• Korean Journal of Poultry Science
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• v.29 no.2
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• pp.109-116
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• 2002

The objective of this study was to investigate the effect of feeding organic acid mixture and yeast culture on the Performance and e99 quality of laying hens fur sixteen weeks. Four hundred and fifty 19-wk-old ISA Brown layers were alloted to five treatments with five replications of each. The supplemental levels of organic acid mixture(OAM) and yeast culture(YC) in the experimental diets were 0(control), OAM 0.1, 0.2%, and YC 0.1, 0.2%, respectively. Corn -Soy basal diet containing 16.0% CP and ME 2,770 kca1/kg ME. Eggs were collected and weighed every day. Eg3 Production, fred intake and fled conversion were recorded in every four weeks. Fatty acid composition of e99 Yolk, e99she11 breaking strength, thickness and Haugh unit were measured a(her every eight weeks. Egg Production and daily egg mass of birds fed 0.2% OAM and 0.1% YC tended to be higher than those of the other treatment groups. Egg weight was the highest in hens fed with 0.2% OAM treatment, but was not significantly different. Feed conversion of hens in all OAM and YC treatments was lower than those of control, but was not statistically different. Eggshell breaking strength of hens on OAM treatments tended to be higher than those of YC treatment and control. Haugh units of the birds fed OAM was significantly higher than controls (P<0.05). 599 yolk color score of OAM treatment was higher than those of YC treatments and control, but the difference was not significant. At 27-wk-old, arachidonnic acid content in eggs from the birds fed 0.1% OAM diet was significantly higher(P<0.05) than controls. The results of this experiment indicated that dietary organic acid mixture and yeast culture tended to improve the egg Production and internal egg quality.

• Korean Journal of Poultry Science
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• v.29 no.2
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• pp.81-88
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• 2002

The objective of this study was to investigate the line-by-feed additive interaction on egg traits and yolk cholesterol level in laying hens. Hy-line W-98 (white shell line) and Hy-line Brown (brown shell line) were used as experimental stocks and three groups were assigned in each line. The age of hens used in this study were from 40- to 46-wk-old. Two different levels of chitosan were also used as additive in chicken diet. The first (CH$_{30}$) and second (CH$_{60}$) experimental groups were fed basal diet added 30mg and 60mg chitosan/kg feed, respectively. And the control group (CON) hens were fed with basal diet only. The eggs were collected and analyzed at the end of 40, 42, 44, and 46-wk-old. In the mean egg weight, the CH$_{30}$ in Hy-line Brown was significantly heavier (P<0.05) than CON, but the CH$_{30}$ in Hy-line W-98 was significantly lighter (P<0.05) than CON. Whereas, the mean egg weight did not significantly differ between CH$_{60}$ and CON in both line. The mean albumen percentage in Hy-line Brown did not show significant between experimental groups and control group. On the other hand, the mean albumen percentage of CH$_{60}$ in Hy-line W-98 was significantly lower (P < 0.05) than that of CON, but there was no significantly different between CH$_{30}$ and CON. In the mean yolk percentage, it showed a reverse trend as shown in the mean albumen percentage; there were significantly higher (P<0.05) in CH$_{60}$ than in CON fur the Hy-line W-98, and higher in CH$_{30}$ than in CON for the Hy-line Brown. However, the mean shell percentages did not significantly differ between experimental groups and control group in both lines. In the mean yolk cholesterol levels, the CH$_{30}$ and CH$_{60}$ in both lines were lower by 0.46 mg ∼2.80 mg per gram yolk than CON. Although the interaction effect of line-by-supplementing level of chitosan in hens diet on the pooled mean of yolk cholesterol level was no significant, it showed a highly significant interaction (P<0.01) at three of four experimental ages.

• Journal of Chest Surgery
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• v.31 no.9
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• pp.827-836
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• 1998

