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Comparative Transcriptome Analysis of the Response of Two Lines of Rapeseed (Brassica napus L.) to Cold Stress (유채 두 계통에서 저온 스트레스에 반응하는 전사체 발현 비교 분석)

  • Lee, Ji-Eun;Kim, Kwang-Soo;Cha, Young-Lok;An, Da-Hee;Byun, Jong-Won;Kang, Yong-Ku
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.1
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    • pp.37-71
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    • 2021
  • Rapeseed is a typical winter crop, and its freezing stress tolerance is a major feature for winter survival. Therefore, it is important to comprehend clearly the physical and molecular mechanisms of rapeseed under freezing stress conditions. This study investigates the physical and transcriptome changes of two rapeseed lines, 'J8634-B-30' and 'EMS26', under cold acclimation and freezing temperature treatments. The proline content of 'J8634-B-30' at 5 ℃ increased 8.7-fold compared to that before treatment, and there was no significant change in that of 'EMS26' RNA-sequencing analysis revealed 5,083 differentially expressed genes (DEGs) of 'J8634-B-30' under cold acclimation condition. Among the genes, 2,784 (54.8%) were up-regulated and 2,299 (45.2%) were down-regulated. The DEGs of 'EMS26' under cold acclimation condition were 5,831 genes, and contained 2,199 up-regulated genes (37.7%) and 3,632 down-regulated genes (62.3%). Among them, only DEGs annotated in the cold response-related signaling pathways were selected, and their expression in the two rapeseed lines was compared. Comparative DEGs analysis indicated that cold response related signaling pathways are proline metabolism and ABA (Abscisic acid) signaling. And ICE (Inducer of CBF expression) - CBF (C-repeat-binding factor) - COR (Cold-regulated) signaling were the significantly differentially expressed transcripts in the two rapeseed lines. The major induced transcripts of 'J8634-B-30' induced P5CS (Δ'-pyrroline-5-carboxylate synthetase), which is related to proline biosynthesis, PYL (pyrabactin resistance-like protein, ABA receptor) and COR413 (cold-regulated 413 plasma membrane 1). In conclusion, these result provide a foundation for understanding the mechanisms of freezing stress tolerance in rapeseeds. Further functional studies should be performed on the freezing stress-related genes identified in this study, which can contribute to the transgenic and molecular breeding for freezing stress tolerance in rapeseed.

Carcass Characteristics and Primal Cut Yields of Hanwoo as Affected by Sex (한우의 성별에 따른 도체특성과 부분육 생산수율 조사)

  • Seo, Hyun-Woo;Ba, Hoa Van;Kim, Yoon-Seok;Kang, Sun-Moon;Seol, Kuk-Hwan;Seong, Pil-Nam;Moon, Sung-Sil;Kim, Jin-Hyoung;Cho, Soo-Hyun
    • Journal of agriculture & life science
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    • v.53 no.4
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    • pp.77-92
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    • 2019
  • The purpose of this study was to investigate the carcass characteristics and the primal cut yields from carcasses according to the sex of Hanwoo. Experimental animals comprised of 80 cows, 29 bulls, and 71 steers. After slaughtering, they were evaluated to estimate the productivity of carcass components by market weight, and to predict the beef carcass yield. The average of fasting weight of cow, bull and steer were 634.75 kg, 721.86 kg, and 754.10 kg respectively. Market weight of Hanwoo at cold carcass weight, primal lean cut weight, sub-primal lean cut weight, bone weight and fat weight were 381.01~467.60 kg, 240.79~310.36 kg, 208.27~276.47 kg, 57.23~76.28 kg and 89.19~138.97 kg respectively. Body measurement traits had side length, hindquarter length, cervical vertebrae length, lumbar vertebrae length, sacral vertebrae length, 6th lumbar vertebrae~heel length, 5~6th thoracic vertebrae breadth, 4~5th lumbar vertebrae breadth, 5th sacral vertebrae breadth, 7~8th thoracic vertebrae girth and 7~8th thoracic vertebrae thick were highest length in steer. Carcass weight (CW) were significantly (p<0.05) affected by sex and live weight. The lean meat percentage, fat percentage and bone percentage based on the weight of cold carcasses were significantly different (p<0.05) between sex groups. The primal cuts rate for deungsim, moksim, abdari, udun, suldo, yangjee and satae were higher in bulls than those of the caws and steers. The carcass by-product weight rate in steer had lower oxtail and rib cartilage than cow and bull, while cow showed lower beef leg bones and jappyeo than bull steer (p<0.05). Body measurement traits was always important for primal cut weight estimates.

