• Journal of Microbiology and Biotechnology
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• v.26 no.4
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• pp.700-709
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• 2016

The mitogen-activated protein kinase HOG1 (high-osmolarity glycerol response pathway) plays a crucial role in the response of yeast to hyperosmotic shock. Trichosporonoides oedocephalis produces large amounts of polyols (e.g., erythritol and glycerol) in a culture medium. However, the effects of HOG1 gene knockout and environmental stress on the production of these polyols have not yet been studied. In this study, a To-HOG1 null mutation was constructed in T. oedocephalis using the loxP-Kan-loxP/Cre system as replacement of the targeted genes, and the resultant mutants showed much smaller colonies than the wild-type controls. Interestingly, compared with the wild-type strains, the results of shake-flask culture showed that To-HOG1 null mutation increased erythritol production by 1.44-fold while decreasing glycerol production by 71.23%. In addition, this study investigated the effects of citric acid stress on the T. oedocephalis HOG1 null mutants and the wild-type strain. When the supplementation of citric acid in the fermentation medium was controlled at 0.3% (w/v), the concentration of erythritol produced from the wild-type and To-HOG1 knockout mutant strains improved by 18.21% and 21.65%, respectively.

• Journal of the Korean Institute of Rural Architecture
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• v.15 no.2
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• pp.25-32
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• 2013

The purpose of this study is to analyze the residential space composition and extension of Jeonja-jip and Dureong-jip in Samcheok region. The results of this study are as follows. It appears three residential plan types - Jeonja-jip with Anmaru(a room made of wooden floor), Jeonja-jip without Anmaru and Dureong-jip with Anmaru- in survey region. It was needed the extension of residential space at Jeonja-jip(a house type with two-row rooms which be composed of 田type under one roof) and Dureong-jip(a house type with inner floor which be surrounded by rooms under one roof) because of shortage of sleeping space, working space and keeping space owing to growing large family. The solutions of these shortages will be the extension of space in houses. Jeonja-jip's basic type which is the Kyup-Jip(a house type with two-row rooms under one roof) of 一 type with 6 Kans(Kan, a unit to count room to divided with four posts) is transformed and extended to 田shaped house with 11 Kans toward X-axis orientation. Dureong-Jip's basic type which is ㄱ type with 7 Kans is transformed and extended to the Sekyup-Jip with 9 Kans and the Nekyup-Jip(a house type with four-row rooms under one roof) with 12 Kans toward Y-axis orientation by insertion and addition. Jeonja-jip was developed into Kyup-Jip with front 4 or 5 Kans and side 2 Kans instead of being done into Sekyup-jip or Nekyup-Jip with insufficient day lighting and ventilation problems. Jeonja-jip and Dureong-jip were stronger than any other traditional houses in tendency to reserve Magu(cowshed) and Chikkan(toilet) in a house.

• Journal of Astronomy and Space Sciences
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• v.29 no.3
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• pp.259-267
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• 2012

Polar cap potential has long been considered as an indicator for the amount of energy flowing in the magnetosphere-ionosphere system. Thus, the estimation of polar cap potential is important to understand the physical process of the magnetosphere. To estimate the polar cap potential in the Northern Hemisphere, merging electric field by Kan & Lee (1979) is adopted. Relationships between the PC index and calculated merging electric field ($E^*$) are examined during full-time and storm-time periods separately. For this purpose Dst, AL, and PC indices and solar wind data are utilized during the period from 1996-2003. From this linear relationship, polar cap potential (${\Phi}^*$) is estimated using the formula by Doyle & Burke (1983). The values are represented as $58.1{\pm}26.9$ kV for the full-time period and $123.7{\pm}84.1$ kV for a storm-time period separately. Considering that the average value of polar cap potential of Doyle & Burke (1983) is about 47 kV during moderately quiet intervals with the S3-2 measurements, these results are similar to such. The monthly averaged variation of Dst, AL, and PC indices are then compared. The Dst and AL indices show distinct characteristics with peaks during equinoctial season whereas the average PC index according to the month shows higher values in autumn than in spring. The monthly variations of the linear correlation coefficients between solar wind parameters and geomagnetic indices are also examined. The PC-AL linear correlation coefficient is highest, being 0.82 with peaks during the equinoctial season. As with the AL index, the PC index may also prove useful for predicting the intensity of an auroral substorm. Generally, the linear correlation coefficients are shown low in summer due to conductance differences and other factors. To assess the role of the PC index during the recovery phase of a storm, the relation between the cumulative PC index and the duration is examined. Although the correlation coefficient lowers with the storm size, it is clear that the average correlation coefficient is high. There is a tendency that duration of the recovery phase is longer as the PC index increases.

