• Title/Summary/Keyword: Km value

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Effect of pH on PAH Transport in Brush Border Basolateral Membrane Vesicles of Rabbit Proximal Tubule (가토 신장 근위세뇨관의 Brush Border 및 Basolateral Membrane Vesicle에서 PAH 이동에 미치는 pH의 영향)

  • Kim, Yong-Keun;Woo, Jae-Suk;Lee, Sang-Ho
    • The Korean Journal of Physiology
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    • v.22 no.2
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    • pp.281-293
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    • 1988
  • The effect of pH on the rate of PAH uptake was studied in rabbit renal basolateral membrane vesicles (BLMV) and brush border membrane vesicles (BBMV). In the absence of Na in incubation medium, a decrease in external $pH(pH_0)$ led to an increase in probenecid-sensitive PAH uptake by BLMV. In the presence of Na, the probenecid-sensitive PAH uptake was unaltered when the $pH_0$ decreased from 8.0 to 6.0 but further decrease in $pH_0$ to 5.5 increased significantly the uptake. The probenecid-sensitive PAH uptake was not affected by an alteration in pH per se in the absence of a pH gradient with or without the presence of Na. However, the presence of Na stimulated the probenecid-sensitive PAH uptake in all pH ranges tested over that measured in the absence of Na. A similar pattern of pH dependence on the PAH uptake was observed in BBMV but the presence of Na did not alter the probenecid-sensitive PAH uptake in the presence and absence of a pH gradient. Kinetic analysis for BLMV showed that Na or pH gradient increased Vmax of the probenecid-sensitive PAH uptake without a change in Km value. These results suggest that PAH is transported by $OH^-/PAH$ exchange process in the luminal membrane, but the pH dependence in the BLMV is not unequivocally consistent with an anion exchange process. The PAH transport is dependent on Na in BLMV but not in BBMV.

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A Study on the Temperature Change of Braking Disc and Thermal Conductivity during the Service (철도차량용 제동디스크의 운행중 온도 변화 및 열전도도 측정 연구)

  • Kim, Jae-Hoon;Goo, Byeong-Choon;Suk, Chang-Sung
    • Journal of the Korean Society for Railway
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    • v.10 no.6
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    • pp.665-669
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    • 2007
  • This study investigates the temperature change and thermal conductivity for the braking disc of the railway vehicle due to the types of train and service conditions. The temperature change was measured by non-contact Infrared thermometers. Average temperature was measured between $79.32^{\circ}C$ and $104.46^{\circ}C$ due to types of train and service section. In the same service section, the surface temperatures of Saemaul train were higher than Mugungwha train; the reason might have been the average service speed of Saemaul train (83km/h) was higher than Mugungwha train (107km/h), and the weight was similar 39t (Mugungwha) and 39.3t (Saemaul). But the maximum surface temperature was measured on the Mugungwha train; however the difference was not too big with the maximum temperature of Saemaul train. Also, the disc surface temperatures were changed due to the material of lining; metal and non-asbestos, on the same train and the same service section. In addition. the thermal conductivity was tested the thermal conductivities were increased by the increasing of the temperature. The change is too big between $100^{\circ}C$ and $200^{\circ}C$. But each average value is small. and the mechanical property change is very low. As a result, we conclude that this disc is suitable for usage between $100^{\circ}C$ and $300^{\circ}C$.

A Determination Model of the Data Transmission-Interval for Collecting Vehicular Information at WAVE-technology driven Highway by Simulation Method (모의실험을 이용한 WAVE기반 고속도로 차량정보 전송간격 결정 모델 연구)

