• Toxicological Research
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• 제14권2호
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• pp.151-156
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• 1998

Acetaminophen (APAP) and gentamicin are widely used for many patients, but little in-formation is available regarding the combined effects of APAP and gentamicin. This study was aimed to investigate the potent nephrotoxicity following combined-treatment with APAP and gentamicin. Serum biochemical parameters and histopathological changes in the kidney were observed in female SD rats after continuous daily treatment with either 600 mg/kg/day APAP, and/or 300 mg/kg/day gentamicin for 3 days, and compared with saline sham-treated control animals. APAP and gentamicin combination-treated rats exhibited inconsistent increasing tendency in blood urea nitrogen (BUN) by 96 hours after the last treatment, compared to control or the animals treated with each drug. The relative kidney weights were also increased. Histopathological findings of kidneys revealed that necrosis of proximal convoluted tubules were higher in rats treated with APAP and gentamicin combination than the rats treated with each drug alone. These results suggest that combination use of both drugs have more severe nephrotoxicity than treating each drug alone.

• 동아시아식생활학회지
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• 제20권4호
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• pp.575-581
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• 2010

강낭콩의 비율을 달리하여 제조한 탁주의 이화학적 물성변화를 검토한 결과, 다음과 같은 결과를 얻었다. 총산의 경우, $T_{-5}$에서 산도가 0.70%로 가장 높았고, $T_{-6}$에서 0.50%로 가장 낮은 결과를 보여주고 있다(p<0.05). pH는 발효 종료 후 $T_{-1}$에서 3.82에서부터, $T_{-2}$에서 4.19로 주세법상 탁주 pH 범위인 3.8~4.7에서 벗어나지 않았다(p<0.05). 환원당의 경우, 발효 2일째 전체적으로 환원당 함량이 $T_{-1}$ 4.96에서 2.00%로 가장 많이 감소하는 경향을 보이고 있으며, 발효 종료 후 환원당 함량의 분포를 살펴보면 $T_{-1}$ 0.49에서부터 $T_{-7}$ 0.59%의 분포를 보여주고 있고(p<0.05), 발효 6일째부터 12일째까지 완만한 변화를 보였다. 알코올 생산량은 발효 직후 $T_{-1}$에서 17.43%로 가장 높게 나타났고, $T_{-2}$에서 13.83%로 가장 낮았다(p<0.05). 항산화 효과는 $T_{-1}$ 40.0%, $T_{-2}$ 57.0%, $T_{-3}$ 50.1%, $T_{-4}$ 47.0%, $T_{-5}$ 47.1%, $T_{-6}$ 46.0% 그리고 $T_{-7}$ 45.0%의 항산화 활성을 보이고 있으며, 강낭콩 함량이 높은 시료에서 항산화 활성이 높게 나타나는 것을 확인할 수 있었다. 관능검사 측정 결과, 단맛, 신맛, 조직감 그리고 기호도를 평가한 결과, 각 시료간의 유의차를 확인할 수 있었다(p<0.05). 강낭콩으로 담금한 탁주가 백미로 담금한 탁주에 비해 조직감과 선호도에서 좋은 결과를 보이고 있어 새로운 제품으로서의 가능성을 확인할 수 있었다.

• Clinical and Experimental Pediatrics
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• 제63권4호
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• pp.151-156
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• 2020

