• The Journal of the Korea Contents Association
• /
• v.15 no.10
• /
• pp.607-615
• /
• 2015

Mosts user of internet and smart phone has certificate, and uses it when money transfer, stock trading, on-line shopping, etc. Mosts user stores certificate in a hard disk drive of PC, or the external storage medium. In particular, the certification agencies are encouraged for user to store certificate in external storage media such as USB memory rather than a hard disk drive. User think that the external storage medium is safe, but when it is connect to a PC, certificate may be copied easily, and can be exposed to hackers through malware or pharming site. Moreover, if a hacker knows the user's password, he can use user's certificate without restrictions. In this paper, we suggest secure management scheme of the private key file using a password of the encrypted private key file, and a USB Memory's hardware information. The private key file is protected safely even if the encrypted private key file is copied or exposed by a hacker. Also, if the password of the private key file is exposed, USB Memory's container ID, additional authentication factor keeps the private key file safe. Therefore, suggested scheme can improve the security of the external storage media for certificate.

• Journal of Veterinary Science
• /
• v.24 no.3
• /
• pp.44.1-44.17
• /
• 2023

Background: Antibiotic resistance is a significant public health concern around the globe. Antimicrobial peptides exhibit broad-spectrum and efficient antibacterial activity with an added advantage of low drug resistance. The higher water content and 3D network structure of the hydrogels are beneficial for maintaining antimicrobial peptide activity and help to prevent degradation. The antimicrobial peptide released from hydrogels also hasten the local wound healing by promoting epithelial tissue regeneration and granulation tissue formation. Objective: This study aimed at developing sodium alginate based hydrogel loaded with a novel antimicrobial peptide Chol-37(F34-R) and to investigate the characteristics in vitro and in vivo as an alternative antibacterial wound dressing to treat infectious wounds. Methods: Hydrogels were developed and optimized by varying the concentrations of crosslinkers and subjected to various characterization tests like cross-sectional morphology, swelling index, percent water contents, water retention ratio, drug release and antibacterial activity in vitro, and Pseudomonas aeruginosa infected wound mice model in vivo. Results: The results indicated that the hydrogel C proved superior in terms of cross-sectional morphology having uniformly sized interconnected pores, a good swelling index, with the capacity to retain a higher quantity of water. Furthermore, the optimized hydrogel has been found to exert a significant antimicrobial activity against bacteria and was also found to prevent bacterial infiltration into the wound site due to forming an impermeable barrier between the wound bed and external environment. The optimized hydrogel was found to significantly hasten skin regeneration in animal models when compared to other treatments in addition to strong inhibitory effect on the release of pro-inflammatory cytokines (interleukin-1β and tumor necrosis factor-α). Conclusions: Our results suggest that sodium alginate -based hydrogels loaded with Chol-37(F34-R) hold the potential to be used as an alternative to conventional antibiotics in treating infectious skin wounds.

• The Plant Pathology Journal
• /
• v.25 no.1
• /
• pp.70-76
• /
• 2009

The rhizobacteria of Panax notoginseng were isolated from six sites in Yanshan, Maguan and Wenshan Counties, Yunnan Province of China, and their antagonistic activity against P. notoginseng root-rot fungal pathogens was determined. Of the 574 rhizobacteria isolated, 5.8% isolates were antagonistic in vitro to at least one of the five pathogens, Cylindrocarpon didynum, Fusarium solani, Phytophthora cactorum, Phoma herbarum, and Rhizoctonia solani. The number of rhizo bacteria and the number that inhibited fungi differed depending on sampling sites and isolation methods. Rhizobacteria isolated from the site in Yanshan and Maguan showed more antagonistic effect than them in Wenshan. Heat treatment of rhizosphere soil at $80^{\circ}C$ for 20 min scaled the antagonists up to 14.0%. Antagonistic bacteria in the roots proportioned 3.9% of the total isolates. The most antagonistic isolates 79-9 and 81-4 are Bacillus subtilis based on their 168 rDNA sequence and biochemical and physiological characteristics. Identification and evaluation of antagonistic bacteria against P. notoginseng root-rot pathogens in the main planting areas improved our understanding of their distribution in rhizosphere soil. Furthermore these results indicated that the interactions between biocontrol agent and soil microbes should be seriously considered for the successful survival and biocontrol efficacy of the agents in soil.

