• Korean Journal of Agricultural Science
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• v.38 no.2
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• pp.269-275
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• 2011

The objective of this study was to develop a rapid and reliable PCR method for sexing of morula or blastocyst stage bovine embryo. BOV97M and bovine 1.715 satellite DNA sequences were selected for amplification of male and bovine specific DNA, respectively. But the unbalanced number of copies of these two repetitive sequences required some modification of PCR method. Karyotyping of blastomeres were carried for the confirmation of sex determination in bovine embryos. The coincidence rate of sex between biopsied-single blastomere and matched blastocyst was 80.0%. When in vivo- and in vitro- derived embryos were compared, 61.8% and 56.7% were male in in vitro- and in vivo-derived embryos, respectively. In vivo-derived embryos showed better hatching rate than in vitro-derived embryos following biopsy of blastomeres. In conclusion, rapid and effective PCR could be applied to sexing of bovine preimplantation embryos using single blastomere. The sensitivity of this assay may eliminate the need for biopsy of more than one nucleated blastomere and reduce trauma to the embryos derived from biopsy procedure.

• Nuclear Engineering and Technology
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• v.32 no.4
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• pp.350-360
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• 2000

In Korea, the dose limits are reduced and are set at the ICRP-60 iimits. However, derived limits tabulated as MPC in air and water are still specified in Notice No.98-12. There are some discrepancies between the primary dose limits and MPCs in air and water. Therefore, in order to accept ICRP-60 recommendations fully, derived limits such as ALI, DAC, ECL for radiological protection against ionizing radiation based on ICRP-60 recommendations were calculated using modified methods of those of 10 CFR part 20, dose limits and committed effective dose coefficients of the Basic Safety Standards of the IAEA. The derived limits in this study were also compared with those prescribed in 10 CFR part 20 as well as MPCs of Notice No. 98-12 in order to analyze the impact of implementing derived limits on nuclear facilities. ECLs in air and water for the control of radioactive discharge into the environment in this study are shown to have lower values (i.e. more conservative), for most part, than those in Notice No. 98-12. Especially, for uranium elements, ECLs in water are approximately a magnitude in the order of two lower than those in Notice No.98-12.

• Preventive Nutrition and Food Science
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• v.15 no.4
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• pp.297-303
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• 2010

The present study compared the Maillard reaction products (MRPs) derived from aqueous and ethanolic fructoseglycine and its oligomer (dimer and trimer) solutions. The pH was lower in glycine (G) than in diglycine (DG) and triglycine (TG) in both aqueous and ethanolic solutions, but the pH difference between the DG and TG was not significant. MRPs derived from the DG had a greater absorbance at 294 and 420 nm in ethanolic solution than in an aqueous solution. In particular, the loss of sugar was higher in ethanolic solution than in aqueous solution. Enolization of fructose was observed in both aqueous and ethanolic MRP solutions; however, enolization was not observed for the G in aqueous MRP solutions. The glycine oligomer content in ethanolic MRP solutions remained higher than that in aqueous MRP solutions. Furthermore, neither diglycine nor triglycine were detected in the G aqueous or ethanolic MRP solutions, while triglycine was detected in both the DG aqueous and ethanolic MRP solutions. Absorption in the ultraviolet-visible (UV-Vis) spectra was higher with MRPs derived from the ethanolic solution than with those derived from the aqueous solution. MRPs derived from the DG in an ethanolic solution showed the highest absorption intensity.

• Biomolecules & Therapeutics
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• v.2 no.2
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• pp.120-125
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• 1994

We obtained the total protein antibodies of Saccharomyces cerevisiae KCTC 1720 and Escherichia coli K-12 from the rabbit and the guinea pig to determine the host-derived proteins which may be remained in biotechnological products. The protein concentration of rabbit antibodies was 4.05 mg/mι in the case of yeast, 7.14 mg/mι in the case of E. coli and that of guinea pig antibodies was 1.90 mg/mι in the case of yeast, 7.17 mg/mι in the case of E. coli, respectively. To determine remained host-derived proteins in biotechnological products which produced by the hosts, S. cerevisiae or E. coli, we used a sandwich enzyme linked immunosorbent assay method in 96 well microplate. When the method applied to determine the remained host-derived proteins in commercial biotechnological products, it detected less than 3.5 ng/vial in human growth hormone, less than 1 ng/vial in hepatitis B vaccine and interferon-${\gamma}$ and 2~23 ng/vial in interferon-$\alpha$. The method can be used to determine the remained host-derived protein in biotechnological products.

