• Asian-Australasian Journal of Animal Sciences
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• 제24권12호
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• pp.1718-1728
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• 2011

Two experiments were conducted to investigate the in vitro ability of keratinase to hydrolyze soybean glycinin and ${\beta}$-conglycinin and to evaluate the in vivo effects of keratinase when included in corn-soybean diets with different levels of crude protein and fed to nursery pigs. In experiment 1, a saturated keratinase solution (1 ml) was added to two blank controls of either glycinin or ${\beta}$-conglycinin resulting in the hydrolysis of 94.74% glycinin and 88.89% ${\beta}$-conglycinin. In experiment 2, 190 pigs (8.3${\pm}$0.63 kg BW) were allotted to one of four treatments in a 2${\times}$2 factorial arrangement on the basis of body weight, and sex was balanced among the pens. The effects of crude protein (19 vs. 22%) and keratinase (0 vs. 0.05%) were studied. Each treatment was applied to six pens with seven (two pens) or eight pigs per pen. Pigs were fed the experimental diets for 21 d. Weight gain and feed conversion ratio were improved (p<0.05) with keratinase supplementation while feed intake was reduced (p<0.05). Keratinase supplementation increased (p<0.05) the apparent total tract digestibility of dry matter, energy, crude protein and phosphorus. Keratinase supplementation also increased n-butyric acid in the cecum and colon, lactobacilli and total anaerobe counts in the colon as well as the ratio of villus height to crypt depth in the ileum. Additionally, fecal score, ammonia nitrogen and branch chain volatile fatty acids in the colon, E. coli and total aerobe counts in the colon, crypt depth in the jejunum and ileum as well as serum interleukin-1 and interleukin-6 concentrations were also decreased (p<0.05) by keratinase supplementation. A reduction in dietary crude protein decreased (p<0.05) colon ammonia nitrogen concentration and cecal propionic acid and branch chain volatile fatty acid concentrations. In addition, cecal E. coli counts, colon total anaerobe counts, ileal crypt depth, and serum interleukin-1 and interleukin-6 concentrations were also decreased (p<0.05) with the reduction of dietary crude protein. With the exception of fecal scores, there were no significant interactions between crude protein and keratinase. This study provides evidence that dietary keratinase supplementation improved nursery pig performance by improving intestinal morphology and ecology, thus improving nutrient digestibility and alleviating the inflammatory response.

• Journal of Microbiology and Biotechnology
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• 제25권7호
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• pp.1101-1107
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• 2015

The role of CRE1 in a thermophilic fungus, Myceliophthora thermophila ATCC42464, was studied using RNA interference. In the cre1-silenced strain C88, the filter paper hydrolyzing activity and β-1,4-endoglucanase activity were 3.76-, and 1.31-fold higher, respectively, than those in the parental strain when the strains were cultured in inducing medium for 6 days. The activities of β-1,4-exoglucanase and cellobiase were 2.64-, and 5.59-fold higher, respectively, than those in the parental strain when the strains were cultured for 5 days. Quantitative reverse-transcription polymerase chain reaction showed that the gene expression of egl3, cbh1, and cbh2 was significantly increased in transformant C88 compared with the wild-type strain. Therefore, our findings suggest the feasibility of improving cellulase production by modifying the regulator expression, and an attractive approach to increasing the total cellulase productivity in thermophilic fungi.

• 한국정보시스템학회지:정보시스템연구
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• 제20권2호
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• pp.109-134
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• 2011

The purpose of this paper is to present an overview of the characteristics of the e-sports business at a crucial point in the era of information and knowledge. That is, an investigation was conducted surrounding the key success factors of e-sports as a newly growing industry, and the relationship between the key success factors and the usage effect. In order to do this, the key success factors were extracted from interviews with e-sports experts in conjunction with a survey that was conducted to gather empirical data. The key points of the statistical analysis of the survey are as follows. First, the success factors of a policy do not have a significant effect on the usage. Second, some administrative success factors of the e-sports business include the distribution of e-sports in the form of franchise chain, the establishment of policies to prevent the excellent members of e-sports from seceding, making a good impression as a high-class sport, the frequent notice of the break-even point, and educating people on how to use the e-sports machine. Third, the development and distribution of excellent e-sports software was analyzed as a technical success factor of the e-sports business. Finally, there is no doubt that results of this study can be used to facilitate the growth of the e-sports business as a new industry related to the IT industry.

• Bulletin of the Korean Chemical Society
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• 제31권8호
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• pp.2211-2214
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• 2010

The whole plant of Euphorbia helioscopia is an important traditional Chinese medicine. Fom its BuOH soluble extract, one new lactam (1), three new terpenoids (2-4) including a new naturally occurring compound, and three known compounds were isolated. Their structures were identified by spectroscopic evidences. In particular, the absolute configurations of side chain of compounds 1 and 2 were determined using computational methods.

• Bulletin of the Korean Chemical Society
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• 제35권6호
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• pp.1675-1680
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• 2014

A peroxopolyoxotungsten-based ionic hybrid was synthesized by anion-change of peroxopolyoxometalate (POM) $PW_4O{_{24}}^{3-}$ with dicationic long-chain alkyl imidazolium ionic liquids. The characterization was conducted by FT-IR, TGA, $^1H$-NMR and CHN Elemental analyses. Its catalytic performance was evaluated by the epoxidation of soybean oil with $H_2O_2$ under solvent-free condition, including testing of organic cations influence, catalytic reusability and reaction conditions. The catalyst was proved to be a highly efficient recyclable catalyst for epoxidation of various vegetable oils with $H_2O_2$, showing high $H_2O_2$ utilization efficiency, high catalytic activity, convenient recovery and good reuse ability.

