• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1760-1772
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• 2006

Mature acetylcholinesterase (AChE) gene (gm, 1,836 bp) was cloned from the housefly and successfully expressed in the E. coli CodonPlus (DE3) RIL system (GM-E, 72 kDa) with a yield of 1,630 mU/g fresh cells. Using the gm, 10 kinds of mutants were constructed and expressed for enhancing sensitivity to insecticides. The sensitivity of these mutants to five kinds of organophosphate (OP) and three carbamate insecticides was investigated by measuring the apparent bimolecular inhibition constant ($k_i=k_2/K_d$). Surprisingly, the sensitivity of quadruple mutant IGFT was enhanced as much as 7-fold for acephate, 164-fold for demeton-S-methyl, 484-fold for dichlorvos, 523-fold for edifenphos, 30-fold for ethoprophos, 30-fold for benfuracarb, 404-fold for carbaryl, and 107-fold for furathiocarb, compared with that of GM-E, although the sensitivity of each single point mutant was slightly increased. These mutational studies indicated that (i) contradictory to Walsh et al. [39], the residue 327 is the important key residue for enhancing sensitivity as much as the residue 262, (ii) the residue 82 and additional residues of 234, 236, and 585 are also important, and (iii) sensitivity was cooperatively accelerated as the number of strategic mutations increased.

• Korean Journal of Agricultural and Forest Meteorology
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• v.7 no.1
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• pp.4-14
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• 2005

CarboKorea and HydroKorea are the domestic projects aiming to improve our understanding of carbon and water cycles in a typical Korean forest located in a complex terrain with a watershed connected to large rivers. The ultimate goal is to provide a nowcasting of these cycles for the whole Peninsula. The basic strategy to achieve such goal is through the inter- and multi-disciplinary studies that synthesize the in-situ field observation, modeling and remote sensing technology. The challenge is the fact that natural ecosystems are nonlinear and heterogeneous with a wide range of spatio-temporal scales causing the variations of mass and energy exchanges from a leaf to landscape scales. Our paradigm now shifts from temporal variation at a point to spatial patterns and from spatial homogeneity to complexity of water and carbon at multiple scales. Yet, a large portion of our knowledge about land-atmosphere interactions has been established based on tower observations, indicating that the development of scaling logics holds the key to the success of CarboKorea and HydroKorea. Here, we review the pioneering work of FIFE (First ISLSCP Field Experiment) on scaling issues in a temperate grassland and discuss the lessons from it for the application to Gwangneung forest site.

• Geomechanics and Engineering
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• v.15 no.2
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• pp.775-781
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• 2018

Shake table tests were conducted on scaled tunnel model to investigate the mechanism and effect of seismic loadings on horseshoe scaled tunnel model in ground fissure site. Key technical details of the experimental test were set up, including similarity relations, boundary conditions, sensor layout, modelling methods were presented. Synthetic waves and El Centro waves were adopted as the input earthquake waves. Results measured from hanging wall and foot wall were compared and analyzed. It is found that the seismic loadings increased the subsidence of hanging wall and lead to the appearance and propagation of cracks. The values of acceleration, earth pressure and strain were greater in the hanging wall than those in the foot wall. The tunnel exhibited the greatest earth pressure on right and left arches, however, the earth pressure on the crown of arch is the second largest and the inverted arch has the least earth pressure in the same tunnel section. Therefore, the effect of the hanging wall on the seismic performance of metro tunnel in earth fissure ground should be considered in the seismic design.

• International Journal of Advanced Culture Technology
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• v.4 no.2
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• pp.7-18
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• 2016

The first D2C-era web-real variety show in Korea was broadcast via tvN of CJ E&M. The web-real variety program 'SinSeoYuGi' accumulated 54 million views, along with 50 million views at the Chinese portal site QQ. This study carries out an analysis using text mining that extracts portal site blogs, twitter page views and associative terms. In addition, this study derives viewers' response by extracting key words with opinion mining techniques that divide positive words, neutral words and negative words through customer sentiment analysis. It is found that the success factors of the web-real variety were reduced in appearance fees and production cost, harmony between actual cast members and scenario characters, mobile TV programing, and pre-roll advertising. It is expected that web-real variety broadcasting will increase in value as web contents in the future, and be established as a new genre with the job of 'technical marketer' growing as well.

• Journal of Korean Institute of Industrial Engineers
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• v.43 no.1
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• pp.62-71
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• 2017

In order to attract foreign tourists, it is important to understand what factors on domestic tour spots are critically considered and how they are evaluated after visit. However, most of the researches on tour business have collected information from tourists through survey on a small number of tourists, which leads to inaccurate and biased conclusion. In this paper, we suggest a data-driven methodology to figure out tourists' satisfaction factors and estimate sentiment scores on them. To do so, we collected review comments data from popular web site. Latent dirichlet allocation is employed to extract key factors and elastic net is used to estimate sentiment scores. Then, an aggregated evaluation score is generated by combining the factors and the sentiment scores per topics. Our proposed method can be used to recommend travel schedules with themes and discover new spots.

