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Increment of fructan biosynthesis in rice by transformation of 1-sst and 1-fft genes isolated from jerusalem artichoke (Helianthus tuberosus L.) (돼지감자 유래 1-sst와 1-fft 유전자의 형질전환 발현에 의한 벼의 fructan 생합성 증진)

  • Kang, Kwon-Kyoo;Song, Beom-Heon;Lee, Gyong-A;Lee, Hye-Jung;Park, Jin-Ha;Jung, Yu-Jin;Cho, Yong-Gu
    • Journal of Plant Biotechnology
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    • v.37 no.1
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    • pp.102-109
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    • 2010
  • Fructan has been found to accumulate in various tissues during periods when light levels increased carbon fixation where low temperatures reduced growth rates while photosynthesis continued. In this study, we have cloned 1-sucrose:sucrose fructosyl transferase(1-sst) and 1-fructan: fructan fructosyl transferase (1-fft, a key enzyme for the synthesis of fuctan) from Jerusalem Artichoke (Helianthus tuberosus L.). The recombinant vector with 1-sst and 1-fft has been constructed under the control of 35S promoter of KJGV-B2 vector and transgenic plants obtained by Agrobacterium tumefaciens LBA4404. PCR analysis carried out on the putative transgenic plants for amplification of the coding region of specific gene (1-sst, 1-fft), and HPT genes. Transgenic lines carrying of 1-sst and 1-fft were confirmed for integration into the rice genome using Southern blot hybridization and RT-PCR. The transgenic plants in $T_2$ generation were selected and expression pattern analysis revealed that 1-sst and 1-fft were stable. This analysis confirmed the presence of low-molecular-weight fructan in the seedling of the transgenic rices. Therefore, cold tolerance and carbohydrate metabolism will be possible to develop resistant plants using the transgenic rice.

An Experimental Study of Wave Overtopping Characteristics on the Structure for Wave Overtopping Power Generating System (월파형 파력발전구조물의 월파 특성에 관한 실험적 연구)

  • Shin, Seung-Ho;Hong, Key-Yong
    • Journal of Navigation and Port Research
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    • v.30 no.8 s.114
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    • pp.649-655
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    • 2006
  • Waves progressing into the coastal area can be amplified, swashed and overtopped by a wave overtopping control structure, and it converts the kinetic energy of the waves to the potential energy with a hydraulic head above the mean sea level by conserving the overflow in a reservoir. Then the potential energy in the form of hydraulic head can be converted to electric power utilizing extremely low-head hydraulic turbine. This study aims to find the most optimal shape of wave overtopping structure which maximizes overtopping volume rate of sea water. Laboratory experiments for the performance evaluation of wave overtopping control structures were carried out in three dimensional wave tank, and the three dimensional structure models with planar wave concentration shapes(B/b) were manufactured into five classes, which were optimized by cross sectional parameters of the structure, ie, length of ramp(l), gradient of inclined ramp($cot{\phi}$) and freeboard height of the wave overtopping structure($h_e$) proposed by Shin and Hong(2005). The wave overtopping discharges were investigated with 20 incident wave conditions and wave directions of $0^{\circ},\;15^{\circ},\;30^{\circ}$.

An Experimental Study on Blade Deformation of Coaxial Rotor System Using SPR(Stereo Pattern Recognition) Technique (SPR(Stereo Pattern Recognition) 기법을 이용한 동축 로터 블레이드의 변형에 대한 실험적 연구)

  • Yoo, Chanho;Yoon, Byung-Il;Chae, Sanghyun;Kim, Do-Hyung;Kim, Deog-Kwan
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.48 no.8
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    • pp.597-609
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    • 2020
  • These days, the coaxial rotor system is used for various purposes like UAVs, Mars exploration helicopters, and the next-generation high-speed rotorcraft. A number of research projects on aerodynamic performance of rotor systems, including the coaxial configuration have been made previously. On the contrary, research on rotor blade deformation has been mainly carried out regarding the single rotor system, where such effort has not been enough on the coaxial system. Nonetheless, in case of the coaxial system, blade deformation analysis is much more important because of the complex air flow around the rotors, and that the distance between the two rotors is a key factor affects aerodynamic performance of the entire system. For these reasons, an experimental study on rotor blade deformation of the coaxial system was conducted using the Stereo Pattern Recognition(SPR) technique, one of the state-of-the-art of photogrammetry method. In this research, a small-scale coaxial rotor test stand designed by Korea Aerospace Research Institute(KARI) was used. With the same test stand, performance of the coaxial configuration had been studied before the experimental study on blade deformation, in order to find the relation between performance and blade deformation of the rotor system. Results of the performance test and the deformation study are presented in this article.

