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STABILIZATION AND RECLAMATION OF OLD LANDFILL DISPOSAL SITES

  • Kemper P.E., Charles C.
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 1996.12a
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    • pp.87-95
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    • 1996
  • The stabilization and reclamation of old disposal sites is becoming more important as significant numbers of disposal sites are closed and abandoned. This technical paper covers an overview of the key issues and methodologies for stabilizing and constructing facilities on old landfills. The slide portion of this presentation also include photographs showing actual construction activities. The key issues that are prevalent in remediating and closing old landfills are : correcting the stormwater flow, leachate breakout, constructing cover caps, controlling landfill gas migration and odors, cleanup groundwater and stabilizing side slopes. Some key techniques for constructing facilities on old landfills include: use of piling, installation of active landfill gas systems, providing LFG barriers under buildings, using utilidors and flexible utility interfaces and designing for site settlement. This Paper provides proven conceptual methods for solving these problems.

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Suggestion of Safety Management Activities and Key Check point for Safety Management in Modular Construction Sites (모듈러 건설현장의 단위작업별 안전관리 활동 및 중점 체크사항 제안)

  • Jun, Young-Hun;Kim, Kyoon-Tai
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2022.04a
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    • pp.202-203
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    • 2022
  • In this study, focusing on work types of lifting modules and modules assembly in modular construction sites, unit works were defined according to the construction process of each work type, and safety management elements, safety management activities, and key check point were proposed. The results of this study may have somewhat overlapping contents in the construction procedure in order to derive specific safety management activities and check point for each unit work. Therefore, there is a limit to use the results in an actual modular construction site. In future research, we plan to improve this part and derive a checklist that considers field usability.

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Improvement of Construction Management Process for the Site Manager in Small Scale Building Construction Project (소규모 건설프로젝트에서 현장관리업무 개선방안)

  • Lee, Da-Un;Yun, Seok-Heon
    • Journal of the Korea Institute of Building Construction
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    • v.18 no.1
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    • pp.81-87
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    • 2018
  • Since February 2017, site managers in small architectural projects are required by the revised building act. In order to address issues for site managers and to improve project efficiency, system improving methods are surveyed and proposed in this study. The key inspection items in the existing surveillance guidance manual were investigated, and the checklist for site managers for a small to medium sized construction site was analyzed. This study also tries to propose using web-based site management systems for improvement of the utilization of checklists. It is expected that these on-site management systems, along with BIM technology, will be able to monitor the small size building construction project in real time and effectively monitor the various problems occurring on-site.

RPK118, a PX Domain-containing Protein, Interacts with Peroxiredoxin-3 through Pseudo-Kinase Domains

  • Liu, Lungling;Yang, Chenyi;Yuan, Jian;Chen, Xiujuan;Xu, Jianing;Wei, Youheng;Yang, Jingchun;Lin, Gang;Yu, Long
    • Molecules and Cells
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    • v.19 no.1
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    • pp.39-45
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    • 2005
  • RPK118 is a sphingosine kinase-1-binding protein that has been implicated in sphingosine 1 phosphate-mediated signaling. It contains a PX (phox homology) domain and two pseudo-kinase domains, and co-localizes with sphingosine kinase-1 on early endosomes. In this study we identified a novel RPK118-binding protein, PRDX3 (peroxiredoxin-3), by yeast two-hybrid screening. The interaction between these proteins was confirmed by pull-down assays and co-immunoprecipitation experiments. Deletion studies showed that RPK118 interacted with PRDX3 through its pseudokinase domains, and with early endosomes through its PX domain. Double immunofluorescence experiments demonstrated that PRDX3 co-localized with RPK118 on early endosomes in COS7 cells. PRDX3 is a member of the antioxidant family of proteins synthesized in the cytoplasm and functioning in mitochondria. Our findings indicate that RPK118 is a PRDX3-binding protein that may be involved in transporting PRDX3 from the cytoplasm to its mitochondrial site of function or to other membrane structures via endosome trafficking.

