• 대한방사선방어학회:학술대회논문집
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• 2009.11a
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• pp.214-215
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• 2009

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2008.04a
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• pp.157-158
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• 2008

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2012.10a
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• pp.357-358
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• 2012

• Genomics & Informatics
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• v.3 no.4
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• pp.149-153
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• 2005

Asthma is an inflammatory airways disease characterized by bronchial hyperresponsiveness and airways obstruction, which results from a complex interaction of genetic and environmental factors. Interleukin (IL)-13 and IL-4 are important in IgE synthesis and allergic inflammation, therefore genes encoding IL-13 and IL-4 are candidates for predisposition to asthma. In the present study, we screened single-nucleotide polymorphisms (SNPs) in IL-13 and IL-4 and examined whether they are risk factors for asthma. We resequenced all exons and the promoter region in 12 asthma patients and 12 normal controls, and identified 18 SNPs including 2 novel SNPs. The linkage disequilibrium(LD) pattern was evaluated with 16 common SNPs, and haplotypes were also estimated within the block. Although IL-13 and IL-4 are localized within 27 kb on chromosome 5q31 and share many biological profiles, this region was partitioned into 2 blocks. One SNP and three SNPs were determined as haplotype-taggingSNPs (htSNPs) within IL-13 and IL-4 haplotype-block, respectively. No significant associations were observed between any of the SNPs or haplotypes and development of asthma in small number of Korean subjects. However, the genetic variants of IL-13 and IL-4 would provide valuable strategies for the genotyping studies in large population.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2001.11a
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• pp.279-280
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• 2001

중국이나 몽고에서 발생한 황사는 수천 km를 이동하여 한국, 일본 그리고 멀리 태평양건너 북미대륙까지 수송될 수 있는 것으로 보고되고 있다. 우리나라에서 관측되는 황사는 주로 고비사막이나 중국황하유역의 건조지대에서 발생한 먼지바람의 영향을 받는 것으로 파악된다. 황사는 장거리수송되는 과정에 대류권 화학 메카니즘을 변화시키고, 광범위한 영역에 걸쳐 복사체계를 변화시키며 또한 시정장애 등의 불편을 초래하고 있다 (Gao et al., 1992). (중략)

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2003.06a
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• pp.144-144
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• 2003

• Journal of Microbiology and Biotechnology
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• v.18 no.9
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• pp.1606-1612
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• 2008

Interleukin (IL)-32 is a recently identified proinflammatory cytokine that is one of the IL-18 inducible genes, and plays an important role in autoimmune and inflammatory diseases. We produced antibodies against IL-32 and studied the expression of IL-32 in human stomach cancer. We detected IL-32 secreted from K-562 cells which were stably transfected with IL-32 and in the sera of stomach cancer patients by a sandwich ELISA using a monoclonal antibody KU32-52 and a polyclonal antibody. In order to optimize a sandwich immunoassay, recombinant IL-32a was added, followed by the addition of a biotinylated KU32-52 into microtiter plate wells precoated with a goat anti-IL-32 antibody. The bound biotinylated KU32-52 was probed with a streptavidin conjugated to HRP. This sandwich ELISA was highly specific and had a minimal detection limit of 80 pg/ml (mean${\pm}$SD of zero calibrator) and measuring up to 3,000 pg/ml. This ELISA showed no cross-reaction with other cytokines such as hIL-1$\alpha$, hIL-1$\beta$, hIL-2, hIL-6, hIL-8, hIL-10, hIL-18, and hTNF-$\alpha$. Intra-assay coefficients of variation were 18.5% to 4.6% (n=10), and inter-assay coefficients were 23% to 9% (n=10). The average IL-32 level in the sera of 16 stomach cancer patients (189 pg/ml) was higher than that of 12 healthy control men (109 pg/ml). Our results indicate that serum IL-32 level can be detected by using an established ELISA, and that this immunoassay and mAb KU32-09 specific for immunohistochemistry can be used in the detection of expressed and secreted IL-32 in stomach cancer patients.

• Proceedings of the Materials Research Society of Korea Conference
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• 2008.11a
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• pp.33.1-33.1
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• 2008

• Proceedings of the Korean Magnestics Society Conference
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• 2006.06a
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• pp.52-53
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• 2006

• Proceedings of the Korean Magnestics Society Conference
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• 2006.06a
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• pp.78-79
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• 2006