• Journal of Life Science
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• v.18 no.6
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• pp.845-851
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• 2008

${\beta}-Lactamase$, secreted from Bacillus sp. J105 strain was purified to a single band on SDS-PAGE by ammonium sulfate precipitation, ion exchange column chromatography and gel-filtration. The molecular weight of the purified enzyme was 31 kDa on SDS-PAGE and its isoelectric point was 7.35. Optimal pH and temperature for enzymatic reaction were 5 and $40^{\circ}C$, respectively. As a result of total amino acid composition analysis of the purified enzyme, Gly and Ala were occupied 14.1 and 13.3 mole %, respectively. Km and Vmax value of purified enzyme were 1.33 mM and 0.36 mM/ml using ampicillin as a substrate, respectively.

• Applied Biological Chemistry
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• v.35 no.4
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• pp.237-241
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• 1992

The protease from Halomonas sp. ES 10 was purified by methanol precipitation, gel filtration on Sephadex G-150 and G-200, and ion exchange chromatography on DEAE-Sephadex A-50. The purified enzyme was found to be homogeneous by polyacrylamide gel electrophoresis. The specific activity of purified enzyme was 1,014 units/mg protein, and the yield of the total activity from the culture filtrate was 7%. The optimal temperature and pH for the enzyme activity were $35^{\circ}C$, and pH 11.0, respectively. And the enzyme was stable in the range of $pH\;7.5{\sim}11.0$. The residual activity of the enzyme was 70%, when the enzyme was incubated at $50^{\circ}C$ for 40 min. The Km value of the enzyme was 7.4 mg/ml to milk casein. $Li^+$, $Ca^{2+}$, SDS and Tween 80 were appeared to activators, whereas $Hg^{2+}$ and EDTA to inhibitors. The addition of DFP and PMSF showed the relative enzyme activities of 63% and 107%, respectively, suggesting that the enzyme may not belong to serine type protease. When the alkaline protease was treated with 0.5 M and 1 M NaCl, the relative enzyme activities were 95% and 65%, respectively. This enzyme showed 20% and 15% higher enzyme activity than that of Aspergillus oryzae (Sigma Chemical Company product, P4755) in the presence of 0.5 M and 1 M NaCl.

• Journal of Preventive Medicine and Public Health
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• v.6 no.1
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• pp.27-41
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• 1973

In order to provide some basic data for the control of air and water pollution in Korea, the authors have estimated the amount of air and water pollutants emitted from industries which are employed over 20 employees. This study have done from July 1, 1972 to the end of March 1973. The results are as followings; 1. Total number of establishments with over 20 employees is 5,197 in Korea and the largest group establishments was the manufacturing of textiles with 1,363 establishments (26.2%). 2. By order of number of employees it was observed that there 2,800 industries with 20-59(53.9%) employees, 1,101 with 50-99 (21.2%), 571 with 100-199 (11.0%), 501 with 200-499 (9.6%) and 225 with over 500 (4.3%) respectively. 3. By order of regional distribution, it was observed that there were 2,257 industries in Seoul (43.2%) and 736 industries in Pusan(14.2%). 4. Industrial coal consumption was 596,154 M/T in 1972, but it' 11 be 315,000 M/T in 1980. Fuel consumption was 4,972,000 K1 in 1972, and estimated volume will be 19,370,000 K1 in 1980. 5. Total amounts of air polutants entitled from industries by fuel combustion were sulfur oxides 79,459 tons, carbon monoxide 33,908 tons, particulate 31,304 tons and hydrocarbon 30,280 tons in 1972 but in 1990 there will be sulfur oxides 1,010,474 tons, nitrogen oxides 204,575 tons, carbon monoxide 68,014 tons, particulate 64,820 tons and hydrocarbon 67,622 tons, respectively. 6. Annual emitted air pollutants through the working processes were sulfur oxides 91,250 tons and nitrogen oxides 32,485 tons in 1972, but sulfur oxides 118,625 tons and nitrogen oxides 42,555 tons will be present in 1980, respectively. 7. Annual emitted air pollutants by national unit area amounted to $0.77ton/km^2/year$ in 1965 and $14.7ton/km^2/year$ in 1980. 8. Total industrial wastes from all industries in Korea were estimated at 810,360 tons/day in 1972; manufacturing of chemicals and plastic products showed the highest amount of wastes at 470,000 tons/day. 9. The amounts of water pollutants due to industrial wastes were the B.O.D., 471.5 tons/day, suspended solid 331.5 tons/day, CN, 2.3 tons/day, and Cr. 3.4 tons/day in 1972, but it might be evident of a B.O.D. of 3,388 tons/day, suspended solid 2,544 tons/day, CN 20.1 tons/day, and 26.5 tone/day in 1990. 10. Total population equivalent of B.O.D. was 943,000 in 1972, and the estimated value in 1950 will be 6,780,000.

