• 한국정보시스템학회지:정보시스템연구
• /
• 제20권4호
• /
• pp.1-22
• /
• 2011

This study addressed trust to collective intelligence for explaining the affecting factors to the intention to use of collective intelligence by dividing the object of trust into a Web site and an information source group. We explored the factors affecting user's continuance intention toward collective intelligence in the view off trust building. We made a well-structured survey of our proposed model and gained 205 cases. We analyzed the proposed research model empirically using partial least square method. The findings are summarized as follows. First, all key factors (relevance, timeless, completeness, understandability) composing of collective intelligence quality have a positive and significant impact on confirmation. Second, confirmation has a significant impact on trust toward a Web site, as well as toward an information source group. The last is that trust toward a Web site influences on continuance intention, whereas trust toward an information source group doesn't.

• 한국콘크리트학회:학술대회논문집
• /
• 한국콘크리트학회 2004년도 추계 학술발표회 제16권2호
• /
• pp.461-464
• /
• 2004

External prestressing method is the most papular method to strengthen concrete structures. The key parts of external prestressing method is an anchoring device which holds the prestressing cables. The most popular anchoring devices is an end bearing type, which has to be welded at site to assamble the anchor. The welding at site has a significant Quality problem. In this paper, new anchoring system has been developed, which needs no site welding. A design method for the new anchor has been proposed and developed to optimize the anchoring system. The developed anchors are very efficient, small, and compact, which have two types, one for abutment section and the other one for pier section. The anchors are expected to be used very widely, because of its convinience.

• 한국건축시공학회:학술대회논문집
• /
• 한국건축시공학회 2019년도 추계 학술논문 발표대회
• /
• pp.44-45
• /
• 2019

Tower cranes are essential equipments that play a key role in the overall process of large-scale construction projects, as well as high-rise apartments construction projects. Tower cranes hoist various loads near and over people, working in crowded environments, sometimes in overlapping work areas, and often under the constraints of time, budget, and labor. This work system further increases the safety risks of sites with inherently hazardous workplaces. The methodology of this research was based on a comprehensive inquiry of a team of experts, including safety managers and equipment managers of major construction companies and previous literature search. Due to the limited resources available to improve safety and prevent accidents, more attention should be paid to all parties involved in factors that have been evaluated to have a significant impact on site safety due to tower crane operations.

• 한국생산제조학회지
• /
• 제19권5호
• /
• pp.667-671
• /
• 2010

The study was aimed at developing a power transmission coupling that is possible to transfer power without any trouble even if the two rotating shafts are making minute misalignment. The coupling that has been developed is Flange Flexible Urethane-key Coupling that connects two shafts by flanges with Urethane-key. A model coupling for use in transmitting power of 10hp was made and undergone performance evaluation and tests. Property and usefulness was proved through the test. The performance evaluation has demonstrated a property of $11.25Kgf{\cdot}m$ of allowable torque and 28.25hp of power at 1,80Orpm, which was found to be superior compared to the performance of similar couplings. Based on the performance test, study was made also for improving the shape of the Urethane-key and was successful to make the flange in smaller outside diameter. Further application test at site has proved that the product is easy to install and maintain, and has property of absorbing minute misalignment between two shafts and vibration caused there from.

• Geomechanics and Engineering
• /
• 제18권6호
• /
• pp.627-638
• /
• 2019

Estimation of representative elementary volume (REV) of jointed rock masses is critical to predict the mechanical behavior of field-scale rock masses. The REV of jointed rock masses at site is strongly influenced by stress state. The paper proposed a method to systematically studied the influence of confining stress on the REV of jointed rock masses with various strengths (weak, medium and strong), which were sourced from the water inlet slope of Xiaowan Hydropower Station, China. A finite element method considering material heterogeneity was employed, a series of two-dimensional (2D) models was established based on the Monte-Carlo method and a lot of biaxial compressive tests were conducted. Numerical results showed that the REV of jointed rock masses presented a step-like reduction as the normalized confining stress increased. Confining stress weakened the size effect of jointed rock masses, indicating that the REV determined under uniaxial compression test can be reasonably taken as the REV of jointed rock masses under complexed in-situ stress environment.

• Journal of Veterinary Science
• /
• 제21권2호
• /
• pp.19.1-19.10
• /
• 2020

Given that the current Newcastle disease virus (NDV) infection in wild birds poses the threat to poultry, surveillance of Newcastle disease in captive wild birds was carried out in Jilin, China in 2018. Here, an NDV strain obtained from toco toucan was firstly characterized. The results showed that the F gene of the NDV isolate Toucan/China/3/2018 is classified as genotype II in class II. Sequence analysis of the F0 cleavage site was ¹¹³RQGR/L¹¹⁷, which supports the result of the intracerebral pathogenicity index assay indicating classification of the isolate as low-pathogenicity. Experimental infection demonstrated that Toucan/China/3/2018 can effectively replicate and transmit among chickens. To our knowledge, this is the first report on genetically and pathogenically characterizing NDV strain isolated from toucan, which enriches the epidemiological information of NDV in wild birds.

