• Journal of Microbiology and Biotechnology
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• v.30 no.5
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• pp.700-707
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• 2020

The root-knot nematode (Meloidogyne incognita) is an important pathogen in crop cultivation, however, few methods are available to control this parasitic roundworm. In this study, the nematicidal effects of approximately 30 Streptomyces strains isolated from soil samples of Mt. Naejang (Korea) were tested against Meloidogyne incognita, and the culture broth of the strains KRA-24 and KRA-28 exhibited approximately 75% and 85% insecticidal activity, respectively, in in vitro assays. In in vivo pot experiments, these strains reduced the number of nematodes in the soil and the number of egg masses in the roots of red peppers. The two strains also survived in the presence of insecticidal agents (0.1 to 3.0%) such as fosthiazate, ethoprophos and terbufos when they were used in parallel. The mixture of KRA-24 or KRA-28 culture broth and fosthiazate exhibited nematicidal effects that were similar to those observed when KRA-24 or KRA-28 were used alone. Our results clearly suggest that the Streptomyces strains KRA-24 and KRA-28 should be promoted as a biocontrol agent against Meloidogyne incognita.

• BMB Reports
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• v.40 no.3
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• pp.315-324
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• 2007

The novel $\alpha$-amylase purified from locally isolated strain, Bacillus sp. KR-8104, (KRA) (Enzyme Microb Technol; 2005; 36: 666-671) is active in a wide range of pH. The enzyme maximum activity is at pH 4.0 and it retains 90% of activity at pH 3.5. The irreversible thermoinactivation patterns of KRA and the enzyme activity are not changed in the presence and absence of $Ca^{2+}$ and EDTA. Therefore, KRA acts as a $Ca^{2+}$-independent enzyme. Based on circular dichroism (CD) data from thermal unfolding of the enzyme recorded at 222 nm, addition of $Ca^{2+}$ and EDTA similar to its irreversible thermoinactivation, does not influence the thermal denaturation of the enzyme and its Tm. The amino acid sequence of KRA was obtained from the nucleotide sequencing of PCR products of encoding gene. The deduced amino acid sequence of the enzyme revealed a very high sequence homology to Bacillus amyloliquefaciens (BAA) (85% identity, 90% similarity) and Bacillus licheniformis $\alpha$-amylases (BLA) (81% identity, 88% similarity). To elucidate and understand these characteristics of the $\alpha$-amylase, a model of 3D structure of KRA was constructed using the crystal structure of the mutant of BLA as the platform and refined with a molecular dynamics (MD) simulation program. Interestingly enough, there is only one amino acid substitution for KRA in comparison with BLA and BAA in the region involved in the calcium-binding sites. On the other hand, there are many amino acid differences between BLA and KRA at the interface of A and B domains and around the metal triad and active site area. These alterations could have a role in stabilizing the native structure of the loop in the active site cleft and maintenance and stabilization of the putative metal triad-binding site. The amino acid differences at the active site cleft and around the catalytic residues might affect their pKa values and consequently shift its pH profile. In addition, the intrinsic fluorescence intensity of the enzyme at 350 nm does not show considerable change at pH 3.5-7.0.

• Journal of Veterinary Clinics
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• v.36 no.5
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• pp.292-295
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• 2019

A 2-year-old, 440 kg, thoroughbred gelding showed a lameness grade of 3 out of 5, as well as swelling and heating on the proximal interphalangeal joint (PIPJ). Periarticular new bones due to the arthritis were observed on performing radiography and ultrasonography. PIPJ arthrodesis was performed under general anesthesia. With the PIPJ open, the articular cartilage was removed by using curette, chisel, and mallet. Arthrodesis was performed with a PIP plate and two additional transarticular cortex screws inserted in lag fashion. A cast was placed over the limb distal from the proximal metatarsal bone. At 6 months after surgery, complete union of the PIPJ was confirmed by radiography, and the incision site was well closed. Based on the outcome, a PIP plate with two additional transarticular cortex screws inserted in lag fashion is recommended as a method for treating osteoarthritis of the PIPJ in horses.

• Journal of Veterinary Clinics
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• v.35 no.3
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• pp.107-110
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• 2018

A 5-year-old Thoroughbred race horse was presented to Busan Korea Racing Authority equine hospital with a 3-year history of a slow-growing left rostral maxillary mass. The location and progressive growth of the mass eventually resulted in poor food prehension, quidding and mouth bit placement. The mass was solitary and hard, and covered by normal smooth oral mucosa. Radiographic examination of the maxillae showed a flocculated and mixed radiolucent lesion protruding outward and displacing the 202 and 203 teeth caudally. The 202 tooth was in normal size and the 203 tooth was hypoplastic on radiography. Under general anesthesia, a partial surgical resection of the mass was performed to minimize functional loss and facilitate prompt return to track. After surgery, there was improvement in food intake, mouth bit placement, and cosmetic appearance. Histopathological examination determined the resected maxillay mass to be an ossifying fibroma. However, there was continued growth of remnant mass in the maxilla. Equine ossifying fibroma is a rare condition and primarily affects the rostral mandible, and less commonly, the maxillae. In this case, the lesion was slow-growing, and caused cosmetic and functional impairments, including poor food intake and reduced trainability. Surgical resection was performed, but the effect of treatment was limited due to advanced size / stage of the tumor. Early dental care is suggested for horse owners to prevent belated identification and improve successful treatment of oral disorders like ossifying fibroma.

