• Title/Summary/Keyword: Klebsiella pnemoniae

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Antibacterial Activity of Activated Carbon Fibers Containing Copper Metal (구리 함유 활성 탄소 섬유의 항균 특성)

  • 박수진;김병주;이종문
    • Polymer(Korea)
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    • v.27 no.3
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    • pp.235-241
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    • 2003
  • The polyacrylonitrile (PAN)-based activated carbon fibers (ACFs) containing copper metal were electrolytically prepared in introducing the antibacterial activity into ACFs. The antibacterial activity was investigated by dilution test against Staphylococous aureus (S. aureus; gram positive and virulence) and Klebsiella pnemoniae (K. pnumoniae: gram negative and avirulence). The micropore and textural properties of the ACFs containing copper metal were characterized by BET, t-plot, and H-K methods. The ACFs showed slight decreases in BET's specific surface area, micropore volume, and total pore volume as copper metal increased. However, the antibacterial activities of the ACFs were strongly increased against S. aureus as well as K. pnumoniae, which could be attributed to the presence of copper metal in CU/ACFs systems.

bla Genotype and Molecular Epidemiological Analysis of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Chungcheong Regional Hospitals (충청지역병원에서 분리된 Extended-Spectrum β-Lactamase 생성 대장균과 폐렴간균의 bla 유전형 및 분자역학적 분석)

  • Yook, Keun Dol;Yang, Byoung Seon;Park, Jin Sook
    • Korean Journal of Microbiology
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    • v.50 no.2
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    • pp.114-118
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    • 2014
  • A total of 122 ESBL-producing intestinal bacteria were collected from regional hospitals in the Chungcheong area. Combination disk test (CDT) was performed for antimaicrobial susceptability using cefotaxime and cefotaxime/clavulanate according to Clinical Laboratory Standard Institute (CLSI). Mutiplex PCR using specific primers was performed for a detection of ESBL-genotypes and enterobacterial repetitive intergenic consensus (ERIC)-PCR was carried out for the tracking of molecular epidemiology. In the confirmation test using CDT, 73 out of 76 (96.1%) ESBL-producing Escherichia coli and 43 out of 46 (93.4%) ESBL-producing Klebsiella pnemoniae were positive. In the multiplex PCR, 60.5% of E. coil were positive for CTX-M-2 type gene and 56.5% of K. pneumoniae were positive for VEB -1 type gene. In the ERIC-PCR, E. coil isolates formed 5 clusters and K. pneumoniae isolates were grouped into 4 clusters depending on region. Genotypes of clinical isolates are useful for detection and differentiation of ESBL producing intestinal bacteria. The ERIC-PCR method is thought to be helpful for establishing a regional surveillance system for infection due to its formation of different clusters depending on region.

Antibacterial Activity of Activated Carbon Fibers Containing Silver Metal

  • Park, Soo-Jin;Kim, Byung-Joo;Ryu, Seung-Kon
    • Carbon letters
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    • v.4 no.3
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    • pp.140-145
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    • 2003
  • Antibacterial behaviors of PAN-based activated carbon fibers (ACFs) containing silver metal were investigated. The effects of surface and pore structures of the ACFs were studied by $N_2$/77 K adsorption and D-R plot as a function of silver loading content. The antibacterial activities were investigated by a dilution test against Staphylococcus aureus (S. aureus; gram positive) and Klebsiella pnemoniae (K. pnumoniae; gram negative). As experimental results, the ACFs showed some decreases in specific surface areas, micropore volumes, and total pore volume with an increase of silver content. However, the antibacterial activities of the ACFs were strongly increased against S. aureus as well as K. pnumoniae, which could be attributed to the presence of antibacterial metal in the ACFs system.