Asn124 of Cel5A from Hypocrea jecorina not only provides the N-glycosylation site but is also essential in maintaining enzymatic activity

  • Qin, Yuqi;Qu, Yinbo
    • BMB Reports
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    • v.47 no.5
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    • pp.256-261
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    • 2014
  • To investigate the function of N-glycosylation of Cel5A (endoglucanase II) from Hypocrea jecorina, two N-glycosylation site deletion Cel5A mutants (rN124D and rN124H) were expressed in Saccharomyces cerevisiae. The weights of these recombinant mutants were 54 kDa, which were lower than that of rCel5A. This result was expected to be attributed to deglycosylation. The enzyme activity of rN124H was greatly reduced to 60.6% compared with rCel5A, whereas rN124D showed slightly lower activity (10%) than that of rCel5A. rN124D and rN124H showed different thermal stabilities compared with the glycosylated rCel5A, especially at lower pH value. Thermal stabilities were reduced and improved for rN124D and rN124H, respectively. Circular dichroism spectroscopy showed that the modification of secondary structure by mutation may be the reason for the change in enzymatic activity and thermal stability.

A Study on the Architectural Thought and Its Construction shown in F. L. Wright's Taliesin West' (프랭크 로이드 라이트의 '텔리에센 웨스트'의 건축화 과정에 관한 연구)

  • Park, Jong-Sung
    • Korean Institute of Interior Design Journal
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    • v.16 no.3 s.62
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    • pp.3-9
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    • 2007
  • Taliesin West is a meaningful historic site in architecture field, because its place had still been existing only one in the world as a ideal architectural community for working and living as well as learning by doing for Taliesin Fellowships and others based on F. L. Wright's idea of Organic Architecture. The main purpose of this study was to follow up the architectural thought and its construction shown in F. L. Wright's 'Taliesin West'. A study on the key notes are as follows; 1) The key-clue of the construction background for Taliesin West was based on the project of 'Complex Campus Building' which was early planed by F. L. Wright. 2) A basic design idea for Taliesin West was admiring from its own site characters as well as the Experimental construction methods and materials. 3) Design motive of Taliesin West was based on American Indian's movable shelter which called 'Tepee.' 4) A construction of F. L. Wright's temporary studio, Ocotilla, was a good opportunity to construct for Taliesin West which construction methods, covered and framed, were same as Ocotilla. 5) A concept idea of the master plan for Taliesin West came from combining Taliesin's Hillside Home School and Complex Campus Building project. 6) Construction of Taliesin West was a final accomplished place as F. L. Wright's utopia architecture and community.

Interpretation of Varietal Response to Rice Leaf Blast by G$\times$E Analysis with Reduced Number of Nursery Test Sites

  • Yang, Chang-Ihn;E. L. Javier;Won, Yong-Jae;Yang, Sae-Jun;Park, Hae-Chune;Shin, Young-Boum
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.5
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    • pp.316-321
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    • 2000
  • Blast severity data of 39 rice varieties at 11 sites in Korea from 1997 to 1999 were analyzed using AMMI model and pattern analysis. Genotype x Environment (G$\times$E) interaction sum of squares (SS) accounted for 12 % of the total SS. Eight genotype groups and seven location groups were identified based on blast reaction pattern. The data obtained from over 21 sites with 44 test varieties from 1981 to 1996 were also considered. These were compared with the 1997-1999 data using the G$\times$E analysis results. Majority of the variability in the Korean Rice Blast Nursery (KRBN) were attributable to variations due to genotypes. Variations of G$\times$E interaction were maintained though test sites were reduced from 21 to 11 sites. Broadly compatible biological discriminative varieties identified were Nagdongbyeo and Akibare while broadly incompatible biological discriminative varieties identified were Hangangchalbyeo and Seogwangbyeo. Key sites for future evaluation work could be selected from location groups. Each location group should be represented by the site with the strongest interaction pattern. Blast responses in Cheolwon, Gyehwa, Suwon, Iksan, and Icheon showed different patterns from other locations.

