• Title/Summary/Keyword: Karyotypes

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Comparison of electrophoretic karyotypes in fusarium

  • Min, Byung-Re
    • Journal of Microbiology
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    • v.33 no.4
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    • pp.334-338
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    • 1995
  • The electrophoretic karyotypes of 6 species in different Fusarium sections were examined by using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Intact chromosomal DNA was prepared from protoplasts and up to 9 distinct bands were separated on 0.7% or 0.8% agarose gel under several different conditions. Putative chromosome numbers varied from 6 to 9 amd polymorphic karyotypes were observed in different Fusarium sections. Using Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as standards, the sizes of the Fusarium spp. chromosomes were estimated. The electrophoretic karyotypes of F. moniliforme and F. subglutinans (section Liseola) were similar. Unidentified filamentour fungi, F. beomiforme was much closer to F. axysporum (section Elecgans) in karyotype and the karyotypes of F. napiforme were more similar to those of section Liseola than any other sections. F. graminearum (section Discolor) had a distinctive electrophoretic karyotype.

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Karyotypes of Korean Endemic Land Snail, Koreanohadra koreana (Gastropoda: Bradybaenidae)

  • Park, Gab-Man
    • The Korean Journal of Malacology
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    • v.27 no.2
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    • pp.87-90
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    • 2011
  • The karyotypes of Korean endemic land snail, Koreanohadra koreana, using air-drying method wereinvestigated. Somatic cells of this species had 2n = 58. Karyotypes were also analysed with 16 metacentric, 12 submetacentric and one subtelocentric chromosome pairs. Observed chromosomes ranged from 2.6 to $8.9{\mu}m$ and the total length was $122.3{\mu}m$. This is the second report on the chromosome numbers and the karyotype of K. koreana.

Variation of Univariate Flow Karyotypes and Chromosomal DNA Contents in Maize (Zea mays L.)

  • Lee, Jai-Heon;Lee, Myoung-Hoon;Kim, Kyung-Je
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.2
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    • pp.128-133
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    • 1998
  • Analyses of now karyotypes using different maize (Zea mays L.) inbred lines have been performed. The accumulation and isolation of high quality and quantity metaphase chromosomes from root tips can be achieved from many kinds of maize lines. The chromosome suspensions were prepared by a simple slicing method from synchronized maize root tips and analyzed with a now cytometry. The variations of experimental now karyotypes were detected among inbred lines in terms of the positions and/or the numbers of chromosome peaks. The 2C DNA amount among 8 inbred lines ranged from 5.09 to 5.52 pg. The variability of DNA content in maize chromosome 1 was 9.1 % ranging from 0.685 to 0.747 pg. The selection of appropriate maize lines is critical for sorting specific single chromosome types. At least five different chromosome types can be discriminated and sorted from five maize lines.

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Chromosome analysis by GTG banding technique in the DongGyeongi dogs (GTG banding에 의한 경주지방의 무미 또는 단미 형태의 개(경주개 동경이)의 핵형분석)

  • Choi, Seog-Kyu;Sung, Ki-Chang;Lee, Eun-Woo;Park, Chang-Eun
    • Korean Journal of Veterinary Service
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    • v.33 no.2
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    • pp.207-211
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    • 2010
  • None of the numerous published canis idiogram and karyotypes has yet been generally accepted as a standard one because the dog has 76 acrocentric autosomes of similar size and shape. The karyotypes of DongGyeongi dog were analysed by conventional trypsin/Giemsa staining (GTG-banding techniques), and were compared with one another. There were no variations in karyotypes which were analysed by conventional GTG-banding techniques, but differences were observed in G-banding patterns with sapsaree (or canis familiaris strains). It is not clear that these disagreements in G-banding patterns between strains of dog were caused by chromosome polymorphism or a difference in interpretation.

Giemsa C-banded Karyotypes and Their Relationship of Four Diploid Taxa in Allium (Allium속 이배체 4종의 C-분염 핵형 및 유연관계)

  • 서봉보
    • Journal of Plant Biology
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    • v.32 no.3
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    • pp.173-180
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    • 1989
  • Giemsa C-banded karyotypes of A. thunbergii, A. deltoide-fistulosum, A. cyaneum and A. cyaneum var. deltoides were analyzed, and the interspecific relationships were investigated on the basis of the C-banding patterns. The banding pattern of each species was unique and made possible an easy and clear separation among them. A. thunbergii and A. deltoide-fistulosum revealed very close banding relationship but a. cyaneum and A. cyaneum var. deltoides showed significant difference in banding pattern in spite of their close plant systematic position.