Life Cycle Assessment of Part Reuse/Recycling in the End-of-Life Stage of Personal Computers (부품 재사용 여부에 따른 폐컴퓨터에 대한 전과정평가(LCA))

  • Lim, Hyeong-Soon;Yang, Yun-Hee;Song, Jun-Il;Lee, Kun-Mo
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.5
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    • pp.494-500
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    • 2006
  • Life Cycle Assessment(LCA) is an environmental assessment tool for evaluating environmental burdens associated with products, processes and activities from the raw material acquisition stage to the end-of-life stage. End-of-life stage as well as other processes requires a reliant database in order to increase the confidence in the LCA results. In this study, the flow of Personal Computer(as PC) in the end-of-life stage was examined and the database of two scenarios has been established, i.e. one is part reuse and the other is no part reuse, in the end-of-life phase of PC. Also, key environmental issues were identified by carrying out LCA on a PC in the end-of-life phase for eight environmental impact categories. The 'ozone layer depletion' contributes the highest environmental impact due to generation of $Cl_2$ gas during the incineration of waste plastics. In addition, the scenario 1(part reuse) is more environmentally sound than the scenario 2(no part reuse) when comparing two scenarios.

Synthesis of a series of cis-diamminaedichloro-platinum (II) Complexes Linked to Uracil and Uridine as Candidate An-titumor Agents.

  • Kim, Jack-C.;Kim, Mi-Hyang;Kim, Seon-Hee;Choi, Soon-Kyu
    • Archives of Pharmacal Research
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    • v.18 no.6
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    • pp.449-453
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    • 1995
  • The search for patinum (II)-based compounds with improved therapeutic properties was prompted to design and synthesize a new family of water-soluble, third generation cis-diamminedichlorplatinum (II) complexes linked to uracil and uridine. Six heretofore undescribed uracil and uridine-platinum (II) complexes are ; [N-(2-aminoethyl)uracil-5-carboxamide]dichloroplatinum (II)(3a), [N-2(2-aminoethyl)uracil-6-carboxmide]dichloroplatinum (II) (3b),[5-(2-aminorthyl)carbamoyl-2',3',5',-tri-O-acetyluridine] dichloroplatinum (II) (6b), [5-(2-aminoethyl)-carbamoyl]-2',3',5',-tri-O-acetyluridine] dichloroplatinum (II) (6b), [5-(2-aminoethyl)carbamoylu-ridine]dihloroplatinum (II) (7a), [6-(2-aminoethyl)carbamoyluridine]dichloroplatinum (II) (7b). These analogues were prepared from the key starting materials, 5-carboxyuracil (1a) and 6-carboxyuracil (1b) which were reacted with ethylenediamine to afford the respective N-(2-aminoethyl)uracil-5-carboxmide (2a) land N-(2-aminoethyl)uracil-6-carboxamide (2b). The cisplatin complexes 3a and 3b were obtained through the reaction of the respective 2a and 2b ficiently introduced on the .betha.-D-ribose ring via a Vorbruggen-type nucleoside coupling procedure with hexamethyldisilazane, trimethylchlorosilane and stannicchloride under anhydrous acetonitfile to yield the sterospecific .betha.-anomeric 5-carboxy-2',3',5'-tri-O-acetyluridine (4a) and 6-carboxy-2',3',5'-tri-O-acetyluridine (4b), respective 5-(2-aminoethyl)carbamoyl-2',3',5'-tri-O-acetyluridine (5a) and 6-(2-aminoethyl)carbamoyl-2',3',5'-tri-O-acetyluridine (5b). The diamino-uridines 5a and 5b were reacted with potassium tetrachloroplatinate (II) to give the novel nucleoside complexes, 6a and 6b respectively which were deacetylated into the free nucleosides, 7a and 7b by the treatment with CH/sub 3/ONa. The antitumor activities were evaluated against three cell lines (K-562, FM-3A and P-388).

