• 제목/요약/키워드: Km value

검색결과 892건 처리시간 0.026초

Pseudomonas sp. YD-15가 생산하는 CMCase의 특성 (Properties of the CMCase produced by Pseudomonas sp. YD-15)

  • 이정우;김창남;허남윤;오두환
    • Applied Biological Chemistry
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    • 제35권3호
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    • pp.173-178
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    • 1992
  • 토양으로부터 분리된 섬유소분해 활성이 우수한 균주를 동정하고, 효소의 생산조건, 효소의 분리 및 정제와 정제한 효소의 물리화학적 특성을 검토하였다. 분리된 미생물은 동정을 통하여 Pseudomonas sp. YD-15라고 명명하였고 avicel 1.2%, yeast extract 0.6%, $KNO_3$ 0.1%, $K_2HPO_4$ 0.2%, $MgSO_4$ $7H_2O$ 0.15%의 배지(pH 8.0)를 사용하여 $30^{\circ}C$에서 $60{\sim}80$시간 배양 하였을 때 최대 효소생산을 보였으며, 배양액으로부터 85% ammonium sulfate 침전, DEAE-Sepharose CL-6B chromatography, Sephadex G-100 gel filtration 과정을 통하여 14%의 수율로 정제도 15.3배의 효소단백질을 얻었다. 정제효소의 최적 작용 pH는 6.0 이었고 pH $5.0{\sim}8.0$ 사이에서 안정하였다. 최적 작용온도는$50^{\circ}C$였으며, $50^{\circ}C$까지는 안정하였다. 효소의 분자량은 약 100,000으로 추정되었으며, 기질인 carboxymethylcellulose(CMC)에 대한 Km 값은 40mg/ml 이었다.

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USNCAP에 대응하는 반응표면법을 이용한 조수석 안전구속장치 최적화 (Optimization of Passenger Safety Restraint System for USNCAP by Response Surface Methodology)

  • 오은경;이기선;손창규;김동석;채수원
    • 한국자동차공학회논문집
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    • 제22권6호
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    • pp.1-8
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    • 2014
  • Safety performance of a new car is evaluated through USNCAP and their results in the star rating are provided to the consumers. It is very important to obtain high score of USNCAP to appeal their performance to consumers. Therefore the car companies have made the effort to improve their car safety performance. These efforts should satisfy the demand not only to get high score but also to pass the FMVSS, NHTSA regulations on safety. Huge numbers of car crash tests have been conducted on these bases by car companies. However physical tests spend too much cost and time, as an alternative way, the simulation on the car crash could be a solution to reduce the cost and time. Therefore the simulations have been widely conducted in car industry and various researches on this have been reported. In this study, restraint system had been optimized to minimize the injury of female passenger. Belted $5^{th}%ile$ female frontal crash test was selected from various test methods of USNCAP for the study. Initial velocity of the test was 56km/h. The combination injury probability of USNCAP was selected as an objective function and the injury limit value, which was defined in FMVSS, was set to an optimization constraint. Many researches that were similar to this study had been conducted, however most of them had limitation that interaction between airbag and safety belt had not been considered. Contrary to these researches, the interaction was considered in this study.

남강 중권역의 효율적인 유역관리를 위한 중점관리 대상지류 선정 (Selection of Priority Management Target Tributary for Effective Watershed Management in Nam-River Mid-watershed)

