• Title/Summary/Keyword: KSI

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Production and Amyloid fibril formation of tandem repeats of recombinant Yeast Prion like protein fragment

  • Kim, Yong-Ae;Park, Jae-Joon;Hwang, Jung-Hyun;Park, Tae-Joon
    • Journal of the Korean Magnetic Resonance Society
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    • v.15 no.2
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    • pp.175-186
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    • 2011
  • Amyloid fibrils have long been known to be the well known ${\alpha}$-helix to ${\beta}$-sheet transition characterizing the conversion of cellular to scrapie forms of the prion protein. A very short sequence of Yeast prion-like protein, GNNQQNY (SupN), is responsible for aggregation that induces diseases. KSI-fused tandem repeats of SupN vector are constructed and used to express SupN peptide in Escherichia coli (E.Coli). A method for a production, purification, and cleavage of tandem repeats of recombinant isotopically enriched SupN in E. coli is described. This method yields as much as 20 mg/L of isotope-enriched fusion proteins in minimal media. Synthetic SupN peptides and $^{13}C$ Gly labeled SupN peptides are studied by Congo Red staining, Birefringence and transmission electron microscopy to characterize amyloid fibril formation. To get a better understanding of aggregation-structure relationship of 7 residues of Yeast prion-like protein, the change of a conformational structure will be studied by $^{13}C$ solid-state nmr spectroscopy as powder of both amorphous and fibrillar forms.

Sliding Wear Behavior of Fe-Base Norem 02 Hardfacing Alloy in Pressurized Water (Fe계 Norem 02 경면처리 합금의 고압.수중 마모거동)

  • Lee, Kwon-Yeong;Oh, Young-Min;Lee, Min-Woo;Kim, Seon-Jin
    • Korean Journal of Materials Research
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    • v.12 no.8
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    • pp.608-612
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    • 2002
  • The sliding wear behavior of an iron-base NOREM 02 hardfacing alloy was investigated in the temperature range of $25~250^{\circ}C$ under a contact stress of 103MPa (15ksi). With increasing temperature, the wear loss of Norem 02 in water increased slightly up to $180^{\circ}C$ at which Norem 02 showed the wear loss of 2.1mg. The wear resistance of Norem 02 resulted from the surface hardening due to the strain-induced phase transformation from austenite to $\alpha$'martensite during sliding wear. The wear loss of Norem 02 was smaller in water compared to air at same temperature because the water could be served as a sort of lubricant. The wear mode of NOREM 02 changed abruptly to severe adhesive wear at $190^{\circ}C$ and galling occurred above $200^{\circ}C$. It was caused that the strain- induced phase transformation took place below $180^{\circ}C$ while not above $190^{\circ}C$. Therefore, Norem 02 was considered to be inadequate at high temperature service area.

A Study on the Retarding effects of Cememtn Mortar Setting (시멘트 모르타르의 응결 지연 효과에 관한 연구)

  • 이재한;이경희;김홍기
    • Journal of the Korean Ceramic Society
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    • v.33 no.3
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    • pp.307-312
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    • 1996
  • In following addition of 0.3, -0.6, 0.8, 1.0 and 5 weight percent MgSiF66H2O studies have been made of the setting and hardening characteristics of ordinary portland cement. MgSiF66H2O retarded the setting time of ordinary portland cement and extended the induction pariod of the hydration. In ordinary portland cement the setting characteristics were drastically altered especially at high MgSiF66H2O contents. Evidence was also obtained by the formation of a KSiF6 which was very fine particle. The results wee as follows. 1. Slump was slightly decreased when MgSiF66H2O added. 2. Setting time was retarded depending on the amount of retarding agent 2 to 8 hours 3. Compressive strength was almost same or some increased in comparision with opc. 4. When MgSiF66H2O was added to cement paste K2SiF6 were formed It was fine-sized distributed uniformly in cement grain and caused retardation of cement setting.

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A study of facial nerve grading system (안면신경기능의 평가방법에 대한 고찰;House-Brackmann scale이후의 New grade)

  • Kim, Mi-Bo;Kim, Ja-Hye;Shin, Sang-Ho;Yoon, Hwa-Jung;Ko, Woo-Shin
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.20 no.3
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    • pp.147-160
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    • 2007
  • Background and Objective : The facial nerve grading system proposed by House and Brackmann is most widely accepted for the clinical assessment of facial nerve injury. Because of the limitations and subjectivity of the House-Brackmann scale, several new scales of varying degrees of objectivity and ease of use have been introduced. To assess methods of evaluating the function of the facial nerve that have been introduced over the past 20 years, We compared with the House-Brackmann scale. Method : We referred to the information through Entrez Pubmed and Korean studies information(KSI) from 1985 to 2006 about methods of evaluating facial nerve function. We choose 7 scales that focused on objective and easy of use. Result and conclusion : Sunnybrook scale is a weighted, subjective scale with incorporation of secondary defects into a single composite score. Sunnybrook scale can be recommended over House-Brackmann scale.

