• 대한본초학회지
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• 제30권6호
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• pp.69-75
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• 2015

Objectives : This study was designed to investigate effects of the combination with Korean Red Ginseng (Panax ginseng C.A. Meyer), Gastrodia Rhizoma (Gastrodia elata Blume) and Polygoni Multiflori Radix (Polygonum multiflorum Thunberg) on metabolic disorders including cholesterol and erectile dysfunction in hyperlipidemia rats.Methods : Animals were divided into six groups; Control with normal diet, high fat/cholesterol-diet (HFCD), fluvastatin, Korean Red Ginseng treated (KRG), and the combination treated (Korean Red Ginseng, Gastrodia Rhizoma and Polygoni Multiflori Radix; 1:1:1 for KGP1 and 2:1:1 for KGP2). The experimental groups initially received HFCD for 10 weeks and then treated orally with fluvastatin, KRG, KGP1 and KGP2 during the final 6 weeks. Erectile function was determined by the measurements of intracavernosal pressure (ICP) and maximal arterial pressure (MAP) after electrical stimulation of the cavernosal nerve.Results : KGP2 decreased the level of total cholesterol and LDL cholesterol in the sera of HFCD rats without no changes of body weights. KRG, KGP1 and KGP2 decreased the level of C-reactive protein (CRP) levels except of fluvastatin, synthetic HMG-CoA reductase inhibitor. KRG, KGP1 and KGP2 significantly increased the ICP, ICP/MAP ratio, area under the curve (AUC) compared with those of normal rat. Morphometric analyses showed that KRG, KGP1 and KGP2 increased the volume of smooth muscle and the regular arrangement of collagen fibers in corpus cavernosum of HFCD rats. The penile expression of eNOS was increased by KRG, KGP1 and KGP2.Conclusions : Based on these results, we suggest that the combination with Korean Red Ginseng, Gastrodia Rhizoma and Polygoni Multiflori may improve hyperlipidemia through regulating the lipid profiles and erectile dysfunction in rats.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.719-732
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• 2003

A kojic acid derivative, kojic acid 7-O-$\beta$-D-tetraacetylglucopyranoside(KTG) was synthesized. Regio-and stereo-selective glycosylation at 7-postion in kojic acid with $\beta$-D-pentaacetylglucose was achieved with high yield(80％) by the use of Lewis acid and organic base in nonpolar solvent. KTG was hydrolyzed in methanol by the aid of sodium methoxide to give kojic acid 7-O-$\beta$-D-glucopyranoside(KGP). KGP is freely soluble in water and soluble in methanol and ethanol. Its structure was comfirmed by $^1$H-NMR and $^{13}$ C-NMR. Tyrosinase activity inhibition of KGP was measured with mushroom tyrosinase compared with ascorbic acid, kojic acid and arbutin. KGP showed higher tyrosinase inhibition activity($IC_{50}$/=33.3 $\mu\textrm{g}$/ml) than ascorbic acid(63.2 $\mu\textrm{g}$/ml) and arbutin(91.8 $\mu\textrm{g}$/ml) but lower inhibition activity than kojic acid(8.3 $\mu\textrm{g}$/ml). To test free-radical scavenging activity, we used 1, 1-diphenyl-2-picrylhydrazyl(DPPH) as a free-radical source. Free-radical scavenging activity of KGP was very low($SC_{50}$/>1000 $\mu\textrm{g}$/ml) compared with ascorbic acid($SC_{50}$/=2.68 $\mu\textrm{g}$/ml) and arbutin($SC_{50}$/=180$\mu\textrm{g}$/ml). Melanin formation inhibition of KGP was measured in B16 melanoma, compared with kojic acid, arbutin and Vitamin C. Inhibition activity of KGP for melanin formation was not found within test concentrations.

