• Journal of Microbiology and Biotechnology
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• v.28 no.3
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• pp.439-447
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• 2018

The aromatic compound p-hydroxybenzoate (PHBA) is an important material with multiple applications, including as a building block of liquid crystal polymers in chemical industries. The cytochrome P450 (CYP) enzymes are beneficial monooxygenases for the synthesis of chemicals, and CYP53A15 from fungus Cochliobolus lunatus is capable of executing the hydroxylation from benzoate to PHBA. Here, we constructed a system for the bioconversion of benzoate to PHBA in Escherichia coli cells coexpressing CYP53A15 and human NADPH-P450 oxidoreductase (CPR) genes as a redox partner. For suitable coexpression of CYP53A15 and CPR, we originally constructed five plasmids in which we replaced the N-terminal transmembrane region of CYP53A15 with a portion of the N-terminus of various mammalian P450s. PHBA productivity was the greatest when CYP53A15 expression was induced at $20^{\circ}C$ in $2{\times}YT$ medium in host E. coli strain ${\Delta}gcvR$ transformed with an N-terminal transmembrane region of rabbit CYP2C3. By optimizing each reaction condition (reaction temperature, substrate concentration, reaction time, and E. coli cell concentration), we achieved 90% whole-cell conversion of benzoate. Our data demonstrate that the described novel E. coli bioconversion system is a more efficient tool for PHBA production from benzoate than the previously described yeast system.

• 한국전산유체공학회:학술대회논문집
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• 2003.10a
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• pp.96-97
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• 2003

• Journal of Advanced Marine Engineering and Technology
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• v.30 no.4
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• pp.474-482
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• 2006

Generally almost all fatigue crack growth is affected by mode I. For this reason a study on mode I has concentrated in the field of fracture mechanics. However the fatigue crack initiation and growth in machines and structures usually occur in mixed mode loading. If there is any relationship between the cause of fracture in mixed mode loading and fracture surface, fracture surface pattern will be the main mean explaining reasons of fatigue fracture and obtaining further information about fracture process. In this paper low point shear-fatigue test with Aluminum alloy hi 5083-O is carried out from this prospect and then the mixed mode distribution of fracture surface is examined from the result after identifying the generation of fatigue crack surface pattern. It was found from the experimental results that the fatigue crack surface pattern and the fatigue crack shear direction are remarkably consistent. Furthermore It is possible that the analysis of distribution of mixed mode through the fatigue crack surface pattern.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.10
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• pp.1394-1398
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• 2006

The complete mtDNA D-loop regions of Japanese and Korean cattle were analyzed for their mtDNA variations and genetic relationships. Sequencing the 30 Higo substrain and 30 Tosa substrain of Japanese Brown, respectively 12 and 17 distinct Bos haplotypes were identified from 77 polymorphic nucleotide sites. In order to focus on the relationships among Japanese and Korean cattle, two types of phylogenetic tree were constructed using individual sequences; first, a neighbor-joining tree with all sequences and second, reduced median networks within each Japanese and Korean cattle group. The trees revealed that two major mtDNA haplotype groups, T3 and T4, were represented in Japanese and Korean cattle. The T4 haplogroup predominated in Japanese Black and Japanese Brown cattle (frequency of 43.3-66.7%), while the T3 haplogroup was predominant (83.3%) and T4 was represented only twice in the Korean cattle. The results suggested that the mitochondrial origins of Japanese Brown were Japanese ancient cattle as well as Japanese Black in despite of the considerable introgression of Korean and European cattle into Japanese Brown.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.1
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• pp.165-171
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• 1992

The developmental process of the epididymis was investigated in Meishan boars from 1 to 364 days of age. Epididymal weight increased rapidly between 45 and 150-180 days of age. The diameter and epithelial area of the epididymal ducts greatly increased up to 105-120 days of age. At 1 day of age, the central and distal cauda already had a pseudostratified epithelium surrounded by smooth muscle. At 60-75 days of age, the central and distal caput, corpus, and proximal cauda revealed a well-developed structure of the epithelium. The proximal caput showed a tall, irregular and vacuolar epithelium at 105-120 days of age. PAS-positive contents in the lumen of the caput, corpus and cauda epididymides were first detected at 60-75, 45-60 and 1-30 days of age, respectively. Moreover, in the central and distal caput, PAS-positive granules appeared at 60-75 days of age, and increased until 105-120 days. These results suggest that the epididymis develops completely by approximately 120 days of age, though its weight increases rapidly up to 150-180 days. Thus, it appears that development of the epididymis occurs at an earlier age in Meishan boars than in European and American breeds.