  • 정강영;김경훈;이재운;이인정;윤종수;이경락;임태효
    • 한국물환경학회지
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    • 제29권4호
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    • pp.514-522
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    • 2013
  • The major 24 tributaries in Nam-River mid-watershed were monitored for discharge and water quality in order to understand the characteristics of the watershed and to select the tributary catchment for improving water quality. According to the analytical results of discharge and water quality monitoring data of 24 tributaries, the mean value of discharge below $0.1m^3/s$ was 62.5% among the monitored tributaries and it mostly exceeded the water quality standards of Nam-river mid-watershed ($BOD_5$ = 3 mg/L, T-P = 0.1 mg/L over). According to the stream grouping method and the water quality delivery load density ($kg/day/km^2$) based on the results of tributary discharge and water quality monitoring, the tributary watersheds for improving the water quality were selected. In the Nam-River mid-watershed, tributaries in the GaJwaCheon, HaChonCheon catchment (Group D, $BOD_5$ = 3 mg/L over) and in the UirYeongCheon, SeokGyoCheon catchment (Group A, T-P = 0.1 mg/L over), which have a small flow (and/or large flow) and a high concentrations of water pollutants. The various water quality improving scheme for tributaries, in accordance with the reduction of potential point source pollution by living sewage and livestock wastewater, should be established and implemented.

Developing drought stress index for monitoring Pinus densiflora diebacks in Korea

  • Cho, Nanghyun;Kim, Eunsook;Lim, Jong-Hwan;Seo, Bumsuk;Kang, Sinkyu
    • Journal of Ecology and Environment
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    • 제44권3호
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    • pp.115-125
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    • 2020
  • Background: The phenomenon of tree dieback in forest ecosystems around the world, which is known to be associated with high temperatures that occur simultaneously with drought, has received much attention. Korea is experiencing a rapid rise in temperature relative to other regions. Particularly in the growth of evergreen conifers, temperature increases in winter and spring can have great influence. In recent years, there have been reports of group dieback of Pinus densiflora trees in Korea, and many studies are being conducted to identify the causes. However, research on techniques to diagnose and monitor drought stress in forest ecosystems on local and regional scales has been lacking. Results: In this study, we developed and evaluated an index to identify drought and high-temperature vulnerability in Pinus densiflora forests. We found the Drought Stress Index (DSI) that we developed to be effective in generally assessing the drought-reactive physiology of trees. During 2001-2016, in Korea, we refined the index and produced DSI data from a 1 × 1-km unit grid spanning the entire country. We found that the DSI data correlated with the event data of Pinus densiflora mass dieback compiled in this study. The average DSI value at times of occurrence of Pinus densiflora group dieback was 0.6, which was notably higher than during times of nonoccurrence. Conclusions: Our combination of the Standard Precipitation Index and growing degree days evolved and short- and long-term effects into a new index by which we found meaningful results using dieback event data. Topographical and biological factors and climate data should be considered to improve the DSI. This study serves as the first step in developing an even more robust index to monitor the vulnerability of forest ecosystems in Korea.

도로경관의 시각적 특성 및 선호도에 관한 연구 -청주시 주진입로를 대상으로- (A Study on the Visual Character and Preference of Roadscape -In Case of the Main Entrance Road in Chongju-)

  • 정대영;심상렬;문석기
    • 한국조경학회지
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    • 제24권1호
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    • pp.15-31
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    • 1996
  • The purpose of this study was to figure out visual character and preference of roadscape for the main entrance road in Chongju. We took 9.6km which was from the Chongju interchange of Kyungbu expressway to Sangdang park and was thought to have a peculiar characteristics. This main entrance road was seperated into 4 parts according to arranging patterns of roadside trees and buildings. To investigate the visual characters, the sementic differential scale experiment was used. The visual characters, the sementic differential scale experiment was used. The visual preference was examined by analyzing visual volume of 4 factors. The results of the study based on these analyses were as below : 1. Factors that compose the visual characters of roadscape were classified by the emotional factor, the individual factor and the physical factor. These 3 factors showed a64.14% total variance. Among 3 factors the emotional factor which represented psychological reaction was appreciated to be the highest and the physical factor was assessed to be the lowest. 2. 24 items in total 14 adjectives showed the following ranking of mean values in sementic differential experiment : Road I -->RoadIII-->RoadII-->RoadIV. The mean values between Road I and RoadIV showed a significant difference, which can be explained to be a contrast between the natural factor and the artificial factor. 3. The mean value of the visual preference was the highest at Road I and the lowest at Road IV. While Road II and Road III showed 3.51 and 4.71, respectively. 4. The effect of 4 factors on visual preference was analyzed by regression as follows : Visual preference =1.2983+0.0627 + 0.0230+0.0203, R-Square=0.5