• 수산해양기술연구
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• 제37권3호
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• pp.181-189
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• 2001

본 논문은 조업 어선의 정확한 조업 위치를 실시간으로 수록할 수 있게 하고, 저렴하며 효율적인 어선조업정보 자동기록장비 개발을 위해 GPS 모듈(KGP9800C, KiRyung)의 측위 정도에 대한 실험을 육상과 해상의 기준점에서 행하였으며, 또한 측위 정도의 검정을 위해 동일한 기준점에서 DGPS 수신기(MGP-100D, Shin-A)와 DGPS 비콘(GP-36, Furuno)으로써 측정하여 비교하였다 또한. CPS 모듈의 오차를 최소화시키기 위한 알고리즘을 사용하여 측위오차를 개선하고, 그 측위 정도를 분석 검토하였다. 그 결과 수정 전의 CPS 모듈의 측위 정도는 육상에서의 경우. 확률원 반경 12.0m, 해상의 경우 36.0m로서 기준점으로 북동쪽으로 약 400.0m 편위하였으나 측위오차 최소화 알고리즘에 의해 구한 편위오차는 10.0m 이내였다. 또 한, DCPS 수신기 및 DGPS 비콘의 측위 정도와 비교 검토한 결과 육상과 해상에서 모두 거의 일치하였다. 따라서 본 실험의 결과 실험용 GPS 모듈 (KGP9800C)은 한 개의 CPU보드(V25)를 사용하여 실시간으로 신뢰성있는 조업 어선의 위치정보를 제공할 수 있음을 확인할 수 있었다.

• 대한화장품학회지
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• 제30권3호
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• pp.409-414
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• 2004

코직산은 티로시나제의 억제에 의한 미백효능을 가지는 물질로 널리 알려져 있으나, 낮은 안정성으로 인하여 화장품 원료로의 사용에 제약을 갖고 있다. 이에 본 연구에서는 유기합성적 방법에 의해 안정성과 미백효과를 가지는 코직산 유도체를 고수율로 합성하였다. $O-pentaacetyl-{\beta}-D-glucose를$ 루이스산과 유기염기를 이용하여 위치 선택적이고, 입체 선택적으로 코직산의 6번 위치에 도입시켜 $kojic\;acid\;6-O-2',3',4',6'-tetraacetyl-{\beta}-D-glucopyranoside\;(KTGP, 80{\%})를$ 합성한 후, 가수분해하여 kojic acid 6-0-f-D-glucopyranoside $(KGP, 70\%)를$ 수득하였다. $^1H-NMR과\;^{13}C-NMR$ 분석으로 구조를 확인하였다. KGP를 가지고 티로시나제 활성저해, 프리라디칼소거능과 멜라닌합성저해 실험을 실시하였고, 그 결과 티로시나제 활성저해와 프리라디칼소거능에서 코직산보다 높거나 비슷한 활성을 보여주었다. 코직산 수준의 미백효능을 확인하였기에 유기 화학적으로 합성된 KGP의 미백원료로서 사용을 기대할 수 있다.

• 대한본초학회지
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• 제31권4호
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• pp.71-77
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• 2016

Objectives : Adipogenesis was defined as a differentiation process of preadipocytes into the adipocytes. Thus, to control of this process can be one of the most important strategies to prevent obesity. Korean ginseng(Panax ginseng C.A. Meyer) is one of the most widely used medicinal herbs. Although multiple biological activities of Korean ginseng, particularly ginsenosides, have been known, the anti-adipogenic role and function of polysaccharides from Korean ginseng are still unclear. In this study, we examined anti-adipogenic activity of polysaccharides and its molecular basis mechanisms are further investigated.Methods : The cytotoxicity of KGP in 3T3-L1 was evaluated by MTT assay. Anti-adipogenic effect of KGP was examined by Oil Red O (ORO) staining and microscopy observation in 3T3-L1 mature adipocytes. The mRNA expression levels of adipogenic transcriptional factors were analyzed by reverse transcription-polymer chain reaction (RT-PCR). To elucidate the adipogenic molecular mechanism of KGT, SB431542 (TGF-β specific inhibitor) was used.Results : We found that polysaccharides showed no effect on the viability of 3T3-L1 preadipocytes. Dose dependent inhibitory effect of polysaccharides on 3T3-L1 adipogenesis was observed as judged by ORO staining and microscopic image analysis. To obtain further mechanistic insight into anti-adipogenic function of polysaccharides, we then tested the effect of polysaccharides treatment on the adipogenic marker genes. The mRNA expressions level of C/EBPα, PPARγ, C/EBPβ, and fatty acid synthase (FAS) were dose-dependently inhibited by KGP treatment in 3T3-L1 mature adipocytes.Conclusions : In conclusion, these findings suggest that the KGP could be used in treatment of obesity and overweight related diseases.

