• 제목/요약/키워드: Km value

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Streptomyces alboniger가 생산(生産)하는 Protease의 특성(特性)에 관(關)한 연구(硏究) (Studies on the Characterization of Protease Produced by Streptomyces alboniger)

  • 김강신;한강완;김형로
    • Applied Biological Chemistry
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    • 제27권3호
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    • pp.174-179
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    • 1984
  • Streptomyces alboniger Hesseltin이 생산(生産)하는 단백질(蛋白質) 가수분해(加水分解) 효소(酵素)의 몇가지 이화학적(理化學的) 성질(性質)을 구명(究明)하여 본 결과(結果), 다음과 같다. 본(本) 효소(酵素)의 작용(作用) 최적(最適) pH와 작용(作用) 최적(最適) 온도(溫度)는 각각 pH 9.0과 $40^{\circ}C$ 부근이었다. 본(本) 효소(酵素)의 안정(安定) pH 범위는 pH $6.0{\sim}10.8$ 부근이었으며, 열처리(熱處理)에 대하여 $40^{\circ}C$ 이하(以下)에서 안정(安定)하였으나 $70^{\circ}C$에서는 10분(分) 처리(處理)로 처음 효소(酵素) 활성(活性)의 70%가 감소하였다. 본(本) 효소(酵素)는 Hammarsten casein과 cytocrom C에 대한 Km치(値)가 각각 $7.1{\mu}M$$333.3{\mu}M$이었다. 효소(酵素)의 활성(活性)은 금속(金屬) 이온에 대하여 $Fe^{+++}>Hg^{++}>Cu^{++}>Pb^{++}>Zn^{++}$의 순서로 저해(沮害)되었고 $Ca^{++}$ 에 의하여 효소(酵素) 활성(活性)이 증가된 반면, $Mg^{++}$$Co^{++}$에 의해서는 별 다른 영향(影響)을 받지 않았다. 또한 효소(酵素)는 EDTA에 의하여 강하게 저해(沮害)되었으며, EDTA에 의해 변성된 효소(酵素)에 $Ca^{++}$을 첨가할 경우 효소(酵素) 활성(活性)이 부활(復活)되었다.

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터널 경계부 휘도수준에 따른 운전자 연령대별 안전수준 비교 (Comparison of Safety Level between Driver's Ages by Threshold Zone Luminance Level of Vehicular Traffic Tunnel)

  • 조원범;정준화;김도경;박원일
    • 한국도로학회논문집
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    • 제17권1호
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    • pp.129-142
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    • 2015
  • PURPOSES : The purpose of this study is to suggest a basis for setting appropriate safety goals specifically related to the threshold zone luminance in a vehicular traffic tunnel. METHODS : In the test, drivers were divided into two groups. One group consisted of all drivers (average drivers) group with an age ratio of drivers holding domestic driver's license and driver group by age to produce threshold zone luminance in the tunnel. The threshold zone luminance produced as a result was used to analyze how it affects the safety level of each driver group and provide a basis for setting an appropriate safety criterion that can be used to determine threshold zone luminance. We used test equipment, test conditions, and ananalysis of threshold zone luminance identical to that reported by ChoandJung(2014) but the values of adaptation luminance in our analys is were expanded to range from100 to $10,000cd/m^2$. RESULTS : Adaptation luminance and threshold zone luminance are found to be related by a quadratic function. The threshold zone luminance needed by older drivers to ensure a certain safety level is significantly higher than that for drivers of other age brackets when adaptation luminance increases. 56% of older drivers are at an increased risk of an accident at the same luminance for which the safety level of average drivers is 75%. The safety level that can be achieved for older drivers increases to above 60% when threshold zone luminance level is set with the goal of attaining a safety level of more than 85% for average drivers. The safety level that can be attained for average drivers is above 90% when the threshold zone luminance is high enough to ensure over 75% in the safety level of older drivers. Results of this study are applicable to highways and others whose designed speed is 100 km/h. CONCLUSIONS : Threshold zone luminance determined on the basis of drivers having average visual ability is of limited value as a performance standard for ensuring the safety of older drivers. Hence, safety level for older drivers should be considered separately from safety levels for drivers with an average ability to avoid risk. Upward adjustment of older drivers' safety level in the process of determining appropriate threshold zone luminance in a vehicular traffic tunnel may bring both tangible and intangible benefit as a result of reducing accidents. However, there is an associated dollar cost arising from installing and operating lights. As a result, the economic impact of these trade-offs should also be considered.

