• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.6
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• pp.725-729
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• 1999

In order to utilize large size anchor effectively, fermented and powdered anchovy seasoning for extractives was manufactured, Fermented anchovy saesoning was fabricated by adding $10\%$ koji of Aspergillus oryzae and mixing with $5\%$ Laminalia. The optimum fermentation temperature, humidity and time for manufacture of anchovy saesoning were $40^{\circ}C$, RH $80\%$ and 48 hrs, respectively. The amount of total free amino acids in anchovy seasoning with $5\%$ Lansinaria was 6,486.9 mg/100 g, while that of commercial product was 444.4 mg/100 g. The principal taste compounds in anchovy seasoning material were IMP and amino acids such as leucine, glutamic acid, histidine, alanine and valine. Extractive nitrogen and organoleptic quality of the extractives in anchovy seasoning packed in tea bag with air permeability, 100 $m^3/m^2/min$, were better than those of commercial product.

• Journal of the Korean Society of Food Culture
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• v.4 no.3
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• pp.293-300
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• 1989

오늘날의 청주(淸酒)는 SAKE라는 이름으로 일본(日本)을 대표(代表)하는 술로 여겨지고 있으나 사실 일본식(日本式) 청주(淸酒)의 제법(製法)도 결국(結局)은 우리나라에 그 근원을 두고 있다. 오늘날의 청주(淸酒)는 우리의 술 빚는 법이 일본(日本)으로 전래된 후, 일본(日本)에서 잘 다듬어져 다시 우리나라에 상륙(上陸)한 것으로 現在의 청주(淸酒)의 근본도 그 뿌리는 백제를 비롯한 우리나라에 있음은 주지할 만한 사실이다. 그러나 우리 고유의 술빚는 법이 체계적으로 정리되지 못하고 사라져 버린데에는 우리의 전통사상의 일부인 과학, 기술 노동을 천시하는 풍조와 술빚는 일을 특히 여성의 전유물로 여겨 양조법의 정립이 이루어지지 못한 것도 큰 이유라 하겠다. 자가용(自家用)으로만 사용되던 주조(酒造)에서 탈피하여 공업적(工業的)인 규모의 청주생산은 1883년 1월 후꾸다라는 일본인이 부산에 세운 공장을 시초로, 1917년 현 백화양조의 전신이라 할 수 있는 조선주조주식회사 설립으로 본격화되었다. 우리의 전통청주(약주)는 곡류와 누룩을 이용하여 빚는데, 누룩은 밀기울을 잘 다져서 장기간에 걸쳐 제조하며, 누룩에는 당화효소를 분비하는 곰팡이류와 알콜발효를 할 수 있는 효모류가 함께 부착되어 있다. 따라서 호화시킨 곡류와 누룩을 함께 넣어서 술을 빚게되면 당화와 발효가 동시에 일어나게 된다. 일본식 청주도 Koji라 불리는 국(麴)을 제조하는 것을 달리할 뿐 우리 전통청주와 제법의 맥락은 같다. Koji는 찐쌀에 곰팡이류를 인위적으로 접종하여 배양한 것으로, 결국 당과용 효소를 얻는다는 것은 누룩과 같은 원리이나 곰팡이균을 분리하여 접종하는 것이 다르다. 한국의 청주판매 현황을 보면 80년대 초반 급격한 감소추세를 보였으나, 업계가 적극적인 판촉활동을 벌임과 동시에 새로운 TYPE의 냉청주(冷淸酒)를 개발하여 여름철이 비수기였던 시장 상황을 완전히 깨뜨려 사계절의 주류로 만드므로서 뚜렷한 회복, 증가추세로 바꾸었다. 앞으로 좀더 잘 다듬어지고 현대 이미지에 맞는 청주가 지속적으로 등장하여야 하며, 결국 이러한 민족고유의 맛이 가미된 청주 TYPE의 주류가 우리나라를 대표하는 국주로까지 발전할 수 있으리라 확신한다.

