• Korean Journal of Radiology
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• v.24 no.9
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• pp.871-889
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• 2023

C-X-C motif chemokine receptor 4 (CXCR4) plays a key role in various physiological functions, such as immune processes and disease development, and can influence angiogenesis, proliferation, and distant metastasis in tumors. Recently, several radioligands, including peptides, small molecules, and nanoclusters, have been developed to target CXCR4 for diagnostic purposes, thereby providing new diagnostic strategies based on CXCR4. Herein, we focus on the recent research progress of CXCR4-targeting radioligands for tumor diagnosis. We discuss their application in the diagnosis of hematological tumors, such as lymphomas, multiple myelomas, chronic lymphocytic leukemias, and myeloproliferative tumors, as well as nonhematological tumors, including tumors of the esophagus, breast, and central nervous system. Additionally, we explored the theranostic applications of CXCR4-targeting radioligands in tumors. Targeting CXCR4 using nuclear medicine shows promise as a method for tumor diagnosis, and further research is warranted to enhance its clinical applicability.

• Horticultural Science & Technology
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• v.34 no.2
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• pp.314-323
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• 2016

A transient ihpRNA-induced gene silencing system based on Agrobacterium-mediated injection infiltration has been established to evaluate candidate genes involved in proanthocyanidin (PAs) biosynthesis in persimmon (Diospyros kaki Thunb.). We chose DkPDS (phytoene desaturase) as a gene-silencing target to evaluate the newly developed transient gene silencing system. Our qRT-PCR analysis indicated that two ihpRNA constructs (pHG-PDS5' and pHG-PDS3') targeted DkPDS, which also led to significantly reduce expression of DkPDS in 'Mopanshi' persimmon leaves. To further confirm the reliability of the system, we successfully utilized it for DkLAR (leucoanthocyanidin reductase) gene silencing. The expression levels of DkLAR in 'Mopanshi' and 'Eshi 1' leaves were ca. 6-fold and ca. 5-fold lower than those in leaves harboring empty vector (pHG-GFP), respectively. DMACA (4-dimethylaminocinnamaldehyde) staining and the Folin-Ciocalteau assay showed that the accumulation of PAs was markedly inhibited in 'Mopanshi', 'Eshi 1' and 'Youhou' leaves. These results indicate that DkLAR plays an important role in the accumulation of PAs in persimmon. The transient ihpRNA-induced gene silencing method developed in this study is a highly efficient and useful tool for functional analysis of persimmon genes involved in PA biosynthesis.

• ETRI Journal
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• v.40 no.1
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• pp.39-50
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• 2018

More people prefer using rail traffic for travel or for commuting owing to its convenience and flexibility. The railway scenario has become an important communication scenario in the fifth generation era. The communication system should be designed to support high-data-rate demands with seamless connectivity at a high mobility. In this paper, the channel characteristics are studied and modeled for the railway tunnel scenario with straight and curved route shapes. On the basis of measurements using the "Mobile Hotspot Network" system, a three-dimensional ray tracer (RT) is calibrated and validated for the target scenarios. More channel characteristics are explored via RT simulations at 25.25 GHz with a 500-MHz bandwidth. The key channel parameters are extracted, provided, and incorporated into a 3rd-Generation-Partnership-Project-like stochastic channel generator. The necessary channel information can be practically realized, which can support the link-level and system-level design of the communication system in similar scenarios.

• IMMUNE NETWORK
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• v.22 no.3
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• pp.21.1-21.21
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• 2022

As far the current severe coronavirus disease 2019 (COVID-19), respiratory disease is still the biggest threat to human health. In addition, infectious respiratory diseases are particularly prominent. In addition to killing and clearing the infection pathogen directly, regulating the immune responses against the pathogens is also an important therapeutic modality. Sirtuins belong to NAD+-dependent class III histone deacetylases. Among 7 types of sirtuins, silent information regulator type-1 (SIRT1) played a multitasking role in modulating a wide range of physiological processes, including oxidative stress, inflammation, cell apoptosis, autophagy, antibacterial and antiviral functions. It showed a critical effect in regulating immune responses by deacetylation modification, especially through high-mobility group box 1 (HMGB1), a core molecule regulating the immune system. SIRT1 was associated with many respiratory diseases, including COVID-19 infection, bacterial pneumonia, tuberculosis, and so on. Here, we reviewed the latest research progress regarding the effects of SIRT1 on immune system in respiratory diseases. First, the structure and catalytic characteristics of SIRT1 were introduced. Next, the roles of SIRT1, and the mechanisms underlying the immune regulatory effect through HMGB1, as well as the specific activators/inhibitors of SIRT1, were elaborated. Finally, the multitasking roles of SIRT1 in several respiratory diseases were discussed separately. Taken together, this review implied that SIRT1 could serve as a promising specific therapeutic target for the treatment of respiratory diseases.

