• 제목/요약/키워드: Key site

검색결과 905건 처리시간 0.027초

New Gene Cluster from Thermophile Bacillus fordii MH602 for Conversion of DL-5-Substituted Hydantoins to L-Amino Acids

  • Mei, Yan-Zhen;Wan, Yong-Min;He, Bing-Fang;Ying, Han-Jie;Ouyang, Ping-Kai
    • Journal of Microbiology and Biotechnology
    • /
    • 제19권12호
    • /
    • pp.1497-1505
    • /
    • 2009
  • The thermophile Bacillus fordii MH602 was screened for stereospecifically hydrolyzing DL-5-substituted hydantoins to L-$\alpha$-amino acids. Since the reaction occurs at higher temperature, the advantages for enhancement of substrate solubility and for racemization of DL-5-substituted hydantoins during the conversion were achieved. The hydantoin metabolism gene cluster from thermophile is firstly reported in this paper. The genes involved in hydantoin utilization (hyu) were isolated on an 8.2-kb DNA fragment by restriction site-dependent PCR, and six ORFs were identified by DNA sequence analysis. The hyu gene cluster contained four genes with novel cluster organization characteristics: the hydantoinase gene hyuH, putative transport protein gene hyuP, hyperprotein gene hyuHP, and L-carbamoylase gene hyuC. The hyuH and hyuC genes were heterogeneously expressed in E. coli. The results indicated that hyuH and hyuC are involved in the conversion of DL-5-substituted hydantoins to an N-carbamyl intermediate that is subsequently converted to L-$\alpha$-amino acids. Hydantoinase and carbamoylase from B. fordii MH602 compared respectively with reported hydantoinase and carbamoylase showed the highest identities of 71% and 39%. The novel cluster organization characteristics and the difference of the key enzymes between thermopile B. fordii MH602 and other mesophiles were presumed to be related to the evolutionary origins of concerned metabolism.

소셜미디어 빅데이터의 텍스트 마이닝과 오피니언 마이닝 기법을 활용한 웹드라마 분석과 제안 (Webdrama Analysis and Recommendation using Text Mining and Opinion Mining Technique of Social Media)

  • 오세종;김치호
    • 만화애니메이션 연구
    • /
    • 통권44호
    • /
    • pp.285-306
    • /
    • 2016
  • 1인 스마트폰 사용으로 웹툰, 웹소설, TV드라마는 생산자에서 소비자에게 직접적으로 소비할 수 있는 Direct-to-Consumer로 전환되고 있다. 특히, 포털사이트의 웹드라마는 새로운 미디어로 급성장하고 있다. '연애세포', '0시의 그녀', '최고의 미래', '우리 옆집에 EXO가 산다' 등을 TV드라마의 시청률처럼 조회수, 유입자, 댓글, 좋아요 등으로 다양한 반응을 분석할 수 있다. 분석 방법은 소셜미디어 빅데이터의 텍스트 마이닝 기법과 오피니언 마이닝 기법으로 작품을 분석했다. 즉, 웹드라마 마다의 특정 키워드를 추출하고, 추출한 키워드의 긍정, 부정, 중립 등 시청자의 감정을 예측할 수도 있다. 주요 인기 웹드라마를 분석한 결과로는 이미 팬을 확보한 K-Pop 아이돌 멤버의 출현과 포털사이트의 편성 회사와의 연관성이 재생수, 유입자, 댓글, 좋아요에 큰 영향을 미치는 것으로 나타났다. 또한 TV 이외의 매체로 '모바일 TV'의 영향력을 증명하였다. 한계점으로는 모바일 특화 콘텐츠 확보와 비즈니스 모델을 정립하는 것이 필요하겠다. 이 부분을 해결한다면, 한국은 웹드라마의 콘텐츠 강국이라는 긍정적 이미지를 보여줄 수 있는 계기가 될 것이다.

