• Structural Engineering and Mechanics
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• 제61권4호
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• pp.527-538
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• 2017

The evaluation of the loss-of-support conditions of frictional beam-to-column connections using simplified numerical models describing the transverse response of a portal-like structure is presented in this paper considering the effects of the seismic-hazard disaggregation. Real earthquake time histories selected from European Strong-motion Database (ESD) are used to show the effects of the seismic-hazard disaggregation on the beam loss-of-support conditions. Seismic events are classified according to different values of magnitudes, epicentral distances and soil conditions (stiff or soft soil) highlighting the importance of considering the characteristics of the seismic input in the assessment of the loss-of-support conditions of frictional beam-to-column connections. A rigid and an elastic model of a frame of a precast industrial building (2-DoF portal-like model) are presented and adopted to find the minimum required friction coefficient to avoid sliding. Then, the mean value of the minimum required friction coefficient with an epicentral distance bin of 10 km is calculated and fitted with a linear function depending on the logarithm of the epicentral distance. A complete parametric analysis varying the horizontal and vertical period of vibration of the structure is performed. Results show that the loss-of-support condition is strongly influenced by magnitude, epicentral distance and soil conditions determining the frequency content of the earthquake time histories and the correlation between the maxima of the horizontal and vertical components. Moreover, as expected, dynamic characteristics of the structure have also a strong influence. Finally, the effect of the column nonlinear behavior (i.e. formation of plastic hinges at the base) is analyzed showing that the connection and the column are a series system where the maximum force is limited by the element having the minimum strength. Two different longitudinal reinforcement ratios are analyzed demonstrating that the column strength variation changes the system response.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2013년도 춘계학술대회
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• pp.500-502
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• 2013

광역상수관, 가스관 등과 같은 금속성의 지중 매설물은 전력유도 현상에 대한 차폐 효과를 갖게 된다. 일반적으로 인구와 건물이 밀집되어 있는 도시 지역에서는 금속성의 지중 매설물이 많이 분포하게 되어 동일한 유도원에 의한 전력 유도 현상에 대하여 더 높은 차폐 효과를 기대할 수가 있으며 이를 도시 차폐 계수라 한다. 이러한 도시 차폐 계수의 효과를 실증하기 위하여 고속 전철이 경과하는 도시 환경과 시골 환경으로 분류 가능한 개소를 각각 30개소씩 선정하여 고속 전철 선로와 병행하는 1km의 통신선을 임의 포설하고 전력 유도 종전압을 측정하였으며 이 측정 결과를 분석하여 도시 차폐 계수치를 도출하였다. 하지만 이렇게 도출된 도시 차폐 계수를 일반적인 도시와 시골 환경을 갖는 지역에 일괄적으로 적용하기에는 정확한 도시와 시골의 구분 기준의 모호함 등의 문제에 직면하게 된다. 이에 본 논문에서는 도시와 시골의 구분 방법론의 하나로서 해당 지역의 적용 범위 내 분포하는 건물의 밀집도를 이용하는 방안을 소개하고자 한다.

• 한국식품영양과학회지
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• 제19권5호
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• pp.434-442
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• 1990

Aspergillus sp. CC-29 ws selected for its strong protease activity among various stains of molds found in soil. It was found that the production of alkaline protease reached to maximum when the wheat bran medium containing glucose as carbon source had been cultured for 4 days. Alkaline proteased was purified 36.10 fold from Aspergillus sp. CC-29 The purification procedures included ammonium sulfate fractunation gel filteration on Sepha-dex G-75 G-150 and DEAE-cellulose ion-exchange chromatography, The yield of the purified enzyme was 22.40% The purified enzyme was confirmed as a single band by the polyacryla-mide. When the purified enzyme was applied to SDS-PAGE the molecular weight was estima-ted 24000. The optimum pH for the enzyme activity was 9.0 and the optimum temperature was 4$0^{\circ}C$ The reaction of this enzyme followed typical Michaelis-Menten kinetics with the Km value of 2.10$\times$10-4M with the Vmax of 29.41 $\mu$g/min. The enzyme was reactively stable in alkalic condition and unstable by heat treatment. The activity of alkaline protease was increased by the addition of Ca2+ whereas it was inhibited by Hg2+ Zn2+ at concentration of 1$\times$10-3M.

• 한국대기환경학회지
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• 제30권2호
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• pp.119-127
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• 2014

A very high resolution weather analysis system (VHRAS) of 50 m horizontal resolution is established based on LAPS. VHRAS utilizes the 3 hourly forecast data of the Unified Model (UM) of the Korea Meteorological Administration (KMA) with the horizontal resolution of 12 km as initial guess fields. The analysis system ingests the automatic weather station (AWS) data as input observations. The analysis system operates every hour for Seoul, Korea region in real time basis. It takes less than 10 minutes for one analysis cycle. The size of grid of the analysis domain is $800{\times}660$, respectively. The analysis results from December 2010 to February 2011 showed that the mean biases of temperature, maximum and minimum temperature were -0.07, 1.6, $0.2^{\circ}C$, respectively. The temperature in the central part of the city revealed relatively higher value than that of the surrounding mountainous areas, which showed a heat island feature. The heat island appears in zonal direction since the central city region is developed along a large river. Along the heat island, the eastern region was warmer than the western region. The warmer temperature in the western part of the heat island was caused by anthropogenic heat change in conjunction with the change of land use. This system will provide more reliable weather data and information in Seoul.