Community Structure and Health Assessment of Macrobenthos in Tidal Flats along the West Coast of Korea in Spring and Summer (서해안 갯벌의 춘·하계 대형저서동물의 군집구조 및 건강도 평가)

  • Ong, Giho;Jeon, Seung Ryul;Koo, Jun Ho;Park, Jong-Woo;Jeung, Hee-Do;Kang, Jung-Ha;Cho, Yoon-Sik
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.27 no.4
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    • pp.500-509
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    • 2021
  • This study investigated the characteristics of a macrobenthos community and sediment environment and assessed the health of tidal flats along the west coast of Korea. A survey was conducted from Ganghwa-do to Mokpo, Jeollanam-do in April (spring) and August (summer) 2017, and April (spring) 2018. The sediment grain sizes in the Chungcheongnam-do region were coarser, and the sediment in the Gyeonggi-do·Incheon, Jeollanam-do, and Jeollabuk-do regions were finer. A total of 140 macrobenthic species were collected from this study and using a cluster similarity analysis of the macrobenthos community, they were divided into four groups. Group2 was associated with Manila clam farm stations, and Ruditapes phillipinarum, Nephtys polybranchia and Lumbrineris nipponica were dominant. Group4 included some sites with finer sediment composed relatively, and Eteone longa and Nemertea unid. appeared at a high frequency. From the health assessment of the western tidal flat, the ISEP and BHI indices had a "High status," and the AMBI index had a "Good status." In conclusion, the tidal flats along the west coast of Korea have good ecological health. However, pollution indicator species such as Theora lata and Capitella capitata have appeared in some areas. Therefore, periodic administration and interventions are necessary to prevent deterioration of the tidal flat environment.

Development of Estimation Equation for Minimum and Maximum DBH Using National Forest Inventory (국가산림자원조사 자료를 이용한 최저·최고 흉고직경 추정식 개발)

  • Kang, Jin-Taek;Yim, Jong-Su;Lee, Sun-Jeoung;Moon, Ga-Hyun;Ko, Chi-Ung
    • Journal of agriculture & life science
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    • v.53 no.6
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    • pp.23-33
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    • 2019
  • In accordance with a change in the management information system containing the management record and planning for the entire national forest in South Korea by an amendment of the relevant law (The national forest management planning and methods, Korea Forest Service), in this study, average, the maximum, and the minimum values for DBH were presented while only average values were required before the amendment. In this regard, there is a need for an estimation algorithm by which all the existing values for DBH established before the revision can be converted to the highest and the lowest ones. The purpose of this study is to develop an estimation equation to automatically show the minimum and the maximum values for DBH for 12 main tree species from the data in the national forest management information system. In order to develop the estimation equation for the minimum and the maximum values for DBH, there was exploited the 6,858 fixed sample plots of the fifth and the sixth national forest inventory between in 2006 and 2015. Two estimation models were applied for DBH-tree age and DHB-tree height using such growth variables as DBH, tree age, and height, to draw the estimation equation for the maximum and the minimum values for DBH. The findings showed that the most suitable model to estimate the minimum and the maximum values for DBH was Dmin=a+bD+cH, Dmax=a+bD+cH with the variables of DBH and height. Based on these optimal models, the estimation equation was devised for the minimum and the maximum values for DBH for the 12 main tree species.