• Journal of Information Technology Applications and Management
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• v.12 no.4
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• pp.157-171
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• 2005

Recently companies are aggressively Pushing ahead with integrating the systematical applications of internal and external information systems such as Data level, Application level, Process level in the company, Process level between companies. The EAI solution is generally considered as the necessary tool to integrate companies, but the appraisal standard for the EAI solution has not Vet settled in Korea, so the companies have difficulty in deciding whether to adopt the EAI solution or not. Through this report, we first introduce the KRA Project as the best practice in making an application of the settled standard to evaluate and adopt the EAI solution, and then suggest that it can be adapted to companies considering applying to the EAI solution.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2004.10a
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• pp.158-169
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• 2004

Recently companies are aggressively pushing ahead with integrating the systematical applications of internal and external information systems such as Data level, Application level, Process level in the company, Process level between companies. The EAI solution is generally considered as the necessary tool to integrate companies, but the appraisal standard for the EAI solution has not yet settled in Korea, so the companies have difficulty in deciding whether to adopt the EAI solution or not. Through this report, we first introduce the KRA Project as the best practice in making an application of the settled standard to evaluate and adopt the EAI solution, and then suggest that it can be adapted to companies considering applying to the EAI solution.

• 월간낙농육우
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• v.26 no.4 s.288
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• pp.179-183
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• 2006

슈퍼젖소탄생/ 국내 젖소검정우 세계4위 등재/ '한우 둔갑' 수입쇠고기 적발 가능해져/ DDA · FTA 개방대응 기금 조성/ DDA협상 난항, 회원국간의 입장차 커/ 한 · 미 FTA 대응체제 재정비 '탄력'/ 한우 가격 '600kg 기준' 발표/ 축단협 회장, 남호경 한우협회장 재추대/ 축산분뇨 활용 자연순환형 농업시급/ 소비자단체, '쇳가루 분유' 제조사와 수입업체 대상 소송 제기/ 농지내 축사 신축 더 어려워졌다/ 美 광우병 '전방위' 대응/ 작년 축산물 시장 외산 '폭증'/ 낙농가 '품질관리 시스템' 본격가동/ KRA, 특별적립금 843억 편성/ 국내산마 경매 최고가 경신/ KRA와 함께하는 농촌희망재단, 1학기 장학금 21억 지원/

• Research in Plant Disease
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• v.22 no.4
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• pp.227-235
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• 2016

Plant disease caused by root-knot nematode is a major problem in crop production. Using of chemical pesticides, one of the most efficient methods to control nematodes, have raised issues in toxicity to humans and animals and environmental pollution. In this study, to select actinomycete strains that have potential to serve as a microbial agent for control of nematodes, we investigated nematicidal activity of culture broth from 670 Streptomyces isolates. A culture filtrate of KRA15-528 isolate that was identified as S. flavogriseus on the basis of 16S rRNA sequence analysis, showed strong nematicidal activity against second stage of juveniles of Meloidogyne incognita and inhibited egg hatching; exposure to 10% of culture filtrate resulted in 71% juvenile mortality at 48 hours afters treatment and suppressed egg hatching by 54% at 9 days after treatment. When the KRA15-528 culture filtrate was partitioned with ethyl acetate and butanol, ethyl acetate layer exclusively showed strong activity; 91%, 53%, 30% of mortality at 1,000, 500, $250{\mu}g/ml$, respectively. Additionally, the culture filtrate suppressed gall formation on cucumber plant by M. incognita with no phytotoxicity. These results suggest that S. flavogriseus KRA15-528 has potential to serve as a microbial nematicide for the control of root-knot nematode disease.

• Journal of Veterinary Clinics
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• v.38 no.5
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• pp.225-230
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• 2021

A 4-year-old gelding Thoroughbred racehorse, which had been undergoing antibiotic therapy at a local veterinary clinic, was referred to the KRA veterinary center with a 20-day history of continuous right nasal discharge. Patient's history, endoscopic examination, and radiographic examination revealed primary maxillary sinusitis. Under sedation, surgical intervention was performed to collect samples and remove the accumulated mucopurulent exudate in the sinus. Swab samples were collected from the sinus during surgery for cytology and antimicrobial susceptibility testing. Only one type of bacteria was cultured, and molecular analyses of 16S ribosomal RNA gene sequences identified it as Staphylococcus aureus (S. aureus). The isolate was resistant to multiple antibiotics, which are frequently used in equine practice. Trimethoprim-sulfamethoxazole was chosen based on antibiotic susceptibility test, trephination, and sinus lavage using saline were applied to treat bacterial sinusitis. The clinical signs improved after 1 month and the patient resumed training. This report describes S. aureus isolated from bacterial maxillary sinusitis in a horse and its antibiotic susceptibility.

• Proceedings of the Korea Information Processing Society Conference
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• 2013.11a
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• pp.864-867
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• 2013

키 복구 시스템은 1994년 미국 NIST의 키 복구 제품 EES 개발이후 많은 나라에서 활발한 연구가 이루어지고 있는 분야이다. 국내에서도 단일 키 복구 시스템부터 다중 키 복구 시스템을 바탕으로 다양한 키 복구 방식들이 제안되고 있다. 하지만 기존 방식은 단일, 다중 기관시스템에 있어서 낮은 안전성의 문제점을 가지고 있다. 본 논문에서는 기존의 키 복구 시스템을 분석하고 다중 키 복구 기관을 기반으로 키를 N개로 나누어서 분산 보관하며, 키 복구 기관의 기능 상실, 결탁 등 문제에도 키 복구가 가능하도록 설계 하였다. 또한 ElGamal 방식을 이용하여 키 위탁, 키 복구, 합법적인 감청 절차를 제안하였다.