• IEMEK Journal of Embedded Systems and Applications
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• v.12 no.3
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• pp.159-167
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• 2017

Software safety is a key issue in embedded system of automotive and aviation industries. Various software testing approaches have been proposed to achieve software safety like ISO26262 Part 6 in automotive environment. In spite of one of the classic and basic approaches, stack memory is hard to estimating exactly because of uncertainty of target code generated by compiler and complex nested interrupt. In this paper, we propose an approach of analyzing the maximum stack usage statically from target binary code rather than the source code that also allows nested interrupts for determining the exact stack memory size. In our approach, determining maximum stack usage is divided into three steps: data extraction from ELF file, construction of call graph, and consideration of nested interrupt configurations for determining required stack size from the ISR (Interrupt Service Routine). Experimental results of the estimation of the maximum stack usage shows proposed approach is helpful for optimizing stack memory size and checking the stability of the program in the embedded system that especially supports nested interrupts.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.550-557
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• 2010

Escherichia coli (E. coli) heat-labile enterotoxin B subunit (LTB) was regarded as one of the most powerful mucosal immunoadjuvants eliciting strong immunoresponse to coadministered antigens. In the research, the high-level secretory expression of functional LTB was achieved in P. pastoris through high-density fermentation in a 5-1 fermentor. Meanwhile, the protein was expressed in E. coli by the way of inclusion body, although the gene was cloned from E. coli. Some positive yeast and E. coli transformants were obtained respectively by a series of screenings and identifications. Fusion proteins LTB-6$\times$His could be secreted into the supernatant of the medium after the recombinant P. pastoris was induced by 0.5% (v/v) methanol at $30^{\circ}C$, whereas E. coli transformants expressed target protein in inclusion body after being induced by 1 mM IPTG at $37^{\circ}C$. The expression level increased dramatically to 250-300 mg/l supernatant of fermentation in the former and 80-100 mg/l in the latter. The LTB-6$\times$His were purified to 95% purity by affinity chromatography and characterized by SDS-PAGE and Western blot. Adjuvant activity of target protein was analyzed by binding ability with GMI gangliosides. The MW of LTB-6$\times$His expressed in P. pastoris was greater than that in E. coli, which was equal to the expected 11 kDa, possibly resulted from glycosylation by P. pastoris that would enhance the immunogenicity of co-administered antigens. These data demonstrated that P. pastoris producing heterologous LTB has significant advantages in higher expression level and in adjuvant activity compared with the homologous E. coli system.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.6
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• pp.2441-2451
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• 2012

This paper presents the design of a control system for improving performance of a washer fluid heating system (WFHS) which is capable of removing frost, ice, snow and/or other debris from windshield. First, for the WFHS, a modeling process is described, and the extraction of characteristic parameters of the model are made by experimental studies. Design variables that affect on performance of the WFHS are also presented. Secondly, a control system is proposed for improving heating performance of the WFHS, and its performance is verified through experiments. The key feature of the proposed control system is to regulate the current of a booster converter input to the WFHS up to the target value that is set to guarantee heating performance. Target current is calculated by using initial temperature value and employing the mathematical model derived in the paper. Computer simulation and experimental results show that the proposed control system can perform heating operations in a way to satisfy per-determined target performance of the WFHS.

• Journal of KIISE:Computing Practices and Letters
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• v.12 no.3
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• pp.160-169
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• 2006

The goal of this paper is to propose a way to register agents so that makes users or autonomousagents to find target agents more conveniently and efficiently. In order to achieve this goal, we first survey the key technologies such as UDDI, ebXML, FIPA DF. As the results, we designed a new agent legistry/repository system based on ebXML technologies. We introduce our agent/repository system for wide area network and describe how to use our system for agent registering and searching target agents for communications. Finally, we give an example to illustrate a typical scenario in which user receives the results from our proposed agent registry/repository system and evaluate its characteristics in terms of advantage.

• Journal of the Korean Society for Railway
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• v.15 no.3
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• pp.278-285
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• 2012

The Service Availability (SA) in the viewpoint of passenger is used as the key performance indicator (KPI) of quality of service in Light Rail Transit (LRT) Public-Private Partnerships projects. But there are many disputes on the target value of SA because of the lack of experience in SA. The target value of SA should be set at an early stage of the project to be specified on the system specifications and operation plan. Therefore, this paper developed the quantitative prediction model of SA to set the reasonably achievable target value of SA at an early stage of the LRT project. Also this paper analyzed the relationship and differentiation of SA and Train Punctuality (TP) that is mostly compared with SA.

• Journal of Korean Society on Water Environment
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• v.33 no.1
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• pp.70-77
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• 2017

Total Pollution Load Control (TPLC) is a system for managing the discharge load assigned by satisfying the Target Water Quality (TWQ) in Standard Flow Conditions (SFC). TWQ for a between Metropolitan Cities/Dos Specified (Cites/Dos TWQ) is very important to be the basis of each Unit Watershed TWQ. The purpose of this study was to establish a rational and scientific 'Calculation Metohd of Cites/Dos TWQ'. A methodology for the 3rd phase 'Cites/Dos TWQ' was proposed in this study based on review of the past phase (1rd and 2rd) 'Cites/Dos TWQ' in nakdong river. And utilized water quality model to estimate 3rd phase 'Cites/Dos TWQ' The allocation method of individual discharge sources are important for estimating 'Cites/Dos TWQ' In this case, the key point of the method of calculating the total allowable individual sources is the balance of the equity and the efficiency between individual sources of reduced pollutants. Thus, water quality shall be determined with regard to the current emission levels, the reduction capacity and the technical possibilities of individual sources. We estimate 3rd phase 'Cites/Dos TWQ' according to the 'Calculation Method of Cites/Dos TWQ'.