• Proceedings of the KSRS Conference
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• v.1
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• pp.11-14
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• 2006

We present an overview on the observation and research for the China seas using both field experiments and multi-sensor satellite data at ORSI/OUC, covering two topics: (1) Spatial and temporal distribution of internal waves in the China Seas and retrieval of internal wave parameters; (2) Retrieval, validation, and cross-comparison of multi-sensor ocean color data as well as ocean optics in situ experiments in the East China Sea. We also present an incoherent Doppler wind lidar, developed by ORSI, and its observation for marine-atmospheric boundary layer.

• Journal of Ginseng Research
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• v.25 no.1
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• pp.41-44
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• 2001

This study aimed to identify the flowering and fruiting characteristics of 2 varieties (Chunpoong and Yunpoong), 3 superior lines (KG103, KG104, KG105) and Hwangsookjong selected for development of varieties of Panax ginseng C.A. Meyer. The flowering date of Yunpoong and KG103 line showed earlier than any other lines or variety. Fertility ratio of KG104 was higher than others and the lowest with KG103 showing over 60% in all varieties and lines tested. On the fruiting type of lines, ratio of single ovary type of Chunpoong and KG105 was higher than double ovary type while Yunpoong, KG104 and Hwangsookjong were formed more double ovary type. Seed yield of KG104 per stem was the highest but Yunpoong showed the highest per kan (90 ${\times}$ 180 cm). Seed size of KG103 was the biggest among the lines tested, meanwhile that of Chunpoong and Hwangsookjong were smaller.

• Radiation Oncology Journal
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• v.23 no.3
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• pp.194-200
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• 2005

An ameloblastic carcinoma is a very rare odontogenic malignant tumor, which sometimes have a benign clinical nature, but typically have aggressive features, with large ulcerative lesion and extensive local destruction. These clinical characteristics make the complete surgical removal of a tumor difficult. As a consequence, a poor prognosis may result due to local recurrence and distant metastasis. For this reason, it is important to consider adjuvant therapies for high-risk ameloblastic carcinoma patients. Here, the case of a recurrent ameloblastic carcinoma that occurred in the mandible after primary surgery is reported. The lesion was treated with further local excision, followed by postoperative radiotherapy. Also, a few remarks on the role of postoperative radiotherapy in patient with ameloblastic carcinoma have been made from a review of the current literatures on the treatment of this type of lesion.

• Radiation Oncology Journal
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• v.31 no.4
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• pp.216-221
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• 2013

Purpose: The purpose of this retrospective study was to evaluate the efficacy and feasibility of short-course hypofractionated radiotherapy (RT) for the palliation of uterine cervical cancer. Materials and Methods: Seventeen patients with cancer of the uterine cervix, who underwent palliative hypofractionated 3-dimensional conformal radiotherapy between January 2002 and June 2012, were retrospectively analyzed. RT was delivered to symptomatic lesions (both the primary mass and/or metastatic regional lymph nodes). The total dose was 20 to 25 Gy (median, 25 Gy) in 5 Gy daily fractions. Results: The median follow-up duration was 12.2 months (range, 4 to 24 months). The median survival time was 7.8 months (range, 4 to 24 months). Vaginal bleeding was the most common presenting symptom followed by pelvic pain (9 patients). The overall response rates were 93.8% and 66.7% for vaginal bleeding control and pelvic pain, respectively. Nine patients did not have any acute side effects and 7 patients showed minor gastrointestinal toxicity. Only 1 patient had grade 3 diarrhea 1 week after completion of treatment, which was successfully treated conservatively. Late complications occurred in 4 patients; however, none of these were of grade 3 or higher severity. Conclusion: Short-course hypofractionated RT was effective and well tolerated as palliative treatment for uterine cervical cancer.