도시개발사업의 투자가치 평가를 통한 전략적 도시관리 방안에 관한 연구 - 용인시 구갈 역세권 지역을 중심으로 - (A Study on Strategic Direction of Urban Management through Evaluation of Value-for-Money for Urban Development Projects - Focused on the Region of Gugal Station Area in Yongin City -)

  • 황의표;원제무
    • 한국방재학회 논문집
    • /
    • 제10권3호
    • /
    • pp.1-11
    • /
    • 2010
  • 본 연구에서는 경기도 용인시 기흥구 구갈동(구갈역세권)과 보라구역, 중동구역 일대를 중심으로 개발계획, 개발잠재력, 입지여건과 아울러 녹색 및 재해예방 인프라에 대한 4가지 측면을 고려하여 대상지의 투자가치 평가를 통한 전략적 도시관리 방안을 연구하였다. 방법론 측면에서는 평가항목간의 상관관계를 고려할수 있는 네트워크 분석적 의사결정법을 통하여 판단해보고자 하였다. 분석 결과 개발계획에서는 개발용도, 키테넌트, 자금조달, 마케팅순으로, 개발잠재력에서는 시장성, 정체성, 역사성 순으로, 입지여건에서는 토지이용, 주변환경, 교통환경순으로, 마지막으로 녹색 및 재해예방인프라에서는 자연재해예방, 인적재해예방, 사회적 재해예방순으로 나타났다. 본 연구의 결과는 향후 도시개발사업 추진하는데 있어 합리적인 사전 평가방안으로 활용할 수 있을 것이라 본다.

THE POTENTIAL OF SATELLITE REMOTE SENSING ON REDUCTION OF TSUNAMI DISASTER

  • Siripong, Absornsuda
    • 대한원격탐사학회:학술대회논문집
    • /
    • 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume I
    • /
    • pp.52-55
    • /
    • 2006
  • It's used to be said that tsunami is a rare event. The recurrence time of tsunami in Sumatra area is approximately 230 years as CalTech Research Group‘s study from paleocoral. However, the tsunami occurred in Indian Ocean on 26 December 2004, 28 March 2005 and 17 July 2006, because the earthquakes still release the energy. To cope with the tsunami disaster, we have to put the much effort on better disaster preparedness. The Tsunami Reduction Of Impacts through three Key Actions (TROIKA) was suggested by Eddie N. Bernard, the director of NOAA/PMEL (Pacific Marine Environmental Laboratory). They are Hazard Assessment, Mitigation and Warning Guidance. The satellite remote sensing has potential on these actions. The medium and high resolution satellite data were used to assess the degree of damage at the six-damaged provinces on the Andaman seacoast of Thailand. Fast and reliable interpretation of the damage by remote sensing method can be used for inundation mapping, rehabilitation and housing plans for the victims. For tsunami mitigation, the satellite data can be used with GIS to construct the evacuation map (evacuation route and refuge site) and coastal zone management. It is also helpful for educational program for local residents and school systems. Tsunami is a kind of ocean wave, therefore any satellite sensors such as SAR, Altimeter, MODIS, Landsat, SPOT, IKONOS can detect the tsunami wave in 2004. The satellite images have shown the characteristics of tsunami wave approaching the coast. For warning, satellite data has potential for early warning to detect the tsunami wave in deep ocean, if there are enough satellite constellation to monitor and detect the first tsunami wave like the pressure gauge, seismograph and tide gauge with the DART buoy can do. Moreover, the new methods should be developed to analyse the satellite data more faster for early warning procedure.

  • PDF

APPLICATION OF MERGED MICROWAVE GEOPHYSICAL OCEAN PRODUCTS TO CLIMATE RESEARCH AND NEAR-REAL-TIME ANALYSIS

  • Wentz, Frank J.;Kim, Seung-Bum;Smith, Deborah K.;Gentemann, Chelle
    • 대한원격탐사학회:학술대회논문집
    • /
    • 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume I
    • /
    • pp.150-152
    • /
    • 2006
  • The DISCOVER Project (${\underline{D}}istributed$ ${\underline{I}}nformation$ ${\underline{S}}ervices$ for ${\underline{C}}limate$ and ${\underline{O}}cean$ products and ${\underline{V}}isualizations$ for ${\underline{E}}arth$ ${\underline{R}}esearch$) is a NASA funded Earth Science REASoN project that strives to provide highly accurate, carefully calibrated, long-term climate data records and near-real-time ocean products suitable for the most demanding Earth research applications via easy-to-use display and data access tools. A key element of DISCOVER is the merging of data from the multiple sensors on multiple platforms into geophysical data sets consistent in both time and space. The project is a follow-on to the SSM/I Pathfinder and Passive Microwave ESIP projects which pioneered the simultaneous retrieval of sea surface temperature, surface wind speed, columnar water vapor, cloud liquid water content, and rain rate from SSM/I and TMI observations. The ocean products available through DISCOVER are derived from multi-sensor observations combined into daily products and a consistent multi-decadal climate time series. The DISCOVER team has a strong track record in identifying and removing unexpected sources of systematic error in radiometric measurements, including misspecification of SSM/I pointing geometry, the slightly emissive TMI antenna, and problems with the hot calibration source on AMSR-E. This in-depth experience with inter-calibration is absolutely essential for achieving our objective of merging multi-sensor observations into consistent data sets. Extreme care in satellite inter-calibration and commonality of geophysical algorithms is applied to all sensors. This presentation will introduce the DISCOVER products currently available from the web site, http://www.discover-earth.org and provide examples of the scientific application of both the diurnally corrected optimally interpolated global sea surface temperature product and the 4x-daily global microwave water vapor product.