Background: Effective cardiopulmonary resuscitation (CPR) should provide acceptable hemodynamics for the vital organs during cardiac arrest and early restoration of spontaneous circulation that guarantees long-term, neurologically intact survival. CPR using heart-lung bypass has been suggested as an option for that use. This study was designed to determine the effectiveness of standard CPR techniques, closed-vs. open-chest CPR, which could be used in the future study verifying the role of heart-lung bypass CPR. Material and Method: By using adult mongrel dogs, closed-chest CPR (CCCPR, n=4) and open-chest CPR (OCCPR, n=5) were compared with respects to hemodynamics, restoration of spontaneous circulation(ROSC), and survival. Ventricular fibrillation-cardiac arrest (VF-CA) was induced by electrical shock in all animals. After 4 minutes of cardiac arrest, basic life support (BLS) was applied for 15 minutes and followed by advanced life support (ALS). ALS was maintained until achi ving ROSC but not longer than 30 minutes regardless of the recovery. Resuscitation procedures in either group were standardized by adopting the protocol of American Heart Association. Result: Prearrest baseline hemodynamic data was not different between two groups. During resuscitation, substantially higher systolic pressure was maintained in OCCPR group than in CCCPR group (45$\pm$15 vs. 33$\pm$11 mmHg during BLS, 83$\pm$36 vs. 44$\pm$15 mmHg during ALS; p=NS). Mean pulmonary arterial pressure went up to the level of mean systemic arterial pressures in CCCPR group and to half of that in OCCPR group, and had kept higher in CCCPR group throughout CPR (32$\pm$10 vs. 22$\pm$4 mmHg during BLS and 32$\pm$15 vs. 24$\pm$10 mmHg during ALS; p=NS). ROSC was obtained in 4 of 5 dogs receiving open-chest CPR and 2 of 4 closed-chest CPR. Prolonged survival was noted in all dogs in OCCPR group (6 to 1440 hours) but not in CCPR group (p<.05). Conclusion: These findings indicate that open-chest CPR can be more effective t maintain hemodynamics during cardiac arrest and to obtain restoration of spontaneous circulation and survival. Further experiment will be designed to compare heart-lung bypass CPR with open-chest CPR.

• Journal of Chest Surgery
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• v.35 no.6
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• pp.407-419
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• 2002

Atherosclerosis is a chronic inflammatory disease of the arterial wall characterized by progressive accumulation of lipids, cells, and extracellular matrix. Matrix metalloproteinases(MMPs) and tissue inhibitor of metalloproteinases(TIMPS) contribute to vascular matrix remodeling in atherosclerosis, and some cytokines may play role in the synthesis or activation of MMPs or TIMPs. Material and Method： We produced experimental atherosclerotic plaques in 9 rabbits by atherogenic hypercholesterol diet for 12 weeks, and 10 other rabbits were used as control group with standard laboratory chow, At that time, 19 rabbits were sacrificed and aorta, coronary arteries and blood specimens were prepared. The expressions of MMP-9, TIMP-2 and interleukin(IL)-18, and the bioactivity of IL-6 were investigated with H&E stain, immunohistochemical stain, immunoblotting(Western blot analysis), and bioassay. Result： Serum cholesterol in the experimental group increased up to 1258$\pm$262 mg/dL(control group： 41$\pm$7 mg/dL). All experimental group showed well-developed atherosclerotic plaques in aorta and coronary artery. The expression of MMP-9 in aorta and coronary artery of the experimental group showed significant increase than that of the control group by immunohistochemistry. Among the experimental group, complicated lesions with intimal rupture or complete luminal occlusion, demonstrated stronger expression of MMP-9. Interestingly, there was no difference in expression of TIMP-2 between the experimental and the control group. These findings were confirmed by Western blot analysis. The bioassay revealed significant up-regulation of serum bioactivity of IL-6 in the experimental group(4819.60$\pm$2021.25 IU/$m\ell$) compared to that of IL-6 in the control group(27.20 $\pm$ 12.19 IU/$m\ell$). IL-18 was expressed in all atherosclerotic plaques, whereas little or no expression was detected in the control group. Conclusion： The increased MMP-9 expression along with the unchanged TIMP-2 expression seem to be contributory factors in extracellular matrix degradation in atherosclerosis. Focal overexpression of MMP-9 may promote plaque destabilization and cause complications of atherosclerotic plaques such as thrombosis with/without acute coronary syndrome. Elevation of IL-6 and IL-18 may be more than just markers of atherosclerosis but actual participants in lesion development. Identification of critical regulatory pathway is important to improve the understanding of the cellular and molecular basis of atherosclerosis and may open the way for novel therapeutic strategies.

• Journal of Chest Surgery
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• v.40 no.1 s.270
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• pp.32-36
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• 2007

Background: Although acute renal failure (ARF) after coronary artery bypass graft (CABG) is relatively rare, but devastating complication with high mortality. Our study aims to evaluate the effectiveness of early application of CRRT in patients with ARF which developed after on-pump CABG. Material and Method: Two hundred and eighty seven patients underwent isolated on-pump CABG between May 2002 and Feb. 2006 at our institution, of whom 15 (5.2%) needed CRRT (11 patients for postoperatively developed ARF and the remaining 4 patients with preexisting dialysis-dependent chronic renal failure (CRF) for postoperative hemodynamic and metabolic control). Criteria for early application of CRRT were as follows; decreased urine output less than 0.5cc/h/kg for 2 consecutive hours and elevated serum creatinine level greater than 2.0 mg/dL. Result: The incidence of ARF requiring CRRT after on-pump CABG was 3.9% (11/283) and the overall hospital mortality of patient with CRRT was 33.3% (5/15). Of 5 deaths, 4 were patients with postoperatively developed ARF, and 1 was a patient with pre-existing dialysis-dependent CRF patient. The mean time between the operation and the initiation of CRRT was $25.8{\pm}5.8$ hours and the mean duration of CRRT was $62.1{\pm}41.2$ hours. Of the 7 survivors who were not on dialysis-dependent preoperatively, 6 patients fully recovered renal function during hospital stay and 1 patient required permanent renal supportive treatment after discharge from hospital. Conclusion: Early application of CRRT could maintain stable postoperative hemodynamic status and make outcomes better than those of previous reports in patients with ARF which developed after on-pump CABG.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.2
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• pp.100-108
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• 2000

