• YAKHAK HOEJI
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• v.37 no.2
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• pp.129-135
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• 1993

Serratia sp. Y-4 was isolated from soil. Many characteristics of the strain and optimum cultivation condition for protease production were investigated.,The protease from Serratia sp. Y-4 was purified and studied for the properties of the enzyme. The isolated strain was identified to the genus Serratia. The strain was cultivated in 1%-casein, 0.5%-Na$_{3}$PO$_{4}$.7H$_{2}$O, 0.1%-NaCl, 0.05%-KCI, 0.02%-MgSO$_{4}$.7H$_{2}$O, 0.02%-CaCl$_{2}$.2H$_{2}$O, 0.02%-ZnSO$_{4}$.7H$_{2}$O, 0.02%-MnCl$_{2}$.4H$_{2}$O and 0.5%-soy bean oil at pH 7.0 for 35 hrs. The enzyme was purified about 5.89 fold from the culture broth with 31.1% recovery and 19,613 u/mg through ultrafiltration, ammonium sulfate, DEAE-sephacel and Superose-12 chromatography. The purified enzyme was identified as one band by isoelectric focusing, SDS- and native-PAGE. It has maxium activity at $37^{\circ}C$ and pH 9.0. Molecular weight of it is approx. 50 kD and pl is about 6.70. Its Km value for casein was 20 mg/ml. 5 mM-EDTA, 5mM-SDS, Ag$^{+1}$, Cu$^{+2}$, Hg$^{+2}$ and Pb$^{+2}$ inhibited the enzyme.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.20 no.5
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• pp.9-13
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• 2020

Sasang Constitutional Medicine is a traditional Korean medicine optimized for personalized medicine, and despite its effective clinical efficacy, the inaccuracy of constitutional diagnosis has been pointed out as a limitation. To improve the accuracy, a constitutional analysis algorithm based on quantitative data was developed. In this study, a constitutional analysis experience system applied with the algorithm was developed and repeatability was evaluated. The system analyzes the constitution of the experiencer by collecting front and side facial images, audio, and questionnaire and calculating the integrated constitution probability value. To evaluate the repeatability of the probability values of the system was performed five times each for three people, and the coefficient of variation was 4.778%, indicating that the repeatability was sufficient. The system could contribute to the promotion of the awareness of Sasang medicine.

• Transactions of the Korean Society of Automotive Engineers
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• v.13 no.6
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• pp.135-141
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• 2005

Currently many safety assessment tests are conducted by crashing a vehicle against a rigid or deformable barrier. It is quite rational to evaluate crash performance of a vehicle in a barrier test in terms of vehicle stiffness and strength. However, there has been a lot of debate on whether barrier testing is a duplicate of real world crash collisions. One of the issues is car to car compatability. There are two essential subjects in compatability. One is partner-protection when crashing into another vehicle and the other is self-protection when struck by another vehicle. When considering a car to car frontal crash between a mini car and a large heavy car, it is necessary to evaluate human body stiffness of each vehicle. In this study, in order to evaluate the compatability of cars in car-to-car crashes, four tests were conducted. Test speed of each car is 48.3km/h, and the overlap of the mini and large car is $40\%$, and the overlap of the small cars is $100\%$. In all tests, only a drive dummy is used. The test results of the car to car crash test show that vehicle safety standard of mini car is not satisfied compared with large heavy car and HIC value of mini car is higher than large car. In this case observed that the relatively lower stiffness and weight of the mini car resulted in absorbing a large share of the total input energy of the system when crashed into the large heavy car.

• Korean Journal of Microbiology
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• v.16 no.3
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• pp.122-130
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• 1978

During the sporulation stage in Saccharomyces cerevisiae J170, the incorporation of $D^{14}$ C-glucose into starved cells of sporulation stage as well as the vegetative one is appeared higher at pH 6.0. Glucose transport system, in both the vegetative and sporulation stage, is associated with "energy dependent" as the result of repression by such a respiratory inhibitor as 2, 4-dinitrophenol. The Km value of glucose uptake in vegetative stage and sporulation stage was 2.1 mM and 2.5 mM respectively, indicating that the glucose is considerably reuqired for vegetative growth. Competition and countertranspoer of glucose by frutose and galactose are more distinct in vegetative stage, comparing with sporulation stage. The main sugar components of yeast cells consists of ribose, mannose, and ${\alpha}, \;{\beta}-glucose$. Amounts of mannose is lower in the aporulation stage than that in the vegetative stage.