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최근 MODIS 식생지수 자료(2006-2008)를 이용한 동아시아 지역 지면피복 분류 (Land Cover Classification over East Asian Region Using Recent MODIS NDVI Data (2006-2008))

  • 강전호;서명석;곽종흠
    • 대기
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    • 제20권4호
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    • pp.415-426
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    • 2010
  • A Land cover map over East Asian region (Kongju national university Land Cover map: KLC) is classified by using support vector machine (SVM) and evaluated with ground truth data. The basic input data are the recent three years (2006-2008) of MODIS (MODerate Imaging Spectriradiometer) NDVI (normalized difference vegetation index) data. The spatial resolution and temporal frequency of MODIS NDVI are 1km and 16 days, respectively. To minimize the number of cloud contaminated pixels in the MODIS NDVI data, the maximum value composite is applied to the 16 days data. And correction of cloud contaminated pixels based on the spatiotemporal continuity assumption are applied to the monthly NDVI data. To reduce the dataset and improve the classification quality, 9 phenological data, such as, NDVI maximum, amplitude, average, and others, derived from the corrected monthly NDVI data. The 3 types of land cover maps (International Geosphere Biosphere Programme: IGBP, University of Maryland: UMd, and MODIS) were used to build up a "quasi" ground truth data set, which were composed of pixels where the three land cover maps classified as the same land cover type. The classification results show that the fractions of broadleaf trees and grasslands are greater, but those of the croplands and needleleaf trees are smaller compared to those of the IGBP or UMd. The validation results using in-situ observation database show that the percentages of pixels in agreement with the observations are 80%, 77%, 63%, 57% in MODIS, KLC, IGBP, UMd land cover data, respectively. The significant differences in land cover types among the MODIS, IGBP, UMd and KLC are mainly occurred at the southern China and Manchuria, where most of pixels are contaminated by cloud and snow during summer and winter, respectively. It shows that the quality of raw data is one of the most important factors in land cover classification.

물체 운반작업에 의한 산소맥의 변화 (Oxygen Pulse in Load Carrying)

  • 이병국;배달식;진항기;정규철
    • Journal of Preventive Medicine and Public Health
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    • 제5권1호
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    • pp.105-110
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    • 1972
  • On 6 healthy adult males aged 20-30 years, oxygen pulse was observed during carrying sand bags weighing 10, 20 and 30 kg on a level treadmill running with speeds of 3, 4 and 5 km/hr. The results obtained were as follows. 1. Oxygen pulse during load carrying on a level treadmill was proportionately increased with an increase of work load, average oxygen uptake per minute. Regression equation of oxygen pulse (y: $m\iota$/beat) on the average oxygen uptake (x:$\iota$/min) required for each grade of work was expressed as y=3.34x+5.99, $s_{y.x}=0.858$). 2. Oxygen pulse reached the highest value 2-5 minutes after the start of load carrying, and thereafter it decreased gradually to some extend as the load carrying was continued. Rate of decrease in the oxygen pulse was greater in lighter works. 3. In lighter works requiring less than $2.0\iota/min$ of oxygen uptake, no difference in oxygen pulse could be seen between methods of carrying even when the same amount of load was carried. In heavier works requiring more than $2.0\iota/min$ of oxygen uptake, oxygen pulse was larger in case of carrying a load by embrace, in both hands and on head than on back or on shoulder.