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Distribution and antimicrobial susceptibility patterns of bacteria isolated from genital tract of riding mares (승용 씨암말의 생식기 유래 세균의 분포 및 항생제 감수성 양상)

  • Cho, Young-Jae;Lee, Yong-Duck;Jang, Jong-Duck;Shin, Kwang-Hyeu;Park, Yong-Soo;Yang, Jae-Hyuk;Kim, Sung-Joon;Cho, Gil-Jae
    • Korean Journal of Veterinary Service
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    • v.38 no.1
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    • pp.19-23
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    • 2015
  • This study was carried out to investigate the genital tract bacterial flora of riding mare in Jangsu stud farm during March to September, 2014. The specimens were collected from vaginal and uterus using a swab from 104 riding mares. Colonies were selected on blood and MacConkey agar plates, and identified as standard biochemical properties and Maldi-Tof MS. From this study, we isolated 148 strains including Escherichia (E.) coli (14.19%), Streptococcus (S.) equi subsp. zooepidemicus (2.7%), Streptococcus (S.) dysgalactiae subsp. equisimilis (2.03%), Klebsiella (K.) pneumonia (1.35%) and other strains from riding mares. In antimicrobial agents susceptibility test, it showed a high sensibility to the antibiotics of the most. E. coli and S. zooepidemicus were visible to have a high sensibility to almost antibiotics used in this study. However, K. pnemoniae showed a high antibiotic resistance patterns. These results may provide the basic information to establish strategies for the treatment and prevention of reproductive diseases in riding mares in Korea.

A Study on Screeining of Antibacterial Oriental Medicines Against Pulmonary Disease-causing Bacteria (폐렴(肺炎) 유발균(誘發菌)의 생육(生育)을 억제(抑制)하는 한약재(韓藥材) 탐색(探索)에 관(關)한 연구(硏究))

  • Jeong, Byoung-Woon;Seo, Woon-Gyo;Jeong, Ji-Cheon;Han, Young-Hwan
    • The Journal of Dong Guk Oriental Medicine
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    • v.7 no.2
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    • pp.121-140
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    • 1999
  • The various oriental herbal medicines, which have usually been used for treatment of reducing fever, purging intense heat and detoxication, were screened to determine the antibacterial activity and the minimal inhibitory concentration against pulmonary disease-causing Klebsiella pneumoniae, Streptococcus pyogenes, and Streptococcus pneumoniae. The results obtained were as follows: 1. Among the 23 oriental medicines tested, the water-soluble extracts of Coptis japonica, Scutellaria baicalensis and Picrorrhiza kurrooa showed the antibacterial activity against K.pneumoniae and that of C. japonica against S. pyogenes. The antibacterial activities of C. japonica, Prunusmume, Schizandra chinesis, Scutellaria baicalensis were also found against S.pneumoniae. When C.japonica was used, the high antibacterial activity was shown against Bacillus subtilis and other extracts showed a little activity against B. subtilis and E. coli as a control. 2. The ethanol-soluble extracts of Patrinia scabriosaefolia, P. mume, S. baicalensis, S. chinesis showed the antibacterial activity against K. pneumoniae and those of S. baicalensis, C. japonica, S. chinesis, P. mume agaist S. pyogenes and S. pnuemoniae. However, those extract showed a little antibacterial activity against B. subtilis and E. coli except for that the extract of C. japonica showed comparatively high growth inhibition of B. subtilis. 3. Among the medicinal herbs tested, the water and ethanol extrats of C. japonica showed very extcellent antibacterial activity against the pathogenic bacteria and controls. 4. When the water-soluble extracts of C. japonica and S. baicalensis, minimal inhibitory concentrations (MICs) against K. pneumoniae were $10mg/m{\ell}$ and $22 mg/m{\ell}$, respectively. The MICs of the ethanol-soluble extracts of P. mume and P. scabriosaefolia were $5mg/m{\ell}$ and $20mg/m{\ell}$, respectively. 5. For the MICs against S.pyogenes, C. japonica showed $15mg/m{\ell}$ with the water-soluble extract and P. mume and C. japonica with the ethanol-souble extract did $5mg/m{\ell}$ and $10mg/m{\ell}$, respectively. 6. For the MICs against S. pneumoniae, C. japonica and P. mume with the water- and ethanol-souble extract showed $5mg/m{\ell}$ and $10mg/m{\ell}$, respectively. As a result, the highest antibacterial activity was found in the water- and ethanol-soluble extracts of C. japonica against pulmonary disease-causing bacteria, K. pneumoniae, S. pyogenes. and S. pnuemoniae. Also, the water- and ethanol-soluble extracts of S.chinesis. P.mume, S.baicalensis, and P.kurrooa showed hight antibacterial activities.

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