Effect of Concentration of Nutrient Solution on Water and Nutrient Uptake of Tomato Cultivars in Hydroponics (배양액 농도가 수경재배 토마토의 품종별 생육과 양수분 흡수특성에 미치는 영향)

  • Choi, Gyeong Lee;Yeo, Kyung Hwan;Choi, Su Hyun;Jeong, Ho Jeong;Kang, Nam Jun
    • Journal of agriculture & life science
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    • v.53 no.1
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    • pp.13-21
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    • 2019
  • This study was carried out to acquire basic data for a long-term hydroponic culture through investigating water and inorganic ion uptake characteristics at different EC level of nutrient solution of three tomato varieties. Three different tomato varieties, the European type(cv. Daphnis), the Asian type(cv. Super Doterang) and cherry type(cv. Minichal), were used for the investigation. Also, the deep flow technique(DFT) was applied. The three different electrical conductivity(EC) level(1.0, 2.0, 3.0, and 4.0 dS·m-1) of hydroponic nutrient solution were used as variable. At a high EC level of nutrient solution, the leaf area and fresh weight decreased in the early stage, and its growth(plant height, leaf number, leaf area, fresh-weight) was poor with salt stress. Result showed that the higher the EC level of the nutrient solution, the lesser was water uptake. The water uptake was not significantly different from varieties in the first survey, but In the second survey, the 'Daphnis' did not show a significant decrease in water uptake in the EC level higher than 2.0 dS·m-1., on the other hand, 'Super Doterang' presented very low water uptake. At a low EC level, N, P, and K, were absorbed more than the concentration of the irrigation water, while Ca, Mg, S uptake were low. At a high EC level, almost ions absorbed less than 50% of the initial concentration of irrigation water. Thus, imbalance among ions was severe at low EC level compared to high EC level. 'Daphnis' was a variety that effectively utilize nutrients under nutrient stress, showing high absorption at low concentration condition and low absorption at high concentration condition. However, 'Daphnis' suffered most seriously by absorbing nutrients excessively.

A Comparison Study of Cost Components to Estimate the Economic Loss from Foodborne Disease in Foreign Countries (국외 식중독으로 인한 손실비용 추정을 위한 항목 비교 연구)

  • Hyun, Jeong-Eun;Jin, Hyun Joung;Kim, Yesol;Ju, Hyo Jung;Kang, Woo In;Lee, Sun-Young
    • Journal of Food Hygiene and Safety
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    • v.36 no.1
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    • pp.68-76
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    • 2021
  • Foodborne outbreaks frequently occur worldwide and result in huge economic losses. It is the therefore important to estimate the costs associated with foodborne diseases to minimize the economic damage. At the same time, it is difficult to accurately estimate the economic loss from foodborne disease due to a wide variety of cost components. In Korea, there are a limited number of analytical studies attempting to estimate such costs. In this study we investigated the components of economic cost used in foreign countries to better estimate the cost of foodborne disease in Korea. Seven recent studies investigated the cost components used to estimate the cost of foodborne disease in humans. This study categorized the economic loss into four types of cost: direct costs, indirect costs, food business costs, and government administration costs. The healthcare costs most often included were medical (outpatient) and hospital costs (inpatient). However, these cost components should be selected according to the systems and budgets of medical services by country. For non-healthcare costs, several other studies considered transportation costs to the hospital as an exception to the cost of inpatient care. So, further discussion is needed on whether to consider inpatient care costs. Among the indirect costs, premature mortality, lost productivity, lost leisure time, and lost quality of life/pain, grief and suffering costs were considered, but the opportunity costs for hospital visits were not considered in any of the above studies. As with healthcare costs, government administration costs should also be considered appropriate cost components due to the difference in government budget systems, for example. Our findings will provide fundamental information for economic analysis associated with foodborne diseases to improve food safety policy in Korea.