• The KIPS Transactions:PartC
• /
• v.15C no.5
• /
• pp.375-382
• /
• 2008

As the use of Internet and information communication technology is being generalized, the SSL protocol is essential in Internet because the important data should be transferred securely. While the SSL protocol is designed to defend from active attack such as message forgery and message alteration, the cipher suite setting can be easily modified. If the attacker draw on a malfunction of the client system and modify the cipher suite setting to the symmetric key algorithm which has short key length, he should eavesdrop and cryptanalysis the encrypt data. In this paper, we examine the domestic web site whether they generate the security session in the symmetric key algorithm which has short key length and propose the solution of the cipher suite setting problem.

• BMB Reports
• /
• v.47 no.4
• /
• pp.233-238
• /
• 2014

Polypyrimidine tract-binding protein 1 (PTBP1) and its brain-specific homologue, PTBP2, are associated with pre-mRNAs and influence pre-mRNA processing, as well as mRNA metabolism and transport. They play important roles in neural differentiation and glioma development. In our study, we detected the expression of the two proteins in glioma cells and predicted that they may be sumoylated using SUMOplot analyses. We confirmed that PTBP1 and PTBP2 can be modified by SUMO1 with co-immunoprecipitation experiments using 293ET cells transiently co-expressing SUMO1 and either PTBP1 or PTBP2. We also found that SUMO1 modification of PTBP2 was enhanced by Ubc9 (E2). The mutation of the sumoylation site (Lys137) of PTBP2 markedly inhibited its modification by SUMO1. Interestingly, in T98G glioma cells, the level of sumoylated PTBP2 was reduced compared to that of normal brain cells. Overall, this study shows that PTBP2 is posttranslationally modified by SUMO1.

• Molecules and Cells
• /
• v.40 no.9
• /
• pp.643-654
• /
• 2017

Autophagy is a degradation pathway in eukaryotic cells in which aging proteins and organelles are sequestered into double-membrane vesicles, termed autophagosomes, which fuse with vacuoles to hydrolyze cargo. The key step in autophagy is the formation of autophagosomes, which requires different kinds of vesicles, including COPII vesicles and Atg9-containing vesicles, to transport lipid double-membranes to the phagophore assembly site (PAS). In yeast, the cis-Golgi localized t-SNARE protein Sed5 plays a role in endoplasmic reticulum (ER)-Golgi and intra-Golgi vesicular transport. We report that during autophagy, sed5-1 mutant cells could not properly transport Atg8 to the PAS, resulting in multiple Atg8 dots being dispersed into the cytoplasm. Some dots were trapped in the Golgi apparatus. Sed5 regulates the anterograde trafficking of Atg9-containing vesicles to the PAS by participating in the localization of Atg23 and Atg27 to the Golgi apparatus. Furthermore, we found that overexpression of SFT1 or SFT2 (suppressor of sed5 ts) rescued the autophagy defects in sed5-1 mutant cells. Our data suggest that Sed5 plays a novel role in autophagy, by regulating the formation of Atg9-containing vesicles in the Golgi apparatus, and the genetic interaction between Sft1/2 and Sed5 is essential for autophagy.