Effect of Transportation at High Ambient Temperatures on Physiological Responses, Carcass and Meat Quality Characteristics in Two Age Groups of Omani Sheep

  • Kadim, I.T.;Mahgoub, O.;AlKindi, A.Y.;Al-Marzooqi, W.;Al-Saqri, N.M.;Almaney, M.;Mahmoud, I.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • 제20권3호
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    • pp.424-431
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    • 2007
  • The aim of this study was to determine the effects of short road transportation in an open truck during hot season on live weight shrink, physiological responses, and carcass and meat quality of Omani sheep at 6 and 12 months of age. Thirty-six male sheep, 18 of each age group, were used. Age groups were assigned randomly to transported and not-transported groups. The transported group was transported to the slaughterhouse the day of slaughter in an open truck covering a distance of approximately 100 km. The average temperature during transportation was $37^{\circ}C$. The not-transported group was kept in a lairage of a commercial slaughterhouse with ad libitum feed and water for 48 h prior to slaughter. Blood samples were collected from sheep before loading and prior to slaughter via jugular venipuncture to assess their physiological response to transport in relation to hormonal levels. Animals were weighed just before loading onto a truck and after transport to assess shrinkage. Muscle ultimate pH, expressed juice, cooking loss percentage, WB-shear force value, sarcomere length and colour L*, a*, b* were measured on samples from longissimus dorsi, biceps femoris and semitendinosus muscles collected at 24 h postmortem at $1-3^{\circ}C$. Live weight shrinkage losses were 1.09 and 1.52 kg for 6 and 12 month transported sheep, respectively. The transported sheep had significantly (p<0.05) higher cortisol, adrenaline, noradrenaline, and dopamine concentration levels prior to slaughter at both ages than the not-transported sheep. Transportation significantly influenced meat quality characteristics of three muscles. Muscle ultimate pH and shear force values were significantly higher, while CIE L*, a*, b*, expressed juice and cooking loss were lower in transported than not-transported sheep. Age had a significant effect on meat quality characteristics of Omani sheep. These results indicated that short-term pre-slaughter transport at high ambient temperatures can cause noticeable changes in physiological and muscle metabolism responses in sheep.

한반도 태풍강우 추출기법 비교 평가 (Comparative Assessment of a Method for Extraction of TC-induced Rainfall Affecting the Korean Peninsula)

  • 손찬영;권현한;김종석;문영일
    • 한국수자원학회논문집
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    • 제47권12호
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    • pp.1187-1198
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    • 2014
  • 서북태평양에서 발생하는 태풍은 돌풍 및 강우를 동반하여 한반도뿐만 아니라 동아시아에 상당한 인적 및 물질피해를 야기함에 따라 이에 대한 수방대책 및 치수정책 수립이 시급한 실정이다. 따라서 본 연구에서는 태풍의 경로 및 규모를 고려한 태풍강우량 추출기법을 제안하고 과거 태풍 감시구역을 이용한 태풍강우 추출기법과 비교하여 적용성을 검토하였다. 태풍규모를 결정하기 위해 본 연구에서는 평균 태풍강우량을 산정하였으며, 산정결과 태풍 규모가 반경 700 km일때 평균 태풍강우량이 최대로 나타났다. 또한, 본 연구에서 제안하는 태풍의 경로 및 규모를 이용한 태풍강우량 추출기법은 과거 태풍 감시구역을 이용한 강우량 추출기법의 한계를 보완할 수 있을 것으로 판단되며, 유역별, 행정구역별로 태풍이 야기한 직접적인 강우를 추출할 수 있으며 주관적인 판단에 의해 발생할 수 있는 태풍강우의 과대 및 과소평가의 위험성을 최소화 할 수 있다. 본 연구의 결과는 향후 태풍과 집중호우의 특성분석과 기상인자를 활용한 강우예측, 수공구조물 설계, 산사태, 토석류에 대한 사전 대비책 등에 활용될 수 있을 것으로 기대된다.