• IMMUNE NETWORK
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• v.22 no.5
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• pp.38.1-38.24
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• 2022

Exosomes, which are well-known nanoscale extracellular vesicles, are multifunctional biomaterials derived from endosomes and perform various functions. The exosome is a critical material in cell-cell communication. In addition, it regulates the pathophysiological conditions of the tumor microenvironment in particular. In the tumor microenvironment, exosomes play a controversial role in supporting or killing cancer by conveying biomaterials derived from parent cells. Innate immunity is a crucial component of the host defense mechanism, as it prevents foreign substances, such as viruses and other microbes and tumorigenesis from invading the body. Early in the tumorigenesis process, the innate immunity explicitly recognizes the tumor via Ags and educates the adaptive immunity to eliminate it. Recent studies have revealed that exosomes regulate immunity in the tumor microenvironment. Tumor-derived exosomes regulate immunity against tumor progression and metastasis. Furthermore, tumor-derived exosomes regulate polarization, differentiation, proliferation, and activation of innate immune cells. Exosomes produced from innate immune cells can inhibit or support tumor progression and metastasis via immune cell activation and direct cancer inhibition. In this study, we investigated current knowledge regarding the communication between tumor-derived exosomes and innate immune cell-derived exosomes (from macrophages, dendritic cells, NK cells, and neutrophils) in the tumor microenvironment. In addition, we discussed the potential development of exosomal immunotherapy using native or engineered exosomes against cancer.

• Journal of the Korean Statistical Society
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• v.24 no.1
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• pp.77-90
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• 1995

The Lagrange multiplier test statistics for seasonal unit roots are derived and the asymptotic distributions of the derived statistics are obtained. The relationship between the derived LM test statistics and the "DHF" type regression t statistics are shown.are shown.

• Bulletin of the Korean Mathematical Society
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• v.54 no.5
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• pp.1803-1825
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• 2017

Let X be a smooth scheme with an action of an algebraic group G. We establish an equivalence of two categories related to the corresponding moment map ${\mu}:T^{\ast}X{\rightarrow}g^{\ast}$ - the derived category of G-equivariant coherent sheaves on the derived fiber ${\mu}^{-1}(0)$ and the derived category of G-equivariant matrix factorizations on $T^{\ast}X{\times}g$ with potential given by ${\mu}$.

• Journal of the Korean Operations Research and Management Science Society
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• v.20 no.2
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• pp.1-10
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• 1995

In this study, a threshold policy is considered for the M/M/2 queueing system with server vacations. The probability generating function for the number of customers present in the system is derived using an embedded Markov chain approach. Then, assuming a linear cost structure, an efficient procedure to find an optimal threshold policy is presented. The Laplace-Stieltjes transofrm for th waiting time of an arbitrary customer under a "FIFO" discipline is also derived.o derived.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2019.05a
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• pp.244-245
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• 2019

Recently, a variety of window cleaning devices have been developed. There are, however, few cases of anticipated problems and countermeasures that may arise after these devices are installed. Therefore, in this study, the emergency condition was derived and the emergency scenario was derived for the rail-mounted cleaning device. Emergency scenarios and countermeasures derived from this study will be used for continuous improvement and supplementation of cleaning devices through prototyping and testing.

• Journal of the Korean Society of Marine Environment & Safety
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• v.3 no.2
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• pp.63-67
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• 1997

In this study, some approximate theoretical solutions about oil spreading under the influence of gravitational and viscous forces have been derived from the viewpoint of energy conservation. The theoretical model which derived newly is in agreement with Toi's one derived from a different hypothesis, and shown to predict well the spreading distance of oil front at an initial step of outflow, but further study is necessary to evaluate the spreading distance after longer time.