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Performance Analysis of Fast Handover Scheme Based on Secure Smart Mobility in PMIPv6 Networks (프록시 모바일 IPv6 네트워크에서 안전한 스마트 이동성에 기반한 빠른 핸드오버 기법의 성능분석)

  • Yoon, KyoungWon;Jeong, Jongpil
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.13 no.5
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    • pp.121-133
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    • 2013
  • Defect-free transfer service on the Next-generation wireless network extensive roaming mobile node (MN) to provide efficient mobility management has become very important. MIPv6(Mobility IPv6) is one of mobility management scheme proposed by IETF(Internet Engineering Task Force), and IPv6-based mobility management techniques have been developed in various forms. One of each management techniques, IPv6-based mobility management techniques for PMIPv6 (MIPv6) system to improve the performance of a variety of F-PMIPv6 (Fast Handover for Proxy MIPv6) is proposed. However, the F-PMIPv6 is cannot be excellent than PMIPv6 in all scenarios. Therefor, to select a proper mobility management scheme between PMIPv6 and F-PMIPv6 becomes an interesting issue, for its potenrials in enhancing the capacity and scalability of the system. In this paper, we develop an analytical model to analyze the applicability of PMIPv6 and F-PMIPv6. Based on this model, we design an Secure Smart Mobility Support(SSM) scheme that selects the better alternative between PMIPv6 and F-PMIPv6 for a user according to its changing mobility and service characteristics. When F-PMIPv6 is adopted, SSM chooses the best mobility anchor point and regional size to optimize the system performance. Numerical results illustrate the impact of some key parameters on the applicability of PMIPv6 and F-PMIPv6. Finally, SSM has proven even better result than PMIPv6 and F-PMIPv6.

Extragalactic Sciences from SPICA/FPC-S

  • Jeong, Woong-Seob;Matsumoto, Toshio;Im, Myungshin;Lee, Hyung Mok;Lee, Jeong-Eun;Tsumura, Kohji;Tanaka, Masayuki;Shimonishi, Takashi;Lee, Dae-Hee;Pyo, Jeonghyun;Park, Sung-Joon;Moon, Bongkon;Park, Kwijong;Park, Youngsik;Han, Wonyong;Nam, Ukwon
    • The Bulletin of The Korean Astronomical Society
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    • v.38 no.1
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    • pp.36.2-36.2
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    • 2013
  • The SPICA (SPace Infrared Telescope for Cosmology & Astrophysics) project is a next-generation infrared space telescope optimized for mid- and far-infrared observation with a cryogenically cooled 3m-class telescope. The focal plane instruments onboard SPICA will enable us to resolve many astronomical key issues from the formation and evolution of galaxies to the planetary formation. The FPC-S (Focal Plane Camera - Sciecne) is a near-infrared instrument proposed by Korea as an international collaboration. Owing to the capability of both low-resolution imaging spectroscopy and wide-band imaging with a field of view of $5^{\prime}{\times}5^{\prime}$, it has large throughput as well as high sensitivity for diffuse light compared with JWST. In order to strengthen advantages of the FPC-S, we propose the studies of probing population III stars by the measurement of cosmic near-infrared background radiation and the star formation history at high redshift by the discoveries of active star-forming galaxies. In addition to the major scientific targets, to survey large area opens a new parameter space to investigate the deep Universe. The good survey capability in the parallel imaging mode allows us to study the rare, bright objects such as quasars, bright star-forming galaxies in the early Universe as a way to understand the formation of the first objects in the Universe, and ultra-cool brown dwarfs. Observations in the warm mission will give us a unique chance to detect high-z supernovae, ices in young stellar objects (YSOs) even with low mass, the $3.3{\mu}$ feature of shocked circumstance in supernova remnants. Here, we report the current status of SPICA/FPC project and its extragalactic sciences.