• International Journal of Highway Engineering
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• v.12 no.2
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• pp.9-16
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• 2010

Roughness is the most important factor to maintain the road performance, and affects greatly on the design life in Jointed Plain Concrete pavements. Also, the factors the evaluate pavement‘s commonality is the three method such as functionality, safety and structural performance. In evaluating function of road, representative factors is the roughness, which has been used to determine maintenance time as key standard. As research for roughness is absence in pavement design. Applied roughness-model had a low-reliability in Korea. Therefore, it is needed to develop reliable model in road roughness. In this research, uniform specific is applied to distribute them after selecting the concrete pavements. Concrete pavement is divided by sections of 238. Total length of this sections has 281km and account for 16% of total road length in korean concrete pavements for selected sections. Considering the korean roughness-model, the evaluation of roughness is performed for the freezing index, average annual rainfall, condition for the base, the amount of traffic as well as spalling(%), cracking(%), age(year) at the selected section at the selected section. Also, additional sections is selected to evaluate various age which affects on the roughness. As a result of the analysis, it showed that spalling(%), cracking(%), age(year), and the condition of the base affected road roughness. When the correlation with the road roughness was analyzed, the reliable model for road roughness was proposed, and the ratio that can explain road roughness was R2-68.8% and P value-0 which is statistically meaningful.

• Journal of Korea Water Resources Association
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• v.44 no.3
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• pp.169-177
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• 2011

The impacts of climate change on paddy irrigation water demands in Korea have been analyzed. High-resolution ($27{\times}27\;km$) climate data for the SRES A2 scenario produced by the Korean Meteorological Research Institute (METRI) and the observed baseline climatology dataset were used. The outputs from the ECHO-G GCM model were dynamically downscaled using the MM5 regional model by the METRI. The Geographic information system (GIS) was used to produce maps showing the spatial changes in irrigation water requirements for rice paddies. The results showed that the growing season mean temperature for future scenarios was projected to increase by $1.5^{\circ}C$ (2020s), $3.3^{\circ}C$ (2050s) and $5.3^{\circ}C$ (2080s) as compared with the baseline value (1971~2000). The growing season rainfall for future scenarios was projected to increase by 0.1% (2020s), 4.9% (2050s) and 19.3% (2080s). Assuming cropping area and farming practices remain unchanged, the total volumetric irrigation demand was projected to increase by 2.8% (2020s), 4.9% (2050s) and 4.5% (2080s). These projections are contrary to the previous study that used HadCM3 outputs and projected decreasing irrigation demand. The main reason for this discrepancy is the difference with the projected climate of the GCMs used. The temporal and spatial variations were large and should be considered in the irrigation water resource planning and management in the future.

• Applied Biological Chemistry
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• v.35 no.1
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• pp.23-29
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• 1992

Bromelain was purified from Korean pineapple, Ananas comosus, L. The enzyme was purified about 21 fold by DEAF-cellulose ion-exchange chromatography and gel filtration on Sephadex G-150. Purified enzyme was confirmed as active single band by polyacrylamide electrophoresis and the molecular weight was estimated to be about 22,000 by SDS-PAGE. The optimum pH and temperature were 6.0 and $60^{\circ}C$, respectively. The range of its stability to the pH and temperature were respectively 5.0 to 7.0 and below $50^{\circ}C$. It was found that $Mn^{2+}$ increased the enzyme activity, whereas $Mg^{2+}\;and\;Fe^{2+}$ decreased it abruptly. The purified enzyme was inhibited by p-chloromercuribenzoic acid, indicating that reactive SH groups are required for the enzyme activity. The reaction of the enzyme followed typical Michaelis-Menten kinetics with Km value of $5.747{\times}10^{-4}\;M\;and\;Vmax\;of\;131.58\;{\mu}g/min$ for casein. When meat was treated with the enzyme, free soluble nitrogen and amino acid nitrogen increased as enzyme concentration increased.