• Proceedings of the Korean Geotechical Society Conference
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• 2002.03a
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• pp.751-758
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• 2002

Boreholes that are drilled in soft or unconsolidated materials such as gravels and coals are prone to collapse. To maintain the hole, some kinds of casing pipes are needed. If thereby a plastic pipe e.g. PVC is used for the casing, Televiewer tool is still capable of detecting structural features such as fractures in the borehole wall behind the pipe, whereas other borehole-imaging logging devices such as BIPS (Borehole Image Processing System) and FMS(Formation Micro Scanner) won't provide any information about that. Televiewer's primary component is a piezoelectric transducer centered in the hole. It acts as both a transmitter and receiver, and sends an ultrasonic beam. That is reflected, in the same manner as the seismic wave propagation, from the both sides(inner and outer surfaces) of the casing pipe, transmits through the pipe and then reflected from the borehole wall. With an appropriate choice of time-windowing, it is possible to capture the returning signals from both the borehole wall and the outer side of casing pipe as well. A suite of laboratory tests were performed on various physical models composed of plastic pipes with different diameters. Although the amplitudes of returning signals were reduced to about half the usual value due to the transmission loss, the dynamic range of Televiewer tool was sufficient to observe the structural features behind the casing pipe. Besides, several representative case studies at various research areas in our country are presented. The results demonstrate the usefulness of the transmissivity of Televiewer acoustic km, which will assist in further structural interpretation.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1979.04a
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• pp.113.2-114
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• 1979

$\beta-Galactosidase$ is an enzyme which catalizes hydrolysis of lactose, a natural substrate, to glucose and galctose and transferring some monosac-charide units to active acceptors as sugar or alcohol. The occurence of $\beta-Galactosidase$ is known in various microorganisms, animals and higher plants and has been studied by many investigatigators. Especially, a great deal of articles for the enzyme of E. coli have been presented in genetic control mechanism and induction-repression effects of proteins, On the other hand, in the dairly products industry, it is important to hydrolyes lactosd which is the principal sugar of milk and milk products. During the last few years, the interest in enzymatic hydrolysis of milk lactose has teen increased, because of the lactose intolerence in large groups of the population. Microbial $\beta-Galactosidases$ are considered potentially most suitable for processing milk to hydrolyse lactose and, in recent years, the immobilized enzyme from yeast has been examined. Howev, most of the microbial $\beta-Gal$ actosidase are intracellular enzymes, except a few fungal $\beta-Gala-$ ctosidases, and extracellular $\beta-Galactosidase$ which may be favorable to industrial applieation is not so well investigated. On this studies, a mold producing a potent extracellular $\beta-Galactosidase$ was isolated from soil and identified as an imperfect fungus, Beauveria bassians. In this strain, both extracellular and intracellular $\beta-Galactosidases$ were produced simultaneously and a great increase of the extracellular production was acheved by improving the cultural conditions. The extracellular enzyme was purified more than 1, 000 times by procedures including Phosphocellulose and Sephadex G-200 chromatographies. Several characteristics of the enzymewas clarified with this preparation. The enzyme has a main subunit of molecular weight of 80, 000 which makes an active aggregate. And at neutral pH range, it has optimum pH for activity and stability. The Km value was determined to be 0.45$\times$10$^{-3}$ M for $o-Nitrophenyl-\beta-Galactoside.$ In any event, it is interesting to sttudy the $\beta-Galactosidase$ of B. bassiana for the mechanism of secretion and conformational structure of enzyme.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.23 no.4
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• pp.47-58
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• 2020

This study was done to evaluate resources distributed around the Nakdong-Jeongmaek which is the eastern ridge line of the Nakdong-river basin with 437km in length. Here we found and/or searched for thousands of resources within each of 210 villages around the ridge, which were divided into six categories including landscape, natural park, protected area, history, tourism and biodiversity. An inventory was also created using an attribute table in a shape file for identifying the spatial location and property for every resource existing in each village. Each of fields for six-typed resources has 210 records representing each village and resources within it. If a resource exists in a village, '1' is assigned for its existence in its corresponding record. Otherwise, '0' is assigned for its non-existence in the record. The number of '1' on six records for a village is meaning the number of resources contained within a village, which can be a barometer to decide the properties of each village. In this study, we found five core villages containing all kind of resources in it, while 52 villages were found having only a single type of resources within it. The other villages were known to have multiple resources like having two or more ones.

