• Journal of Microbiology and Biotechnology
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• v.7 no.6
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• pp.417-422
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• 1997

Recombinant Bacillus subtilis LKS88[pASA240] containing the amylase gene from Streptomyces albus KSM-35 was exploited in fed-batch cultivation for mass production of maltotetraose-producing amylase. The effects of dissolved oxygen, additional organic nutrients (peptone and yeast extract) and mixed carbon sources (glucose plus soluble starch) on amylase production were examined in fed-batch operations in an effort to determine the optimum conditions for a maximum amylase productivity. Under the optimum conditions, maximum amylase activity was about 4.2 times higher than that obtained in batch cultivations, indicating that mass production of maltotetraose-producing amylase could be accomplished in fed-batch cultivation of the recombinant B. subtilis strain.

• Tribology and Lubricants
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• v.36 no.2
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• pp.75-81
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• 2020

In this paper, we present an experimental investigation of the leakage and endurance performances of mechanical face seals in a 75-tonf turbopump for the Korea Space Launch Vehicle II first-stage engine. A mechanical face seal is used between the fuel pump and turbine to prevent mixing of the fuel and turbine gas. However, excessive leakage occurs through the carbon attached to the mechanical face seal bellows. To reduce this leakage, we redesign the mechanical face seal such that the contact area between the fuel and carbon is reduced, height of the carbon nose is reduced, and stiffness of the bellows is increased. Then, we conduct static and dynamic leakage tests and endurance tests to compare the performances of the original and modified mechanical face seals. The investigation of the leakage of the old and new mechanical face seals confirms that the leakage performance is significantly improved, by 80%, in the new design in comparison with the old design. The endurance tests demonstrate that the average wear rate of carbon in the new mechanical face seal is 0.1094 ㎛/s. The service lifetime is predicted to be 4,200 s, which is 28 times greater than the requirement. Finally, we present a new mechanical face seal in a 75-tonf turbopump, and perform a validation test in the real-propellant test facility at the NARO Space Center. Based on the test results, we can confirm that the modified mechanical face seal works well under real operating conditions.

• Journal of the Korean Geotechnical Society
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• v.20 no.7
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• pp.107-117
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• 2004

A series of model tests were conducted to investigate how the number of reinforcement layers, stiffnesses, types of reinforcement material and buried depth of a flexible pipe can affect bearing capacity-settlement curve at a loose sand foundation. In the test results, whereas the type of failure in unreinforced sand was local shear, the type of failure, for model tests with more than 2 reinforcement layers in loose sand, was general shear: The number of the optimum reinforcement layers was found to be two: Stiffness and type of reinforcement were more important than the maximum tensile strength of reinforcement in improving bearing capacity. When the depth of buried pipe from the sand surface was less than the width of the footing, test results showed that both bearing capacity and ultimate bearing capacity of buried pipe in unreinforced sand significantly decreased, and the type of failure in the reinforced sand changed from general shear to local shear.

• Tunnel and Underground Space
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• v.23 no.6
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• pp.457-469
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• 2013

We have analyzed the regional in-situ stress pattern using 460 stress measurement data at about 100 test sites in Korea, and suggested correlation equations of stress-depth and stress ratio-depth. We made Korea Stress Map(KSM) as in-situ stress fields of the Korean peninsula, combining with a paleo-stress analysis according to the geological period and a stress estimation from focal mechanism. We confirmed the reliability and applicability of correlation equations derived in this study, comparing with worldwide stress-depth patterns, and also estimated the pattern of in-situ stress fields of north-eastern Asia including Korea, China and Japan, comparing with World Stress Map.

• Journal of the Korean GEO-environmental Society
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• v.14 no.6
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• pp.31-38
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• 2013

This study carried out fundamental study on the forest road pavement method of cementing the ground using the compaction equipment after laying by mixing with eco-friendly stabilizer, natural soil and water. Target strength of pavement was set to 2.0MPa and the specimen was produced per mixing ratio of cement, kinds of natural soil and curing period to evaluate the durability and unconfined compressive strength. Unconfined compressive strength test was conducted to compare strength by producing the test specimen mixing environment-friendly cement as well as the test specimen mixing cement with the same mixing ratio. To evaluate the durability, surface abrasion test and water flow resistance test were conducted. In addition, SB and GB tests were conducted using iron marble and golf ball to evaluate the walking satisfaction since it can be used by visitors due to the feature of forest road.

