• 대한안전경영과학회지
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• 제24권4호
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• pp.71-83
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• 2022

This study is to investigate the degree of field awareness of how each provision of the Serious Disaster Punishment Act will affect accident prevention. As a result of conducting a survey of construction site workers, it was analyzed that construction site workers had a low sense of safety and did not voluntarily engage in safety activities. And it can be seen that they are taking a very passive position that it is efficient to implement safety under the supervision of the supervisor. Workers who need voluntary construction site safety activities are most aware of the situation at the site, but the problem was pointed out as "lack of procedures for listening to opinions and preparing improvement measures" for these problems. Future research is needed to see if this is a problem for individual workers or for those in charge of safety and health who do not correct it even though active opinions have been submitted.

• 한국콘텐츠학회논문지
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• 제2권1호
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• pp.16-23
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• 2002

본 연구에서는 중소기업인들을 위한 디자인 교육 사이트의 부분으로서 I-business readiness 라는 이름으로 중소기업인들의 기존 사업을 Online을 통하여 확장하는 방법을 교육하는 컨텐츠를 개발하였으며 비즈니스 기획에 있어서의 디자인의 중요성을 강조하고 중소기업인들의 디자인에 대한 이해를 높이며 디자인을 사업에 잘 활용할 수 있는 방안을 제시하였다.

• 한국어교육
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• 제29권2호
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• pp.25-48
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• 2018

The aim of this study was to analyze research trends in the fields of Korean Language and Literature, Korean Language Education, and Korean Language Education as a Foreign Language. To this end, key words were extracted from 29,470 academic theses published between 2000 and 2017. The results of the analysis are as follows. First, in the field of Korean Language and Literature, there is greater quantity in studies about Korean language than about literature, and starting from 2010, there was an increase in studies similar to those from the field of Korean Language Education as a Foreign Language. Next, in comparison to the other fields, the field of Korean Language Education has greater variance in its research theme-in particular, numerous studies related to the site of education. Finally, the field of Korean Language Education has the following trends: a) there are copious studies focused on Korean language learners in comparison to other fields, b) there are a greater number of studies focused on culture, and c) the key words change by time period which suggest that research demands transformed over time. Overall, a total of 64 highest frequency key words from the three academic fields were investigated. Of these, 22 were common key words and 42 were differential key words. In this way, it was possible to illuminate the identity of each field.

• 해양환경안전학회지
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• 제19권3호
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• pp.233-240
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• 2013

Jakarta is the capital city of Indonesia which has problems of land subsidence with the rates of about 1 to 15 cm/year, up to 20-25 cm/year. The study has examined the land subsidence in Pantai Mutiara, Jakarta Bay which is a reclaimed area by using the Terrestrial LIDAR survey technique. The Terrestrial LIDAR survey results show that the survey site has mean elevation of 0.24 m with the highest elevation of 0.93 m and lowest - 0.35 m. Considering that AHHW (approximate highest high water) is 0.51 m, many areas of the survey site are lying below the AHHW. Pantai Mutiara area is showing various subsidence rates depending on sites although the site is relatively narrow and small (about 1 $km^2$). There is elevation differences of almost 1m within the site. In this study, key information including topography, dike height distribution, and future coastal flooding risk of the survey area was able to be provided by Terrestrial LIDAR survey conducted only once. Especially, as the 3D precision topography effectively conveys important messages relating to vulnerability of the site, policy makers and stakeholders can easily understand the situation of the site.

• 가족자원경영과 정책
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• 제11권2호
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• pp.133-154
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• 2007

This research investigated consumer's usage of, evaluation on and satisfaction of the Auction Site, with a view to analyze the key factors influencing a commission paid Auction Site. The findings of results from the analysis are summarized as follows. First of all, the consumer group at the age equal to and less than 20 years old showed the highest purchasing frequency, and the consumer group with a household income less than 2,500,000 won showed the highest number of hours on the site. Secondly, the evaluation index showed that consumers think highly of the complaint resolution process, and, especially, the aged consumer group respected the Auction Site's level of confidentiality of transaction and privacy. Thirdly, among consumers' satisfaction, subdivided by types, price satisfaction was the highest followed by quality satisfaction, settlement satisfaction, delivery satisfaction, information satisfaction and AS satisfaction, in that order. Fourthly, in the event consumers used the commission paid Auction Site, the number of times it has been used, evaluations related to information provision and evaluations on confidentiality of transactions and privacy are influential parameters.

• The Korean Journal of Physiology and Pharmacology
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• 제2권3호
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• pp.385-393
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• 1998

Human neutrophil elastase (HNElastase, EC 3.4.21.37), a causative factor of inflammatory diseases, was purified by Ultrogel AcA54 gel filtration and CM-Sephadex ion exchange chromatography. HNElastase was inhibited by phenylbutazone in a concentration dependent manner up to 0.4 mM, but as the concentration increased, the inhibitory effect gradually diminished. Binding of phenylbutazone to the human neutrophil elastase caused strong Raman shifts at 200, 440, and 1194 $cm^{-1}$. The peak at 1194 $cm^{-1}$ might be evidence of the presence $of\;-N=N-{\Phi}$ radical. The core area of the elastase, according to the visual molecular model of human neutrophil elastase, was structurally stable. A deeply situated active center was at the core area surrounded by hydrophobic amino acids. Directly neighboring the active site was one positively charged atom and two atoms carrying a negative charge, which enabled the enzyme and the drug to form a strong interaction. Phenylbutazone may form a binding, similar to a key & lock system to the atoms carrying opposite charges near the active site of the enzyme molecule. Furthermore, the hydrophobicity of the surrounding amino acid near the active site seemed to enhance the binding strength of phenylbutazone. Binding of phenylbutazone near the active site may cause masking of the active site, preventing the substrate from approaching the active site and inhibiting elastase activity.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.4031-4036
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• 2012