• Bulletin of the Korean Chemical Society
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• 제35권2호
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• pp.392-396
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• 2014

New organic-inorganic composite of 2,4-dihydroxybenzophenone (BP-1) encapsulation into dodecylbenzenesulfonate (DBS) modified layered double hydroxide (LDH) was successfully prepared. The surface, structural, thermal and absorption properties of the BP-1/DBS-LDH nanohybrid was characterized by BET analysis, scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TG) and diffuse reflectance UV-Vis absorbance spectra (DRUV-vis). The interlayer configuration of composite and the adsorption mechanism of BP-1 on MgAl-DBS-LDH were discussed. It was suspected that DBS anions located in the form of monolayer arrangement with a $75^{\circ}$ anti parallel angle between dodecylbenzenesulfonate chain axis. The diffuse reflectance UV-Vis absorbance results revealed that the UV absorbing wavelength of BP-1/DBS-LDH evidently extends to about 400 nm, which shows that the BP-1/DBS-LDH has the potential application as a UV absorber.

• 한국세라믹학회지
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• 제53권6호
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• pp.665-669
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• 2016

$TiO_2/BiVO_4$ layered films were prepared by sol-gel and spin coating methods. X-ray diffraction (XRD), scanning electron microscopy (SEM) and Uv-vis spectroscopy were used to investigate the crystal structure, morphology and ultraviolet-visible absorption of the $TiO_2/BiVO_4$ films. The photocatalytic activity of the prepared films was inspected according to the degradation of methylene blue. The results show that the prepared films present a net chain structure; the absorption band edge had obvious red shift. The degradation of the methylene blue solution was about 80% after 300 mins using $TiO_2/BiVO_4$ layered films under visible light, which was stronger than when using only pure $TiO_2$ film and $BiVO_4$ film.

• 한국컴퓨터정보학회논문지
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• 제11권3호
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• pp.57-66
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• 2006

본 논문에서는 IETF CAT Working Group에서 발표한 PKINIT기반의 인증서비스를 향상시킨 Multi_Kerberos 인증 메커니즘을 제안한다. PKINIT기반의 X.509, DS/DNS를 적용하여 영역간의 서비스를 제공하는 인증과 키 교환방식으로 DNS를 통해 외부영역의 위치를 탐색하고 X.509 디렉터리 인증 시스템을 적용, 영역간 체인(CertPath)으로 DNS 서버로부터 공개키를 획득하여 다른 영역을 인증하도록 하였다. 검증서버를 활용하여 인증서 경로생성 및 구축, 세션키의 복구, 인증서 기반의 키 관리를 포함한 상호영역(cross realm)에 대한 효율적인 인증서비스를 지원하는 메커니즘을 제안하였다. 이에 통신상의 절차를 감소시키는 효과와 인증절차의 간소화를 가지는 Multi_Kerberos 시스템을 설계하였다.

• 한국정보과학회논문지:정보통신
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• 제28권3호
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• pp.309-320
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• 2001

본 논문은 이동 에이전트 시스템에서 발생할 수 있는 보안 문제, 특히 악의를 가진 에이전트 서버로부터 에이전트의 데이타를 보호하기 위한 일회용 에이전트 키 생성시스템을 제안한다. 제안된 일회용 에이전트 키 시스템은 일방향(one-way) 해쉬함수와 연결고리(coupler) 개념을 병용한다. 먼저 일방향 함수는 에이전트 데이타의 비밀성과 무결성을 보장하기 위해서 중요한 역할을 하며, 연결고리는 에이전트의 데이타를 보호하기 위해서 연속되는 암호화키들 사이의 일정한 연관관계(key chain)를 설정하기 위해서 사용된다. 즉 모든 에이전트 키들은 한 방향의 연결고리를 형성하게 된다. 위와 같이 일회용 에이전트 키 생성시스템의 두 가지 특징은 에이전트 소유자(처음 에이전트를 생성한 사용자)만이 에이전트가 순회하면서 수집한 모든 데이타를 복호화 할 수 있도록 하며 악의를 가진 다른 사용자로부터 에이전트 데이타를 보호할 수 있다.

• 한국통신학회논문지
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• 제32권1B호
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• pp.11-16
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• 2007

본 논문에서는 이동 애드혹 망을 위해 시공간 방식에 근거한 보안 라우팅 프로토콜의 설계와 성능을 기술한다. 제안된 시공간 방식은 시간영역에서는 출발지와 목적지 간에 키 배포를 수행하고, 또한 공간영역에서는 경로 상에서의 침입 탐지를 수행하기 때문에 붙여진 이름이다. 데이터 인증을 위해 효율성이 높은 대칭키 방식을 사용하고, 비밀 키는 출발지에서 목적지로 시간차를 이용하여 배포되어 진다. 또한, 단방향의 해쉬체인을 공간측면에서 홉단위로 형성함으로써, 침입자나 포섭된 노드가 라우팅 정보를 조작하는 것을 방지한다 제안된 라우팅 프로토콜의 성능을 평가하기 위해 ns-2 시뮬레이션을 통하여 같은 조건하에서 기존의 AODV와 비교한 결과, 우리가 제안한 프로토콜이 보안 기능을 제공하면서도 동시에 지연시간과 처리율 측면에서 기존의 AODV와 유사한 성능을 보여주고 있다.