High sodium contents in food-waste compost(FWC) is the greatest limitation to recycle it to arable lands in Korea. The effects of the FWC application to paddy soil on the growth of rice plants, cationic balance in plants, and the sodicity of soil have been studied in pot trials. The effects of FWC application were compared with those of NaCl compound and swine manure compost(SMC) application. $Na_2O$ contents of FWC were high as 2.2%. Immediately after transplanting, rice plants in three treatments showed severe wilting in the order of 40Mg FWC $ha^{-1}$ > NPK+900kg $NaClha^{-1}$ > 20Mg FWC $ha^{-1}$. The high EC value and volatile acid contents of soil solution were regarded as the cause of severe wilting of young rice plants. Increase of NaCl application rate upto $900kgha^{-1}$ showed no significant reduction of dry matter yield at harvesting stage. Regardless of application rates FWC reduced the dry matter yield at harvesting stage, while SMC increased it with increase of application rates upto $40Mgha^{-1}$. In NPK+NaCl and FWC treatments, Na contents and equivalent ratio in plants increased linearly with increase of Na application rates. Between Na and K equivalent ratio negative correlation with high significance was shown. In contrast to much difference of Na, K, and Na/K equivalent ratio among treatments, little difference of Na+K indicated the physiological substitution of Na for K in rice plants. Na use efficiency in NPK+NaCl and FWC treatments showed 12-22%.

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.5
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• pp.253-258
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• 2006

An empirical model of nitrous oxide emission from agricultural soil has been applied. It is based on the relationship between $N_2O$ and three soil parameters, soil mineral N(ammonium plus nitrate) content in the topsoil(0-15cm), soil water-field pore space, and soil temperature, determined in a study on clay loam and sandy loam at the pepper field in 2004. For comparisons between estimated and observed values of $N_2O$ emissions in the pepper field, it was investigated that $N_2O$ amount in the clay loam and sandy loam were overestimated as 12.2% and less estimated as 30%, respectively. However, $N_2O$ emissions were overestimated as 27.1% in the clay loam and 14.7% in the sandy loam from $N_2O$ gas samples collected once a week at the same time analyzing soil parameters. This modelling approach, based as it is well established and widely used soil measurements, has the potential to provide flux estimates from a much wider range of agricultural sites than would be possible by direct measurement of $N_2O$ emissions.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.2
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• pp.103-111
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• 2008

This study was carried out to investigate the optimal application rate and time of mixed expeller cake (MEC) for the replacement of chemical fertilizer. Dongjin-1, as cultivated rice was used at Fluvio-marine deposit in Honam plain paddy field. Soil chemical properties were improved by the application of MEC. Contents of total nitrogen and organic matter were higher in 70%, 100% plots of basal dressing than standard fertilizer application (SFA) plot. Cation exchangeable capacity was highly increased in 70% plot of basal dressing. Also, the content of organic matter in soil was increased with MEC application. Cation exchangeable capacity, total nitrogen and available phosphate were decreased according to late application time. The content of inorganic nitrogen in soil showed high tendency at more application rate of MEC, and nitrogen mineralization at harvest season have finished in 50%, 70% plots of basal dressing. The content of inorganic nitrogen in soil was increased according to late application time, however it was decreased in the late period of growth. Leaf color value became darker with increased application rate of MEC. Leaf color was dark green in MEC application plots at panicle formation stage, on the other hand, it was light green in 50%, 70% plots of basal dressing at heading stage. SPAD reading value of leaf-color was high during the whole growth stage in MEC application plots. More application rate of MEC showed higher tendency of fertilizer nitrogen absorption. Nitrogen use efficiency was the highest in 70% plot of basal dressing. Absorbed amount of fertilized nitrogen was increased in 10~15days before transplanting and nitrogen use efficiency was high according to the late application time. The ratio of perfect kernel and the content of protein on hulled rice showed high tendency at the less application rate of MEC. The ratio of head rice on milled rice showed high tendency at the less application rate of MEC. Rice yield increased 4% in 100% and 70% plots of basal dressing compare with SFA ($5.18Mg\;ha^{-1}$) plot respectively. Ear and culm length of rice were long according to the late application time, while the numbers of spikelet and ear were increased and the percentage of ripened grain was decreased. Rice yield was increased 2~5% in all MEC application plots compared to SFA plot and especially, increased 10~15days before transplanting in application plots. The optimal application rate and time of MEC on normal paddy field in plain were concluded that 70% basal dressing and 10~15days before transplanting