• Journal of the East Asian Society of Dietary Life
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• v.24 no.5
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• pp.529-543
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• 2014

The objectives of this study were to perform a descriptive analysis to characterize and compare the sensory properties of commercial Makgeolli products as well as a cross-cultural comparison of the sensory descriptions of these products between two sensory panels (Korean and Japanese). The samples used in this study were seven different types of Makgeolli, which were commercial products. A total of 10 Korean and 7 Japanese panelists were selected in Korea. Each group was trained, and they identified product attributes and performed descriptive analyses independently. The Korean and Japanese panelists generated 34 and 28 sensory attributes, respectively, to describe appearance, odor/aroma, taste/flavor, texture, and after flavor of the products. There were significant differences among the samples for 24 attributes by Korean and 23 attributes by Japanese panelists. Although there was not a large difference in the number of descriptors between Korean and Japanese panels, the Korean panelists generated more various attributes associated with flavor than the Japanese panelists, and the attributes of Japanese panelists included references to non-food products such as rotten grass. Multiple factor analysis (MFA) was applied to the descriptive analysis data from the Korean and Japanese panels to delineate the associations between Makgeolli samples and their sensory characteristics. Both the Korean and Japanese panels clustered the JRM, JSM, KRM and KSM samples into one group and the CRM and BSM samples into another group. The ESM sample was distinguished from the other six samples. These results of the cross-cultural comparison suggest that comparative analyses of sensory profiles between cultures should be conducted regularly in future studies, and further research such as consumer acceptance tests should be conducted to determine the sensory characteristics that drive consumer acceptance of Makgeolli products in the context of increasing food product exports to other countries.

• Transactions on Electrical and Electronic Materials
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• v.12 no.6
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• pp.253-257
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• 2011

This study was carried out to solve problems such as radio frequency band depletion, confusion risk, and security loss in existing visible wireless communication systems, and to determine the applicability of next-generation networks. A light-emitting diode (LED) light communication system was implemented with a controlling switching light module using the ATmega16 micro-controller. To solve the existing modulation effect and disturbance in visible light communication, an integrated interface was evaluated with a driving light module and analyzes its reception property. A transmitter/receiver using the ATmel's micro-controller, high-intensity white LED-6 modules, and infrared sensor KSM60WLM and visible sensor TSL250RD were designed. An experiment from the initial value of distance to 2.5 m showed 0.46 V of the voltage loss, and if in long distance, external light interference occurred and light intensity was lost by external impact and thus data had to be modified or reset repeatedly. Additionally, when we used 6 modules through the remote controller's lighting dimming, data could be transmitted up to 1.76 m without any errors during the day and up to 2.29 m at night with around 2~3% communication error. If a special optical filter can reduce as much external light as possible in the integrated interface, the LED for lighting communication systems may be applied in next generation networks.

• Journal of Microbiology and Biotechnology
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• v.31 no.10
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• pp.1446-1454
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• 2021

Herein, we cloned and expressed an endo-β-1,4-glucanase gene (celA1805) from Bacillus subtilis B111 in Escherichia coli. The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp. KSM-330. Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50℃, respectively, and it was stable at pH 3-9 and temperature ≤50℃. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity. Moreover, celA1805 showed a wide substrate specificity to CMC, barley β-glucan, lichenin, chitosan, PASC and avicel. The K_m and V_max values of celA1805 were 1.78 mg/ml and 50.09 µmol/min/mg. When incubated with cellooligosaccharides ranging from cellotriose to cellopentose, celA1805 mainly hydrolyzed cellotetrose (G4) and cellopentose (G5) to cellose (G2) and cellotriose (G3), but hardly hydrolyzed cellotriose. The concentrations of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw were increased by 0.21, 0.51, 0.26, 0.36, and 0.66 mg/ml, respectively. The results obtained in this study suggest potential applications of celA1805 in biomass saccharification.

• Journal of the Korean GEO-environmental Society
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• v.14 no.11
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• pp.65-71
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• 2013

Despite of the various merits of soil pavement, it has not been widely adapted because portland cement was conventionally used as soil stabilizer to improve the mechanical properties such strength parameters. Recently, natural soil stabilizer(NSS) were developed and virtually adopted to several case of soil pavement construction under control of heavy metal pollution compared to cement-used cases. However, the application of natural soil stabilizer is not settled yet, and empirical design have been widely adopted. In this study, therefore, the strength parameter of soil-NSS mixture was estimated by some triaxial compression tests, CU-test. From the tests, the relationship between curing period and strength parameter such as internal friction and effective cohesion was examined. As a result, effective cohesion of dredged clay and granite soil increased as curing time is increased. However, internal friction is almost same result in all soil type used in this study.

• Korean Journal of Food Science and Technology
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• v.30 no.6
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• pp.1426-1431
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• 1998

The research was undertaken to characterize enzymatic properties of Streptomyces albus amylase expressed in recombinant Bacillus subtilis. Molecular weight and pI of the purified enzyme were estimated to be 50 kD by SDS-PAGE and 4.3 by isoelectric focusing. The optimum temperature and optimum pH were $45^{\circ}C$ and 6.0, respectively. D-and Z-value were estimated to measure thermostability of the purified enzyme. The Z-value was estimated $17.7^{\circ}C$, which is lower than typical amylase. Maltotetraose was produced as a major component from soluble starch in the early state of reaction but gradually degraded to maltose. Thin layer chromatography was also performed to analyze the reaction products. The parameters involved in Michaelis-Menten enzyme kinetics were found to be the maximum velocity of 0.37 mM/min and the Michaelis constant of 0.13%, respectively.