Yield and Chemical Composition of Cassava Foliage and Tuber Yield as Influenced by Harvesting Height and Cutting Interval

  • Khang, Duong Nguyen;Wiktorsson, Hans;Preston, Thomas R.
    • Asian-Australasian Journal of Animal Sciences
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    • 제18권7호
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    • pp.1029-1035
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    • 2005
  • A 3${\times}$4 factorial field experiment with a complete randomised split-plot design with four replicates was conducted from June 2002 to March 2003 at the experimental farm of the Nong Lam University, Ho Chi Minh City, Vietnam, to determine effects of different harvesting heights (10, 30 and 50 cm above the ground) and cutting intervals (45, 60, 90 and 285 days) on yield of foliage and tubers, and chemical composition of the foliage. Cassava of the variety KM 94 grown in plots of 5 m${\times}$10 m at a planting distance of 30 cm${\times}$50 cm was hand-harvested according to respective treatments, starting 105 days after planting. Foliage from the control treatment (285 days) and all tubers were only harvested at the final harvest 285 days after planting. Dry matter and crude protein foliage yields increased in all treatments compared to the control. Mean foliage dry matter (DM) and crude protein (CP) yields were 4.57, 3.53, 2.49, and 0.64 tonnes DM $ha^{-1}$ and 939, 684, 495 and 123 kg CP $ha^{-1}$ with 45, 60, 90 and 285 day cutting intervals, respectively. At harvesting heights of 10, 30 and 50 cm the DM yields were 4.27, 3.67 and 2.65 tonnes $ha^{-1}$ and the CP yields were 810, 745 and 564 kg $ha^{-1}$, respectively. The leaf DM proportion was high, ranging from 47 to 65%. The proportion of leaf and petiole increased and the stem decreased with increasing harvesting heights and decreasing cutting intervals. Crude protein content in cassava foliage ranged from 17.7 to 22.6% and was affected by harvesting height and cutting interval. The ADF and NDF contents of foliage varied between 22.6 and 30.2%, and 34.2 and 41.2% of DM, respectively. The fresh tuber yield in the control treatment was 34.5 tonnes $ha^{-1}$. Cutting interval and harvesting height had significant negative effects on tuber yield. The most extreme effect was for the frequent foliage harvesting at 10 cm harvesting height, which reduced the tuber yield by 72%, while the 90 day cutting intervals and 50 cm harvesting height only reduced the yield by 7%. The mean fresh tuber yield decreased by 56, 45 and 27% in total when the foliage was harvested at 45, 60 and 90 day cutting intervals, respectively. It is concluded that the clear effects on quantity and quality of foliage and the effect on tuber yield allow alternative foliage harvesting principles depending on the need of fodder for animals, value of tubers and harvesting cost. An initial foliage harvest 105 days after planting and later harvests with 90 days intervals at 50 cm harvesting height increased the foliage DM and CP yield threefold, but showed only marginal negative effect on tuber yield.

The Relationship between Temperature Patterns and Urban Morfometri in the Jakarta City, Indonesia

  • Maru, Rosmini;Ahmad, Shaharuddin
    • Asian Journal of Atmospheric Environment
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    • 제9권2호
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    • pp.128-136
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    • 2015
  • Sky View Factor (SVF) is one of the urban morfometri parameters that impact on the Urban Heat Island (UHI). SVF analisys was conducted in the city of Jakarta to investigate the relationship between urban temperature with urban morfometri. Jakarta City is the most populous city in the world that has a surrounding area $66,152km^2$ and the total population around 23 million people. The population of the city is the sixth highest in the world today. SVF measurements done by taking pictures at the six stations that have different morphological characteristics namely (1) the narrow streets Apartment Cempaka Mas (JS ITC), (2) the width of the road Apartment Cempaka Mas (JL ITC), (3) in front of Colleges Kanisius (DKK), (4) in front of office Journalist of Indonesia (DKWI), (5) Utan Kayu (UK), and (6) Tambun (TB). SVF value is obtained from the photgraphic image. Taking pictures at the location using a Nikon D90 camera with a Nikon Fisheye Nikkor 10.5 mm 1 : 2.8 G ED, further processed through a global mapper program. Therefore, the SVF derived from the six stations that vary 0.21 to 0.78. Temperature measurement is done during daylight hours from 06:00 am to 18:00 pm during the Western Part of Indonesia (WIB). Measurements performed at three different times, namely working days (HK) regular holidays (HCB) national holidays (HCN). The results showed that the highest average temperature of $33.32^{\circ}C$, occurring at UK station (SVF=0.45) at the time of HCB. Meanwhile, the average low temperature of $31.22^{\circ}C$ occurred at JLITC station (SVF=0.42). The two-time occurred on ordinary holidays. Maximum temperature of $38.4^{\circ}C$ occurred in Utan Kayu station (SFV=0.45) that occurred at 11.00 hrs, normal holidays. Furthermore minimum temperature 24.5 occurred at Tambun station (SVF=0.78) at 06.00 hrs in the morning at the usual holidays and national holidays. In general, the results showed that areas with large SVF has a lower temperature compared with areas with smaller SVF. Though, are not the only factors that matter, but this research may show that an increase in temperature in the city of Jakarta. Therefore, it is necessary to mitigate the serious from the government or society.

