse pressure, and pulse interval) using coke dust from steel mill. The obtained 180 pressure drop test data were used to predict pressure drop with multiple regression model so that pressure drop data can be used for effective operation condition and as basic data for economical design. The prediction results showed that when filtration velocity was increased by 1%, pressure drop was increased by 2.2% which indicated that filtration velocity among operation condition was attributed on the pressure drop the most. Pressure was dropped by 1.53% when pulse pressure was increased by 1% which also confirmed that pulse pressure was the major factor affecting on the pressure drop next to filtration velocity. Meanwhile, pressure drops were found increased by 0.3% and 0.37%, respectively when inlet dust concentration and pulse interval were increased by 1% implying that the effects of inlet dust concentration and pulse interval were less as compared with those changes of filtration velocity and pulse pressure. Therefore, the larger effect on the pressure drop the pulse jet bag filter was found in the order of filtration velocity($V_f$), pulse pressure($P_p$), inlet dust concentration($C_i$), pulse interval($P_i$). Also, the prediction result of filtration velocity, inlet dust concentration, pulse pressure, and pulse interval which showed the largest effect on the pressure drop indicated that stable operation can be executed with filtration velocity less than 1.5 m/min and inlet dust concentration less than $4g/m^3$. However, it was regarded that pulse pressure and pulse interval need to be adjusted when inlet dust concentration is higher than $4g/m^3$. When filtration velocity and pulse pressure were examined, operation was possible regardless of changes in pulse pressure if filtration velocity was at 1.5 m/min. If filtration velocity was increased to 2 m/min. operation would be possible only when pulse pressure was set at higher than $5.8kgf/cm^2$. Also, the prediction result of pressure drop with filtration velocity and pulse interval showed that operation with pulse interval less than 50 sec. should be carried out under filtration velocity at 1.5 m/min. While, pulse interval should be set at lower than 11 sec. if filtration velocity was set at 2 m/min. Under the conditions of filtration velocity lower than 1 m/min and high pulse pressure higher than $7kgf/cm^2$, though pressure drop would be less, in this case, economic feasibility would be low due to increased in installation and operation cost since scale of dust collection equipment becomes larger and life of filtration bag becomes shortened due to high pulse pressure.