Effects of Light Intensity and Electrical Conductivity Level on Photosynthesis, Growth and Functional Material Contents of Lactuca indica L. 'Sunhyang' in Hydroponics (수경재배에서 광도와 양액 농도가 베이비 산채 왕고들빼기 '선향' 광합성과 생육 및 기능성 물질 함량에 미치는 영향)

  • Kim, Jae Kyung;Jang, Dong Cheol;Kang, Ho Min;Nam, Ki Jung;Lee, Mun Haeng;Na, Jong Kuk;Choi, Ki Young
    • Journal of Bio-Environment Control
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    • v.30 no.1
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    • pp.1-9
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    • 2021
  • This study was conducted to examine the changes of photosynthesis, growth, chlorophyll contents and functional material contents in light intensity and EC concentration of wild baby leaf vegetable, Indian lettuce (Lactuca indica L. cv. 'Sunhyang') in DFT hydroponics. The cultivation environment was 25±1℃ of temperature and 60±5% of relative humidity in growth system. At 14 days after sowing, combination effect of light intensity (Photosynthetic Photon Flux Density (PPFD 100, 250, 500 µmol·m-2·s-1) and EC level (EC 0.8, 1.4, 2.0 dS·m-1) of nutrient solution was determined at the baby leaf stage. The photosynthesis rate, stomatal conductance, transpiration rate and water use efficiency of Indian lettuce increased as the light intensity increased. The photosynthesis rate and water use efficiency were highest in PPFD 500-EC 1.4 and PPFD 500-EC 2.0 treatment. The chlorophyll content decreased as the light intensity increased, but chlorophyll a/b ratio increased. Leaf water content and specific leaf area decreased as light intensity increased and a negative correlation (p < 0.001) was recognized. Plant height was the longest in PPFD 100-EC 0.8 and leaf number, fresh weight and dry weight were the highest in PPFD 500-EC 2.0. Anthocyanin and total phenolic compounds were the highest in PPFD 500-EC 1.4 and 2.0 treatment, and antioxidant scavenging ability (DPPH) was high in PPFD 250 and 500 treatments. Considering the growth and functional material contents, the proper light intensity and EC level for hydroponic cultivation of Indian lettuce is PPFD 500-EC 2.0, and PPFD 100 and 250, which are low light conditions, EC 0.8 is suitable for growth.

Effect of Irrigation Starting Point of Soil on Chlorophyll Fluorescence, Stem Sap Flux Relative Rate and Leaf Temperature of Cucumber in Greenhouse (시설 토양 오이재배에서 관수개시점 처리가 광합성 형광반응, 줄기수액흐름 및 엽온에 미치는 영향)

  • An, Jin Hee;Jeon, Sang Ho;Choi, Eun Yong;Kang, Ho Min;Na, Jong Kuk;Choi, Ki Young
    • Journal of Bio-Environment Control
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    • v.30 no.1
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    • pp.46-55
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    • 2021
  • This experiment was conducted to investigate the effect on chlorophyll fluorescence, stem sap flux relative rate (SFRR) and leaf temperature of cucumber when irrigation is controlled using a soil moisture tensiometer. Cucumber (Cucumis sativus L.) 'Chungchun' was irrigated of 10-10-20 kPa and 20-10-10 kPa by soil starting point of irrigation at each growth stage. At the 66 days after treatment (DAT) of 736 to 854 W·m-2 and above 32℃, chlorophyll fluorescence variables (Fo, Fm, Fv/Fm) values showed significantly different between treatments. The Fo and Fv/Fm value in the daytime (10:30 am to 6:00 pm) at 66 DAT was higher in 20-10-10 kPa treatment than in 10-10-20 kPa treatment. The Fv/Fm value decreased when the leaf temperature was increased. There was no difference in leaf growth (length, width and area) at 28 and 66 DAT, but the chlorophyll content (SPAD value) was significantly higher in 20-10-10kPa treatment. SFRR and leaf temperature increased with light intensity and temperature increased. In both treatments, the SFRR started to increase sharply between 8 am and 9 am when the solar radiation is 170 W·m-2 or higher. The soil temperature of the treatments decreased after irrigation, that showed 31.0℃ at 10-10-20kPa and 28.5℃ at 20-10-10kPa on July 5 (820W·m-2 at 1 pm). However, there was no difference in SFRR, leaf temperature, temperature difference (leaf temperature - air temperature) and VPD between treatments. SFRR was significantly positive correlate with the leaf temperature (p < 0.01, r = 0.770). The SFRR and leaf temperature showed positive significant correlation with solar radiation, temperature, soil temperature, soil moisture content and VPD. There was a negative significant correlation with relative humidity and temperature difference.

