• Journal of Animal Reproduction and Biotechnology
• /
• v.36 no.3
• /
• pp.121-128
• /
• 2021

Increasing the efficiency of HR (homologous recombination) is important for a successful knock-in. Rad51 is mainly involved in homologous recombination and is associated with strand invasion. The HR-related mismatch repair system maintains HR fidelity by heteroduplex rejection and repair. Therefore, the purpose of this study is to control Rad51, which plays a critical role in HR, through UV-induced DNA damage. It is also to confirm the effect on the expression of MMR related genes (Msh2, Msh3, Msh6, Mlh1, Pms2) and HR-related genes closely related to HR through treatment with the MMR inhibitor CdCl₂. The mRNA expression of Rad51 gene was confirmed in both HC11 cells and mouse testes, but the mRNA expression of Dmc1 gene was confirmed only in mouse testes. The protein expression of Rad51 and Dmc1 gene increased in UV-irradiated HC11 cells. After 72 hours of treatment with 1 ㎛ of CdCl₂, the mRNA expression level of Msh3, Pms2, and Rad51 decreased, but the mRNA expression level of Msh6 and Mlh1 increased in HC11 cells. There was no significant difference in Msh2 mRNA expression between CdCl₂ untreated-group and the 72 hours treated group. In conclusion, HR-related gene (Rad51) was increased by UV-induced DNA damage. Treatment of the MMR inhibitor CdCl₂ in HC11 cells decreased the mRNA expression of Rad51.

• Journal of Advanced Marine Engineering and Technology
• /
• v.30 no.4
• /
• pp.455-462
• /
• 2006

Natural gas is a promising alternative fuel to meet strict engine emission regulations in many countries. Natural gas engines can operate at lean burn and stoichiometric burn conditions with different combustion and emission characteristics. In this paper, the fuel economy, emissions, misfire, knock and cycle-to-cycle variations in indicated mean effective pressure of lean burn natural gas engines are highlighted. Stoichiometric burn natural gas engines are briefly reviewed. To keep the output power and torque of natural gas engines comparable to that of gasoline engines, high boosting pressure should be used. High activity catalyst for methane oxidation and lean deNOx system or three way catalyst with precisely control strategies should be developed to meet stringent emission standards.

• Journal of Energy Engineering
• /
• v.19 no.1
• /
• pp.32-38
• /
• 2010

The objective of the research was to study the effects a Miller cycle. The engine was dedicated to natural gas usage by modifying pistons, fuel system and ignition systems. The engine was installed on a dynamometer and attached with various sensors and controllers. Intake valve timing, engine speed, load, injection timing and ignition timing are main parameters. Miller Cycle without supercharging can increase brake thermal efficiency 1.08% and reduce brake specific fuel consumption 4.58%. The injection timing must be synchronous with valve timing, speed and load to control the performances, emissions and knock margin. Throughout these tested speeds, original camshaft is recommended to obtain high volumetric efficiency.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.16 no.2
• /
• pp.128-135
• /
• 2008

EGR(exhaust gas recirculation) is considered as a most effective method to reduce the NOx emissions. But high EGR tolerance is always pursued not only for its advantages of the pumping loss reduction and fuel economy benefit in Gasoline-Hybrid engine. However, the occurrence of excessive cyclic variation with high EGR normally prevents substantial fuel economy improvements from being achieved in practice. Therefore, the optimum EGR rate should be carefully determined in order to achieve low fuel consumption and low exhaust emission. In this study, 2 liters gasoline engine with E-EGR system was used to investigate the effects of EGR on fuel efficiency, combustion stability, engine performance and exhaust emissions. With optimal EGR rates, the fuel consumption was improved by 4%. This improvement was achieved while a reduction in NOx emissions of 75% was accomplished. Increase of EGR gas temperature causes the charge air temperature to affect the knock phenomenon and moreover, the EGR valve lift changes for the same control signal.

• Journal of Energy Engineering
• /
• v.17 no.4
• /
• pp.198-203
• /
• 2008

The objective of the research was to study the effects of Miller cycle in a modified using diesel engine. The engine was dedicated to natural gas usage by modifying pistons, fuel system and ignition systems. The engine was installed on a dynamometer and attached with various sensors and controllers. Intake valve timing, engine speed, load, injection timing and ignition timing are main parameters. The results of engine performances and emissions are present in form of graphs. Miller Cycle without supercharging can increase brake thermal efficiency and reduce brake specific fuel consumption. The injection timing must be synchronous with valve timing, speed and load to control the performances, emissions and knock margin. Throughout these tested speeds, original camshaft is recommended to obtain high volumetric efficiency. Retard ignition timing can reduce $NO_x$ emissions while maintaining high efficiency.