한국과 일본의 초등학교 수학교과서 비교 연구 - 4학년을 중심으로 - (A Comparative Study of Elementary School Mathematics Textbooks between Korea and Japan - Focused on the 4th Grade -)

  • 이재춘;김선유;강홍재
    • 한국초등수학교육학회지
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    • 제13권1호
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    • pp.1-15
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    • 2009
  • 2009년부터 1, 2학년 수학교과서를 시작으로 학년별 새 교과서 편찬이 연차적으로 이루어지고 있다. 2009년 현재 시범학교에서 3, 4학년 실험본 수학교과서로 수업을 하고 있으며, 이 1년간의 실험 수업을 통해서 장 단점을 파악해 내년 3, 4학년의 새 수학교과서가 나을 예정이다. 본 연구는 한국과 일본 양국의 초등학교 4학년 수학교과서를 비교 분석하여 그 시사점을 통해 개정 교육과정의 수학교과서 편찬에 유용한 참고 자료를 제공하는데 그 목적을 두고 있다. 이를 위하여 비교 분석의 대상을 교육인적자원부에서 발행한 4-가, 4-나 단계의 수학교과서 2권과 일본의 계림관(啓林館)에서 발행한 4-상(上), 4-하(下)의 산수교과서 2권으로 하여 교과서 단원명과 단원체제 및 영역별 지도내용을 비교하여 그 시사점을 도출하였다.

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DENSE MOLECULAR CLOUDS IN THE GALACTIC CENTER REGION II. H13CN (J=1-0) DATA AND PHYSICAL PROPERTIES OF THE CLOUDS

  • Lee, Chang-Won;Lee, Hyung-Mok
    • 천문학회지
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    • 제36권4호
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    • pp.271-282
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    • 2003
  • We present results of a $H^{13}CN$ J=1-0 mapping survey of molecular clouds toward the Galactic Center (GC) region of $-1.6^{\circ}{\le}{\iota}{\le}2^{\circ}$ and $-0.23^{\circ}{\le}b{\le}0.30^{\circ}$ with 2' grid resolution. The $H^{13}CN$ emissions show similar distribution and velocity structures to those of the $H^{12}CN$ emissions, but are found to better trace the feature saturated with $H^{12}CN$ (1-0). The bright components among multi-components of $H^{12}CN$ line profiles usually appear in the $H^{13}CN$ line while most of the dynamically forbidden, weak $H^{12}CN$ components are seldom detected in the $H^{13}CN$ line. We also present results of other complementary observations in $^{12}CO$ (J=1-0) and $^{13}CO$ (J=1-0) lines to estimate physical quantities of the GC clouds, such as fractional abundance of HCN isotopes and mass of the GC cloud complexes. We confirm that the GC has very rich chemistry. The overall fractional abundance of $H^{12}CN$ and $H^{13}CN$ relative to $H_2$ in the GC region is found to be significantly higher than those of any other regions, such as star forming region and dark cloud. Especially cloud complexes nearer to the GC tend to have various higher abundance of HCN. Total mass of the HCN molecular clouds within $[{\iota}]{\le}6^{\circ}$ is estimated to be ${\~}2 {\times}10^7\;M_{\bigodot}$ using the abundances of HCN isotopes, which is fairly consistent with previous other estimates. Masses of four main complexes in the GC range from a few $10^5$ to ${\~}10^7\;M_{\bigodot}$ All the HCN spectra with multi-components for the four main cloud complexes were investigated to compare the line widths of the complexes. The largest mode (45 km $s^{-1}$) of the FWHM distributions among the complexes is in the Clump 2. The value of the mode tends to be smaller at the farther complexes from the GC.