• Bulletin of the Korean Chemical Society
• /
• v.35 no.7
• /
• pp.2013-2018
• /
• 2014

The adsorption and decomposition of trimethylene oxide ($C_3H_6O$) molecule on the Al(111) surface were investigated by the generalized gradient approximation (GGA) of density functional theory (DFT). The calculations employed a supercell ($6{\times}6{\times}3$) slab model and three-dimensional periodic boundary conditions. The strong attractive forces between $C_3H_6O$ molecule and Al atoms induce the C-O bond breaking of the ring $C_3H_6O$ molecule. Subsequently, the dissociated radical fragments of $C_3H_6O$ molecule oxidize the Al surface. The largest adsorption energy is about -260.0 kJ/mol in V3, V4 and P2, resulting a ring break at the C-O bond. We also investigated the decomposition mechanism of $C_3H_6O$ molecules on the Al(111) surface. The activation energies ($E_a$) for the dissociations V3, V4 and P2 are 133.3, 166.8 and 174.0 kJ/mol, respectively. The hcp site is the most reactive position for $C_3H_6O$ decomposing.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.4
• /
• pp.431-439
• /
• 2014

We report the construction of two Bacillus subtilis expression vectors, pBNS1/pBNS2. Both vectors are based on the strong promoter P43 and the ampicillin resistance gene expression cassette. Additionally, a fragment with the Shine-Dalgarno sequence and a multiple cloning site (BamHI, SalI, SacI, XhoI, PstI, SphI) were inserted. The coding region for the amyQ (encoding an amylase) signal peptide was fused to the promoter P43 of pBNS1 to construct the secreted expression vector pBNS2. The applicability of vectors was tested by first generating the expression vectors pBNS1-GFP/pBNS2-GFP and then detecting for green fluorescent protein gene expression. Next, the mannanase gene from B. pumilus Nsic-2 was fused to vector pBNS2 and we measured the mannanase activity in the supernatant. The mannanase total enzyme activity was 8.65 U/ml, which was 6 times higher than that of the parent strain. Our work provides a feasible way to achieve an effective transformation system for gene expression in B. subtilis and is the first report to achieve B. pumilus mannanase secretory expression in B. subtilis.

• Wind and Structures
• /
• v.17 no.1
• /
• pp.43-68
• /
• 2013

The fluctuating wind induced vibration is one of the most important factors which has been taken into account in the design of long-span bridge due to the low stiffness and low natural frequency. Field measurement characteristics of sustained wind on structure site can provide accurate wind load parameters for wind field simulation and structural wind resistance design. As a suspension bridge with 1490 m main span, the Runyang Suspension Bridge (RSB) has high sensitivity to fluctuating wind. The simultaneous and continuously wind environment field measurement both in mid-span and on tower top is executed from 2005 up to now by the structural health monitoring system installed on this bridge. Based on the recorded data, the wind characteristic parameters, including mean wind speed, wind direction, the turbulence intensity, the gust factors, the turbulence integral length, power spectrum and spatial correlation, are analyzed in detail and the coherence functions of those parameters are evaluated using statistical method in this paper. The results indicate that, the turbulence component of sustain wind is larger than extremely strong winds although its mean wind speed is smaller; the correlation between turbulence parameters is obvious; the power spectrum is special and not accord with the Simiu spectrum and von Karman spectrum. Results obtained in this study can be used to evaluate the long term reliability of the Runyang Suspension Bridge and provide reference values for wind resistant design of other structures in this region.

• Journal of Electrical Engineering and Technology
• /
• v.9 no.4
• /
• pp.1385-1393
• /
• 2014

Gas insulated switchgear (GIS) is widely used in the power systems, however, the contacts overheating of the inside circuit breaker or disconnector may be a potential cause of developing accidents. As the temperature of the contacts cannot be directly acquired due to existence of the metallic shield, an infrared sensor is adopted to directly measure the temperature of the shield and then the contacts temperature can be indirectly obtained by data fitting, based on which the on-line temperature monitoring technology specifically for GIS contacts based on infrared sensing is proposed in this paper. A real GIS test platform is constructed and experimental studies are carried out to account for the influential factors that affect the accuracy of the infrared temperature measurement. A heat transfer model of the GIS module is also developed, together with experimental studies, the nonlinear temperature relationship among the contacts, the metallic shield and the environment based on a neural network algorithm is established. Finally, an integrated on-line temperature monitoring system for the GIS contacts is developed for on-site applications.