Background: Acute kidney injury (AKI) is one of the most significant postoperative complications of pediatric cardiac surgery. Because serum creatinine has limitations as a diagnostic marker of AKI, new biomarkers including neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), and interleukin-18 (IL-18) are being evaluated to overcome these limitations and detect AKI at an early stage after cardiac surgery. Purpose: This study aimed to investigate the clinical usefulness of these biomarkers in young children. Methods: Thirty patients with congenital heart diseases who underwent cardiac surgery using cardiopulmonary bypass (CPB) were selected, and their urine and blood samples were collected at baseline and 6, 24, and 48 hours after surgery. Serum creatinine and blood urea nitrogen levels as well as NGAL, KIM-1, and IL-18 levels in urine samples were measured, and clinical parameters were evaluated. Results: Of the 30 patients, 12 developed AKI within 48 hours after cardiac surgery. In the AKI group, 8 of 12 (66.6%) met AKI criteria after 24 hours, and urine KIM-1/creatinine (Cr) level (with adjustment of urine creatinine) peaked at 24 hours with significant difference from baseline level. Additionally, urine KIM-1/Cr level in the AKI group was significantly higher than in the non-AKI group at 6 hours. However, urine NGAL/Cr and IL-18/Cr levels showed no specific trend with time for 48 hours after cardiac surgery. Conclusion: It is suggested that urine KIM-1/Cr concentration could be considered a good biomarker for early AKI prediction after open cardiac surgery using CPB in young children with congenital heart diseases.

• Journal of Ginseng Research
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• 제37권2호
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• pp.187-193
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• 2013

The effect of Korean red ginseng (KRG) on diabetic renal damage was investigated using streptozotocin (STZ)-induced diabetic rats. The diabetic rats showed loss of body weight gain, and increases in kidney weight and urine volume, whereas the oral administration of KRG at a dose of 100 or 250 mg/kg of body weight per day for 28 d prevented these diabetes-induced physiological abnormalities. Among the kidney function parameters, elevated plasma levels of urea nitrogen and creatinine in diabetic control rats tended to be lowered in KRG-treated rats. In addition, administration of KRG at a dose of 100 mg/kg body weight in the diabetic rats showed significant decreases in serum glucose and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), implying that KRG might prevent the pathogenesis of diabetic complications caused by impaired glucose metabolism and oxidative stress. KRG also significantly reduced advanced glycation end product (AGE) formation and secretion from kidney of diabetic rats. Furthermore, KRG decreased the levels of N-(carboxymethyl) lysine and expression of AGE receptor. KRG also reduced the overexpression of cyclooxygenase-2 and inducible nitric oxide synthase in the kidney via deactivation of nuclear factor-kappa B. We also found that KRG prevented STZ-induced destruction of glomerular structure and significantly suppressed high glucose-induced fibronectin production. Taken together, KRG ameliorates abnormalities associated with diabetic nephropathy through suppression of inflammatory pathways activated by TNF-${\alpha}$ and AGEs. These findings indicate that KRG has a beneficial effect on pathological conditions associated with diabetic nephropathy.

• 생명과학회지
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• 제11권1호
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• pp.70-75
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• 2001

Effect of Quartz porphyry(QP) on clinical chemical parameters of serum in male white java sparrow by feeding the basal diet(Control group) containing 3.0% QP(QP group) for 14 days was studied. Body weight gain, feed intake, the weight of the gizzard and the heart were not significantly different in the both groups. However, the weights of kidney and liver in the QP group were higher than in the control group. The contents of triglyceride, cholesterol, total protein, glucose, creatinine, blood urea nitrogen, uric acid and albumin, and the activities of glutamic oxaloacetic transaminase(GOT) and glutamic pyruvic transaminase(GPT) in serum were not significantly different in the both groups. The activity of alkaline phosphatase(ALP) in serum was higher in the QP group than in the control group.

• 한국응용곤충학회지
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• 제59권2호
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• pp.153-163
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• 2020