• Korean Journal of Microbiology
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• v.9 no.1
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• pp.39-45
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• 1971

During the sporulation stage in Saccharomyces cerevisiae J170, the incorporation of D$^{14}$ C-glucose into starved cells of sporulation stage as well as the vegetative one is appeared higher at pH 6.0. Glucose transport system, in both the vegetative and sporulation stage, is associated with "energy dependent" as the result of repression by such a respiratory inhibitor as 2,4-dinitrophenol. The Km value of glucose uptake in vegetative stage and sporulation stage was 2.1 mM and 2.5 mM respectively, indicating that the glucose is considerably reuqired for vegetative growth. Competition and countertranspoer of glucose by frutose and galactose are more distinct in vegetative stage, comparing with sporulation stage. The main sugar components of yeast cells consists of ribose, mannose, and .apha., .betha.-glucose. Amounts of mannose is lower in the aporulation stage than that in the vegetative stage.ive stage.

• Korean Journal of Microbiology
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• v.18 no.4
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• pp.161-171
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• 1980

A mutant strain having increased productivity of both enzymes, protease and amylase, was obtained from A. flavus KU 153, isolatd from South Korea for its high protease production by successive ultra-violet light irradiation, Two glucoamylases from the mutant strain selected were purified from wheat branculture by successive salting out, followed by dialysis and column chromatography, and their characteristics were compared with those of the wild strain. Glucoamylase production of the mutant selected was increased about 3.3 times compared with the wild strain, and 2.1 times compared with the parental strain, ${\alpha}-amylase$ activity of the mutant selected was about 2 times hugher than that of the wild strain or the parental strain. Protease and cellulase productivities of the muant selected were all alike compared with those of the highly proteolytic mutant, the parental strain. Therefore, it was considered that the back mutation on the protease production did not occurred in the formation process of the glucoamylase producing mutant. Total activities of glucoamylase I and II from the mutant selected were 2.86 and 3.65 times higher compared with those from the wild strain, respectively. Considering the optimal pH-thermal stability and Km-Vmax value of glucoamylase I and II from both strains, wild and mutant, it was deduced that the characteristics of glucoamylase I and II from the wild strain did not altered during the mutation process. Therefore, it was concluded that the selected mutant did not induce the formation of another glucoamylase isozyme, or the changes in the characteristics of the glucoamylase, but induce the productivity of the same glucoamylase I and II by the action of regulatory gene.

• Journal of Marine Bioscience and Biotechnology
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• v.3 no.1
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• pp.1-6
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• 2008

Angola has a coastline of about 1,650 km long. Two diverging current namely, the Angola current with its warm water from the north and the cold Benguella Current in the south create a strong up-welling with a high productive ecosystem for marine resources. The area from Lobito to the mouth of the Cunene River, also known as the Southern fishing zone is by far the most productive of Angola's fishing zones. In 1977, the total potential of its marine fisheries sector was estimated at more than 700,000 tonnes per annum. In 2003, the Total Allowable Catch (TAC) established for demersal species was 57,600 tonnes and 160,000 tonnes for pelagic species. The most important resources are various marine demersal and pelagic fish including pilchard and the Cape and Cunene horse Mackerel (Tranchurus capensis and T. trecae). Sardinellas (Sardinella aurita and S. maderensis) are fished in parallel with horse mackerel. The rest of the catches are mainly demersal spp. and some deep water crustaceans. The demersal sppecies consist of Hake (Merluccius polli and M. capensis) and the large eye dentex spp. Tunas are caught at certain times of the year whilst some marine shrimp are also harvested from the Angolan waters. Angola also has several high value freshwater fish species, exploited by about 255 fishers. Tilapia sp. is among the most important and abundant fresh water fish found in Angola. Other species include the catfish (Clarias gariepinus) and fresh water prawns (Macrobrachuin rosenbergii). Some aquaculture ponds have been established in the country side, but due to lack of investment, proper training and the impact of civil war have seriously limited developments in the sub sector.