• Animal Bioscience
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• 제35권8호
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• pp.1121-1128
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• 2022

Objective: This study aimed to identify the genetic diversity and population structure of Mongolian horse populations according to the province of residence (Khentii, KTP; Uvs, USP; Omnogovi and Dundgovi, GOP; Khovsgol, KGP) using 14 microsatellite (MS) markers. Methods: A total of 269 whole blood samples were obtained from the four populations (KTP, USP, GOP, KGP) geographically distinct provinces. Multiplex polymerase chain reaction (PCR) was conducted using 14 MS markers (AHT4, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG6, HTG7, and VHL20), as recommended by the International Society for Animal Genetics. Capillary electrophoresis was conducted using the amplified PCR products, alleles were determined. Alleles were used for statistical analysis of genetic variability, Nei's DA genetic distance, principal coordinate analysis (PCoA), factorial corresponding analysis (FCA), and population structure. Results: On average, the number of alleles, expected heterozygosity (H_Exp), observed heterozygosity (H_Obs), and polymorphic information content among all populations were 11.43, 0.772, 0.757, and 0.737, respectively. In the PCoA and FCA, GOP, and KGP were genetically distinct from other populations, and the KTP and USP showed a close relationship. The two clusters identified using Nei's DA genetic distance analysis and population structure highlighted the presence of structurally clear genetic separation. Conclusion: Overall, the results of this study suggest that genetic diversity between KTP and USP was low, and that between GOP and KGP was high. It is thought that these results will help in the effective preservation and improvement of Mongolian horses through genetic diversity analysis and phylogenetic relationships.

• 한국식품위생안전성학회지
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• 제38권6호
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• pp.544-550
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• 2023

치주질환의 원인균인 Porphyromonas gingivlais (P. gingivalis)의 병인인자중 하나인 gingipain 효소에 대한 억제활성을 갖는 유산균을 개발하고자 본 연구실에 보관중인 30여종의 유산균 배양상등액의 활성을 검색하였다. 그 결과 발효식품에서 분리, 동정한 Weisiella cibaria SPM402와 Lactobacillus paracasei SMP412가 P. gingivalis에 대한 항균효과, gingipain 유전자 발현억제 및 gingipain 효소활성 억제능이 확인되었다. W. cibaria SPM402 의 배양 상등액 WC402는 10 mg/mL에서 kgp 유전자는 0.71±0.02배 발현되었고, rgpB 유전자는 0.71±0.14배 발현되 약간의 발현억제 효과가 확인되었다. 반면 L. paracasei SMP412의 배양상등액 LP412는 10 mg/mL 농도에서 kgp 유전자는 0.19±0.08배, rgpA 유전자는 0.09±0.02배, rgpB 유전자는 0.24±0.03배 발현되어 LP412의 뛰어난 gingipain 유전자 발현억제 효과를 확인하였다. 10 mg/mL농도에서, Kgp 효소 활성은 WC 402에 의해 78.65±3.58% (CAG), 82.45±1.22% (CFG), LP412에 의해 80.71±2.11% (CAG), 85.81±0.05% (CFG) 억제되었다. 동일한 농도에서 Rgp 활성은 WC402에 의해 78.6±1.01% (CAG), 86.78±0.47% (CFG), LP412에 의해 82.93 ±1.26% (CAG), 88.81±0.36% (CFG) 억제되었다. 이상의 결과들을 통해 WC402와 LP412는 P. gingivalis에 대한 항균효과 뿐 아니라 병인인자 gingipain을 효과적으로 억제하는 기능성 유산균임을 확인하였다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제15권12호
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• pp.4508-4530
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• 2021