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PROMISE: A QR Code PROjection Matrix Based Framework for Information Hiding Using Image SEgmentation

  • Yixiang Fang;Kai Tu;Kai Wu;Yi Peng;Yunqing Shi
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.17 no.2
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    • pp.471-485
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    • 2023
  • As data sharing increases explosively, such information encoded in QR code is completely public as private messages are not securely protected. This paper proposes a new 'PROMISE' framework for hiding information based on the QR code projection matrix by using image segmentation without modifying the essential QR code characteristics. Projection matrix mapping, matrix scrambling, fusion image segmentation and steganography with SEL(secret embedding logic) are part of the PROMISE framework. The QR code could be mapped to determine the segmentation site of the fusion image as a binary information matrix. To further protect the site information, matrix scrambling could be adopted after the mapping phase. Image segmentation is then performed on the fusion image and the SEL module is applied to embed the secret message into the fusion image. Matrix transformation and SEL parameters should be uploaded to the server as the secret key for authorized users to decode the private message. And it was possible to further obtain the private message hidden by the framework we proposed. Experimental findings show that when compared to some traditional information hiding methods, better anti-detection performance, greater secret key space and lower complexity could be obtained in our work.

Near-ground boundary layer wind characteristics analysis of Typhoon "Bailu" based on field measurements

  • Dandan Xia;Li Lin;Liming Dai;Xiaobo Lin
    • Wind and Structures
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    • v.39 no.1
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    • pp.15-30
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    • 2024
  • In this paper, detailed wind field data of the full path of typhoon "Bailu" were obtained based on site measurements. Typhoon "Bailu" made first landfall southeast of the Taiwan Strait with a wind speed of approximately 30 m/s near the center of the typhoon eye and a second landfall in Dongshang County in Fujian Province. The moving process is classified into 3 regions for analysis and comparison. Detailed analyses of wind characteristics including wind profile, turbulence intensity, gust factor, turbulence integral scale and wind power spectral density function at the full process of the typhoon are conducted, and the findings are presented in this paper. Wind speed shows significant dependence on both the direction of the moving path and the distance between the typhoon center and measurement site. Wind characteristics significantly vary with the moving path of the typhoon center. The relationship between turbulence intensity and gust factor at different regions is investigated. The integral turbulence scales and wind speed are fitted by a Gaussian model. Such analysis and conclusions may provide guidance for future bridge wind-resistant design in engineering applications.

Correlation analyses of CpG island methylation of cluster of differentiation 4 protein with gene expression and T lymphocyte subpopulation traits

  • Zhao, Xueyan;Wang, Yanping;Guo, Jianfeng;Wang, Jiying
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.8
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    • pp.1141-1149
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    • 2018
  • Objective: Cluster of differentiation 4 protein (CD4) gene is an important immune related gene which plays a significant role in T cell development and host resistance during viral infection. Methods: In order to unravel the relationship of CpG island methylation level of CD4 gene with its gene expression and T lymphocyte subpopulation traits, we used one typical Chinese indigenous breed (Dapulian, DP) and one commercial breed (Landrace), then predicted the CpG island of CD4 gene, determined the methylation status of CpG sites by bisulfite sequencing polymerase chain reaction (BSP), and carried out the correlation analyses of methylation frequencies of CpG sites with mRNA expression and T lymphocyte subpopulation traits. Results: There was one CpG island predicted in the upstream -2 kb region and exon one of porcine CD4 gene, which located 333 bp upstream from the start site of gene and contained nine CpG sites. The correlation analysis results indicated that the methylation frequency of CpG_2 significantly correlated with CD4 mRNA expression in the DP and Landrace combined population, though it did not reach significance level in DP and Landrace separately. Additionally, 15 potential binding transcription factors (TFs) were predicted within the CpG island, and one of them (Jumonji) contained CpG_2 site, suggesting that it may influence the CD4 gene expression through the potential binding TFs. We also found methylation frequency of CpG_2 negatively correlated with T lymphocyte subpopulation traits CD4+CD8-CD3-, CD4-CD8+CD3- and CD4+/CD8+, and positively correlated with CD4-CD8+CD3+ and CD4+CD8+CD3+ (for all correlation, p<0.01) in DP and Landrace combined population. Thus, the CpG_2 was a critical methylation site for porcine CD4 gene expression and T lymphocyte subpopulation traits. Conclusion: We speculated that increased methylation frequency of CpG_2 may lead to the decreased expression of CD4, which may have some kind of influence on T lymphocyte subpopulation traits and the immunity of DP population.