차응애(Tetranychus kanzawai Kishida)의 기주식물에 대한 가치를 평가하기 위하여 기주식물로 제주 감귤의 주요 품종인 한라봉, 감귤원에서 많이 서식하는 잡초인 왜제비꽃과 대조군으로 강낭콩을 선정하였다. 항온조건 20℃, 25℃, 30℃에서 발육실험과 산란실험을 진행하고 생명표 분석을 수행하였다. 차응애는 한라봉 어린잎, 왜제비꽃, 강낭콩에서 발육을 완료했지만 한라봉 구엽에서는 발육을 완료하지 못했다. 한라봉 어린잎을 섭식하는 차응애의 총 발육기간은 각 온도조건에서 17.4, 13.4, 10.2일로 강낭콩에서 조사된 16.1, 9.5, 7.0일보다 길었다. 한라봉 어린잎에서의 차응애 성충의 수명은 각 온도에서 19.1, 15.0, 12.3일로 강낭콩에서 조사된 22.1, 14.1, 10.9일과 유의한 차이는 없었으며, 산란수는 각 온도에서 18.1, 23.9, 17.8개로 왜제비꽃(93.3, 105.8, 115.0개)과 강낭콩(127.4, 121.0, 117.1개)에서 보다 적었다. 조사된 발육데이터를 기반으로 생명표 통계량을 추정한 결과 서로 간 유의한 차이를 보였으며 내적자연증가율(r_m)은 각 온도에서 강낭콩(0.1542, 0.2563, 0.3251), 왜제비꽃(0.1087, 0.2007, 0.2673), 한라봉(0.0868, 0.1002, 0.1217)순으로 높게 나타났다. 이상의 결과를 종합하여 감귤원(한라봉)의 차응애 관리 전략에 대하여 고찰하였다.

• 한국환경성돌연변이발암원학회지
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• 제22권4호
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• pp.324-330
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• 2002

Bisphenol A (BPA) is a monomer widely used in the manufacturing polycarbonate plastics or epoxy resin, and xenobiotics recently known as endocrine disrupting chemical. In this paper, to assess the effects of bisphenol A on immunopathological parameters (body weight, organ weight, hematological parameters, cellularity and surface marker) in mice, ICR female mice were orally exposed to BPA dissolved in olive oil as concentrations of 100, 500, 1000 ㎎/㎏/day b.w. 5 days a week for 30 days (subacute exposure). Liver - and kidney weight was significantly increased as dose-dependent manner, but body- , spleen- and thymus- weight didn't changed. In hematological parameters, WBC and MCHC were lowered but HCT and MCV were siginificantly enhanced. There was no significant differences in peritoneal macrophages number of the mice exposed to BPA. However, number of splenocytes of spleen, CD3/sup +/ and CD4/sup +/ cell in splenocytes, CD4/sup +/ and CD8/sup +/ cell in thymocytes were decreased at the mice subacutely exposed to BPA. In addition, BPA decreased expression of B7-1 and B7-2 on macrophages. Therefore, these results showed BPA may affect hematological parameters, cellularity and surface marker of immunocytes.

• 한국방사선학회논문지
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• 제9권4호
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• pp.227-234
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• 2015

본 연구는 간 초음파영상에서 통계적 속성 기반의 밝기 히스토그램에 기초한 픽셀 질감분석 파라미터(평균밝기, 왜곡도, 균일도, 엔트로피)와 간과 콩팥실질의 밝기 차를 이용한 영상분석을 통해 미만성 간질환의 컴퓨터보조진단 적용 가능성을 알아보고자 하였다. 실험은 간 초음파영상(정상, 지방간, 간경화)에서 관심영역($50{\times}50$픽셀)을 설정하고 4가지의 픽셀 질감분석 파라미터와 간과 콩팥의 실질 밝기의 차를 이용하여 질환인식률을 평가하였다. 그 결과 평균밝기, 균일도, 엔트로피의 질환인식률은 100%, 왜곡도 96%로 높게 나타났으며, 간과 콩팥의 실질 밝기 차는 정상 $-1.129{\pm}12.410$, 지방간 $33.182{\pm}11.826$으로 뚜렷한 차이를 나타내었으나, 간경화의 경우 $-1.668{\pm}10.081$로 정상과는 다소 작은 차이를 나타내었다. 이러한 결과를 바탕으로 높은 질환인식률을 보인 픽셀 질감분석 파라미터와 실질 밝기 차를 이용한 컴퓨터보조진단은 미만성 간질환의 감별에 유용한 도구로써 임상적인 활용 가능성이 있으며, 판독 오류를 최소화하고 정확한 진단과 치료방향 제시에 도움이 될 것으로 기대된다.

