• Title/Summary/Keyword: Ki-ho School

Search Result 1,681, Processing Time 0.036 seconds

Proteomic Analysis of Toxoplasma gondii KI-1 Tachyzoites

  • Choi, Si-Hwan;Kim, Tae-Yun;Park, Sung-Goo;Cha, Guang-Ho;Shin, Dae-Whan;Chai, Jong-Yil;Lee, Young-Ha
    • Parasites, Hosts and Diseases
    • /
    • v.48 no.3
    • /
    • pp.195-201
    • /
    • 2010

  • We studied on the proteomic characteristics of Toxoplasma gondii KI-1 tachyzoites which were originally isolated from a Korean patient, and compared with those of the well-known virulent RH strain using 2-dimensional electrophoresis (2-DE), mass spectrometry, and quantitative real-time PCR. Two-dimensional separation of the total proteins isolated from KI-1 tachyzoites revealed up to 150 spots, of which 121 were consistent with those of RH tachyzoites. Of the remaining 29 spots, 14 showed greater than 5-fold difference in density between the KI-1 and RH tachyzoites at a pH of 5.0-8.0. Among the 14 spots, 5 from the KI-1 isolate and 7 from the RH strain were identified using MALDI-TOF mass spectrometry and database searches. The spots from the KI-1 tachyzoties were dense granule proteins (GRA 2,3,6, and 7), hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGRPTase), and uracil phosphoribosyltransferase (UPRTase). The spots from the RH strain were surface antigen 1 (SAG 1), L-lactate dehydrogenase (LDH), actin, chorismate synthase, peroximal catalase, hexokinase, bifunctional dihydrofolate reductase-thymidylate synthase (DHTR-TS), and nucleosidetriphosphatases (NTPases). Quantitative real-time PCR supported our mass spectrometric results by showing the elevated expression of the genes encoding GRA 2,3, and 6 and UPRTase in the KI-1 tachyzoites and those encoding GRA 7, SAG 1, NTPase, and chorismate synthase in the RH tachyzoites. These observations demonstrate that the protein compositions of KI-1 and RH tachyzoites are similar but differential protein expression is involved in virulence.

  • https://doi.org/10.3347/kjp.2010.48.3.195 인용 PDF KSCI

Discharge delay reduction by controlling the roughness of dielectric surface in AC PDP

  • Seo, Ki-Ho;Whang, Ki-Woong
    • 한국정보디스플레이학회:학술대회논문집
    • /
    • 2006.08a
    • /
    • pp.1491-1494
    • /
    • 2006

  • We report a method for reducing the address discharge delay. The address discharge delay was reduced when the MgO protective layer was made on the dielectric area which was made rough intentionally. The delay reduction was more pronounced in the formative delay.

  • PDF

Equalizing pulse with error diffusion technique using Look-up-Table and subfield pattern for reducing dynamic false contour

  • Seo, Ki-Ho;Whang, Ki-Woong
    • 한국정보디스플레이학회:학술대회논문집
    • /
    • 2003.07a
    • /
    • pp.321-324
    • /
    • 2003

  • We reported the method for the relatively simple equalizing pulse to reduce dynamic false contour. Equalizing pulse is determined by look-up-table and moving direction and velocity of the image. Used lookup-table is a few. If image moves to right or left, necessary LUT is only one for the velocity of 1 pixel/tvfield to right or left. This technique makes the process simple after obtaining motion information.

  • PDF