  • Jang, Jeong-Ah;Cho, Han-Byeog;Kim, Hyon-Suk
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.9 no.4
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    • pp.1-12
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    • 2010
  • This paper deals with the transmission interval of vehicle data in smart highway where WAVE (Wireless Access for Vehicular Environments) systems have been installed for advanced road infrastructure. The vehicle data could be collected at every second, which is containing location information of the vehicle as well the vehicle speed, RPM, fuel consuming and safety data. The safety data such as DTC code, can be collected through OBD-II. These vehicle data can be used for valuable contents for processing and providing traffic information. In this paper, we propose a model to decide the collection interval of vehicle information in real time environment. This model can change the transmission interval along with special and time-variant traffic condition based on the 32 scenarios using microscopic traffic simulator, VISSIM. We have reviewed the transmission interval, communication transmission quantity and communication interval, tried to confirm about communication possibility and BPS, etc for each scenario. As results, in 2-lane from 1km highway segment, most appropriate transmission interval is 2 times over spatial basic segment considering to communication specification. In the future, if a variety of wireless technologies on the road is introduced, this paper considering not only traffic condition but also wireless network specification will be utilized the high value.

Geological and Geophysical Characteristics of the New Hebrides Basin (뉴헤브리디스 해분의 지질.지구물리학적 특징에 관한 연구)

  • Park, Chung-Hwa
    • Journal of the Korean earth science society
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    • v.18 no.6
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    • pp.559-564
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    • 1997
  • The New Hebrides Basin is an inactive non back-arc basin located at the convergent boundary of the Pacific and Info-Australian plates. This basin was formed from 46 Ma to 60 Ma. The basin has two spreading episodes with rates of 34 mm/a for 42 to 47 Ma and 17 mm/a for 47 to 60 Ma. The sediments covered in the basin has uniform thickness of 0.65 sec. The age-depth correlation curve of the New Hebrides Basin can be represented by the following equation: $Depth(m)=2689+312\sqrt{Age}(Ma)$ The coefficient of 312 in this equation is close to that for major oceans, 350. This suggests that the cooling processes of the lithospheres in the New Hebrides Basin and major oceans are similar to each other. Free-air gravity anomalies of the basin varying from -22.3 mgal to +59.0 mgal. The mean value is +30.2 mgal higher than those of the normal oceans. Moderately large free-air gravity anomalies in the New Hebrides Basin are presumably owing to its location on a marginal swell along the New Hebrides Trench. It is generally observed that the ocean floor is very gently uplifted in a zone about 200 km oceanward of the trench axis. Positive free-air gravity anomalies amounting to $50{\sim}60$ mgal are usually observed on the crest of the swell. This topography is presumably by bending of the oceanic lithosphere so as to dynamically maintain nonisostatic states for some duration.

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Detection of Clavibacter michiganensis subsp. michiganensis Assisted by Micro-Raman Spectroscopy under Laboratory Conditions

  • Perez, Moises Roberto Vallejo;Contreras, Hugo Ricardo Navarro;Herrera, Jesus A. Sosa;Avila, Jose Pablo Lara;Tobias, Hugo Magdaleno Ramirez;Martinez, Fernando Diaz-Barriga;Ramirez, Rogelio Flores;Vazquez, Angel Gabriel Rodriguez
    • The Plant Pathology Journal
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    • v.34 no.5
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    • pp.381-392
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    • 2018
  • Clavibacter michiganensis subsp. michiganesis (Cmm) is a quarantine-worthy pest in $M{\acute{e}}xico$. The implementation and validation of new technologies is necessary to reduce the time for bacterial detection in laboratory conditions and Raman spectroscopy is an ambitious technology that has all of the features needed to characterize and identify bacteria. Under controlled conditions a contagion process was induced with Cmm, the disease epidemiology was monitored. Micro-Raman spectroscopy ($532nm\;{\lambda}$ laser) technique was evaluated its performance at assisting on Cmm detection through its characteristic Raman spectrum fingerprint. Our experiment was conducted with tomato plants in a completely randomized block experimental design (13 plants ${\times}$ 4 rows). The Cmm infection was confirmed by 16S rDNA and plants showed symptoms from 48 to 72 h after inoculation, the evolution of the incidence and severity on plant population varied over time and it kept an aggregated spatial pattern. The contagion process reached 79% just 24 days after the epidemic was induced. Micro-Raman spectroscopy proved its speed, efficiency and usefulness as a non-destructive method for the preliminary detection of Cmm. Carotenoid specific bands with wavelengths at 1146 and $1510cm^{-1}$ were the distinguishable markers. Chemometric analyses showed the best performance by the implementation of PCA-LDA supervised classification algorithms applied over Raman spectrum data with 100% of performance in metrics of classifiers (sensitivity, specificity, accuracy, negative and positive predictive value) that allowed us to differentiate Cmm from other endophytic bacteria (Bacillus and Pantoea). The unsupervised KMeans algorithm showed good performance (100, 96, 98, 91 y 100%, respectively).

