• BMB Reports
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• v.37 no.2
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• pp.229-233
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• 2004

A haemagglutinating protein from the saline extracts of Kalanchoe crenata leaves, which agglutinate all human blood types, was purified to homogeneity by ion-exchange chromatography on a DEAE-Cellulose column followed by gel filtration on a Sephadex G-100 column. The purified protein showed one band, both in non-denaturing PAGE and SDS-PAGE. The $M_r$ that was determined by SDS-PAGE was 44,000 Da and that estimated from gel filtration was 47,000. Treatment of the haemagglutinating protein with 5 mM EDTA diminished the haemagglutinating activity to 50% of the original level. The addition of divalent cations, 10 mM $Mg^{2+}$, 10 mM $Mn^{2+}$, or 10 mM $Ba^{2+}$, totally restored and enhanced the activity. The protein showed maximum activity over the 3-7 pH range and was heat-resistant. It was also a glycoprotein containing about 1.5% carbohydrate.

• Journal of the Korean Institute of Landscape Architecture
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• v.29 no.6
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• pp.92-100
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• 2002

This study was carried out to obtain fundamental information on the growth response of interior landscape plants under fluorescent light, sunlight and optical fiber lighting indoors. Saintpaulia ‘Delaware’, ‘Kalanchoe blossfeldiana, Anthurium scherzerianum and Ardisia crenata were examined using light intensity of 5001ux and 1,0001ux of fluorescent light, sunlight and optical fiber lighting in an interior environment. Results of experiments are as follows; 1) Plant growth status showed the best results under optical fiber lighting compared with fluorescent light or sunlight. 2) Plant growth status was better under 1,0001ux light intensity than 5001ux light intensity and in cases of the same light intensity, the highest growth increase was under optical fiber lighting. while it was showed relatively different according to the different plant species between a fluorescent light and sunlight. 3) The deep pinkish red color of Saintpaulia ‘Delaware’flower was obtained first under an optical fiber lighting and a fluorescent light, a sun light in that order. 4) Regarding interred activity, photosynthetic rate and transpiration rate, intercellular CO, water absorption rate showed a similar tendency generally in spite of a little difference. Namely, transpiration rate and intercellular CO, $CO_2$ a absorption rate increased according to increase of photosynthetic rate. 5) Photosynthetic rate of test plants except Anthurium scherzerianum increased according to increase of light intensity and increased highest under optical fiber lighting in the same light intensity condition. Increases differed under fluorescent light and sun light. That of Saineaulia ‘Delaware’and Anthurium scherzerianum increased in the order of optical fiber, fluorescent light and sun light, but that of Kalanchoe blossfeldiana and Ardisia pusilla increased in the order of optical fiber lighting, sun light and fluorescent light. Summing up these results, In visual value or internal health status of all experimental plants we obtained the highest result under an optical fiber lighting. Finally, we need to introduce an optical fiber lighting in interior landscape space as main light source.