• 한국실내디자인학회논문집
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• 제14권3호
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• pp.84-94
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• 2005

The purpose of this study is to establish the identity of the traditional residential space in Korea and Japan as analyzing the spatial characteristics and sociocultural roles of the 'in-between space' shown in their traditional dwelling space. Although they share culture area, it is meaningful to clarify the identity of both countries with many differences as the point of localization In globalization and the point of presenting the current and future directions of residential culture in both countries. Records and field studies have been carried out for the spatial characteristics and the sociocultural role focused on Ma-dang and Dae-chung in case of the 'in-between space' in Korean traditional dwellings and Inner housing, Doma, and Engawa in Japan. According to these findings, the spatial characteristics in common between Korea and Japan are open, half-open, separation, mitigation, integration, variation, continuation, hierarchy, mediation, and expansion. The 'in-between space', built by the separate Chae and Kan, of Korean traditional dwellings shows the separation by closed walls and the exclusive use by the fixed walls. In case of Japan, the integrated in-between space built by the separation of Kan shows open and connective tendencies and the variableness of walls has the tendency making ambiguous space with spatial softness. The sociocultural role of the 'in-between space' in Korean traditional dwelling space functions as the mitigation between the interior and exterior of the house as well as the male and female. Because the conception for protecting the private life is too weak, the sociocultural role of the 'in-between space' in Japanese traditional dwelling space seldom forms the space for an individual or the specific person among family. The space to welcome a guest functions as the mitigation between residents and outsiders as it is located in the inside of the private dwelling space.

• 건축역사연구
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• 제18권3호
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• pp.7-25
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• 2009

Hakbongjongtaek(The head house of Uisong Kim family, 鶴峯宗宅), firstly built in the early 17th century, was moved to its neighboring place in the late 18th century. The man who led the relocation(移建) of the house was the eldest grandson of the family, Jong-soo, Kim. He made and overall plan and also participated in building the house. There remain four Gados(family paintings, 가도(家圖)) related to the relocation plan. This paper aims to make an analysis of these Gados, and through analysis, to find what was the essential poing of the relocation plan and how the point was represented in the paintings. The result of analysis is as follows: 1. The main focus of relocation plan was the form of Anchae(the inner house). Anchae was designed as asymmetrical form, and restricted to four kan. Only Andaechung needed to be 6 kan size for religious ceremonies. 2. For the design of Sarangchae, the displacement of large Sarangbang and small Sarangbang was an important issue. There were two ways of layout: parallel type and facing type. The latter was chosen. 3. The representation and techniques of Gado is quite concrete, in spite of differences among them. The expression of doors, windows, attic and kitchen was based on the understanding of space. Also the spatial division, which was expressed line on the grid, was based on the scaled ruler. As we've seen before, painting the relocation plan was a kind of endeavors to make the housing type as a realization of Garye. Also, we can find out that role of the eldest grandson of the family was quite important to carry out the plan. As well as, it was meaningful to examine Sadaebu (the aristocrat of Chosun)'s perception of housing.

• Journal of Microbiology and Biotechnology
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• 제32권11호
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• pp.1471-1478
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• 2022

2'-Fucosyllactose (2'-FL), the most abundant fucosylated oligosaccharide in human milk, has multiple beneficial effects on human health. However, its biosynthesis by metabolically engineered Escherichia coli is often hampered owing to the insolubility and instability of α-1,2-fucosyltransferase (the rate-limiting enzyme). In this study, we aimed to enhance 2'-FL production by increasing the expression of soluble α-1,2-fucosyltransferase from Helicobacter pylori (FucT2). Because structural information regarding FucT2 has not been unveiled, we decided to improve the expression of soluble FucT2 in E. coli via directed evolution using a protein solubility biosensor that links protein solubility to antimicrobial resistance. For such a system to be viable, the activity of kanamycin resistance protein (Kan^R) should be dependent on FucT2 solubility. Kan^R was fused to the C-terminus of mutant libraries of FucT2, which were generated using a combination of error-prone PCR and DNA shuffling. Notably, one round of the directed evolution process, which consisted of mutant library generation and selection based on kanamycin resistance, resulted in a significant increase in the expression level of soluble FucT2. As a result, a batch fermentation with the ΔL M15 pBCGW strain, expressing the FucT2 mutant (F#1-5) isolated from the first round of the directed evolution process, resulted in the production of 0.31 g/l 2'-FL with a yield of 0.22 g 2'-FL/g lactose, showing 1.72- and 1.51-fold increase in the titer and yield, respectively, compared to those of the control strain. The simple and powerful method developed in this study could be applied to enhance the solubility of other unstable enzymes.