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Case Study of Non-Metallic Repair Systems for Metallic Piping

  • Hammad, Bakr. S.
    • Corrosion Science and Technology
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    • v.7 no.1
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    • pp.6-12
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    • 2008
  • Non-metallic composite overwrap repair methods utilize resin based fiber-reinforced composite materials, which have higher specific strength to weight ratio and stiffness, superior corrosion and fatigue resistance, and substantially reduced weight when compared to carbon steel. Non-metallic repair methods/systems can allow desired functional properties to be achieved at a respectable economic advantage. For example, non-metallic composite repair systems have at least a 50 year design stress of 20 ksi and approximately 25% of the short term tensile strength of fiberglass. For these systems, the contribution of the repaired steel to the load carrying capability need not be considered, as the strength of the repair itself is sufficient to carry the internal pressure. Worldwide experience in the Oil & Gas industry confirms the integrity, durability, inherent permanency, and cost-effectiveness of non-metallic composite repair or rehabilitation systems. A case study of a recent application of a composite repair system in Saudi Aramco resulted in savings of 37% for offshore subsea line and 75% for onshore above grade pipeline job. Maintaining a pipeline can be costly but it is very small in comparison to the cost of a failure. Pipeline proponents must balance maintenance costs with pipeline integrity. The purpose is not just to save money but also to attain a level of safety that is acceptable. This technology involves the use of an epoxy polymer resin based, fiber-reinforced composite sleeve system for rehabilitation and /or repair pipelines.

Systematic Review of Cupping Including Bloodletting Therapy for Musculoskeletal Diseases in Korea

  • Cho, Hyeon-Joo;Kwon, Young-Dal
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.3
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    • pp.789-793
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    • 2007
  • To evaluate the effectiveness of cupping and bloodletting therapy in the treatment of musculoskeletal diseases. Systematic searches were conducted on KSI, KISTI, DB Pia, KIOM Database, and Koreamed until January 2007 Hand-searches included conference proceedings and our own files. There were no restrictions regarding the language of journals published in Korea. Controlled trials of dry cupping, wet cupping, or blood letting for patients with musculoskeletal disease were considered for inclusion. Trials testing other forms of dry cupping therapy were included. Methodological quality was assessed by two doctors. 20 possibly relevant studies were identified and 5 studies were included. One trial tested wet cupping for ankle sprain and reported positive result. Two trials tested blood letting for low back pain, one was positive and the other one was neutral. One trial tested the types of dry cupping for low back pain, and Ki-gong cupping therapy was superior to other two types of cupping. One trial compared wet cupping with dry cupping for low back pain and the result was negative. The effectiveness of bloodletting plus acupuncture for treating patients with low back pain is superior to acupuncture in spite of low quality. One trial of wet cupping for ankle sprain had effects in reducing pain. However, I suggest that the rigorous RCTs of cupping and blood letting therapy will be conducted in well designed features.

Study on storage change analysis of Samsan reservoir by applying calibrated soil parameters (토양 매개변수 보정에 따른 삼산저수지의 저류량 변화 분석)

  • Park, Sanghyun;Kim, Hyeonjun;Jang, Cheolhee;Birhanu, Dereje
    • Proceedings of the Korea Water Resources Association Conference
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    • 2019.05a
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    • pp.355-355
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    • 2019
  • 본 연구에서는 물리적 매개변수 기반의 물 순환 해석 모형인 CAT(Catchment hydrologic cycle Assessment Tool)을 이용하여 보령댐 유역 상류에 위치한 삼산저수지의 저류량을 분석하였다. 연구기간은 2012~2017년이며 2015년 발생한 가뭄 대책의 일환으로 2016년 이후 금강과 보령댐을 잇는 도수로를 설치하여 유역내로 외부유입량이 유입되었다. 이에 본 연구에서는 보다 정확한 저류량 산정을 위해 모의 기간을 2012~2015년 및 2016~2017년으로 나누어 연구를 수행하였다. 매개변수 보정에 앞서 CAT에서 제공하는 세 가지 침투해석방법인 Rainfall Excess, Green&Ampt, Horton 방법을 모두 적용하여 모의하였으며 그 결과 Rainfall Excess 침투방법을 적용했을 때의 모의 정확도가 비교적 높게 나타나는 것을 확인할 수 있었다. 이에 본 연구에서는 Rainfall Excess 침투방법의 주요 토양 관련 매개변수인 토양수분율(${\theta}s$), 연직방향 투수계수(ks) 및 사면방향 투수계수(ksi)를 대상으로 전역최적화기법(SCEUA-P)을 이용한 보정을 수행하였으며 보정 전 후의 모의 저류량과 관측 저류량을 연도별로 비교 및 분석하였다.

