• Title/Summary/Keyword: Kangwon National University

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Effects of Antioxidants on the Antioxidant and Apoptosis Genes Expression of IVM/IVF Hanwoo Embryos

  • Jang, H.Y.;Parkm C.K.;Cheong, H.T.;Kim, C.I.;Yim, S.V.;Kim, S.S.;Park, D.H.;Yang, B.K.
    • Proceedings of the KSAR Conference
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    • 2002.06a
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    • pp.69-69
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    • 2002
  • To investigate the effects of two antioxidants, aesculeitin and taurine, expression of apoptosis related genes and antioxidant enzyme gene in preimplantation Hanwoo embryos was determined by modified semi-quantitative single cell RT-PCR. Hanwoo embryos derived from in vitro maturation /in vitro fertilization were cultured in 5% CO₂ and 5% O₂ in CR₁aa medium at 37℃. Aesculeitin and taurine were added to medium at concentration of l㎍/㎖ and 2.5mM, respectively. (omitted)

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