  • PDF

도로노면 유출수의 비점오염원 배출 특성(1): 기본 수질 항목 (Characteristics of Non-point Pollutants from the Road Runoff (1): Water Quality)

  • 박상우;오재일;최영화;서정우
    • 상하수도학회지
    • /
    • 제21권2호
    • /
    • pp.225-233
    • /
    • 2007
  • Road runoff water is one of the non-point sources (NPSs) of pollution negatively influencing drinking water source. Numerous road runoff NPS waters have been studied for over the last decade. However, the sources of pollution can be conditional, seasonal, or accidental. Therefore, measurement of pollutant loadings in different site is necessary to estimate the effect of road runoff water. The objective of this study was to examine the quality of road runoff water from a city bridge in Seoul, Korea. This study was conducted for two years to assess annual discharge pollution loads. In this study, key water quality parameters including chemical oxygen demand ($COD_{Cr}$), biochemcial oxygen demand ($BOD_5$), total nitrogen (T-N), total phosphorus (T-P), and suspended solid (SS) were measured at 18 different events. The results showed that typically the pollutant concentrations are higher at the beginning of each event and decrease afterwards. The first 20% of the volume of the runoff from each event is transporting 46% ($COD_{Cr}$), 48% ($BOD_5$), 50% (T-N), 34% (T-P), 30% (SS), respectively. The event mean concentrations (EMCs) were $COD_{Cr}$ (199 mg/L), $BOD_5$ (41.2 mg/L), T-N (7.97 mg/L), T-P (0.42 mg/L) and SS (113 mg/L). Although the results were consistent with the previous study (Barbosa and Hvitved-Jacobsen, 1999), $COD_{Cr}$, $BOD_5$, T-N exhibit a stronger first flush effect compared to the other contaminants.

도로노면 유출수의 비점오염원 배출 특성(2): 중금속 및 병원성 미생물 (Characteristics of Non-point Pollutants from the Road Runoff (2): Heavy Metals and Pathogens)

  • 박상우;오재일;최영화;김종화;하재원
    • 상하수도학회지
    • /
    • 제21권2호
    • /
    • pp.235-242
    • /
    • 2007
  • Road runoff water includes various heavy metals (zinc, Zn; lead, Pb; copper, Cu; chrome, Cr; cadmium, Cd; etc.) and pathogens (E-coli and coliform). Since these pollutants are significantly harmful to human beings and have negative impact on water streams, numerous studies have been conducted to determine the characterization of these non-point pollutants from road runoff water. However, since these non-point pollutant concentrations vary depending on road traffic, road construction, and road maintenance, measurement of pollutant loadings in different site is necessary to estimate the effect of road runoff water on drinking water source. The objective of this study was to examine the quality of road runoff water from a city bridge in Seoul, Korea. This study was conducted for two years to assess annual discharge pollution loads. In this study, five key heavy metals (Zn, Pb, Cu, Cr, and Cd) and two pathogens (E-coli and coliform) were measured at 18 different events. The pollutant load mass transported was always higher than the corresponding runoff volume for Zn, Cu, and Cd, while Pb and Cr showed similar values between the load mass transported and the corresponding runoff volume. The event mean concentrations were Zn (0.908 mg/L), Pb (0.092 mg/L), Cu (0.141 mg/L), Cr (0.023 mg/L), and Cd (0.006 mg/L). Like Zn, Cu, and Cd, E-coli and coliform values (relatively high in Summer and Fall) are higher at the beginning of each event and decrease afterwards.