• BMB Reports
• /
• 제51권11호
• /
• pp.572-577
• /
• 2018

Recent studies showed that the PD-1/PD-L1 checkpoint blockade is a dramatic therapy for melanoma by enhancing antitumor immune activity. Currently, major strategies for the PD-1/PD-L1 blockade have mainly focused on the use of antibodies and compounds. Seeking an alternative approach, others employ endogenous proteins as blocking agents. The extracellular domain of PD-1 (ePD1) includes the binding site with PD-L1. Accordingly, we constructed a PD-1-based recombinantly tailored fusion protein (dFv-ePD1) that consists of bivalent variable fragments (dFv) of an MMP-2/9-targeted antibody and ePD1. The melanoma-binding intensity and antitumor activity were also investigated. We found the intense and selective binding capability of the protein dFv-ePD1 to human melanoma specimens was confirmed by a tissue microarray. In addition, dFv-ePD1 significantly suppressed the migration and invasion of mouse melanoma B16-F1 cells, and displayed cytotoxicity to cancer cells in vitro. Notably, dFv-ePD1 significantly inhibited the growth of mouse melanoma B16-F1 tumor cells in mice and in vivo fluorescence imaging showed that dFv-ePD was gradually accumulated into the B16-F1 tumor. Also the B16-F1 tumor fluorescence intensity at the tumor site was stronger than that of dFv. This study indicates that the recombinant protein dFv-ePD1 has an intensive melanoma-binding capability and exerts potent therapeutic efficacy against melanoma. The novel format of the PD-L1-blocked agent may play an active role in antitumor immunotherapy.

• Journal of Microbiology and Biotechnology
• /
• 제31권2호
• /
• pp.259-271
• /
• 2021

Many bacteria metabolize aromatic compounds via catechol as a catabolic intermediate, and possess multiple genes or clusters encoding catechol-cleavage enzymes. The presence of multiple isozyme-encoding genes is a widespread phenomenon that seems to give the carrying strains a selective advantage in the natural environment over those with only a single copy. In the naphthalene-degrading strain Pseudomonas putida ND6, catechol can be converted into intermediates of the tricarboxylic acid cycle via either the ortho- or meta-cleavage pathways. In this study, we demonstrated that the catechol ortho-cleavage pathway genes (catBICIAI and catBIICIIAII) on the chromosome play an important role. The catI and catII operons are co-transcribed, whereas catAI and catAII are under independent transcriptional regulation. We examined the binding of regulatory proteins to promoters. In the presence of cis-cis-muconate, a well-studied inducer of the cat gene cluster, CatRI and CatRII occupy an additional downstream site, designated as the activation binding site. Notably, CatRI binds to both the catI and catII promoters with high affinity, while CatRII binds weakly. This is likely caused by a T to G mutation in the G/T-N11-A motif. Specifically, we found that CatRI and CatRII regulate catBICIAI and catBIICIIAII in a cooperative manner, which provides new insights into naphthalene degradation.

• KSBB Journal
• /
• 제19권5호
• /
• pp.406-409
• /
• 2004

Matrix Assisted Laser Desorption ionization Time of Flight (MALDI-TOF) was used for enzymes identification related to B -1,3-glucan synthesis. Agrobacterium sp. ATCC31750 was cultivated with two stage Continuous Stirrer Tank Reactor (CSTR) and the cells were harvested and their protein profiles were analysed by two dimensional electrophoresis. The specific enzyme spot was treated with trypsin and ana lysed by MALDI-TOF to get peptide molecular weight. The peptide molecular weights were matched with Agrobacterium tumefacience's Data Base from the matrix science site, then could identify the avaliable key enzymes. In this study, we identified key metabolite of synthesis of beta-1,3-glucan, such as glucose-6-phosphate isomerase, phosphoglucomutase, B-1,3-glucan synthase and glucokinase, and we also identified uracil phosphoribocyl transferase and Ribosome recycling factor also.

• 국제학술발표논문집
• /
• The 9th International Conference on Construction Engineering and Project Management
• /
• pp.361-369
• /
• 2022

Unsafe actions and behaviors of workers cause most accidents at construction sites. Nowadays, occupational safety is a top priority at construction sites. However, this problem often requires money and effort from investors or construction owners. Therefore, decreasing the accidents rates of workers and saving monitoring costs for contractors is necessary at construction sites. This study proposes an unsafe behavior detection method based on a skeleton model to classify three common unsafe behaviors on the scaffold: climbing, jumping, and running. First, the OpenPose method is used to obtain the workers' key points. Second, all skeleton datasets are aggregated from the temporary size. Third, the key point dataset becomes the input of the action classification model. The method is effective, with an accuracy rate of 89.6% precision and 90.5% recall of unsafe actions correctly detected in the experiment.