• 한국작물학회지
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• 제31권1호
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• pp.19-23
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• 1986

27개 농가포장에서 인삼종자의 생산량 및 종자소질을 조사하였던 바, 그 결과를 요약하면 다음과 같다. 1. 포장별 채종량은 거의가 4~10$\ell$/간(간 : 180x90cm의 면적) 절위이였다. 2. 채종량이 많은 포장의 종자일수록 100입중, 4mm이하의 종자 생산 비율이 모두 낮아지는 경향이었다. 3. 전종자의 12%는 4mm이하의 종자이었으며 66%는 4-5mm, 22%는 5mm이하의 종자이었다. 4. 채종모본 선정 포장에서는 방임채종포에 비해 채종량은 감소되었으나 100입중, 4mm이하 종자의 생산비율은 오히려 증가되었다. 5. 종자의 길이와 폭 간에는 정(＋)상관이 인정되었으나 폭과 두께, 길이와 두께간에는 유의상관 인정되지 않았다. 6. 채종량은 채종모본의 생육상태에 따라 차이가 있었으나 4mm이하의 종자생산 비율을 감안할 때 100간당 7-8$\ell$ 미만 채종하는 것이 좋을 것으로 보여졌다.

• 무역상무연구
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• 제52권
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• pp.97-118
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• 2011

85% of South Korea's gross domestic product(GDP) depends on trade. Exports amount in 2011 exceeds $ 5,000Billion. Korea is expected to achieve $ 1 trillion in total trade volume and will become finally the ninth in the world. We do not have a lot of natural resources and are bound to export the finished products to pursue economic development. In other words, trade sector is very essential for Korea to continue economic growth. The department of trade in universities have brought up the concerned students serving for trade sectors. Currently, "half-price tuition" in universities has become very controversial issue in Korea so this paper studies the efficiency of "the Department" because the universities may reconsider the adjustment of tuition fee, if they can enhance the efficiency level. DEA model is used for the analysis. As a result, 8 DMUs like KAN1, KEI5, BAE10 etc. show relatively higher efficiency levels.

• International Journal of Control, Automation, and Systems
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• 제2권1호
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• pp.39-54
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• 2004

We developed an automatic blood pressure control system to maintain the blood pressure of patients at a substantially low level during a surgical operation. The developed system discharges two functions, continuous feedback control of the mean arterial pressure (MAP) by a state-predictive servo controller and risk control based on the inference by fuzzy-like logics and rules using measured data. Twenty-eight clinical applications were made beginning in November 1995, and the effects of the automatic blood pressure control on the operation time and on bleeding were assessed affirmatively by means of Wilcoxon testing. This paper essentially reports the engineering details of the control system.

• Journal of Advanced Marine Engineering and Technology
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• 제33권1호
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• pp.28-36
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• 2009

In this paper, based on the measured data of hull deflection, an approximated hull deflection curve is drawn using reverse analyzed hull deflection data and the estimation method for flexibility analysis of shaft alignment is proposed by use of the approximate hull deflection curve. Generally an offset value of after stern tube bearing is a datum point with an fore stern tube bearing however the shaft alignment has a tendency which is able to get higher flexibility if the shafting system has the deflection value from after stern tube bearing as reference to bottom direction according to results of on this study. By applying this result of study, the shaft alignment for next similar ships will be able to estimate how to follow the hull deflection and how to be influenced by hull deflection at shaft alignment analysis state using the approximated hull deflection curve.

• 건축역사연구
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• 제5권1호
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• pp.18-38
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• 1996

Dosan Sedans was built in 1560, when Toege I Whang(1500-1571) was 60, as a place in which to study and teach disciples. The building was built to a humble 3-kan system which was a popular form of sodang in the 16th century, There found an enlargement of space by attaching outer walls around three sides which was designed by Toege himself. Minimum in space, moderation in form, conrol of embellishment, hermit scholar's aethetics became basic concept of the architecture of Dosan Sodang. After completion of sodang, Toege wrote many poems about pond, walls, flower bed and natural surroundings like sheer cliff, winding river and even rural daily life of villagers around the building. It could be sud that ,for Toege, architecture was cogniged as a whole complex of building and its surrounding natural, artificial and human circumstances.

• Fibers and Polymers
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• 제6권3호
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• pp.229-234
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• 2005

In this paper, chitosan was suggested for using as a replacement for sodium alginate in the pretreatment print paste for digital ink-jet printing for cotton fabric. Pretreatment print pastes prepared from the mixture of chitosan and acetic acid with the appropriate viscosity gave satisfactory prints on the cotton fabric. Chitosan-treated cotton fabrics were digitally ink-jet printed with four different colors and the color fastness rating of the printed fabrics was satisfactory. Experimental results revealed the possibility of pre-treating the cotton with chitosan to replace the sodium alginate normally present in the pretreatment print paste recipe.