• Childhood Kidney Diseases
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• 제10권2호
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• pp.142-151
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• 2006

Purpose : Growth retardation is one of the serious problems in children with nephropathy requiring long-term steroid therapy. We observed the efficacy and safety of recombinant human growth hormone(rhGH) on the growth in children with long-term steroid therapy. Methods : We studied 60 children(male 47, female 13) with nephropathy who received rhGH(1 U/kg/week) for more than 0.5 years($1.39{\pm}1.12$). Their mean age was 11.0 years($11.17{\pm}2.62$). They received steroid therapy from January 1987 through July 2005, and the mean duration of steroid therapy was $4.32{\pm}2.97$ years. Among the patients, there were 32 nephrotic syndrome, 9 IgA nephropathy, 4 mesangial proliferative glomerulonephritis, 4 focal segmental glomerulosclerosis, 2 Henoch $Sch\ddot{o}nlein$ nephritis, 2 Alport syndrome and 7 other cases. Data were gathered on the growth parameters, such as growth velocity, height standard deviation score(SDS), IGF-1, IGFBP-3, bone mass density(BMD) and general chemistry changes. Results : Height velocity increased significantly with rhGH therapy from $3.29{\pm}1.95$ to $8.66{\pm}3.75$(cm/yr) and height SDS decreased from $-0.72{\pm}0.93$ to $-1.04{\pm}0.86$ at one year after steroid therapy but increased to $-0.55{\pm}0.96$ at one year after rhGH administration(P<0.05). BMD improved from $0.71{\pm}0.14$ to $0.79{\pm}0.15g/cm^2$(P<0.05). IGF-1 increased from $445.09{\pm}138.01$ to $506.62{\pm}181.31ng/mL$(P<0.05). IGFBP-3 decreased from $4073.75{\pm}700.78$ to $3933.61{\pm}789.25ug/L$ numerically, but there was no statistically significant difference(P=0.533). Conclusion : The administration of rhGH in the short stature patients who received long-term steroid therapy showed improvement in growth parameters such as SDS, growth velocity, and BMD without significant side-effects or changes in the biochemical parameters.

• 대한임상검사과학회지
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• 제53권3호
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• pp.208-216
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• 2021

코로나바이러스 감염증 19는 중증급성호흡기증후군 코로나바이러스 2의 명칭에서 명명되었다. 이 연구는 코로나바이러스 감염증 19 환자의 신기능 상태를 조사하는 것을 목적으로 하였다. 본 연구는 경기도 남부 한 병원에서 코로나바이러스 감염증 19로 입원한 649명 환자를 대상으로 하였다. 이번 연구에서 검토한 코로나바이러스 감염증 19 환자의 혈액요소질소와 크레아티닌은 증가된 패턴을 보였다. 본 연구의 대상자 중 남성의 혈액요소질소는 정상 범위보다 높았다. 특히 60대, 80대 이상에서 높게 나타났고, 크레아티닌 수치도 비슷하게 나타났다. 이 연구에서 환자의 전해질 검사는 정상 범위에서 벗어나는 결과는 관찰되지 않았다. 이 연구의 대상에서 80세 이상의 남성은 60 mL/min/1.73 m² 이하의 만성신장질환 역학협력 사구체여과율을 나타냈다. 최근 연구에 따르면 COVID-19의 일부 심각한 사례는 코로나바이러스에 감염되기 전에 기저 신기능의 문제가 없었던 사람들에게도 콩팥 손상의 징후를 보이고 있다. 따라서 여러 지표 평가의 신기능 검사는 검사결과의 검토는 코로나19 환자의 비정상 신기능 발견에 도움이 될 수 있다.