• Journal of Microbiology
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• 제39권4호
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• pp.279-285
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• 2001

Vibrio parahaemolyticus is a halophilic bacterium associated with seafood gastroenteritis. An unusual strain of Kanagawa-positive urease producing Vibrio parahaemolyticus O1:K1 was isolated from the environment and identified . A polymerase chain reaction assay revealed that this strain harbored both the tdh and the genes. The urease from this strain was studied. Maximum urease production was induced in LB medium containing 0.2% urea, 0.5% glucose, 2% NaCl and pH 5.5 with 6h of culti-vation at 37$\^{C}$ under aeration. Purification of urease was achieved by the process of whole cell lysate, 65% ammonium sulphate precipitation, DEAE-cellulose ion exchange column chromatography, Sepharose CL-6B gel filtration and oxirane activated Sepharose 6B-urea affinity chromatography with 203 fold purification and 2.2% yield. Analysis of the purified enzyme by SDS-PAGE demonstrated the presence of the subunits with a molecular weight of 85kDa, 59kDa, 41kDa and the molecular weight for the native enzyme by nondenaturing PAGE and gel filtration chromatography was 255kDa. The purified urease was stable at pH 7.5 and the opeimal pH in HEPES buffer was 8.0 The enzyme was stable at 60$\^{C}$ for 2 h with a residual activity of 32% . The addition of 10$\mu$M if NiCl$_2$maintained stability for 30 min. The Km value of the purified enzyme was 35.6 mM in urea substrate. The TD$\_$50/(median toxic dose) of the purified urease was 2.5$\mu\textrm{g}$/ml on human leukemia cells.

• 미생물학회지
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• 제14권2호
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• pp.65-74
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• 1976

Atternaria sp. was isolated from soil and crude cellulases were prepared from wheat bran culture of the fungus. The activities of the crude enzyme were studied on five different subvstrates and some phsical properties were also examined, crude enzymes were purified by column chromatography on DEAE Sephadex and Sephadex, Isozymes were separated some of which were active specifically on DEAE cellulose and some were primarily active on cellulose and CM-cellulose. The optimal points of pH and temperature for the crude enzyme were varied depending on the substrates ; On cellulose they were at pH 6.0 and 40.deg.C, on CM-cellulose at pH's 4.0 and 6.0 and 60.deg.C, and on DEAW-cellulose at pH 5.0 and 50.deg.C. Two active fractions, F-1 and F-II on Na-CMC was used as substrate the Km values of crude enzyme, F-I and F-II were calculated to be $4{\times}10^{-5}$ , 1.1 * 10$^{-4}$ , and $1.25{\times}10^{-4}mN$ resepctively. The Ki value of $Cu^{++}$ for crude enzyme was$4{\times}^{-4}mN$ , while that of $Nm^{++}$ while in the same concentration of $Mn^{++}$ it reached to 91%. Some 57% activity of F-1 was inhibited in s mN $Cu^{++}$, whereas it was inhibited as much as 81% in the same concentration above the concentration of 0.3 mM with tis activity reaching up to 137% in 2 mM. On the other hand the F-11 was inhibited by the presence of M $n^{++}$ and some 67% activity was inhibited at 2mM.

• 한국환경복원기술학회지
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• 제18권4호
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• pp.15-27
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• 2015

The objectives of this study are to analyze the quantitative ecological principal components of Korean Peninsula using the multivariate spatial environmental datasets and to compare the ecological difference between South and North Korea. Ecological maps with GIS(Geographical Information System) are constructed by PCA(Principal Component Analysis) based on seventeen raster(cell based) variables at 1km resolution. Ecological differences between South and North Korea are extracted by Factor Analysis using ecosystem maps masked from Korean ones. Spatial data include SRTM(Shuttle Radar Topography Mission), Temperature, Precipitation, SWC(Soil Water Content), fPAR(Fraction of Photosynthetically Active Radiation) representing for a productivity, and SR(Solar Radiation), which all cover Korean peninsula. When it performed PCA, the first three scores were assigned to red, green, and blue color. This color triplet indicates the relative mixture of the seventeen environmental conditions inside each ecological region. The first red one represents for 'physiographic conditions' worked by high elevation and solar radiation and low temperature. The second green one stands for 'seasonality' caused by seasonal variations of temperature, precipitation, and productivity. The third blue one means 'wetness condition' worked by high value such as precipitation and soil water contents. FA extraction shows that South Korea has relatively warm and humid ecosystem affected by high temperature, precipitation, and soil water contents whereas North Korea has relatively cold and dry ecosystem due to the high elevation, low temperature and precipitation. Results would be useful at environmental planning on inaccessible land of North Korea.