Performance evaluation by simulation for the angular luminous intensity distributions of marine lanterns using a tilting aspherical Fresnel lens and a C-8 type light bulb (기울어진 비구면 프레넬 렌즈와 C-8 type 전구를 이용한 해상용 랜턴의 배광곡선 시뮬레이션을 통한 성능평가)

  • Cho Hyun Seok;Jo Jae Heung;Park Seungl Nam;Park Chul Woung;Kim Yong Wan;Kim Jong Tae
    • Korean Journal of Optics and Photonics
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    • v.15 no.6
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    • pp.511-518
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    • 2004
  • Providing marine signal lanterns with advantages of little weight as well as large aperture, a Fresnel lens has been adopted to transfer the beam from the lanterns up to 10 nautical miles (18.53 km). A Fresnel lens with the diameter of 250 mm and 300 mm was designed by a lens design program and optimized by adjusting the groove parameters of the lens. The angular luminous intensity distribution (ALID) of this lens was calculated by using an illumination analysis program considering the ALID of a light bulb. At the best alignment of the bulb, the maximum luminous intensities (MLI) of the lantern were 1000 cd (in the case of 250 mm diameter) and 1300 cd (in the case of 300 mm diameter). These are more than the critical value of 720 cd that is the Korean Standard of MLI for the marine lantern. The ALID was investigated as a function of misalignment from the lens focus to determine the tolerance of the alignment ranges.

Resource Estimation of Actosity Gold Mineralized Belt, Uzbekistan (우즈베키스탄 악토시티 금광화대 자원량 평가)

  • Chi, Se-Jung;Park, Sung-Won;Kim, In-Joon;Heo, Chul-Ho
    • Economic and Environmental Geology
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    • v.47 no.2
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    • pp.169-180
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    • 2014
  • Surface geological and trench surveys and drilling exploration (total length, 1,100 m; 9 drill holes) were carried out to secure new Au ore bodies in the area($0.96km^2$) of Actosity gold field, where is located at the western Kuldjuktau mineralized district in the middle territory of Uzbekistan. Several Au ore bodies occurring as tabular or lens shapes with thickness of 0.5~35 m were newly discovered on the outcrops and extended to $N40{\sim}70^{\circ}\;W$ direction with dipping of $70{\sim}90^{\circ}$ SW or NE. Indicated ore resource of gold with Au grade of 0.25~3.52 mg/kg was newly estimated by 2,382 t(gold resource of 2.5 t) as a result of 9 drilling exploration in 2010~2012 from the Actosity gold field. Judging from the ore resources and Au grade of the Actosity gold field, economic potentiality of mining development seems to be low. Because of high possibility to secure new ore resources through more detailed exploration works from the Actosity area, the growth of econonic value will be expected by a mine of middle scale.