Relationship between Earthquake and Fluctuation of Water Level in Active Fault Zone and National Groundwater Monitoring Wells of Gyeongju Area (경주 활성단층대 및 주변 국가지하수 관측정에서 지진과 수위변동 상관관계 연구)

  • Jang, Hyeon-Woo;Jeong, Chan-Ho;Lee, Yong-Cheon;Lee, Yu-Jin;Hong, Jin-Woo;Kim, Cheon-Hwan;Kim, Young-Seog;Kang, Tae-Seob
    • The Journal of Engineering Geology
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    • v.30 no.4
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    • pp.617-634
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    • 2020
  • The purpose of this study is to investigate the relationship of between earthquakes and fluctuation of water level in a groundwater well of the active-fault zone and 124 national groundwater monitoring wells in Gyeongju area. The spatial and temporal relationships between the fluctuation of water level and the earthquake were analyzed by the calculation of earthquake effectiveness (ε) and q-factor which are the function of earthquake magnitude and distance from epicenter. Two earthquake events of E1 (April 22, 2019, M 3.8) and E2 (June 11, 2019, M 2.5) show a close relationship with a post-seismic 83 cm decrease and a pre-seismic 76 cm increase in water level at the active fault zone of Dangu-ri, respectively. The spatial analysis of water level fluctuation data in National Groundwater Monitoring Networks caused by earthquake events shows a more distinct response in deep groundwater around fault zones than other area, and a greater change in deep groundwater than shallow groundwater. It's inferred that the decrease and increase in groundwater level are affected by the expansion of fractures and compression of rock mass due to seismic stress, respectively. The effective ranges of ε-value and q-factor of the monitoring well in Dangu-ri were calculated as 2.70E-10~5.60E-10 and 14.4~18.0, respectively.

Standardization and Management of Interface Terminology regarding Chief Complaints, Diagnoses and Procedures for Electronic Medical Records: Experiences of a Four-hospital Consortium (전자의무기록 표준화 용어 관리 프로세스 정립)

  • Kang, Jae-Eun;Kim, Kidong;Lee, Young-Ae;Yoo, Sooyoung;Lee, Ho Young;Hong, Kyung Lan;Hwang, Woo Yeon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.3
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    • pp.679-687
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    • 2021
  • The purpose of the present study was to document the standardization and management process of interface terminology regarding the chief complaints, diagnoses, and procedures, including surgery in a four-hospital consortium. The process was proposed, discussed, modified, and finalized in 2016 by the Terminology Standardization Committee (TSC), consisting of personnel from four hospitals. A request regarding interface terminology was classified into one of four categories: 1) registration of a new term, 2) revision, 3) deleting an old term and registering a new term, and 4) deletion. A request was processed in the following order: 1) collecting testimonies from related departments and 2) voting by the TSC. At least five out of the seven possible members of the voting pool need to approve of it. Mapping to the reference terminology was performed by three independent medical information managers. All processes were performed online, and the voting and mapping results were collected automatically. This process made the decision-making process clear and fast. In addition, this made users receptive to the decision of the TSC. In the 16 months after the process was adopted, there were 126 new terms registered, 131 revisions, 40 deletions of an old term and the registration of a new term, and 1235 deletions.