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Community Dynamics of Benthic Macroinvertebrates and Biological Evaluation of Water Quality from Gigyecheon (기계천의 저서성대형무척추동물 군집동태와 생물학적 수질평가)

  • Kim, Jung-Lark
    • Journal of Environmental Science International
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    • v.27 no.11
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    • pp.1007-1021
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    • 2018
  • Community dynamics of benthic macroinvertebrates and biological evaluation of water quality were investigated at Gigyecheon of Pohang and Gyeongju, Korea. The survey was carried out at 8 sites from May to October, 2017. As a result, total of 104 species under 54 families, 15 orders, 8 classes in 4 phyla with 5,052 individuals were revealed. The number of species was the most abundantly identified in aquatic insects, 76 species under 34 families, 6 orders. Among them, the order Odonata was the most abundant taxon as 21 species(20.2%). The most abundant individuals were collected in the phylum Mollusca as 1,453 individuals(28.8%). Various species appeared dominantly at each site by season, and the most dominant species was Caridina denticulata denticulata belonging to the class Malacostraca with 0.08 of dominance index. According to the results of community structure analyses, the diversity and richness indices were the highest at site II, the evenness index was the highest at site I, and the dominance index was the highest at site IV. The number of species and individuals of gathering collectors were the highest among functional feeding groups. Those of sprawlers were the highest among habitat orientation groups. The ESB showed a tendency to change according to season and site, and it was the highest in May at most surveyed sites. The KSI was also somewhat fluctuated according to season and site. From these indices, average biological water quality was evaluated as class II.

Comparative Analysis on the Application of Biotic Indices for Environmental Assessment of a Polluted Stream (Jinwi Stream) (오염하천(진위천)의 환경평가를 위한 생물지수간 적용성 비교분석)

  • Oh, Min Woo;Lee, Ok-Min;Song, Ho-Bok;Park, Sun Jin;Song, Mee Young;Kong, Dongsoo
    • Journal of Korean Society on Water Environment
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    • v.27 no.6
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    • pp.760-768
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    • 2011
  • Jinwi Stream is considerably polluted. The urban development in a Jinwi Stream basin can make state of aquatic ecosystem worse. However, researches for aquatic ecosystems in Jinwi Stream are insufficient. In this study, biotic indices of periphytic diatoms (DAIpo and TDI), benthic macroinvertebrates (EPT, KSI and ESB) and fish (IBI) were compared with the annual water quality data. Benthic macroinvertebrates indices showed highly significant correlations with concentrations of organic materials and nutrients, while DAIpo, TDI and IBI showed low correlations with them. In particular, ESB can be considered as an useful indicator that reflects the degree of diversity and abundance of biotic community as well as water quality. In polluted and disturbed streams as Jinwi Stream, DAIpo, TDI and IBI appeared to be not available for evaluating and discriminating the water quality, although they have been known as good indices in general streams.

Fitness-for-Service Assessment for Instrument Valve Material by Tensile Test for Verification (계기용 밸브 재질의 검증 인장시험에 의한 사용적합성 평가)

  • In Hwan Shin;Chi Yong Park
    • Transactions of the Korean Society of Pressure Vessels and Piping
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    • v.17 no.1
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    • pp.49-55
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    • 2021
  • In this paper, an application example for fitness-for-service of material is shown. A kind of instrument valve is made of austenitic stainless steel fabricated by the cold working process. The tensile strength of the cold worked austenitic stainless steel has to be limited under 90 ksi to prevent the stress corrosion cracking in power plants. In industrial fields, tensile strengths of some materials were discovered to be over the regulation requirements in a certified material test report (CMTR). Owner's verification tests were performed to compare with that in a CMTR and to check the appropriateness. It is found that, in the case of verification test under the required test speed, valve materials could be used in the field. Although it is only one application sample of material check process in the power generation site, this case study could show an importance of basic experimental technology in academia and research circles.