• Journal of Microbiology and Biotechnology
• /
• v.27 no.7
• /
• pp.1276-1280
• /
• 2017

The mcr-1 gene is a new "superbug" gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The mcr-1 gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce mcr-1 gene knockout in Escherichia coli. Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene mcr-1 might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas::mcr targeting specific DNA sequences of the mcr-1 gene into microbial populations.

• Journal of Life Science
• /
• v.19 no.7
• /
• pp.852-858
• /
• 2009

The homeostasis of the pyridine nucleotide pool [NAD(P)H and NAD(P)$^+$] is maintained in Rhodobacter sphaeroides mutant strains defective in the cytochrome bci complex or the cytochrome c oxidases in terms of its concentration and redox state. Aerobic derepression of the puf operon, which is under the control of the PrrBA two-component system, in the CBB3 mutant strain of R. sphaeroides was shown to be not the result of changes in the redox state of the pyridine nucleotides and the ubiquinone/ubiquinol pool. Using the bc$_1$ complex knock-out mutant strain of R. sphaeroides, we clearly demonstrated that the inhibitory effect of cbb$_3$, oxidase on spectral complex formation is not caused indirectly by the redox change of the ubiquinone/ubiquinol pool.

• Development and Reproduction
• /
• v.21 no.2
• /
• pp.157-165
• /
• 2017

One of the reasons to causing blood coagulation in the tissue of xenografted organs was known to incompatibility of the blood coagulation and anti-coagulation regulatory system between TG pigs and primates. Thus, overexpression of human CD73 (hCD73) in the pig endothelial cells is considered as a method to reduce coagulopathy after pig-to-non-human-primate xenotransplantation. This study was performed to produce and breed transgenic pigs expressing hCD73 for the studies immune rejection responses and could provide a successful application of xenotransplantation. The transgenic cells were constructed an hCD73 expression vector under control porcine Icam2 promoter (pIcam2-hCD73) and established donor cell lines expressing hCD73. The numbers of transferred reconstructed embryos were $127{\pm}18.9$. The pregnancy and delivery rate of surrogates were 8/18 (44%) and 3/18 (16%). The total number of delivered cloned pigs were 10 (2 alive, 7 mummy, and 1 died after birth). Among them, three live hCD73-pigs were successfully delivered by Caesarean section, but one was dead after birth. The two hCD73 TG cloned pigs had normal reproductive ability. They mated with wild type (WT) MGH (Massachusetts General Hospital) female sows and produced totally 16 piglets. Among them, 5 piglets were identified as hCD73 TG pigs. In conclusion, we successfully generated the hCD73 transgenic cloned pigs and produced their litters by natural mating. It can be possible to use a mate for the production of multiple transgenic pigs such as ${\alpha}-1,3-galactosyltransferase$ knock-out /hCD46 for xenotransplantation.

• Development and Reproduction
• /
• v.11 no.3
• /
• pp.263-272
• /
• 2007

Contrast to mouse where its in vitro maturation rates are high without specific supplements or presence of the cumulus cells, there are some species, such as porcine, where its in vitro oocyte maturation rates are still very low. This comparative study was conducted to investigate the role of malate dehydrogenase(Mor2) during oocyte maturation by RNAi in the mouse and porcine. The Mor2 double-stranded RNA(dsRNA) was prepared speciesspecifically and microinjected into the cytoplasm of denuded germinal vesicle(GV) oocytes. Oocytes were cultured for 48 h(porcine) and 16 h(mouse) in M199 with 10% porcine follicular fluid, pyruvate, p-FSH, EGF, cystein, and estradiol-$17{\beta}$. We measured changes in oocyte morphology, maturation rates and mRNA levels after Mor2 RNAi. We confirmed gene sequence-specific knock down of Mor2 mRNA in both species after Mor2 RNAi. In contrast to our previous finding that mMor2 RNAi resulted in GV arrest in the mouse, we found that pMor2 RNAi resulted in MI arrest in denuded porcine oocytes(58%), but developed to MII(84.4%) in COCs. To determine whether this difference between mouse and porcine RNAi is due to differences in culture media, we cultured mouse oocytes in the M199 media for 16 h after mMor2 RNAi. Mouse oocytes were developed to MII stage(62%) and there was no statistical difference compared to that of non-injected(76.8%) and buffer-injected(73.3%) control groups. Therefore, we concluded that the mouse and porcine oocytes are having different metabolic systems in relation to malate dehydrogenase for oocyte maturation. This could be a basis for differences in maturation rates in vitro in two species. Further scrutinized studies on the metabolic pathways would led us in finding better culture system to improve oocyte maturation rates in vitro, especially in more challenging species like the porcine.