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Log-Structured B-Tree for NAND Flash Memory (NAND 플래시 메모리를 위한 로그 기반의 B-트리)

  • Kim, Bo-Kyeong;Joo, Young-Do;Lee, Dong-Ho
    • The KIPS Transactions:PartD
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    • v.15D no.6
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    • pp.755-766
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    • 2008
  • Recently, NAND flash memory is becoming into the spotlight as a next-generation storage device because of its small size, fast speed, low power consumption, and etc. compared to the hard disk. However, due to the distinct characteristics such as erase-before-write architecture, asymmetric operation speed and unit, disk-based systems and applications may result in severe performance degradation when directly implementing them on NAND flash memory. Especially when a B-tree is implemented on NAND flash memory, intensive overwrite operations may be caused by record inserting, deleting, and reorganizing. These may result in severe performance degradation. Although ${\mu}$-tree has been proposed in order to overcome this problem, it suffers from frequent node split and rapid increment of its height. In this paper, we propose Log-Structured B-Tree(LSB-Tree) where the corresponding log node to a leaf node is allocated for update operation and then the modified data in the log node is stored at only one write operation. LSB-tree reduces additional write operations by deferring the change of parent nodes. Also, it reduces the write operation by switching a log node to a new leaf node when inserting the data sequentially by the key order. Finally, we show that LSB-tree yields a better performance on NAND flash memory by comparing it to ${\mu}$-tree through various experiments.

A Study of the Establishment of Small and Medium Sized Architectural Design Firm BIM Environment based on Virtual Desktop Infrastructure (가상 데스크톱 인프라(VDI) 기술을 활용한 중소규모 설계사의 BIM 사용자 별 데스크탑 자원 할당 전략에 관한 연구)

  • Lee, Kyuhyup;Shin, Joonghwan;Kwon, Soonwook;Park, Jaewoo
    • Korean Journal of Construction Engineering and Management
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    • v.17 no.5
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    • pp.78-88
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    • 2016
  • Recently BIM technology has been expanded for using in construction project. However its spread has been delayed than the initial expectations, due to the high-cost of BIM infrastructure development, the lack of regulations, the lack of process and so forth. In design phase, especially, collaboration based on BIM system has being a key factor for successful next generation building project. Through the analysis of current research trend about IT technologies, virtualization and BIM service, data exchange such as drawing, 3D model, object data, properties using cloud computing and virtual server system is defined as a most successful solution. In various industrial fields, cloud computing technology is utilized as a promising solution which can reduce time and cost of hardware infrastructure. Among the cloud computing technology, VDI is receiving a great deal of attention from it market as an essential part cloud computing. VDI enables to host multiple individual virtual machines by using hypervisor. It has an advantage to easy main device management. Therefore, this study implements a step-by-step user's DaaS by analyzing the desktop resource data of the workers from Pre-design phase to Schematic design, Design develop and Construction design phase. It also develops BIM environment based on test of BIM modeler and designers in architectural design firm. The goal of the study is to enable the cloud computing BIM server. It provides cost saving, high-performance quality of working environment and cooperation's convenience and high security when doing BIM work in small and medium sized architectural design firm.

Development of Automated 3D Modeling System to Construct BIM for Railway Bridge (철도 교량의 BIM 구축을 위한 3차원 모델 생성 자동화 시스템 개발)

  • Lee, Heon-Min;Kim, Hyun-Seung;Lee, Il-Soo
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.31 no.5
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    • pp.267-274
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    • 2018
  • For successful BIM settlement, it is a key technic for engineer to design structures in the 3-dimensional digital space and to work out related design documents directly. Lately many BIM tool has been released and each supports their 3-dimensional object libraries. But it is not easy to apply those libraries to design transportation infra structures that were placed along the route(3-dimensional line). Moreover, in case of design changes, it is so difficult to reflect those changes with the integrated model that was assembled by them. Because of they were developed without consideration for redundancy of parameters between objects that were placed nearby or were related each other. In this paper, a method to develop module for modeling and placing 3-dimensional object for transportation infra structures is presented. The modules are employed by a parametric method and can deal with design changes. Also, for a railroad bridge, through developing user interface of the integrated 3-dimensional model that was assembled by those modules the applicability of them was reviewed.