• Korean Journal of Microbiology
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• v.48 no.2
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• pp.171-174
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• 2012

THOC1/Hpr1 is one subunit of THO complex that is an evolutionally conserved assembly involved in the mRNP packaging and mRNA export during transcription elongation. In fission yeast Schizosaccharomyces pombe, an ortholog (spHpr1) of THOC1/Hpr1 was identified based on sequence alignment. A deletion mutant in a diploid strain was constructed by replacing one of spHpr1-coding region with a $kan^r$ gene using one-step gene disruption method. Tetrad analysis showed that the sphpr1 is essential for growth. Over-expression of sphpr1 from strong nmt1 promoter caused no defects of growth and mRNA export. However, repression of the sphpr1 expression resulted in growth inhibition accompanied by accumulation of poly$(A)^+$ RNA in the nucleus. These results suggest that spHpr1 is involved in mRNA export from the nucleus to cytoplasm.

• Journal of Advanced Marine Engineering and Technology
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• v.32 no.1
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• pp.33-41
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• 2008

Bearing damages by shaft misalignment have frequently been happened in marine ships. Specially. after stern tube bearing damage and failure for large crude oil carriers have been reported several times. However. the bearing reaction of the after stern tube bearing cannot be measured by jack-up test due to the hull structure condition. Therefore, when the jack-up test is used for the bearing reaction measurements, the bearing reaction for the after stern tube bearing obtained from the theoretical calculation method have to be used. In this paper, the shaft alignment on the large oil crude carrier is theoretically calculated and the differences between the calculated and actual installed bearing reaction values are compared. The bearing reactions for forward stern tube bearing and intermediate bearing are calculated by the simple formula using the strain gauge bending moments obtained from the measurements. Their reliability is confirmed by comparing the bearing reactions from jack-up test and the bearing reaction for after stern tube bearing is calculated by the same test. Also, the bearing reactions on the after stern tube bearing, forward stern tube bearing and intermediate shaft bearing under all operating conditions are calculated by using the bending moments obtained from the measurements and it is confirmed that the differences of the bearing reaction for all operating conditions are caused from hull deflection. The results of this study should prove useful for the future projects of the alignment calculation including the hull deflection effectiveness.

• Molecules and Cells
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• v.38 no.3
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• pp.243-250
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• 2015

Patterning of the polar axis during the early leaf developmental stage is established by cell-to-cell communication between the shoot apical meristem (SAM) and the leaf primordia. In a previous study, we showed that the DRL1 gene, which encodes a homolog of the Elongator-associated protein KTI12 of yeast, acts as a positive regulator of adaxial leaf patterning and shoot meristem activity. To determine the evolutionally conserved functions of DRL1, we performed a comparison of the deduced amino acid sequence of DRL1 and its yeast homolog, KTI12, and found that while overall homology was low, well-conserved domains were presented. DRL1 contained two conserved plant-specific domains. Expression of the DRL1 gene in a yeast KTI12-deficient yeast mutant suppressed the growth retardation phenotype, but did not rescue the caffeine sensitivity, indicating that the role of Arabidopsis Elongator-associated protein is partially conserved with yeast KTI12, but may have changed between yeast and plants in response to caffeine during the course of evolution. In addition, elevated expression of DRL1 gene triggered zymocin sensitivity, while overexpression of KTI12 maintained zymocin resistance, indicating that the function of Arabidopsis DRL1 may not overlap with yeast KTI12 with regards to toxin sensitivity. In this study, expression analysis showed that class-I KNOX genes were downregulated in the shoot apex, and that YAB and KAN were upregulated in leaves of the Arabidopsis drl1- 101 mutant. Our results provide insight into the communication network between the SAM and leaf primordia required for the establishment of leaf polarity by mediating histone acetylation or through other mechanisms.