• Food Science and Biotechnology
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• v.14 no.2
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• pp.286-289
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• 2005

Active antibacterial compound against periodontitis-causing bacterium Porphyromonas gingivalis was isolated from Kaempferia pandurata and identified as panduratin A. Minimum inhibitory concentration (MIC) value of panduratin A was $4\;{\mu}g/mL$, much lower than those of other natural antibacterial agents. Panduratin A also showed antibacterial activity against Prevotella intermedia ($2\;{\mu}g/mL$), P. loescheii ($4\;{\mu}g/mL$), and cariogenic Streptococcus mutans ($4\;{\mu}g/mL$). Damage on cell wall and perturbation of cytoplasmic membrane of panduratin A-treated P. gingivalis were visualized through transmission electron microscopy. These results suggest panduratin A, exhibiting strong and preferential antiperiodontal and anticariogenic activities, may be utilized in functional foods for prevention of oral diseases.

• Food Science and Biotechnology
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• v.17 no.6
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• pp.1357-1360
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• 2008

Propionibacterium acnes is the predominant organism in sebaceous regions of the skin and is thought to play an important role in the pathogenesis of inflamed lesions. Antibacterial compounds against P. acnes were isolated from the ethanol extract of Kaempferia pandurata Roxb. and identified as panduratin A and isopanduratin A. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of panduratin A for P. acnes were 2 and $4{\mu}g/mL$, respectively, while those of isopanduratin A were 4 and $8{\mu}g/mL$, respectively. The time-dependent killing effect showed that panduratin A and isopanduratin A completely inhibited the growth of P. acnes at 4 and $8{\mu}g/mL$ in 48 hr, respectively. Panduratin A and isopanduratin A demonstrated high antibacterial activities not only against P. acnes but also other skin microorganisms. The results suggest that panduratin A and isopanduratin A could be employed as natural antibacterial agents to inhibit the growth of acne and skin disease causing microorganisms.

• Natural Product Sciences
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• v.13 no.2
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• pp.110-113
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• 2007

Five flavonoid derivatives, pinostrobin (1), pinocembrin (2), alpinetin (3), cardamonin (4) and boesenbergin A (5) were isolated from the rhizomes of Boesenbergia pandurata. All compounds were elucidated based on its spectroscopic data and by the comparison with the previous works. 2D NMR technique was used for the structure elucidation of boesenbergin A to complement the data reported previously. The extracts and pure compounds were screened for cytotoxic activity against HL-60 cancer cell lines (human promyelocytic leukemia). Cytotoxic screening showed most of the extracts and pure compounds isolated from the rhizomes of Boesenbergia pandurata were active against HL-60 cancer cell line. The chloroform extract and boesenbergin A showed the most potent cytotoxic activity.