• 자원환경지질
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• 제31권5호
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• pp.399-413
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• 1998

Daegang granite is located around the Namwon-gun, Cheolabuk-do, and is an elongate stock $(80 km^{2})$ in the NNE-SSW direction. Daegang granite has the very same mineralogical and geochemical characteristics as those of the typical A-type granites; (1) it is a one feldspar hypersolvus granite, and is classified as an alkali feldspar granite in the lUGS scheme, (2) has small amounts of Fe-rich biotite (annite) and alkali amphibole (ribeckite) that are late in the crystallization sequence of the granitic magma, (3) always contains opaque oxides, fluorite and zircon, (4) shows high and quite homogeneous $SiO_2$, content (mostly 72~77 wt.%) and $(Na_{2}O+K_{2}O)/Al_{2}O_{3}$ ratio (0.90~0.98), (5) contains high Ga, lOOOO*Ga/Ai, $K_{2}O+Na_{2}O$, $(K_{2}O+Na_{2}O)/CaO$, $K_{2}O/MgO$, FeO/MgO, agpaitic index, Zr, Nb, Ce, Y, Zn value or ratio that resemble to those of the Australian A-type granites (Whalen et al., 1987), and (6) has enriched LREE and HREE that show flat variation pattern with slightly depleted in HREE and profound Eu anomalies (Eu/Eu*=0.04~0.l4). In the tectonic discrimination diagrams of Pearce et al. (1984) and Eby (1992), Daegang granite is classified as a within plate granite and $A_{2}-type$.

• 자원환경지질
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• 제29권3호
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• pp.335-343
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• 1996

The dipole-dipol electrical resistivity survey was conducted to investigate the probable contamination of the Han river by leachate from the near-by Nanjido Landfill. The survey line of 3 km was set along the unpaved road toward the Han river. For the convenience of the field work, the survey line was divided into four segments. The complete two-dimensional resistivity section was constructed by connecting the inversion result of each segment. Gravity survey was also carried out along the profile parallel to the resistivity line. Near surface resistivity generally appeared to be of very low value in most part of the survey area and the boundary between the alluvium layer and underlying basement rocks is well discriminated on the resistivity section. These results agree well with those of the preceding Schlumberger depth sounding made at adjacent area by Lee and fun (1995). The variation of thickness of the alluvium layer delineated by gravity anomaly profile also correlates well with the result of the resistivity survey on the qualitative basis. The problem of contamination by leachate from the Nanjido Landfill, where various waste materials have been dumped without any proper treatment facilities, has been remains unsolved yet. Therefore, we present the most probable passages of leachate flow based on the survey results and have briefly discussed about measure for contamination control. Considering the thickness of alluvium and the possible existence of fractured zone, the middle point between 1st and 2nd landfill and the midst of 1st landfill are the most hazardous regions to make leachates flow into the Han river. Since large amounts of leachates are observed from the test wells located on the lines extending from the border between the 1st and 2nd landfill and the middle of the lst landfill, contamination protection barriers are strongly recommended near these regions.

• Fisheries and Aquatic Sciences
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• 제21권4호
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• pp.9.1-9.7
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• 2018

To establish a comprehensive management strategy, as part of the optimization of cultural practice for an oyster rack culture system, we used a numerical model to estimate the primary production in the waters on the eastern coast of Wando island, South Korea. The estimated primary production ranged from 17.12 to $1052.55mgC\;m^{-2}day^{-1}$ ($204.22{\pm}224.75mgC\;m^{-2}day^{-1}$ in average). Except for the times of peak phytoplankton blooms, the estimated primary production (PP) was consistently under $200mgC\;m^{-2}day^{-1}$, which is more similar to the value of PP measured off the western coast of South Korea than the southern coast. No clear relationship was observed between nitrogen content and rainfall with the exception of heavy rainfall events, indicating that precipitation might not be the main source of nutrients in these waters. No clear influence was observed from Doam tidal discharge, located 24 km north from these waters due to main tide comes in this area from the channel between Gunwe-myeon in Wando island and Pukpyeong-myeon in Haenam-gun. Because of the shallow water depth and strong tidal current, resuspension of sediments, which causes an input of nitrogen into the system, could be easily caused by even mild wind and the infrequent passing of ships. Microscopic examination of the phytoplankton composition showed additional contribution of benthic species such as Paralia sulcata into the waters, which increase the productivity of oyster farms in the waters. The availability of nitrate and phosphate for primary production was temporarily limited throughout most of the spring and autumn blooming season.