• Preventive Nutrition and Food Science
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• v.21 no.3
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• pp.221-226
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• 2016

A new type of doenjang was manufactured by mixing soaked soybean, koji (Rhizopus oryzae), cheonggukjang (Bacillus amyloliquefaciens MJ1-4 and B. amyloliquefaciens EMD17), and Pichia farinosa SY80 as a yeast, salt, and water, followed by fermentation with koji that was made by fermenting whole wheat with R. oryzae. The mixed culture doenjang was designed to have a more palatable flavor and stronger biological activities than the conventional product. The extract of mixed culture doenjang showed higher antioxidant activity than the commercial doenjang as evaluated by the ferric reducing antioxidant power assay although it was not significantly different from the commercial product in 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activities. Further, the mixed culture doenjang reduced intracellular reactive oxygen species levels and protected cells from glutamate-induced cytotoxicity more efficiently in human hippocampal HT22 neuroblastoma cells than the commercial doenjang. In conclusion, a newly-developed mixed culture doenjang had a strong antioxidant activity in vitro and cultured cell model systems, exhibited a potential to prevent oxidative stress-associated disorders although animal and clinical studies are needed to confirm its in vivo efficacy.

• Journal of the East Asian Society of Dietary Life
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• v.19 no.1
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• pp.96-104
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• 2009

Three kinds of grape yakju (Kyoho, Campbell and Moru) and grape-free control were fermented with rice using koji of Aspergillus kawachii as a fermenting agent, and their fermentation characteristics were investigated. For the three yakju, reducing sugar content decreased slowly over 2 days fermentation after an initial rapid decrease. In case of Brix, the reducing sugar content increased gradually after increasing rapidly during the first 2 days of fermentation. The Kyoho and Moru groups displayed a gradual increase in reducing sugar content after an initial 2-day decrease, and the sugar content of the Campbell group increased gradually during fermentation. Uniformly, pH increased gradually after an initial 2-day decrease, however total acid increased continuously throughout fermentation. Alcohol content of yakju showed almost no change after increasing until day 6 (Kyoho) of day 4 (others), with the control displaying the highest content. Control $\alpha$-amylase activity decreased gradually after an initial 2-day increase, while that of the three yakju decreased throughout fermentation. Activities of $\beta$-amylase activities paralleled the results of reducing sugar. Concerning total anthocyanin, the Moru and Campbell groups displayed a rapid increase until day 2, a decrease until day 12, and a gradual increase thereafter. While the Kyoho and control groups displayed virtually no change after an initial 2-day increase until day 12, after which a gradual increase also occurred. In concerning sensory evaluation, Moru yakju scored highest for violetness, flavor of grape, and sweet taste, and also displayed a low alcohol content. These attributed Moru yakju the preferred choice.

• Journal of Microbiology and Biotechnology
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• v.27 no.2
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• pp.251-261
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• 2017

Initially, we screened 18 Aspergillus sojae-like strains from Aspergillus spp. isolated from meju (Korean traditional fermented soybean brick) according to their morphological characteristics. Because members of Aspergillus section Flavi are often incorrectly identified because of their phylogenetic similarity, we re-identified these strains at the morphological and molecular genetic levels. Fourteen strains were finally identified as A. sojae. The isolates produced protease and ${\alpha}-amylase$ with ranges of 2.66-10.64 and 21.53-106.73 unit/g-initial dry substrate (U/g-IDS), respectively, which were equivalent to those of the koji (starter mold) strains employed to produce Japanese soy sauce. Among the isolates and Japanese koji strains, strains SMF 127 and SMF 131 had the highest leucine aminopeptidase (LAP) activities at 6.00 and 6.06 U/g-IDS, respectively. LAP plays an important role in flavor development because of the production of low-molecular-weight peptides that affect the taste and decrease bitterness. SMF 127 and SMF 131 appeared to be non-aflatoxigenic because of a termination point mutation in aflR and the lack of the polyketide synthase gene found in other A. sojae strains. In addition, SMF 127 and SMF 131 were not cyclopiazonic acid (CPA) producers because of the deletion of maoA, dmaT, and pks/nrps, which are involved in CPA biosynthesis. Therefore, A. sojae strains such as SMF 127 and SMF 131, which have high protease and LAP activities and are free of safety issues, can be considered good starters for soybean fermentations, such as in the production of the Korean fermented soybean products meju, doenjang, and ganjang.