• Journal of Korean Neurosurgical Society
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• v.59 no.4
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• pp.400-404
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• 2016

We report a case of acute ischemic stroke involving both the anterior and posterior circulation associated with a persistent primitive trigeminal artery (PPTA), treated by endovascular revascularization for acute basilar artery (BA) occlusion via the PPTA. An otherwise healthy 67-year-old man experienced sudden loss of consciousness and quadriplegia. Magnetic resonance imaging showed an extensive acute infarction in the right cerebral hemisphere, and magnetic resonance angiography showed occlusion of the right middle cerebral artery (MCA) and BA. Because the volume of infarction in the territory of the right MCA was extensive, we judged the use of intravenous tissue plasminogen activator to be contraindicated. Cerebral angiography revealed hypoplasia of both vertebral arteries and the presence of a PPTA from the right internal carotid artery. A microcatheter was introduced into the BA via the PPTA and revascularization was successfully performed using a Merci Retriever with adjuvant low-dose intraarterial urokinase. After treatment, his consciousness level and right motor weakness improved. Although persistent carotid-vertebrobasilar anastomoses such as a PPTA are relatively rare vascular anomalies, if the persistent primitive artery is present, it can be an access route for mechanical thrombectomy for acute ischemic stroke.

• Journal of Korean Society of Rural Planning
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• v.19 no.4
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• pp.283-291
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• 2013

In this paper, in order to clarify the effect and characteristics of rural women enterprise in rural areas, AWATAKE Restaurant at Kita Ward, Kobe is taken as a case study, and its management and operation condition, and the effects towards the region are analyzed. As a result, it can be concluded that: 1) according to the local employment and purchase of local food products, the economic effect towards the area shows a returning 73% of the gross sales to the area, 2) lifestyle technology which is empowered by women and food culture which is used as a start in the enterprise activities, are functioning as new workplace to cultivate technologies own by the women, moreover, members have never decreased, and most of them stayed for as long as 10 years, 3) however, the average age of the members are high and decreasing, leaving a new task on how to encourage participation of young members, 4) lastly, rural women enterprise is easy to manage, and also received cooperation from family in the members' working environment.

• Journal of Magnetics
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• v.18 no.2
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• pp.173-177
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• 2013

Cantilever-detected high-frequency electron spin resonance (ESR) is a powerful method of sub-terahertz and terahertz ESR spectroscopy for a tiny magnetic sample at low temperature. In this technique, a small magnetization change associated with ESR transition is detected as deflection of a sample-mounted cantilever. So far, we have succeeded in ESR detection at 370 GHz using a commercial piezoresistive microcantilever. The spin sensitivity was estimated to ${\sim}10^{12}$ spins/gauss. In order to further increase the sensitivity, we adopt a fiber-optic-based detection system using a Fabry-Perot interferometer in place of piezoresistive system. Fabry-Perot cavity is formed between an optical-fiber end and microcantilever surface, and a change in the interference signal, corresponding to the cantilever deflection, is sensitively detected. This system is suitable for low-temperature and high-magnetic-field experiments because of its compact setup and less heat dissipation. In this study, performance of Fabry-Perot interferometer is evaluated, and its application to cantilever-detected ESR measurement is described.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.09c
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• pp.11-17
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• 2010

Numerical analysis was carried out in order to simulate the development of the large deformation that took place on the reinforced earth wall, a part of the Tottori expressway planned to pass Hyogo, Japan. Since this reinforced earth wall had experienced unexpected deformation of the wall during construction, the wall was re-constructed twice. However, the wall deformation showed no sign to cease even at the final stage of the construction. Countermeasures to re-stabilize the wall were demanded. In part I of this paper, it was manifested that subsidence of a 3-meter weak soil due to seepage flow was responsible for the large deformation. A part of concrete panel wall was severely damaged due to extremely large pulling force of geotextile induced by the hammock state. As for the countermeasures, "grouting with slag system" was applied to fill voids of the backfill, and also to prevent further development of settlement in the weak soil layer. "Ground anchor" was also considered to achieve the prescribed factor of safety.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2006.10a
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• pp.49-55
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• 2006

Kinematic GPS provides quite good accuracy of position in cm level. Though K-GPS assures high precision measurement in cm level on the basis of an appreciable distance between a station and an observational point, but it has measurable distance restriction within 20 km from a reference station on land. So it is necessary to make out a simple and low-cost method to obtain accurate positioning information without distance restriction. In this paper, the velocity integration method to get the precise velocity information of ship is explained. Next two experimental results (Zig-zag maneuvering test and Williamson turn) as the ship's maneuvering test and also the experimental results of leaving and entering port as slow speed ship's movement were shown. In these experimental results, ship's course, speed and position are compared with those obtained by kinematic-GPS, velocity integration method and dead reckoning position using Gyro-compass and Doppler-log.