ON THE GALACTIC SPIRAL PATTERNS: STELLAR AND GASEOUS

  • MARTOS MARCO;YANEZ MIGUEL;HERNANDEZ XAVIER;MORENO EDMUNDO;PICHARDO BARBARA
    • 천문학회지
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    • 제37권4호
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    • pp.199-203
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    • 2004
  • The gas response to a proposed spiral stellar pattern for our Galaxy is presented here as calculated via 2D hydrodynamic calculations utilizing the ZEUS code in the disk plane. The locus is that found by Drimmel (2000) from emission profiles in the K band and at 240 ${\mu}m$. The self-consistency of the stellar spiral pattern was studied in previous work (see Martos et al. 2004). It is a sensitive function of the pattern rotation speed, $\Omega$p, among other parameters which include the mass in the spiral and its pitch angle. Here we further discuss the complex gaseous response found there for plausible values of $\Omega$p in our Galaxy, and argue that its value must be close to $20 km s^{-l}\;kpc^{-1}$ from the strong self-consistency criterion and other recent, independent studies which depend on such parameter. However, other values of $\Omega$p that have been used in the literature are explored to study the gas response to the stellar (K band) 2-armed pattern. For our best fit values, the gaseous response to the 2-armed pattern displayed in the K band is a four-armed pattern with complex features in the interarm regions. This response resembles the optical arms observed in the Milky Way and other galaxies with the smooth underlying two-armed pattern of the old stellar disk populations in our interpretation. The complex gaseous response appears to be related to resonances in stellar orbits. Among them, the 4:1 resonance is paramount for the axisymmetric Galactic model employed, and the set of parameters explored. In the regime seemingly proper to our Galaxy, the spiral forcing appears to be marginally strong in the sense that the 4:1 resonance terminates the stellar pattern, despite its relatively low amplitude. In current work underway, the response for low values of $\Omega$p tends to remove most of the rich structure found for the optimal self-consistent model and the gaseous pattern is ring-like. For higher values than the optimal, more features and a multi-arm structure appears.

항공기를 이용한 온실가스 CO2와 CH4의 연속관측: 안면도 겨울철 연직분포사례 분석 (Airborne In-situ Measurement of CO2 and CH4 in Korea: Case Study of Vertical Distribution Measured at Anmyeon-do in Winter)

  • 이선란;구태영;문혜진;;;오영석;이해영;변영화
    • 대기
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    • 제29권5호
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    • pp.511-523
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    • 2019
  • A new Korean Meteorological Administration (KMA) airborne measurement platform has been established for regular observations for scientific purpose over South Korea since late 2017. CRDS G-2401m analyzer mounted on the King Air 350HW was used to continuous measurement of CO2, CH4 and CO mole fraction. The total uncertainty of measurements was estimated to be 0.07 ppm for CO2, 0.5 ppb for CH4, and 4.2 ppb for CO by combination of instrument precision, repeatability test simulated in-flight condition and water vapor correction uncertainty. The airborne vertical profile measurements were performed at a regional Global Atmosphere Watch (GAW) Anmyeon-do (AMY) station that belongs to the Total Carbon Column Observing Network (TCCON) and provides concurrent observations to the Greenhouse Gases Observing Satellite (GOSAT) overpasses. The vertical profile of CO2 shows clear altitude gradient, while the CH4 shows non-homogenous pattern in the free troposphere over Anmyeon-do. Vertically averaged CO2 at the altitude between 1.5 and 8.0km are lower than AMY surface background value about 7 ppm but higher than that observed in free troposphere of western pacific region about 4 ppm, respectively. CH4 shows lower level than those from ground GAW stations, comparable with flask airborne data that was taken in the western pacific region. Furthermore, this study shows that the combination of CH4 distribution in free troposphere and trajectory analysis, taking account of convective mixing, is a useful tool in investigating CH4 transport processes from tropical region to Korean region in winter season.