• The Journal of Engineering Geology
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• v.24 no.4
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• pp.455-461
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• 2014

The Honam-Jeju, Korea-Japan, and Korea-China subsea tunnel construction projects have drawn significant attention since the early 2000s. These subsea tunnels are much deeper than most existing natural shallow sea tunnels linking coastal areas. Thus, the need for developing new technologies for the site selection and construction of deep subsea tunnels has recently emerged, with the launch of a research project titled "Development of Key Subsea Tunnelling Technology" in 2013. A component of this research, an analysis of deep subsea geological structure, is currently underway. A ground investigation, such as a borehole or geophysical investigation, is generally carried out for tunnel design. However, when investigating a potential site for a deep subsea tunnel, borehole drilling requires equipment at the scale of offshore oil drilling. The huge cost of such an undertaking has raised the urgent need for methods to indirectly assess the local geological structure as much as possible to limit the need for repeated borehole investigations. This study introduces an indirect approach for assessing the geological structure of the seafloor through a submarine bathymetry analysis. The ultimate goal here is to develop an automated approach to the analysis of submarine geological structures, which may prove useful in the selection of future deep subsea tunnel sites.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.75-75
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• 1995

Succinic semialdehyde reductase, one of key enzyme of GABA shunt in CNS, is inactivated by o-phthalaldehyde, The inactivation followed pseudo first-order kinetics, and the second-order rate constant for the inactivation process was 28 M$\^$-1/s$\^$-1/ at pH 7.4 and 25$^{\circ}C$. The absorption spectrum(λ$\_$max/=377nm), fluorescence exitation(λ$\_$max/=340nm) and fluorescence emission spectra (λ$\_$max/=409nm) were consistent with the formation of an isoindole derivative in the catalytic site between a cysteine and a lysine residues about 3${\AA}$ apart. The substrate, succinic semialdehyde, did not protect the enzymatic activity against inactivation, whereas the coenzyme, NADPH, protected against o-phthalaldehyde induced inactivation of the enzyme. About 1 isoindole group per moi of the enzyme was formed following complete loss of the enzymatic activity. These results suggest that the amino acid residues of the enzyme participating in reaction with o-phthalaldehyde more likely residues at or near the coenzyme binding site.

• Biomolecules & Therapeutics
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• v.22 no.3
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• pp.232-238
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• 2014

Alzheimer's disease (AD) is the most common neurodegenerative disease without known ways to cure. A key neuropathologic manifestation of the disease is extracellular deposition of beta-amyloid peptide (Ab). Specific mechanisms underlying the development of the disease have not yet been fully understood. In this study, we investigated effects of 4-O-methylhonokiol on memory dysfunction in APP/PS1 double transgenic mice. 4-O-methylhonokiol (1 mg/kg for 3 month) significantly reduced deficit in learning and memory of the transgenic mice, as determined by the Morris water maze test and step-through passive avoidance test. Our biochemical analysis suggested that 4-O-methylhonokiol ameliorated $A{\beta}$ accumulation in the cortex and hippocampus via reduction in beta-site APP-cleaving enzyme 1 expression. In addition, 4-O-methylhonokiol attenuated lipid peroxidation and elevated glutathione peroxidase activity in the double transgenic mice brains. Thus, suppressive effects of 4-O-methylhonokiol on $A{\beta}$ generation and oxidative stress in the brains of transgenic mice may be responsible for the enhancement in cognitive function. These results suggest that the natural compound has potential to intervene memory deficit and progressive neurodegeneration in AD patients.

• Journal of Information Technology Applications and Management
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• v.14 no.2
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• pp.71-96
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• 2007

The objectives of this study are to investigate factors influencing trust on internet shopping mall and to examine whether these factors on trust have differences between Korean and Chinese users. Based on the literature reviews, this study employs six factors in two groups of influencing factors, web-site characteristics and individual characteristics as key determinants of trust in internet shopping mall. Analysis of 470 responses(Korean: 320 users, Chinese: 150 users) of survey questionnaire indicates the following: In terms of the relationship between web-site characteristics factors and the trust, the results showed significant differences between Korean and Chinese samples. For the Korean samples, transaction security and interactivity were the only significant influencing factors. For the Chinese samples, perceived reputation and perceived size were the only significant factors. In terms of individual characteristics factors, the influencing factors were found identical for the samples. Both propensity to trust and familarity were significant factors in both Korean and Chinese samples. Based on the findings, managerial implications are discussed in building user trust in internet shopping mall in Korean and Chinese market.

• The Korean Journal of Ceramics
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• v.6 no.3
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• pp.309-314
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• 2000

In the present study, preliminary experimental results of the change in the properties of perovskite-type oxides caused by the $^{18}O$- exchange have been reported. Two systems were selected for the exchange, (1) $ATiO_3$(A=Ca,Sr,Ba) and (2) manganese perovskite. The dielectric properties of isotope-exchanged $SrTi^{18}O_3$showed a drastic change from a quantum paraelectricity below 3K to ferroelectric-like behavior with a peak at 23K and an enhanced dielectric constant, 35000 at the peak. On the contrary, the $T_c$ for $BaTiO_3$was found to increase by 0.9K. The observed isotope shift of $T_c$ as well as $T_co$ for the manganese perovskites is correlated with the key parameters controlling the lattice such as $Mn^{3+}$ content, average ionic radius of the A-site cation <$r_A$> ad A-site ionic disorder $\sigma^2$.