• Radiation Oncology Journal
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• v.22 no.4
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• pp.288-297
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• 2004

Puporse: The aims of this study were to evaluate the change of $[^18F]fluoromisonidazole$($[^18F]FMISO$) uptake in C3H mouse squamous cell carcinoma-VII (SCC-VII) treated with mild hyperthermia ($42^{circ}C$) and nicotinamide and to assess the biodistribution of the markers in normal tissues under similar conditions. Methods and Materials: $[^18F]FMISO$ was producedby our hospital. Female C3H mice with a C3H SCC-VII tumor grown on their extremities were used. Tumors were size matched. Non-anaesthetized, tumor-bearing mice underwent control or mild hyperthermia at $42^{circ}C$ for 60 min with nicotinamide (50 mg/kg i.p. injected) and were examined by gamma counter, autoradiography and animal PET scan 3 hours after tracer i.v. injected with breathing room air, The biodistribution of these agents were obtained at 3 h after $[^18F]FMISO$ injection. Blood, tumor, muscle, heart, lung, liver, kidney, brain, bone, spleen, and intestine were removed, counted for radioactivity and weighed. The tumor and liver were frozen and cut with a cryomicrotome into 10- um sections. The spatial distribution of radioactivity from the tissue sections was determined with digital autoradiography. Results: The mild hyperthermia with nicotinamide treatment had only slight effects on the biodistribution of either marker in normal tissues. We observed that the whole tumor radioactivity uptake ratios were higher in the control mice than in the mild hyperthermia with nicotinamide treated mice for $[^18F]FMISO$ ($1.56{\pm}1.03$ vs. $0.67{\pm}0.30$; p=0.063). In addition, autoradiography and animal PET scan demonstrated that the area and intensity of $[^18F]FMISO$ uptake was significantly decreased. Conclusion: Mild hyperthermla and nicotinamide significantly improved tumor hypoxia using $[^18F]FMISO$ and this uptake reflected tumor hypoxic status.

• Journal of Veterinary Clinics
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• v.10 no.1
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• pp.111-130
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• 1993

Lead toxicity was evaluated in forty-five cats on a balanced diet, Treated with 0(control), 10, 100(low), 1, 000, 2, 000, and 4, 000(high) ppm of lead acetate orally on a body weight basis. The objectives were to establish toxic dosage level of leaf in cats, to characterize changes in behavior and clinical pathology, and to demonstrate what blood lead concentrations correlate with the known dosages of lead. Some high dose cats showed projectile vomiting, hyperactivity, and seizures. The growth rates did not appear to be altered in any of the dosed groups. Normal blood lead concentration in cats were lower than that of humans, dogs, and cattle. Blood lead concentrations of 3 to 20$\mu\textrm{g}$/100$m\ell$ could be termed a 'subclinical' range in the cat. Clinical lead toxicity in cats may have blood lead concentrations ranging 20 to 120$\mu\textrm{g}$/100$m\ell$. Zinc protoporphyrin concentrations were proportional to lead dosages and a significant ZPP elevation, greater than 50$\mu\textrm{g}$/100$m\ell$, may be indicative of clinical lead toxicity. The enzyme aminolevulinic acid dehydratase showed an inverss dose response relationship for all lead dosages and a significant ZPP elevation, greater than 50$\mu\textrm{g}$/100$m\ell$, may be indicative of clinical lead toxicity. The enzyme aminolevulinic acid dehydratase showed an inverse dose response relationship for all lead dosages and appears to be a good indicator of lead exposure in cats. Urinary aminolevuliruc acid concentrations generally increased with lead dosage, but individual values varied. Hair lead concentrations rose proportionately to lead dosages. Lead at least in high doses appears to inhibit chemotactic activity of polymorphonuclear cells and monocytes. No consistent dose response relationships were observed in hemoglobin, RBC, WBC, neutrophil, lymphocyte, monocyte, and eosinophil counts. There were no consistent dose related changes in total protein, plasma protein, BUN, and ALT values. Reticulocyte counts did not increase significantly in most lead dosage levels, and are probably of little value in diagnosing lead toxicity in cats. The fact that no significant changes were found in nerve conduction velocities may support that there was no segmental demyelination resulting from lead ingestion. The lethal dose in cats appear to range from 60 to 150mg/kg body weight. A reliable diagnosis of lead poisoning can be made utilizing blood lead, ZPP, and ALAD, and hair lead.