Background: The negative signaling provided by interactions of the co-inhibitory molecule, programmed death-1 (PD-1), and its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2), is a critical mechanism contributing to tumor evasion; blockade of this pathway has been proven to enhance cytotoxic activity and mediate antitumor therapy. Here we evaluated the anti-tumor efficacy of AAV-mediated delivery of the extracellular domain of murine PD-1 (sPD-1) to a tumor site. Material and Methods: An rAAV vector was constructed in which the expression of sPD-1, a known negative regulator of TCR signals, is driven by human cytomegalovirus immediate early promoter (CMV-P), using a triple plasmid transfection system. Tumor-bearing mice were then treated with the AAV/sPD1 construct and expression of sPD-1 in tumor tissues was determined by semi quantitative RT-PCR, and tumor weights and cytotoxic activity of splenocytes were measured. Results: Analysis of tumor homogenates revealed sPD-1 mRNA to be significantly overexpressed in rAAV/sPD-1 treated mice as compared with control levels. Its use for local gene therapy at the inoculation site of H22 hepatoma cells could inhibit tumor growth, also enhancing lysis of tumor cells by lymphocytes stimulated specifically with an antigen. In addition, PD-1 was also found expressed on the surfaces of activated CD8+ T cells. Conclusion: This study confirmed that expression of the soluble extracellular domain of PD-1 molecule could reduce tumor microenvironment inhibitory effects on T cells and enhance cytotoxicity. This suggests that it might be a potential target for development of therapies to augment T-cell responses in patients with malignancies.

• 컴퓨터교육학회논문지
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• 제7권5호
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• pp.83-91
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• 2004

본 논문에서 청소년들의 정신 건강을 해치는 유해 정보 사이트를 차단하기 위해 기존 방식이 아닌 내용 기반을 중심으로 하여 중요도가 가장 높은 한 개의 복합 키워드와 정보통신윤리 위원회에서 제시한 유해단어의 가중치를 이용하여 가중치 평균을 더해 유해도를 판단하여 유해 사이트와 일반 사이트를 구별하는 시스템을 구현하였다. 예비 실험을 통해 구해진 유해도의 값 3.5를 유해정보 사이트를 판단하는 기준으로 정한 다음 유해 정보 차단 시스템의 성능 실험을 위해 유해 정보 사이트와 일반 사이트를 각각 무작위로 100개씩 추출해 접속해 본 결과 유해 사이트를 유해 정보 사이트로 판명한 비율이 78%를 보였고 일반 사이트를 일반 사이트로 판명한 비율이 96%가 되어 본 시스템의 유효성을 확인 할 수가 있었다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제27권spc호
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• pp.64-74
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• 2022

The conceptual site model (CSM) is used as a key tool to support decision making in risk based management of contaminated sites. In this work, CSM was applied in Jeonju Industrial Complex where site investigation for groundwater contamination was conducted. Site background information including facility types, physical conditions, contaminants spill history, receptor exposure, and ecological information were collected and cross-checked with tabulated checklist necessary for CSM application. The CSM for contaminants migration utilized DNAPL transport model and narrative CSMs were constructed for source to receptor pathway, ecological exposure route, and contaminants fate and transport in the form of a diagram or flowchart. The component and uncertainty of preliminary CSM were reviewed using the data gap analysis while taking into account the purpose of the survey and the site management stage at the time of the survey. Through this approach, the potential utility of CSM was demonstrated in the site management process, such as assessing site conditions and planning follow-up survey work.

• Asian-Australasian Journal of Animal Sciences
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• 제28권10호
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• pp.1380-1387
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• 2015

This study aims to determine the polymorphism and mRNA expression pattern of the heart-type fatty acid-binding protein (H-FABP) gene and their association with intramuscular fat (IMF) content in the breast and leg muscles of Baicheng oil chicken (BOC). A total of 720 chickens, including 240 black Baicheng oil chicken (BBOC), 240 silky Baicheng oil chicken (SBOC), and 240 white Baicheng oil chicken (WBOC) were raised. Three genotypes of H-FABP gene second extron following AA, AB, and BB were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) strategy. The G939A site created AA genotype and G956A site created BB genotype. The content of IMF in AA genotype in breast muscle of BBOC was significantly higher than that of AB (p = 0.0176) and the genotype in leg muscle of WBOC was significantly higher than that of AB (p = 0.0145). The G939A site could be taken as genetic marker for higher IMF content selecting for breast muscle of BBOC and leg muscle of WBOC. The relative mRNA expression of H-FABP was measured by real-time PCR at 30, 60, 90, and 120 d. The IMF content significantly increased with age in both muscles. The mRNA expression level of H-FABP significantly decreased with age in both muscles of the three types of chickens. Moreover, a significant negative correlation between H-FABP abundance and IMF content in the leg muscles of WBOC (p = 0.035) was observed. The mRNA expression of H-FABP negatively correlated with the IMF content in both breast and leg muscles of BOC sat slaughter time.