Today distributed data storage service are being widely used. However lack of proper means of security makes the user data vulnerable. In this work, we propose a Randomized Block Size (RBS) model for secure data storage in distributed environments. The model work with multifold block sizes encrypted with the Chinese Remainder Theorem-based RSA (C-RSA) technique for end-to-end security of multimedia data. The proposed RBS model has a key generation phase (KGP) for constructing asymmetric keys, and a rand generation phase (RGP) for applying optimal asymmetric encryption padding (OAEP) to the original message. The experimental results obtained with text and image files show that the post encryption file size is not much affected, and data is efficiently encrypted while storing at the distributed storage server (DSS). The parameters such as ciphertext size, encryption time, and throughput have been considered for performance evaluation, whereas statistical analysis like similarity measurement, correlation coefficient, histogram, and entropy analysis uses to check image pixels deviation. The number of pixels change rate (NPCR) and unified averaged changed intensity (UACI) were used to check the strength of the proposed encryption technique. The proposed model is robust with high resilience against eavesdropping, insider attack, and chosen-plaintext attack.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2001년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.47-48
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• 2001

국제해양법 발효 이후로 한반도 주변의 해양에 관련된 정세는 급격한 변화를 가져오게 되었으며, 1998년 한ㆍ일 어업협정이 재 체결되고, 최근 한ㆍ중어업협정도 체결됨에 따라 우리나라 연ㆍ근해어업정보에 대한 효율적인 관리체계가 구축되지 않으면 안될 형편에 처하게 되었다. TAC제도의 실시가 본격화됨에 따라 어선어업과 관련된 정보의 데이터베이스화와 실질적인 관리체계의 확립이 절실히 요구되고 있으며, 이를 위해서는 우선 어선조업정보 자동기록장비를 개발하여 각 조업어선의 정확한 조업위치의 추적과 어획량의 정량적인 파악이 이루어져야 한다. (중략)

• Genomics & Informatics
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• 제17권4호
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• pp.40.1-40.9
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• 2019

While studies aimed at detecting and analyzing indels or single nucleotide polymorphisms within human genomic sequences have been actively conducted, studies on detecting long insertions/deletions are not easy to orchestrate. For the last 10 years, the availability of long read data of human genomes from PacBio or Nanopore platforms has increased, which makes it easier to detect long insertions/deletions. However, because long read data have a critical disadvantage due to their relatively high cost, many next generation sequencing data are produced mainly by short read sequencing machines. Here, we constructed programs to detect so-called unmapped regions (UMRs, where no reads are mapped on the reference genome), scanned 40 Korean genomes to select UMR long deletion candidates, and compared the candidates with the long deletion break points within the genomes available from the 1000 Genomes Project (1KGP). An average of about 36,000 UMRs were found in the 40 Korean genomes tested, 284 UMRs were common across the 40 genomes, and a total of 37,943 UMRs were found. Compared with the 74,045 break points provided by the 1KGP, 30,698 UMRs overlapped. As the number of compared samples increased from 1 to 40, the number of UMRs that overlapped with the break points also increased. This eventually reached a peak of 80.9% of the total UMRs found in this study. As the total number of overlapped UMRs could probably grow to encompass 74,045 break points with the inclusion of more Korean genomes, this approach could be practically useful for studies on long deletions utilizing short read data.