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High Resolution Probabilistic Quantitative Precipitation Forecasting in Korea

  • Oh, Jai-Ho;Kim, Ok-Yeon;Yi, Han-Se;Kim, Tae-Kuk
    • 한국제4기학회지
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    • 제19권2호
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    • pp.74-79
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    • 2005
  • Recently, several attempts have been made to provide reasonable information on unusual severe weather phenomena such as tolerant heavy rains and very wild typhoons. Quantitative precipitation forecasts and probabilistic quantitative precipitation forecasts (QPFs and PQPFs, respectively) might be one of the most promising methodologies for early warning on the flesh floods because those diagnostic precipitation models require less computational resources than fine-mesh full-dynamics non-hydrostatic mesoscale model. The diagnostic rainfall model used in this study is the named QPM(Quantitative Precipitation Model), which calculates the rainfall by considering the effect of small-scale topography which is not treated in the mesoscale model. We examine the capability of probabilistic diagnostic rainfall model in terms of how well represented the observed several rainfall events and what is the most optimistic resolution of the mesoscale model in which diagnostic rainfall model is nested. Also, we examine the integration time to provide reasonable fine-mesh rainfall information. When we apply this QPM directly to 27 km mesh meso-scale model (called as M27-Q3), it takes about 15 min. while it takes about 87 min. to get the same resolution precipitation information with full dynamic downscaling method (called M27-9-3). The quality of precipitation forecast by M27-Q3 is quite comparable with the results of M27-9-3 with reasonable threshold value for precipitation. Based on a series of examination we may conclude that the proosed QPM has a capability to provide fine-mesh rainfall information in terms of time and accuracy compared to full dynamical fine-mesh meso-scale model.

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MAGNETIC PROPERTIES OF INNER MAGNETOSPHERE DURING GEOMAGNETIC STORMS INFERRED FROM A TSYGANENKO MAGNETIC FIELD MODEL

  • Lee, D.Y.;Kim, K.C.;Choi, C.R.;Kim, H.J.
    • Journal of Astronomy and Space Sciences
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    • 제21권4호
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    • pp.303-314
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    • 2004
  • In this paper we report some properties of inner magnetospheric structure inferred from the T01_s code, one of the latest magnetospheric models by Tsyganenko. We have constructed three average storms representing moderate, strong, and severe intensity storms using 95 actual storms. The three storms are then modelled by the T01_s code to examine differences in magnetic structure among them. We find that the magnetic structure of intense storms is strikingly different from the normal structure. First, when the storm intensity is large, the field lines anchored at dayside longitudinal sectors become warped tailward to align to the solar wind direction. This is particularly so for the field lines anchored at the longitudinal sectors from postnoon through dusk. Also while for the moderate storm the equatorial magnetic field near geosynchronous altitude is found to be weakest near midnight sector, this depression region expands into even late afternoon sector during the severe storm. Accordingly the field line curvature radius at the equator in the premidnight geosynchronous region becomes unusually small, reaching down to a value less than 500 km. We attribute this strong depression and the dawn-dusk asymmetry to the combined effect from the enhanced tail current and the westward expansion/rotation of the partial ring current.

수중소음의 원격측정 (Telemetering of the Underwater Noise)

  • 신현옥;신형일
    • 수산해양기술연구
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    • 제23권1호
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    • pp.11-17
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    • 1987
  • 원격측정장치를 제작하여 그 성능을 조사하고, 정치망주변의 수중환경소음과 축양조내의 수중생물소음을 각각 직접.원격측정을 동시에 행하여 그 주파수특성을 비교 분석한 결과를 요약하면 다음과 같다. 1. 원격측정장치의 송신기의 입력신호전압을 100~300 mV로 하였을 때 수중환경소음의 원격측정치와 직접측정치는 50Hz~2KHz의 주파수범위에서 상관계수가 0.97로 가장 잘 일치하였다. 2. 원격측정한 수중환경소음의 음압준위는 송수신기간의 거리에 비례하여 감소하였다. 3. 축양조내의 수중생물소음을 원격측정하여 스펙트럼 분석한 결과 50Hz~2KHz의 주파수범위에서는 원격측정치와 직접측정치가 잘 일치하였으며, 2KHz 이상에서는 일치하지 않았다. 따라서, 원격측정장치의 송수신기의 총합주파수특성을 높이면 여러 가지 해양환경요소의 원격측정과 원력어군탐지기의 제작도 가능함을 알 수 있었다.

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