Observation of the Mesoscale Phenomena by Ocean Acoustic Tomography in the East Sea (동해에서 해양음향토모그래피에 의한 중규모 현상 관측)

  • Na, Jung-Yul;Han, Sang-Kyu;Lee, Jae-Hak;Shim, Tae-Bo;Kim, Kuh
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.4 no.3
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    • pp.170-179
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    • 1999
  • The SUS (Signal, Underwater Sound)-OAT experiment was carried out in the Ulleung Basin of the East Sea on 3 June 1997. The SUS-OAT system consisted of aircraft deployed shots as sources and a vertical line array (VLA) tethered by a receiver ship was used to survey a large area where a mesoscale warm eddy appears frequently. The experiment was carried out such that explosive charges set to detonate at 800 ft depth were dropped in a rectangular ($120{\times}120$ km). Sources were a rapidly deployable SUS charge (MK 61 MOD 0), and receiver is a fixed VLA, 90 m in length (150-240 m in receiver depth), composed of 10 elements equally spaced. The reference ray paths are computed by range-dependent acoustic model in canonical ocean based on the historical data. The singular value decomposition (SVD) method is used to obtain the horizontal perturbation of the temperature fields. Horizontal distributions of temperature fields at 150 m and 200 m depth show a weak warm eddy observed by AXBT and the inversely estimated temperature shows similar patterns in terms of the location of the warm eddy. In conclusion, the SUS-OAT experiment has been successful to estimate the position of warm eddy and its temperature field in the East Sea of Korea.

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Analysis of Impact Characteristics of Bonded Dissimilar Materials for Center Pillar (센터필라 적용을 위한 이종 접합강의 충격 특성 해석에 관한 연구)

  • Nam, Ki-Woo;Park, Sang-Hyun;Yoo, Jung-Su;Lee, Sang-Mun
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.36 no.8
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    • pp.929-934
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    • 2012
  • This study was carried out to analyze the dynamic characteristics of laser tailor-welded blanks (TWBs) made of dissimilar materials. The analysis was performed using Hyper Works 10.0 with Solver LS-DYNA v.971. 2D-Shell was used as the modeling element, and the number of elements and nodes was 35,641 and 36,561, respectively. The impact speed was 10 km/h. To analyze the impact characteristics according to the height of the weld line for the upper and lower parts of the center pillar, the length of the lower part was set as 300 and 400 mm. When the lower part was made of SPFC980 steel with a length of 300 mm, the deformation was the smallest and the absorbed energy of the impact force was the largest. On based the lower part of center pillar, the position of TWB shows the shorter and the better value. In other words, the performance depended on the proportion of the upper part made of high-strength SABC1470 steel. A lower part made of SPFH590 steel showed large deformation. In contrast, a lower part made of SPFC980 steel showed significantly lesser deformation. Therefore, the impact performance of a lower part made of SPFC980 steel with a length of 300 mm showed the best analysis result.

A Study on a utilizing Mobile Mapping System for establishing the High Speed Outdoor Positioning DB based on Field Check Data (정위치 기반 고속 실외 측위 DB 구축을 위한 MMS활용 방안에 관한 연구)

  • Lee, Ha Dong;Lee, Yun;Choi, Yun Soo;Jeong, In Hun
    • Journal of Korean Society for Geospatial Information Science
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    • v.25 no.2
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    • pp.31-37
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    • 2017
  • Recently, governmental authority and local government are looking for a method of utilizing location information of smart phone for urgent rescue in fire and kidnap situation. Under this background, in this study, a method of rapidly collecting, constructing location determination based Wi-Fi AP data utilizing location information of smart phone and mobile mapping system was suggested in order to construct precise positioning information that could be utilized under urgent situation. By performing compensation work for GPS/INS/DMI through collected outcome, position of collected vehicle was acquired. In addition, source data integrating Wi-Fi information and collected position by coupling based on Wi-Fi AP collector and GPS time was constructed and Wi-Fi radiomap was constructed by removing Wi-Fi signal noise that reduces precise position performance. As a result of performing location determination performance assess ment by selecting 10 test positions by each local government, result value of 25.46cm for total local government average and 27.76m for SD could be obtained. It is considered that this result could be utilized as a technology of being able to supplement or substituting GPS location determination technology that is impossible in plocation determination of mobile communication company's base station (200m~2km) and indoor being used at present.