하악골 골절에 대한 수술 시 예방적 항생제 사용과 술후 항생제 투여의 효율성 (ANTIBIOTIC PROPHYLAXIS IN THE OPERATION OF THE CLOSED MANDIBULAR FRACTURES AND THE EFFICACY OF POSTOPERATIVE ANTIBIOTICS)

  • 강상훈;최영수;변인영;김문기
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
    • /
    • 제35권1호
    • /
    • pp.31-34
    • /
    • 2009
  • Purpose: This study compared the frequency of postoperative infections in patients for a closed mandibular fracture with that without the postoperative antibiotic prophylaxis. Patients and Methods: 48 patients without any specific medical history were divided into two groups depending on whether or not antibiotics had been applied after the surgery. The 24 patients in group 1 received only a second-generation cephalosporin ($Cefotetan^{(R)}$) intravenously from admission to immediate after the surgery. Likewise, 24 patients in group 2 received 1.0g of $Cefotetan^{(R)}$ twice daily longer than the third day after surgery. The mean (SD) duration of antibiotics administration after surgery was 6.9 (${\pm}3.56$). The patients were evaluated after surgery for any postoperative infections according to the criteria: purulent drainage from a wound, spontaneous wound dehiscence accompanied by swelling, pain, and fever around the wound. Results: Postoperative infections were encountered in 2 out of 24 patients in group 1, who received antibiotic medication until shortly after surgery, and in 3 out of the 24 patients in group 2, in whom the medication was continued even after the surgery. There was no sig nificant difference in the incidence of postoperative infections between the two groups. Conclusion: From this study, postoperative use of antibiotics seems to be unnecessary with view of the little significance of the factors that could affect the wound infection.

SNS 브랜드 페이지에서 소비자-브랜드 관계 형성에 미치는 영향 요인 분석 - 준사회적 상호작용 역할을 중심으로 - (Factors affecting the development of consumer-brand relationships - The role of parasocial interaction -)

  • 박지선;하세진
    • 복식문화연구
    • /
    • 제25권1호
    • /
    • pp.88-103
    • /
    • 2017
  • The rapid growth in the popularity of social media sites has meant that social media has become an important communication channel for brands. Brands create brand pages on social media to cultivate positive and strong relationships with consumers. This study seeks to enhance our understanding of how fashion brand pages in social media foster consumer-brand relationships by exploring the factors that affect the development of consumer-brand relationships. Drawing on the parasocial interaction theory, this study proposes parasocial interaction as a key factor of this process. Specifically, this study proposes four antecedents (vividness, interactivity, social presence, and focused attention) of consumers' parasocial interaction with brand pages, which further affects consumer responses in terms of affective engagement and brand loyalty. An online survey was administered with consumers who have followed and visited at least one fashion brand page via a social networking site (SNS). Structural equation modeling revealed that consumers' perceptions of vividness, interactivity, social presence, and focused attention on a fashion brand page positively affected their feelings towards parasocial interaction with the brand page, which in turn led to their affective engagement with the brand page and consequent brand loyalty. These findings suggest that consumers build relationships of varying degrees with brand pages in a similar manner to that with people, which leads to their development of a positive relationship with the brand. This study concludes with discussions and practical implications.

한국의 경사지 밭의 토양 및 물의 보전 관리 전략 (Management Strategies to Conserve Soil and Water Qualities in the Sloping Uplands in Korea)

  • 양재이;유진희;김시주;정덕영
    • 농업과학연구
    • /
    • 제37권3호
    • /
    • pp.435-449
    • /
    • 2010
  • Soils in the sloping uplands in Korea are subject to intensive land use with high input of agrochemicals and are vulnerable to soil erosion. Development of the environmentally sound land management strategy is essential for a sustainable production system in the sloping upland. This report addresses the status of upland agriculture and the best management practices for the uplands toward the sustainable agriculture. More than 60% of Korean lands are forest and only 21% are cultivating paddy and upland. Uplands are about 7% of the total lands and about 62% of the uplands are in the slopes higher than 7%. Due to the site-specificity of the upland, many managerial and environmental problems are occurring, such as severe erosion, shallow surface soils with rocky fragments, and loadings of non-point source (NPS) contaminants into the watershed. Based on the field trials, most of the sloping uplands were classified as Suitability Class III-V and the major limiting factor was slope and rock fragments. Due to this, soils were over-applied with N fertilizer, even though N rate was the recommendation. This resulted in decreases in yield, degradation of soil quality and increases in N loading to the leachate. Various case studies drew management practices toward sustainable production systems. The suggested BMP on the managerial, vegetative, and structural options were to practice buffer strips along the edges of fields and streams, winter cover crop, contour and mulching farming, detention weir, diversion drains, grassed waterway, and slope arrangement. With these options, conservation effects such as reductions in raindrop impact, flow velocity, runoff and sediment loss, and rill and gully erosion were observed. The proper management practice is a key element of the conservation of the soil and water in the sloping upland.