• Korean Journal of Food Science and Technology
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• v.4 no.3
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• pp.182-186
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• 1972

The possibility of substituting corn or barley for the wheat, one of the raw materials for soy sauce, was studied by measuring the amylase and proteolytic activities of koji. Also optimum conditions of koji making were determined. It was found that substitution of up to 60% of wheat content (of the total bean and wheat content) with corn, yielded good quality of soy sauce. It is also found that barley can substitute 70% of wheat content (35% of the total content).

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.3
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• pp.346-351
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• 2009

This study was carried out to determine the rheological properties of bread doughs containing flour-ferments prepared with seed mash obtained by Koji incubation, yeast, and lactic acid bacteria, using farinograph, extensograph, amylograph, and large scale-dough mixer. According to farinograph, the addition of the flour-ferments did not influence the water-absorption rate of doughs, regardless of the kinds of flour-ferments, however, it increased development time and decreased stability of doughs. According to extensograph, both dough resistance and resistance-to-extensibility ratio increased with the addition of flour-ferments. Especially the dough containing the flour-ferments prepared with seed mash, S. cerervisiae, and L. brevis showed the highest resistance-to-extensibility ratio. According to amylograph, although the doughs containing the flour-ferments did not show the differences in gelatinization temperature and temperature at maximum viscosity with the control which does not contain the flour-ferments, they showed lower maximum viscosity than the control. They also showed lower development value and faster development time.

• Food Science and Preservation
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• v.10 no.4
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• pp.522-526
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• 2003

The quality properties of vinegar produced by Takju mash added muskmelon for the practical use of inferior muskmelon was analyzed. While the value of L was shown high at the muskmelon 30% added(A) and the value of a was shown high at the wheat koji added (B) the value of b was decreased at all sections. As the organic acids, oxalic, tartaric, malic, lactic, acetic citric and succinic acid were detected and there was no difference for the acetic acid content. There was difference by raw materials for free amino acid and tyrosine (35.70 mg%) was high at (C) wheat koji 15% added. As a result, the quality of Takju mash vinegar added muskmelon was generally superior and there was no difference by raw materials.

• Publications of The Korean Astronomical Society
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• v.30 no.2
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• pp.679-682
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• 2015

The Hiroshima Astrophysical Science Center (HASC) was founded in 2004 at Hiroshima University, Japan. The main mission of this institute is the observational study of various transient objects including gamma-ray bursts, supernovae, novae, cataclysmic variables, and active galactic nuclei by means of multi-wavelength observations. HASC consists of three divisions; the optical-infrared astronomy division, high-energy astronomy division, and theoretical astronomy division. HASC is operating the 1.5m optical-infrared telescope Kanata, which is dedicated to follow-up and monitoring observations of transient objects. The high-energy division is the key operation center for the Fermi gamma-ray space telescope. HASC and the high-energy astronomy group in the department of physical science at Hiroshima University are closely collaborating with each other to promote multi-wavelength time-domain astronomy. We report the recent activities of HASC and some science topics pursued by this multi-wavelength collaboration.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.9
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• pp.1461-1466
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• 2013

In this study, we ascertained the characteristics of crude protease extracted from fruits (fig, kiwifruit and pineapple) as well as traditional Korean fermentation starters (bio nuruk, traditional nuruk, meju and rice koji) to determine their suitability for industrial application. Crude protease extracted from traditional Korean fermentation starters was found to have a higher optimum temperature ($70^{\circ}C$) and salt concentration (1~3%) but a lower optimum pH (3~6) value compared to the corresponding values for the protease extracted from fruits. On comparison, the total activities of protease per gram unit follow the order: bio nuruk> rice koji> traditional nuruk> fig> pineapple> meju> kiwifruit. Based on our results, we conclude that protease extracted from traditional Korean fermentation starters has potential for application in food industry, for example, as a meat tenderizer for sausage manufacturing and as a protease for cheese production.