• Journal of Environmental Impact Assessment
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• v.23 no.5
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• pp.323-336
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• 2014

Using hedonic price method, this paper analyzes the impact of restriction for water quality protection on property value with the officially announced price of reference land in the city of Namyangju in 2012 to evaluate Water Use Fee, based on beneficiary pays principle, levied on the downstream area of the Han River in Korea. The results from the regression analyses of the models used show that the double-log model is the preferred model in the case of Namyangju. Using the double-log model, the total compensation for the city of Namyangju is estimated to be 8.6 trillion won with 95% confidence interval between 4.4 trillion and 12.4 trillion won. Under the perpetuity compensation scheme at the discount rate of 10%, the estimated annual compensation is 0.9 trillion won with 95% confidence interval between 0.4 trillion and 1.2 trillion won. This is more than Water Use Fee collected in 2012 for the Han River, which is approximately 0.5 trillion won. Considering the size of the restricted area of the Paldang area, which is more than 18 times of that of Namyangju, the rate of Water Use Fee, which is based on beneficiary pays principle and imposed on the residents of the downstream area, needs to be increased to sufficiently compensate the economic loss caused to the upstream areas of the Han River in Korea.

• Korean Journal of Food Science and Technology
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• v.15 no.4
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• pp.333-341
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• 1983

Tannase of Aspergillus sp. AN-11 isolated from contaminated acorns was purified by a procedure involving ammonium sulfate precipitation, DEAE-cellulose column chromatography and Sephadex G-200 gel filtration. Physiochemical properties of the purified tannase was investigated. Tannase was purified about 37 folds with the yield of 49% from the culture broth of Aspergillus sp. AN-11. The purified tannase was homogeneous on polyacrylamide gel disc electrophoresis and was dissociable into two identical subunits on SDS-polyacrylamide gel electrophoresis. The molecular weight of the tannase was determined to be 200,000 by gel filtration on Sephadex G-200. The purified tannase showed a typical protein ultraviolet spectrum. The enzyme had a optimum pH 5.5 and optimum temperature at 30 to $40^{\circ}C$. The enzyme was stable at a pH range from 5.0 to 6.5 and at the temperature below $30^{\circ}C$. The enzyme was inactivated remarkably by $CuCl_2$ and $ZnCl_2. The Km value of the enzyme was $7.58{\times}10^{-4}\;M$.

• Journal of Korean Society for Geospatial Information Science
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• v.16 no.1
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• pp.23-32
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• 2008

According to recent frequent local flash flood due to climate change, the very short-term rainfall forecast using remotely sensed rainfall like radar is necessary to establish. This research is to evaluate the feasibility of GIS-based distributed model coupled with radar rainfall, which can express temporal and spatial distribution, for multipurpose dam operation during flood season. $Vflo^{TM}$ model was used as physically based distributed hydrologic model. The study area was Yongdam dam basin ($930\;km^2$) and the 3 storm events of local convective rainfall in August 2005, and the typhoon.Ewiniar.and.Bilis.collected from Jindo radar was adopted for runoff simulation. Distributed rainfall consistent with hydrologic model grid resolution was generated by using K-RainVieux, pre-processor program for radar rainfall. The local bias correction for original radar rainfall shows reasonable results of which the percent error from the gauge observation is less than 2% and the bias value is $0.886{\sim}0.908$. The parameters for the $Vflo^{TM}$ were estimated from basic GIS data such as DEM, land cover and soil map. As a result of the 3 events of multiple peak hydrographs, the bias of total accumulated runoff and peak flow is less than 20%, which can provide a reasonable base for building operational real-time short-term rainfall-runoff forecast system.

• Microbiology and Biotechnology Letters
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• v.20 no.5
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• pp.551-558
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• 1992

The extracellular endoinulase from Streptomyces sp. 556 was purified and characterized, The culture broth was fractionated by ammonium sulfate saturation followed by DEAE-cellulose column chromatography and 5ephadex G-200 gel filtration, The ultimately purified fraction revealed a single band in 7.5% polyacrylamide gel electropherogram. The purified enzyme showed the maximal activity at pH 5.5-6.0 and $50^{\circ}C$, but lost 93% of inulase activity after 30 min incubation at $55^{\circ}C$ . The essen.tial amino acid residue for catalytic activity appeared to be tryptophan. This endo inulase was activated by $Mn^{2+}$, whereas inactivated by $Ag^{+}$, $Hg^{+}$, $Cu^{2+}$, $Zn^{2+}$, $Fe^{3+}$ and $Mo^{6+}$ EDTA and 8-hydroxyquinoline inhibited the enzyme so that the enzyme was considered to be a metalloenzyme. The Km value for inulin was 0.287 mM, and no invertase or $\alpha$-glucosidase activity was found in the enzyme.