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Giemsa C-banded Karyotypes in Two Diploid and Two Tetraploid Allium Species (Allium속 이배체 2종과 사배체 2종의 Giemsa C-분염핵형)

  • 서봉보
    • Journal of Plant Biology
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    • v.32 no.3
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    • pp.181-188
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    • 1989
  • C-banded karyotypes in A. anisopodium, A. sacculiferum, A. deltoide-fistulosum and A. splendens were investigated. The chromosome compositions were diploid of 2n=16 in A anisopodium and A. splendens, and teiraploid of 2n=32 in a. sacculiferum and A. deltoide-fistulosum. The bands were distributed in telomeric parts of the chromosomes dominantly in addition to interstitial regions sporadically, resulting in the specific C-banded karyo types according to the species. No centromeric band was observed in these species except only one chromosome in A. deltoidefistulosum. The interspecific relationship based on the C-band distribution will be discussed.

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A Karvotvpic Studv on Six Korean Vespertilionid Bats (애기박쥐과에 속하는 한국산 박쥐류 6종에 대한 핵형학적 연구)

  • 유동호;윤명희
    • The Korean Journal of Zoology
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    • v.35 no.4
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    • pp.489-496
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    • 1992
  • The karyotypes of 4 Korean Myotis species (M. mystucinus gracilis, M. jormous tsuensis, M. duubentonii ussuriensis and M. macrodoctvlusp and 2 Pipistrelfus species (p. coreensis and p. abramusl belonging to the Vespertilionidae were examined. The 4 Mvotis species had the karyotypes of 2n : 44 with FN : 50 (M. m. gracilis and M. f. tsuensisl or 52 (M. d. ussuriensis and M. mncrodactylus). Furthermore the karyotype of R obrumus (2n : 26, FN : 441 seemed advanced compared with that of U coreensis (2n : 44, FN : 501 which is similar to the original karyotype of Mvotis species (2n : 44, FN : 50).

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Study on chromosomes survey of Korea native dogs (국내 토종개의 염색체 조사에 관한 연구)

  • Park, Chang-Eun
    • Korean Journal of Veterinary Service
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    • v.34 no.3
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    • pp.291-296
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    • 2011
  • The karyotype of the domestic dog is widely accepted as one of the difficult mammalian karyotypes to work. In contrast to many other animals, knowledge about the canine karyotype is quite sparse. The dog has a total of 78 chromosomes; all 76 autosomes are acrocentric in morphology and show only a gradual decrease in length. But appear to be quite small and difficult to identify unambiguously. To purchased standardization of chromosome in Korea native dog, there were analyzed by conventional trypsin/Giemsa staining (GTG-banding techniques), and were compared with 4, 6, 8, 11, 13, 17 chromosome. There were no variations in karyotypes which were analyzed by conventional GTG-banding techniques, but differences were observed in G-banding patterns with Sapsaree, Jindo, Gyeongju DongGyeong dogs, Welshi-Corgi. It is not clear that these disagreements in G-banding patterns between strains of dog were caused by chromosome polymorphism or a difference in interpretation. Comparative analysis of the distribution patterns of conserved segments defined by dog paints in the genomes of the Korea native dogs demonstrates that their differences in the karyotypes of these three species could have resulted from acrocentric banding patterns.

Isozymes and Karyotypes of Bufo Species (두꺼비 종류의 아이소자임과 염색체)

  • Yung J. Kim;Yang I, Sunwoo;Kang S. Rhee
    • The Korean Journal of Zoology
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    • v.19 no.3
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    • pp.123-138
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    • 1976
  • Isozymes and chromosomes of Bufo bufo and B. kangii were studied by starch gel electrohporesis and bone marrow air-drying method. Twently-three enzymes and nonenzymatic proteins in Bufo species collected in Korea provided a basis for the estimating the proportion of polymorphic loci in the genum. Of 23 loci controlling the proteins examined, 26% and 17$% were polymorphic in B. bufo and B. kangii, respectively. In B. bufo, 4 loci had 3 alleles and no loci had more than 2 alleles in B. kangii. Both species had same karyotypes with 22 chromosomes except one pair of chromosome which had sat-chromosomes in B. bufo karyotypes. The genetic similarity was approximately 0.5.

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Karyotypes of Two Species of Cultrinae (Cyprinidae) from Korea (한국산 강준치아과(Cultrinae) 2종의 핵형분석)

  • Song, Ho Bok;Park, Gab Man
    • Korean Journal of Ichthyology
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    • v.19 no.3
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    • pp.253-256
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    • 2007
  • The chromosome numbers of two species (Culter brevicauda and Erythroculter erythropterus) of Korean Cultrinae are investigated. In two species, the mitotic chromosomes from 24 groups with two chromosomes each indicated that it is a diploid. Their karyotypes and total length of chromosomes were slightly different from each other. The karyotype of C. brevicauda and E. erthropterus is 2n=48 (7M+10SM+7ST), FN=96 and 2n=48 (6M+10SM+8ST), FN=96. Observed chromosomes of C. brevicauda and E. erythropterus ranged from 5.4 to $2.2{\mu}m$ and 6.1 to $2.3{\mu}m$ in length, respectively.